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Soberania e representa??o: Hobbes, parlamentaristas e levellers
Ostrensky, Eunice;
Lua Nova: Revista de Cultura e Política , 2010, DOI: 10.1590/S0102-64452010000200007
Abstract: the article intends to show that thomas hobbes (1588-1679) adopted the concept of political authorization as means to fight against the doctrine of the accountability of political actors ahead of the persons they represented. among these opponents, the levellers have a prominent place, by proposing, in the late 1640s, an innovative solution for the constitutional problem of the illegitimacy of the english parliament: extension of the franchise followed by a celebration of an agreement of the people.
As várias faces de um ator político
Revista latinoamericana de filosof?-a , 2011,
Abstract: this article aims at showing that the political science of james harrington (1611-1677) was intended to create a complex republican model as a remedy to the constitutional crisis in england during the 1650's. but, before presenting his model of republic to his contemporaries, harrington thought it was necessary to investigate why sovereign power collapsed in 1640. his starting point was rather empirical than speculative or abstract. despite the decisive part played in harrington's political science by foundations or material relations based on freehold property, human agency was also considered altogether necessary to produce a perfect political order.
As várias faces de um ator político
Eunice Ostrensky
Revista Latinoamericana de Filosofía , 2011,
Abstract: En este artículo se pretende demostrar que la ciencia política de James Harrington (1611-1677) tenía la intención de crear un complejo modelo republicano como solución a la crisis constitucional en Inglaterra durante la década de 1650. Pero, antes de presentar a sus contemporáneos su modelo de república, Harrington consideró necesario investigar por qué el poder soberano se rompió en 1640. Su punto de partida era más bien empírico que especulativo o abstracto. A pesar de que las fundaciones o relaciones materiales basados en la libre propiedad ejercieran un rol decisivo en la ciencia política de Harrington, la acción humana seria imprescindible para producir un orden político perfecto. This article aims at showing that the political science of James Harrington (1611-1677) was intended to create a complex republican model as a remedy to the constitutional crisis in England during the 1650's. But, before presenting his model of republic to his contemporaries, Harrington thought it was necessary to investigate why sovereign power collapsed in 1640. His starting point was rather empirical than speculative or abstract. Despite the decisive part played in Harrington's political science by foundations or material relations based on freehold property, human agency was also considered altogether necessary to produce a perfect political order.
Lethargic crab disease: multidisciplinary evidence supports a mycotic etiology
Boeger, Walter A;Pie, Marcio R;Ostrensky, Antonio;Patella, Luciana;
Memórias do Instituto Oswaldo Cruz , 2005, DOI: 10.1590/S0074-02762005000200009
Abstract: although lethargic crab disease (lcd) is causing massive mortalities in populations of the mangrove crab ucides cordatus of northeastern brazil, the identity of its etiological agent was hitherto unknown. in this study we provide robust evidence suggesting that lcd is caused by an anamorph ascomycota (fungi). we examined specimens of u. cordatus collected from stocks affected by lcd. histological and tem methods detected the presence of hyphae, conidia, and condiophores in several host tissues. moreover, the abundance of fungal stages is negatively associated with crab health. finally, dna was isolated from the fungus and a region of its 18s ribosomal gene was sequenced. phylogenetic analyses not only confirm the diagnosis of the lcd fungus in crab tissues as an ascomycete, but also suggest a close relationship with members of the subphylum pezizomycotina.
Detection of Mycobacterium in clinical samples by multiprimer polymerase chain reaction
Barouni, Abdulmonam Saied;Saridakis, Halha Ostrensky;Vidotto, Marilda Carlos;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000100004
Abstract: we investigated the use of multiprimer-pcr for detection of mycobacteria species in clinical samples. three different mycobacterial genomic fragments were investigated: the is6110 insertion sequence, present in m. tuberculosis complex; the genus specific fragment (32kda); and from m. tuberculosis species-specific mtp40 gene. the sensitivity and specificity using 135 clinical isolates were 94.5% and 95.9%, respectively, compared with culture in l?wenstein-jensen medium; the detection limit was 0.05ng of dna. in conclusion, this assay is reliable and rapid for detection of mycobacterium species in clinical samples, and differentiates m. tuberculosis from m. bovis strains in a single-step assay.
Prospec??o do molusco invasor Limnoperna fortunei (Dunker, 1857) nos principais corpos hídricos do estado do Paraná, Brasil
Pestana, Debora;Ostrensky, Antonio;Tschá, Marcel Kruchelski;Boeger, Walter A.;
Papéis Avulsos de Zoologia (S?o Paulo) , 2010, DOI: 10.1590/S0031-10492010003400001
Abstract: were collected in the main water bodies of the state of paraná (brazil) it will seek the golden mussel larvae, limnoperna fortunei (dunker, 1857). this species of mussel is colonizing various water bodies in south america since its introduction in argentina in 1991. already reached the paraná and paraguay rivers, having reported their presence in the states of mato grosso do sul, paraná, sao paulo, minas gerais and rio grande do sul. samples were collected in winter and spring of 2007 and summer and autumn 2008 in 14 sampling points along the rivers igua?u, piquiri, ivaí (tributaries of the paraná river) and tibagi (tributary of the paranapanema river). this study determined the presence of this species throughout all parts of the igua?u river, in two sites of piquiri and a sample point of the tibagi river. in the ivaí river, their presence was not detected at any point in any season. based on the results, was made a map of occurrence of the species in the state of paraná. these results are useful to base a decision concerning the advancement of the species in order to monitor their presence and/or prevent their entry into other water bodies in the state.
Screening and confirmatory assays for detection of ESBLs (extendedspectrum -lactamases) production by Klebsiella pneumoniae isolates Métodos de triagem e de confirma o para detec o de BLEAs (beta lactamases de espectro ampliados) em isolados de Klebsiella pneumoniae
Eliana Carolina Vespero,Marcia Regina Eches Perugini,Halha Ostrensky Saridakis
Semina : Ciências Biológicas e da Saúde , 2007,
Abstract: ESBLs (extended-spectrum -lactamases) are enzymes produced by Enterobacteriaceae, especially Klebsiella pneumoniae, which mediate resistance to extended-spectrum -lactams and aztreonam. Considering the importance of detecting ESBLs producing strains, in this study, usual screening and confirmatory assays were compared. One hundred and seven strains of K. pneumoniae, isolated from patients with urinary tract infection, were screened for ESBLs production according to NCCLS 2005 guidelines, using broth microdilution in the Microscan Walkaway (Dade Behering) and Kirby-Bauer disk diffusion (KBD) test. Fifty-five strains selected as possible ESBL producers (51,4%), were submitted to confirmatory assays: E-test, double disk (DD) approximation and clavulanic acid (CA) association. Production of ESBLs was detected by the five methods used in 38 (69%) strains and 10 (18,2%) were negative only in DD. Seven strains (12,7%) did not present suggestive breakpoints in the Microscan Walkaway and were negative in the DD test, whereas the KBD values for the same strains obtained were indicative of ESBLs production. These results suggest that the combination of KBD breakpoints determination and CA association can be used as an efficient, alternative and less expensive system than the E-test. BLEAs ( -lactamases de espectro estendido) s o enzimas produzidas por várias espécies de Enterobacteriaceae, principalmente Klebsiella pneumoniae, as quais conferem resistência aos lactamicos de espectro ampliado e aztreonam. Considerando a importancia da detec o de BLEAs, neste estudo foram comparados métodos de triagem e de confirma o. As 107 amostras de K. pneumoniae, isoladas de pacientes com infec o urinária foram triadas quanto à produ o de BLEAs, segundo normas do NCCLS 2005, utilizando microdilui o em caldo no Microscan Walkaway e teste de disco difus o de Kirby-Bauer (KBD). As 55 cepas selecionadas foram submetidas a ensaios de confirma o como possíveis produtoras de BLEAs (51,4%): E-test, aproxima o com discos duplos (DD) e associa o com ácido clavulanico (CA). A produ o de BLEAs foi detectada pelos cinco métodos em 38 (69%) amostras e 10 amostras (18,2%) foram negativas para o teste DD. Sete amostras (12,7%) n o apresentaram valores sugestivos no método automatizado e também foram negativos no teste de DD, mas os valores dos halos no teste de KBD foram indicativos da produ o de BLEAs. Estes resultados sugerem que o uso combinado do método KBD e associa o com CA podem ser utilizados como um sistema alternativo eficiente e menos dispendioso que o E-test.
Shiga toxin-producing Escherichia coli (STEC): principal virulence factors and epidemiology Escherichia coli produtora de toxina shiga (STEC): principais fatores de virulência e dados epidemiológicos
Ariane Mayumi Saito Bert?o,Halha Ostrensky Saridakis
Semina : Ciências Biológicas e da Saúde , 2007,
Abstract: Shiga toxin producing Escherichia coli is an important food borne pathogen, mainly beef products, and is associated to mild and severe bloody diarrhea. In some individuals, STEC infection can progress to hemolytic-uremic syndrome (HUS), a sequela characterized by renal failure, and thrombotic thrombocytopenic purpura (TTP), with possible central nervous system involvement. Cattle, usually healthy, is the principal reservoir of STEC, although these strains have also been isolated from other domestic animals: sheep, goats, dogs, cats and pigs. The principal virulence feature, the production of Shiga toxins, is not enough to cause diseases, and other factors are considered important, as enterohemolysin and fimbrial and afimbrial adhesions production. Although human diseases associated to STEC have not been frequently reported in Brazil, their presence is frequent in cattle, as well as the correlation between serotypes found in these animals and human patients. Escherichia coli produtora de toxina Shiga (STEC) é um importante patógeno veiculado por alimentos, principalmente produtos derivados de carne bovina e está associado a quadros de diarréias leves a severas e sanguinolentas. Em alguns indivíduos, a infec o por STEC pode progredir para a síndrome hemolítico-urêmica (HUS), seqüela caracterizada pela falência renal e a púrpura trombocitopênica trombótica (TTP), com possível envolvimento do sistema nervoso central. O gado bovino, geralmente saudável, é o principal reservatório de STEC, embora estas cepas também tenham sido isoladas de outros animais domésticos: ovelhas, cabras, c es, gatos e suínos. A principal característica de virulência, a produ o de toxinas Shiga, n o é suficiente para causar doen as e outros fatores s o considerados relevantes, como a produ o de enterohemolisina e de adesinas fimbriais e afimbriais. Embora as doen as humanas associadas a STEC sejam pouco descritas no Brasil, podemos observar uma significativa ocorrência destas cepas nos rebanhos bovinos, bem como a correla o entre sorotipos encontrados nestes animais e em pacientes humanos.
The effect of temperature and body size on filtration rates of Limnoperna fortunei (Bivalvia, Mytilidae) under laboratory conditions
Pestana, Débora;Ostrensky, Antonio;Boeger, Walter Antonio Pereira;Pie, Marcio Roberto;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000100018
Abstract: the golden mussel (limnoperna fortunei, mollusca: bivalvia) is an invasive species that has been causing considerable environmental and economic problems in south america. in the present study, filtration rates of l. fortunei were determined in the laboratory under different temperatures (10, 15, 20, 25, 28, and 30 oc) and two types of food (algamac-2000? and the chlorophycean alga scenedesmus sp.). there was a statistically significant relationship between time and filtration rates in the experiment using scenedesmus sp., regardless of temperature. however, this pattern was absent in the experiment using algamac, suggesting that the relationship between filtration rates and temperature might depend on the size of the filtered particles. in addition, there was no correlation between filtration rates and either shell size or condition index (the relationship between the weight and the length of a mussel). the filtration rate measured in the present study (724.94 ml/h) was one of the highest rates recorded among invasive bivalves to date. given that the colonies of the golden mussel could reach hundreds of thousands of individuals per square meter, such filtration levels could severely impact the freshwater environments in its introduced range.
Survival and conjugal transfer between Bacillus thuringiensis strains in aquatic environment
Furlaneto, Luciana;Saridakis, Halha Ostrensky;Arantes, Olívia Márcia Nagy;
Brazilian Journal of Microbiology , 2000, DOI: 10.1590/S1517-83822000000400001
Abstract: field and laboratory studies were conducted to assess the survival of cells and spores and plasmid transfer between bacillus thuringienis strains in aquatic environment. results indicated that cells and spores of b. thuringiensis can survive for 10 days in water, without altering their number. the sporulation process began after 12-15 hours of inoculation of water. b. thuringiensis was able to transfer conjugative plasmids in the aquatic environment.
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