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Search Results: 1 - 10 of 2355 matches for " Oocyte retrieval "
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Hemoperitoneum presenting with the use of a topical hemostatic agent in oocyte retrieval: a case report
Chatrian Amélie,Vidal Clémentine,Equy Véronique,Hoffmann Pascale
Journal of Medical Case Reports , 2012, DOI: 10.1186/1752-1947-6-395
Abstract: Introduction Hemoperitoneum may occur from an ovarian puncture point after oocyte retrieval. Case presentation We report a case of massive hemoperitoneum following transvaginal ultrasound-guided oocyte retrieval in a 33-year-old Caucasian woman. The bleeding required emergency laparoscopy because of active bleeding from the ovarian puncture point. Hemostasis was very difficult to achieve, and traditional operative procedures were not efficient. The only way to stop the bleeding was by using an absorbable fibrinogen and thrombin sealant sponge, which was applied around the ovary. During laparoscopy three pints of packed red blood were administered. No specific alteration of screening coagulation tests was found one month later. Conclusions Hemostasis can be very difficult to achieve with traditional operative procedures. Topical hemostatic agents can be useful to preserve the ovary wherever possible.
The Clinic Analysis of Diclofenac Suppository for Oocyte Retrieval Analgesia in IVF-ET Cycles  [PDF]
Yubin Li, Qingyun Mai, Tao Li, Yiping Zhong, Canquan Zhou
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.48062

Objective: To study the effect of diclofenac suppository in oocyte retrieval of IVF-ET. Study Design: 1176 patients with informed consents were enrolled into this prospective randomized controlled study. The setting was an IVF-ET program at the First Affiliated Hospital of Sun Yat-sen University, Guangzhou, China. According to the analgesic drug use, the patients were randomly divided into pethidine group (573 cases) and diclofenac suppository group (603 cases). The data of vital signs, common adverse reactions, severe adverse events and pain degree in oocyte retrieval were collected. The IVF-ET outcomes were also compared. Results: The post-operation pressure and pulse were lower in pethidine group than in diclofenac suppository group (both P<0.001).The rest vital signs were not statistically different (all P>0.05). Common adverse reactions in diclofenac suppository group were relative less (all P<0.05). Pain degree between the two groups was not statistically different (P=0.304). IVF-ET outcomes were also not statistically different (all P>0.05). There were 3 cases serious abdominal bleeding with shock in the diclofenac suppository group. Conclusion: Using diclofenac suppository in

A comparison of three different target-controlled remifentanil infusion rates during target-controlled propofol infusion for oocyte retrieval
Coskun, Demet;Gunaydin, Berrin;Tas, Ayca;Inan, Gozde;Celebi, Hulya;Kaya, Kadir;
Clinics , 2011, DOI: 10.1590/S1807-59322011000500017
Abstract: objective: to evaluate the effects of three different target-controlled remifentanil infusion rates during target-controlled propofol infusion on hemodynamic parameters, pain, sedation, and recovery score during oocyte retrieval. methods: sixty-nine women were scheduled for oocyte retrieval. target-controlled propofol infusion at an effectsite concentration of 1.5 μg/ml was instituted. the patients were randomly allocated to receive remifentanil at an effect-site concentration of either 1.5 (group i, n = 23), 2 (group ii, n = 23) or 2.5 ng/ml (group iii, n = 23). hemodynamic variables, sedation, pain, the aldrete recovery score, and side effects were recorded. results: hemodynamic variables, sedation and pain scores and the number of patients with the maximum aldrete recovery score 10 min after the procedure were comparable among the groups. the number of patients in group iii with the maximum aldrete recovery score 5 min after the procedure was significantly lower than that in groups i and ii. one patient in group ii and one patient in group iii suffered from nausea. conclusion: similar pain-free conscious sedation conditions without significant changes in hemodynamic parameters were provided by all three protocols. however, target controlled infusion of remifentanil at 1.5 or 2 ng/ml proved superior at providing early recovery compared to 2.5 ng/ml.
The Developmental Competence of Oocytes Retrieved from The Leading Follicle in Controlled Ovarian Stimulated Cycles
Daniela Paes de Almeida Ferreira Braga,Tatiana Carvalho de Souza Bonetti,Ismael Dale Cotrim Guerreiro da Silva,Amanda Souza Setti
International Journal of Fertility & Sterility , 2013,
Abstract: Background: This study compares the developmental capacity of gametes retrieved from thelargest follicle with small follicles of a cohort in controlled ovarian stimulated cycles.Materials and Methods: This prospective study performed in a private assisted fertilizationcenter included 1016 follicles collected from 96 patients who underwent intra cytoplasmic sperminjection (ICSI). After follicular aspiration, oocytes were assigned to two groups accordingto the diameter of the derived follicle. The large follicle group (n=96) comprised oocytesderived from the leading follicle of the cohort and the small follicle group (n=920) consistedoocytes derived from the smaller follicles of the cohort. The fertilization and percentage of topqualityembryos were compared between groups by Chi-square or Fisher’s exact test, whereappropriate. The effect of the follicular diameter on oocyte dimorphism was assessed by binarylogistic regression.Results: A significantly higher percentage of oocytes derived from the leading follicle were inthe metaphase II (MII) stage (100 vs. 70.0%, p<0.001). However we observed no significantdifferences regarding the percentage of degenerated oocytes between the large (6.25%) and smallfollicle (5.0%) groups (p=0.550). Regression analysis demonstrated a nearly two-fold increase in theincidence of vacuoles in oocytes derived from the largest follicle of the cohort (OR: 1.81, p=0.046).The fertilization rate (50.0 vs. 38.8%, p=0.038) and the percentage of top quality embryos (84.7 vs.76.4%, p=0.040) were significantly higher for oocytes derived from the largest follicle. However,the percentage of abnormal fertilized oocytes was equally distributed between the large follicle(15.0%) and small follicle (12. 8%) groups (p=0.550).Conclusion: Our data suggest that intrafollicular mechanisms within the larger follicle of the cohortmay allow for these follicles to amplify the responsiveness to exogenous gonadotropin, which leads tothe formation of more competent oocytes with higher fertilization and developmental capacities.
Empty follicle syndrome-Still an enigma
Krishna Deepika,Rajashekar Lavanya,Patil Madhuri
Journal of Human Reproductive Sciences , 2008,
Abstract: Empty follicle syndrome (EFS), although rare with an incidence of 0.2-7%, is a frustrating condition where no oocytes are retrieved in in vitro fertilization (IVF), even though ultrasound and estradiol measurements show the presence of many potential follicles. It is a complex phenomenon that cannot be explained by low bioavailability of human chorionic gonadotrophin alone; neither can it be reliably diagnosed by the measurement of serum beta-human chorionic gonadotrophin (bhCG) on the day of oocyte retrieval (OR), except possibly when the bhCG concentration is very low. Here we report a case who underwent intracytoplasmic sperm injection (ICSI) for her partner′s severe oligoasthenozoospermia. Controlled ovarian hyperstimulation (COH) was done in her first cycle of ICSI, using a gonadotrophin-releasing hormone (GnRH) agonist long protocol with follicle-stimulating hormone (FSH) and human menopausal gonadotrophin (HMG). However, as we were unable to retrieve any oocytes, her COH protocol was changed in the subsequent cycle with a successful outcome.
Effect of Oocyte Recovery Techniques on in Vitro Production of Swine Embryos  [PDF]
Mariana Groke Marques, Flavia Regina Oliveira de Barros, Marcelo Demarchi Goissis, Mariana Ianello Giassetti, Mayra Elena Ortiz D’Avila Assump??o, José Antonio Visintin
Open Journal of Animal Sciences (OJAS) , 2015, DOI: 10.4236/ojas.2015.54048
Abstract: In Vitro production of swine embryos is a valuable tool to generate clones and genetically modified pigs during a short period of time. However, the efficiency of the existing methods is extremely low and the oocyte quality and quantity represent important obstacles on the success of in Vitro production of embryos. Therefore, the aim of this study was to compare the in Vitro maturation, fertilization and subsequent embryo development rates of oocytes recovered by ovary slicing or follicular aspiration. The oocyte recovery rate (grade 1 COC/ovary) was higher (p = 0.0083) in the slicing group when compared to the aspiration group. No differences were observed between groups regarding in Vitro maturation and early cleavage rates. A higher percentage of oocytes recovered by follicular aspiration reached the blastocyst stage after IVF when compared to the ovary slicing method (p = 0.0395). However, no difference on blastocyst cell number was observed. Although the recovery of oocytes using the slicing technique yielded more grade 1 oocytes per ovary than the aspiration method, the number of oocytes that reached the blastocyst stage after IVF by the slicing method was lower when compared with oocytes obtained by aspiration, as observed by lower blastocyst rates. In conclusion, the follicular aspiration is the method of choice for porcine in Vitro embryo production.
The Conditions of Ovary Storage Affect the Quality of Porcine Oocytes  [PDF]
Matías Nicolás Tellado, Gabriel Martín Alvarez, Gabriel Carlos Dalvit, Pablo Daniel Cetica
Advances in Reproductive Sciences (ARSci) , 2014, DOI: 10.4236/arsci.2014.23007
Abstract: We studied the effect of different storage conditions of porcine ovaries (time, temperature) on the characteristics of the follicular fluid, immature oocyte quality, meiotic competence and in vitro fertilization of oocytes. Ovaries were stored for 2, 4 or 6 h at 3 different temperatures (15°C, 25°C or 35°C). As the storage time increased, pH and glucose concentration of the follicular fluid, percentage of live immature oocytes at germinal vesicle stage, oxidative activity and maturation rate decreased. At higher temperatures, pH and glucose concentration decreased, but oxidative activity and oocyte maturation rate increased. Lactate concentration and immature oocyte ROS production increased as storage time and temperature increased. The ovary storage for longer than 2 h at 25°C and 35°C resulted in low pH of the follicular fluid and high ROS level in immature oocytes. Such conditions seem to damage oocytes and impair their meiotic competence. A decrease in the oxidative activity caused by long time and/or low storage temperature may imply a decrease in oocyte vitality. In conclusion, in the porcine species, the transport of ovaries at 25°C and 35°C for 2 h are the best conditions to maintain adequate oocyte quality, meiotic competence and in vitro fertilization rates.
Elective oocyte freezing for the preservation of fertility  [PDF]
Jason Barritt, Martha Luna, Benjamin Sandler, Marlena Duke, Alan B. Copperman
Open Journal of Obstetrics and Gynecology (OJOG) , 2012, DOI: 10.4236/ojog.2012.21005
Abstract: Oocyte cryopreservation has recently emerged as an option for women to preserve their fertility for medical (e.g. treatable malignancy) or elective indications (e.g. advancing age). This report describes an IRB-approved study of over 200 oocyte cryopreservation cycles at one center. Patients presenting for oocyte cryopreservation (January 2005 to 2010) were analyzed for day 3 follicle stimulating hormone (FSH), basal antral follicle count (BAFC), gonadotropin usage and the number of oocytes retrieved and cryopreserved. New patient consultations were performed on 516 women, of whom 175 (34%) proceeded to initiate a total of 233 cryopreservation cycles. Twenty-four cycles were cancelled (10%) after starting follicular stimulation due to poor ovarian response or self-withdrawal of the patients. Patients whose cycles were cancelled demonstrated a higher Day 3 FSH and a lower BAFC than patients who completed cycles (p < 0.01). In the 209 completed cycles, the most important predictors of a successful cycle included BAFC (r = 0.36), FSH (r = –0.25) and age (r = –0.18) with the mean number of oocytes cryopreserved at 13.6 ± 8.8. Information about long-term fertility preservation must reach both patients and health care providers so that more women can be educated about the benefits of proactive early physiological reproductive assessment and possible interventions available.
Oocyte quality improvement using a herbal medicine comprising 7 crude drugs  [PDF]
Taketo Inoue, Yoshiyuki Ono, Yukiko Yonezawa, Michinobu Oi, Naomi Kobayashi, Junji Kishi, Nobuyuki Emi
Open Journal of Obstetrics and Gynecology (OJOG) , 2013, DOI: 10.4236/ojog.2013.31A036

Maternal age is a significant factor in infertility treatment. Ovarian function and oocyte quality decrease with age, whereas the frequency of chromosomal abnormalities increases. In this study, improvement of oocyte quality and ovarian function were attempted using a herbal medicine comprising 7 crude drugs:Angelicae radix,Rehmanniae radix,Plantaginis semen,Lonicerae flos,Carthami flos,Ginseng radix, andCucurbita moschata Duch. Thirty-one women who repeatedly failed to conceive by intracytoplasmic sperm injection took the herbal medicine before breakfast and dinner from the start of menstrual cycle in the ovum pickup cycle. Average patient age was 38.5 ± 0.7 years, and the average ovum pickup frequency on the first dosage day was 7.9 ± 1.5. To analyze the effects of herbal medicine intake, the number of recovered and mature oocytes, their morphology and physical qualities, as well as the rates of fertilization, oocyte development, and pregnancy was compared before and after intake. The recovered and mature oocyte numbers, oocyte morphology and physical qualities, and fertilization rate were not significantly different before and after drug intake. However, the oocyte development rate was significantly higher(58.0%) after herbal medicine intake than before (32.5%; p = 0.0003). Moreover, the successful pregnancy rate was significantly higher after intake than before (6.9% versus 0%; p = 0.0111). Herbal medicine may constitute a useful adjunct to assisted reproductive technology in women.

0ocyte Related Factors and Chance of Implantation
Tahereh Madani,Mahnaz Ashrafi,Kiandokht Kiani,Khafri Soraya
International Journal of Fertility & Sterility , 2007,
Abstract: Background: In IVF/ICSI treatment, several attempts have been made to quantify the implantation potential of embryos in order to optimize the pregnancy rate. The objective was to determine the possible factors which might have positive impact on implantation.Materials and Methods: We retrospectively analyzed 110 IVF- ICSI cycles as first trial for ART programs. Maternal and ovulation factors such as female age, body mass index, type of infertility, infertility diagnosis, duration of ovulation stimulation, numbers of aspirated and fertilized oocytes, day of ET, were compared in high (≥2 gestational sacs) and low implantation groups (one or no sac). All analyses were adjusted for age and PCO subgroups in line with the design of the study.Results: Our results showed that there were significant differences in follicle size between high and low implantation groups (19.32±0.37 vs. 18.07±0.32 respectively) (p= 0.014). Mean number of grade (V) oocytes was also statistically greater in high implantation group (p=0.035). Our results were also compared based on age and PCO diagnosis. Women younger than 35 years old in high implantation group had higher number of grade (V) oocytes than the other group (p=0.038). Assessing our results based on PCO diagnosis, we found that the number of oocytes grade (IV) were significantly higher in high implantation group with non PCOs diagnosis (4.82±2.87 vs. 4.25±3.6) (p=0.043). Non PCO women in low implantation group had also greater number of grade II oocytes compared to the other group (p=0.017). The mean follicle size was significantly greater in high implantation group than the other group (19.32±2.17 versus 18.07±1.76) (p=0.014).Conclusion: Follicle size, and oocyte quality have positive effect on high implantation potential.
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