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A Unified Theory (I) for Neighborhood Systems and Basic Concepts on Fuzzifying Topological Spaces  [PDF]
Osama Rashed Sayed
Applied Mathematics (AM) , 2012, DOI: 10.4236/am.2012.39146
Abstract: This paper considers fuzzifying topologies, a special case of I-fuzzy topologies (bifuzzy topologies), introduced by Ying. It investigates topological notions defined by means of -open sets when these are planted into the frame-work of Ying’s fuzzifying topological spaces (by Lukasiewicz logic in [0, 1]). In this paper we introduce some sorts of operations, called general fuzzifying operations from P(X) to , where (X, τ) is a fuzzifying topological space. By making use of them we contract neighborhood structures, derived sets, closure operations and interior operations.
Mutations underlying 3-Hydroxy-3-Methylglutaryl CoA Lyase deficiency in the Saudi population
Moeenaldeen Al-Sayed, Faiqa Imtiaz, Osama A Alsmadi, Mohammed S Rashed, Brian F Meyer
BMC Medical Genetics , 2006, DOI: 10.1186/1471-2350-7-86
Abstract: We utilized Whole Genome Amplification (WGA), PCR and direct sequencing to identify mutations underlying 3HMG in the Saudi population. Two patients from two unrelated families and thirty-four 3HMG positive dried blood spots (DBS) were included.We detected the common missense mutation R41Q in 89% of the tested alleles (64 alleles). 2 alleles carried the frame shift mutation F305fs (-2) and the last two alleles had a novel splice site donor IVS6+1G>A mutation which was confirmed by its absence in more than 100 chromosomes from the normal population. All mutations were present in a homozygous state, reflecting extensive consanguinity. The high frequency of R41Q is consistent with a founder effect. Together the three mutations described account for >94% of the pathogenic mutations underlying 3HMG in Saudi Arabia.Our study provides the most extensive genotype analysis on 3HMG patients from Saudi Arabia. Our findings have direct implications on rapid molecular diagnosis, prenatal and pre-implantation diagnosis and population based prevention programs directed towards 3HMG.3-Hydroxy-3-Methylglutaric aciduria (3HMG, McKusick 246450) is an autosomal recessive branched chain organic aciduria caused by deficiency of the enzyme 3-Hydroxy-3-Methylglutaryl CoA lyase (HL, HMGCL, EC 4.1.3.4). This mitochondrial enzyme catalyzes the last step of both leucine degradation and ketogenesis[1]. Patients present in the neonatal or infantile period with severe hypoketotic hypoglycemia, metabolic acidosis, hyperammonemia, vomiting and hypotonia [2]. Untreated, this may progress rapidly to coma and death or may result in permanent neurological damage. With treatment, many patients do well although recurrent metabolic decompensation continues to occur especially with prolonged fasting and inter-current infections. Although a rare disease in Europe and Japan, it is reported to be common in Saudi Arabia and Portugal [3-5].Rapid biochemical diagnosis by blood acylcarnitine analysis on DBS using
Novel mutations underlying argininosuccinic aciduria in Saudi Arabia
Faiqa Imtiaz, Moeen Al-Sayed, Danyah Trabzuni, Bashair R Al-Mubarak, Osama Alsmadi, Mohamed S Rashed, Brian F Meyer
BMC Research Notes , 2010, DOI: 10.1186/1756-0500-3-79
Abstract: We utilized Whole Genome Amplification (WGA), PCR and direct sequencing to identify mutations underlying ASAuria cases diagnosed by our institution. A missense mutation that accounts for 50% of Saudi ASAuria patients was recently reported by our laboratory. In this study we report a further six novel mutations (and one previously reported) found in Saudi patients with ASAuria. The novel four missense, one nonsense and one splice-site mutation were confirmed by their absence in >300 chromosomes from the normal population. Pathogenicity of the novel splice-site mutation was also confirmed using reverse transcriptase-PCR analysis. Cross species amino acid conservation at the substituted residues described were observed in some but not all instances.Together, the eight mutations described by our laboratory, encompass >90% of ASAuria patients in Saudi Arabia and add to about 45 other ASAuria mutations reported worldwide.Argininosuccinic aciduria (ASAuria; OMIM 207900) or argininosuccinate lyase (ASL; EC 4.3.2.1) deficiency is an autosomal recessive inborn error of the urea cycle. ASL catalyses the reversible cleavage of argininosuccinic acid (ASA) into arginine and fumarate. ASL is a homotetramer of 50-kDa subunits and is found in the cytosol of hepatoctyes, renal cells and fibroblasts. Neonatal presentation of ASAuria is most common and is characterized by lethargy, vomiting, hypothermia, decreased consciousness and coma usually occurring between 24 and 72 hrs. Biochemical analysis reveals hyperammonemia, elevated plasma citrulline levels and increased ASA in dried blood spots (DBS), plasma and urine[1].The ASL gene is located on chromosome 7q11.2, and is approximately 35 kb in length and spans 17 exons (16 coding). Mutations in ASL have been shown to cause ASAuria but due to substantial clinical and genetic heterogeneity reported in this disease, a clear genotype/phenotype correlation has not been clearly defined [2-10]. Recently, an ASL pseudogene was reported to be l
Developing Business Management Students' Persuasive Writing Through Blog-based Peer-Feedback
Osama Hassanein Sayed
English Language Teaching , 2010, DOI: 10.5539/elt.v3n3p54
Abstract: The present study attempted to investigate the effect of using blog-based peer feedback on the persuasive writing of EFL business management students at the community college in Bisha, King Khalid University, Saudi Arabia. The study used a pre-test/post-test experimental and control group design. An experimental group and a control group were exposed to pre-post means of getting data (a pre-post test of persuasive writing). Results of the analysis of the differences between means of scores of the study subjects in the pre-post-measurements revealed a significant improvement in the experimental group students' persuasive writing.
A Novel Spectrophotometric Method for Determination of Five 1,4-Dihydropyridine Drugs in Their Tablets and Capsules Using Vanillin Reagent  [PDF]
Mohamed A. El Hamd, Sayed M. Derayea, Osama Hassan Abdelmageed, Hassan F. Askal
American Journal of Analytical Chemistry (AJAC) , 2013, DOI: 10.4236/ajac.2013.43020
Abstract:
A selective and new spectrophotometric method is described for determination of five 1,4 dihydropyridine drugs (1,4- DHP); namely nifedipine (NIF), nicardipine (NIC), nimodipine (NIM), felodipine (FEL) and amlodipine (AML). The method is based on a coupling reaction between the cited drugs and vanillin reagent in acidic condition. Under optimized conditions, the red coloured products were measured at 500 nm for NIF, NIC, NIM and FEL or at 479 nm for AML. Molar absorptivities were ranged from 0.575 × 104 1.065 × 104 l·mol-1·cm-1, Beers law was obeyed at 5 - 70 μg/mL concentration range and the limit of detection was ranged from 0.150 - 1.500 μg/mL. The proposed method was successfully extended to pharmaceutical preparations tablets and capsules and comparison by Student’s t-test and variance ratio F-test showed no significant difference.
Studies on the Immunomodulatory Effects of Lactoferrin in Rats Infected with E. coli  [PDF]
Mohamed El-Sayed El-Boshy, Osama Ali Abdalla, Adal Hassan
Journal of Immune Based Therapies, Vaccines and Antimicrobials (JIBTVA) , 2013, DOI: 10.4236/jibtva.2013.24005
Abstract:

Eighty male albino rats of Westar strain (350 ± 10 g), 10 to 12 weeks old were divided into four groups. The groups treated are as following. The first control group (Gp. I) was given intraperitoneally in normal saline (1 mL). The second group (Gp. II) was orally infected with 3 × 1012 CFU of E. coli /Kg. BW. The third group (Gp. III) was infected with

The Role of CK20, p53 and p63 in Differentiation of Some Urothelial Lesions of Urinary Bladder, Immunohistochemical Study  [PDF]
Sayed Abdel Raheem, Abdel Naby Saied, Rabee Al Shaer, Osama Mustafa, Ali Hassan Ali
Open Journal of Pathology (OJPathology) , 2014, DOI: 10.4236/ojpathology.2014.44024
Abstract: Background: Differentiation of urothelial hyperplasia, dysplasia and carcinoma in situ (CIS) may pose diagnostic difficulties. We aim to evaluate the role of CK20, p53 and p63 in differentiation of such lesions. Methods: we evaluate these markers in 213 cases of bladder lesions (14 hyperplasia, 7 dysplasia, 5 CIS, 25 noninvasive and 162 invasive urothelial carcinoma) in a retrospective study on sections from formalin-fixed, paraffin-embedded blocks. Cytoplasmic staining considered for CK20 and nuclear staining for p53 and p63. Results: CK20 was expressed in 14 out of 14 hyperplasia (4 strong, 7 moderate, 3 weak), in 7 out of 7 dysplasia (4 strong , 3 moderate), in 5 out of 5 CIS, in 19 out of 25 noninvasive carcinoma; strong in 11 (7 low grade, 4 high grade), moderate in 7 (5 low grade, 2 high grade), and weak in 1 case of low grade, in 136 out of 162 invasive carcinoma; strong in 46 (22 grade II, 24 grade III), moderate in 71 (2 grade I, 58 grade II, 11 grade III), and weak in 19 (2 grade I, 15 grade II, 2 grade III), no expression in 2 cases of grade IV. p53 was expressed in 7 out of 14 hyperplasia (weak in all cases), in 7 out of 7 dysplasia (5 strong, 2 moderate), in 5 out of 5 CIS (strong in all cases), in 20 out of 25 noninvasive carcinoma (8 strong, 9 moderate, 3 weak), in 135 out of 162 invasive carcinoma (34 strong, 73 moderate, 28 weak). p63 was expressed in 14 out of 14 hyperplasia, in 7 out of 7 dysplasia, in 4 out of 5 CIS, in 25 out of 25 noninvasive carcinoma, and in 134 out of 162 invasive carcinoma (28 strong, 63 moderate, 43 weak). CK20 was insignificant in noninvasive and significant in invasive carcinoma, while p53 and p63 were significant in noninvasive and invasive carcinoma. Conclusion: CK20, p53 and p63 are useful in differentiation of different bladder lesions.
Development and Validation of HPLC Method for Simultaneous Determination of Amlodipine, Valsartan, Hydrochlorothiazide in Dosage Form and Spiked Human Plasma  [PDF]
Samya M. El-Gizawy, Osama H. Abdelmageed, Mahmoud A. Omar, Sayed M. Deryea, Ahmed M. Abdel-Megied
American Journal of Analytical Chemistry (AJAC) , 2012, DOI: 10.4236/ajac.2012.36055
Abstract: A simple, sensitive, and specific method was developed for simultaneous determination of Amlodipine besylate (AML), Valsartan (Vals) and Hydrochlorothiazide (HCT) by high performance liquid chromatography without previous separation. Satisfactory resolution was achieved using a RP-C18 chromatographic column, Phenomenex Kinetex (150 mm × 4.6 mm i.d) and a mobile phase consisting of acetonitrile-phosphate buffer (0.05 M) with pH 2.8 in the proportion of (40/60, v/v) at a flow rate 0.8 mL/min and the wavelength detection was 227 nm. The retention time for HCT, AML and VAls was 2.26, 3.16 and 11.19 min; respectively. The described method was linear over a range of 4-28 μg /ml, 5-40 μg /ml and 1-12 μg /ml for AML, Vals and HCT; respectively. The mean percent recoveries were 99.94%, 99.96% and 99.78% for AML, Vals and HCT; respectively. F-test and t-test at 95%con?dence level were used to check the intermediate precision data obtained under different experimental setups. The method could be used for analysis of combined dose tablet formulation containing AML, Vals, HCT as well as spiked human plasma.
Hind-foot correction and stabilization by pins in plaster after surgical release of talipes equino varus feet in older children
Mohamed M El-Sayed, Osama A Seleem
Journal of Orthopaedic Surgery and Research , 2010, DOI: 10.1186/1749-799x-5-42
Abstract: CTEV is a complex deformity that has a tendency to recur until the age of six or seven years [1]. Recently after the introduction of the Ponseti method, there is almost a universal agreement on the non-operative management of CTEV [2-6].It is likely that a small number of clubfeet will require surgery even after expertly applied non-operative treatment. In some patients, either failure to obtain a complete correction or failure to maintain the correction occurs [6].In those patients with severe relapsed deformities, the calcaneus is often small and difficult to control during casting. A residual varus mal-positioning of the hind-foot may occur, after complete adequate surgical release. We used three pins to control and maintain the hind foot correction in the normal position (about 5° valgus) during casting in the studied 59 feet.Between Oct. 2003 and Sept. 2009, 47 cases (59 feet) of CTEV, were operated upon using the below described surgical technique. The parents gave the informed consent to include their kids into the study. There were 35 unilateral cases and 12 bilateral cases. The duration of previous conservative management ranged from 5 to 22 months, with a mean of 12 months.In all cases, a trial of conservative management, using the Ponseti method, was strongly suggested at our center which was not accepted by the parents of all the children included in this study. History of previous treatment is summarized in (Table 1) and demonstrates a history of good initial results and deformity correction following previous conservative management that was reported In 16 unilateral, and 5 bilateral patients using the Ponseti method. This was not maintained and the deformity recurred in this group of patients, and that was attributed to the poor family compliance, inadequate orthosis, and/or follow-up. History of previous surgical intervention was reported in 19 unilateral and 7 bilateral cases (33 feet), and the deformity recurred despite reported initial post-operat
Building a health care data warehouse for cancer diseases
Osama El-Sayed Sheta,Ahmed Nour Eldeen
Computer Science , 2012, DOI: 10.5121/ijdms.2012.4503
Abstract: This paper presents architecture for health care data warehouse specific to cancer diseases which could be used by executive managers, doctors, physicians and other health professionals to support the healthcare process. The data today existing in multi-sources with different formats makes it necessary to have some techniques for data integration. Executive managers need access to Information so that decision makers can react in real time to changing needs. Information is one of the most factors to an organization success that executive managers or physicians would need to base their decisions on, during decision making. A health care data warehouse is therefore necessary to integrate the different data sources into a central data repository and analysis this data.
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