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Search Results: 1 - 10 of 190233 matches for " Odile G. Nacoulma "
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Phytochemical Composition, Antioxidant and Xanthine Oxidase Inhibitory Activities of Amaranthus cruentus L. and Amaranthus hybridus L. Extracts
Fernand W. Nana,Adama Hilou,Jeanne F. Millogo,Odile G. Nacoulma
Pharmaceuticals , 2012, DOI: 10.3390/ph5060613
Abstract: This paper describes a preliminary assessment of the nutraceutical value of Amaranthus cruentus (A. cruentus) and Amaranthus hybridus (A. hybridus), two food plant species found in Burkina Faso. Hydroacetonic (HAE), methanolic (ME), and aqueous extracts (AE) from the aerial parts were screened for in vitro antioxidant and xanthine oxidase inhibitory activities. Phytochemical analyses revealed the presence of polyphenols, tannins, flavonoids, steroids, terpenoids, saponins and betalains. Hydroacetonic extracts have shown the most diversity for secondary metabolites. The TLC analyses of flavonoids from HAE extracts showed the presence of rutin and other unidentified compounds. The phenolic compound contents of the HAE, ME and AE extracts were determined using the Folin–Ciocalteu method and ranged from 7.55 to 10.18 mg Gallic acid equivalent GAE/100 mg. Tannins, flavonoids, and flavonols ranged from 2.83 to 10.17 mg tannic acid equivalent (TAE)/100 mg, 0.37 to 7.06 mg quercetin equivalent (QE) /100 mg, and 0.09 to 1.31 mg QE/100 mg, respectively. The betacyanin contents were 40.42 and 6.35 mg Amaranthin Equivalent/100 g aerial parts (dry weight) in A. cruentus and A. hybridus, respectively. Free-radical scavenging activity expressed as IC 50 (DPPH method) and iron reducing power (FRAP method) ranged from 56 to 423 μg/mL and from 2.26 to 2.56 mmol AAE/g, respectively. Xanthine oxidase inhibitory activities of extracts of A. cruentus and A. hybridus were 3.18% and 38.22%, respectively. The A. hybridus extract showed the best antioxidant and xanthine oxidase inhibition activities. The results indicated that the phytochemical contents of the two species justify their traditional uses as nutraceutical food plants.
Prevalence of blaSHV genes in clinical isolates of Klebsiella pneumoniae at Saint Camille medical Center in Ouagadougou. Isolation of blaSHV11-like gene
Boukaré Zeba, Pere Jacques Simporé, Odile G Nacoulma, Jean-Marie Frere
African Journal of Biotechnology , 2004,
Abstract: Five bacterial strains (4 Klebsiella pneumoniae and 1 Escherichia coli) representative of pathogenic species and resistant to β-lactam antibiotics are investigated to isolate the genes responsible of β--lactamase activity. The use of engineering techniques enables us to show the widespread of blaSHV genes particularly in clinical isolates of K. pneumoniae. Our results highlighted an atypical blaSHV-11 gene.
Polyphenol Content and Antioxidant Activity of Fourteen Wild Edible Fruits from Burkina Faso
Aline Lamien-Meda,Charles Euloge Lamien,Moussa M.Y. Compaoré,Roland N.T. Meda,Martin Kiendrebeogo,Boukare Zeba,Jeanne F. Millogo,Odile G. Nacoulma
Molecules , 2008, DOI: 10.3390/molecules13030581
Abstract: A total of fourteen (14) species of wild edible fruits from Burkina Faso wereanalyzed for their phenolic and flavonoid contents, and their antioxidant activities usingthe DPPH, FRAP and ABTS methods. The data obtained show that the total phenolic andtotal flavonoid levels were significantly higher in the acetone than in the methanol extracts.Detarium microcarpum fruit had the highest phenolic and the highest flavonoid content,followed by that of Adansonia digitata, Ziziphus mauritiana, Ximenia americana andLannea microcarpa. Significant amounts of total phenolics were also detected in the otherfruit species in the following order of decreasing levels: Tamarindus indica > Sclerocaryabirrea > Dialium guineense > Gardenia erubescens > Diospyros mespiliformis > Parkiabiglobosa > Ficus sycomorus > Vitellaria paradoxa. Detarium microcarpum fruit alsoshowed the highest antioxidant activity using the three antioxidant assays. Fruits with highantioxidant activities were also found to possess high phenolic and flavonoid contents.There was a strong correlation between total phenolic and flavonoid levels and antioxidantactivities.
Antiacetylcholinesterase and antioxidant activity of essential oils from six medicinal plants from Burkina Faso
Kiendrebeogo, Martin;Coulibaly, Ahmed Y.;Nebie, Roger C. H.;Zeba, Boukaré;Lamien, Charles E.;Lamien-Meda, Aline;Nacoulma, Odile G.;
Revista Brasileira de Farmacognosia , 2011, DOI: 10.1590/S0102-695X2011005000008
Abstract: in this investigation, we evaluated essential oils from six medicinal plants from burkina faso for their antiacetylcholinesterase and antioxidant abilities. the chemotype of most active were also determined. the best antiacetylcholinesterase activities were recorded for the essential oils of eucalyptus camaldulensis (ic50 18.98 μ g/ml) and ocimum canum (ic50 36.16 μ g/ml). their chemotype have been related to the 1,8-cineole one. both essential oils demonstrated a linear mixed non competitive inhibition. the essential oil of ocimum basilicum which belong to the linalool-eugenol chemotype exhibited the best radical scavenging activity (ic50 3.82 μ g/ml) and reducing power (531.75 mg aae/g). in comparison with gallic and ascorbic acids, o. basilicum essential oil evidenced interesting antioxidant activities. the antiacetylcholinesterase and antioxidant activities of essential oils were discussed in regard with their chemical composition.
β-lactamase Molecular Classes Linked to Production Ways from Clinical Isolates in Burkina Faso/West Africa
Boukare Zeba,Jacques Simpore,Odile Germaine Nacoulma
Journal of Applied Sciences , 2007,
Abstract: β-lactamase production ways and inhibition patterns were investigated in cell suspensions of clinical isolates. The purpose of this research was prior to investigate the different β-lactamase molecular classes occurring in Burkina Faso owing to the local practice of β-lactam antibiotics. The use of specific inhibitors enabled to draw up an inhibition profile and consequently to assign an enzyme to accurate molecular class of β-lactamase. At the same time, β-lactamase expression ways were explored and correlated to the inhibition profiles. The current results pointed out two main groups of organisms. The first group of isolates secretes β-lactamases mainly by inducible way. Interestingly this bacterial group carries molecular class C of β-lactamase. The second group of isolates uses partial inducible and partial constitutive way for enzyme expression. These bacteria mostly carry molecular class A of β-lactamases. The sole exception encountered during these investigations is an organism, expressing exclusively by the constitutive way an enzyme that is found to belong to molecular class B of β-lactamases.
Biological and Toxicological Study of Aqueous Root Extract from Mitragyna inermis (Willd oktze) Rubiaceae
Yamba Ouedraogo,Innocent Pierre Guissou,Odile Germaine Nacoulma
International Journal of Pharmacology , 2007,
Abstract: We reported the results of biological and toxicological study, realized on Mitragyna inermis Willd Oktze, one specie of family of Rubiaceae, well known in traditional medicine in Burkina Faso for his intensification potentialities of resistance against multiple pathologies like infectious and parasitic diseases, adynamia, rheumatic and osteoarthritis diseases. So we have formulated hypothesis of stimulation of organism’s defenses for a scientist research. The steeping freeze-dried of the plant’s product has been used for different assays: General acute toxicity estimation on Ico mouse (NMRI Han) by intraperitoneal route and orally administration on rabbit from the value of LD50 obtained with the mouse. Biological study: The kinetic interaction between the plant’s product chemical group and the evolution of biological elements medium of immunity on the rabbit, has been appreciated. The biological elements include white blood cells, red blood cells, lymphocytes, platelets, total proteins, albumin and globulins. The following results have been obtained about the study: A Lethal Dose (LD50) resulted from maceration (acute general toxicity) at the rate of 800 mg kg-1 of corporal weight showing a bit toxic product. An interaction between vegetable’s extract chemical group and biological elements of rabbit which is expressed by: An increasing (13 to 18%) of total proteins from serum; this increasing was notably after 24 h of administration. Albumin decreasing of 10% in comparison with initial rate, indicated haptens action of plant’s extract chemical products. α1, α2, β and γ globulins increasing, respectively 46.8, 14.31 and 26% during the first day of administration of the extract. A lymphocyte increasing of 35% 24 h after administration of the product. This rate is more increased after the second administration; White blood cells are also increasing. These results show an obvious capacity of the plant’s macered extract to stimulate organism natural defenses in relation with antigenous-antibody reaction. This will be an interesting perspective for complementary treatment of pathology like HIV diseases.
Antimicrobial activity of polyphenol-rich fractions from Sida alba L. (Malvaceae) against co-trimoxazol-resistant bacteria strains
Kiessoun Konaté, Adama Hilou, Jacques Mavoungou, Alexis Lepengué, Alain Souza, Nicolas Barro, Jacques Y Datté, Bertrand M'Batchi, Odile Nacoulma
Annals of Clinical Microbiology and Antimicrobials , 2012, DOI: 10.1186/1476-0711-11-5
Abstract: In vitro antibacterial (MIC, MBC and time-kill studies) of polyphenol-rich fractions from Sida alba L. (Malvaceae) was assessed using ten bacteria strains (Gram-negative and Gram-positive).All test bacteria were susceptible to the polyphenol-rich fractions. Time-kill results showed that after 5 h exposition there was no viable microorganism in the initial inoculum and the effect of polyphenol-rich fractions was faster on Enterococcus faecalis (Gram-positive bacterium) comparatively to the other bacteria strains.The data analysis indicates that the tested of polyphenol-rich fractions has significant effects when compared with the standard antibiotic. These results therefore justify the traditional use of sida alba L., alone or in combination with other herbs to treat bacterial infections.The use of medicinal herbs in traditional system of medicine is a common practice in many cultures around the world especially in African societies. This practice has gained widespread acceptance in developing as well as in developed nations. Researchers are also beginning to appreciate the role of medicinal plants in health care delivery [1]. In recent time, interest with herbal medicine for antimicrobial activities has been increased significantly. This is as a result of the effectiveness, low cost and the availability of these herbal medicines, the economic crisis, high cost of industrialized medicines, inefficient public access to medical and pharmaceutical care, in addition to the side effects caused by synthetic drugs are some of the factors contributing to the central role of medicinal plants in health care [2,3].So, there is serious need to develop new antimicrobial agents that are very effective with minimal unwanted side effects and higher plants represent a potential source of novel antibiotic prototypes [4]. Medical plants have shown a promising alternative for the treatment of infectious diseases. In the antibacterial research, the vast majority, 78% of the new chemical
Toxicological Assessment of Methanolic Stem Bark and Leaf Extracts of Entada africana Guill. and Perr., Mimosaceae
A. Tibiri,J.T. Banzouzi,A. Traore,G.O Nacoulma
International Journal of Pharmacology , 2007,
Abstract: This study was aimed to assess the possible toxic effects of Entada africana, a widely used African medicinal plant. The acute toxicity of the methanolic stem bark and leaf extracts of Entada africana Guill. and Perr., (Mimosaceae) was assessed on mice. It revealed an average toxicity with a LD50 of 146.7 and 249.9 mg kg-1 body weight for stem barks and leaves, respectively. The extracts showed no cytotoxicity against KB and Vero cells. Sub-chronic toxicity was assessed in rabbits, which received orally, daily for a month, a dose corresponding to 10% of the LD50. Compared to the control group this dose caused no significant (p>0.05) modification of haematological and biochemical parameters, total cholesterol, urea, creatinine and aspartate amino-transferase (AST). The extracts lowered serum glucose significantly (p<0.05) by 52% at first two weeks of treatment. The stem bark and leaf extracts showed temporary decrease (p<0.05) of Alanine amino transferase (ALT) by 26.1 and 39.1%, respectively. The stem bark extracts increased triglycerides significantly (p<0.01) by 108% at the end of last week of treatment. These investigations seemed to indicate the safety ob sub-chronic oral administration (up to 14.67 and 24.9 mg kg-1 body weight) of the methanolic extracts of Entada africana in rabbits.
Content of Polyphenolics Constituents and the Antioxidant and Antimicrobial Activities of Extracts from Leaves and Fruits of Lannea microcarpa Engl. & K. Kraus (Anacardiaceae)
J.H. Bationo,A. Hilou,P.W. Savadogo,O.G. Nacoulma
Current Research Journal of Biological Sciences , 2012,
Abstract: Nowadays, a number of studies on health benefits associated with natural compounds have been demonstrated. Polyphenolics in fruits, leaves, herbs and vegetables possess potent antibacterial and antioxidant activities. In the present study, a portion of Lannea microcarpa’s lyophilised fruits and leaves aqueous acetone extract, was fractionated by successive liquid-liquid partitioning to obtain ethyl acetate and n-butanol fraction. The total polyphenolic constituents and total flavonoid constituents from the extract and the two fractions were determined and were found to be the highest respectively in leaves butanol fraction (22.05±0.78 mg GAE/100 mg) for polyphenolic constituents, and in leaves and fruits ethyl acetate fraction (1.61±0.02 mg QE/100 mg) for the flavonoids constituents. Antioxidant activity of the extract and the two fractions were determined by in vitro model using DPPH; Antibacterial activity of these extracts and fractions was also assayed on two Gram+ bacteria and six Gram-bacteria by using disc diffusion and broth micro dilution method. Data indicated highest antioxidant activity obtained from leaves extracts and fractions IC50 (1.55±0.19 μg/mL), and inhibitory effect both on Gram- and Gram+ bacteria with highest antibacterial activity MIC (0.15625 mg/mL), obtained with leaves ethyl acetate and n butanol fraction on Bacillus cereus (ATCC 9144). All the results obtained from this study, showed that Lannea microcarpa’s fruit and leaves extract could be candidate for news antibacterial and antioxidant compounds.
Radical Scavenging Activity, Phenolic Content and Cytotoxicity of Bark and Leaves Extracts of Entada africana Guill. and Perr. (Mimosaceae)
A. Tibiri,O. Rakotonandrasana,G.O. Nacoulma,J.T. Banzouzi
Journal of Biological Sciences , 2007,
Abstract: Dried ground leaves and barks of Entada africana were extracted by maceration in methanol and fractioned with chloroform, ethyle acetate and water. The total phenolic content of each fraction was determined spectrophotometrically according to Folin-Ciocalteu`s method and calculated as Tannic Acid Equivalent (TAE). Tannins and flavonoids were also determined. The total phenolic content was quite high, especially in the aqueous fraction (up to 39.7% TAE in the barks and 39.9% in the leaves). The antioxidant activity of lyophilized extracts was determined at room temperature by the means of the 2,2-diphenyl-1-picrylhydrazyl (DPPH!) colorimetric method with a detection scheme at 517 nm and expressed as EC50. The radical scavenging activity was evaluated as the difference in absorbance between a test sample and the control (methanol). Bark extracts had the best EC50, similar to those of rutoside and ascorbic acid for the aqueous and methanol fraction (5.7 and 5.3 μg mLG1, respectively). All the other extracts were moderately active (EC50 ranging from 6.9 to 20.0 μg mLG1), except the chloroform extracts (EC50 > 69 μg mLG1). Except for the crude and aqueous bark extracts, no extract was cytotoxic on KB or Vero cells.
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