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Search Results: 1 - 10 of 707 matches for " Obreht Dragana "
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Identification of Glu-B1 alleles in bread wheat cultivars using PCR
Obreht Dragana,Kobiljski Borislav,?an Mihajla,Vapa Ljiljana
Genetika , 2007, DOI: 10.2298/gensr0701023o
Abstract: High dough strength is used as a predictor of good quality bread wheat and it has been attributed largely to the type of allele present at the Glu-D1 locus, where the Glu-D1d allele is most favorable. Recently, it has been observed that cultivars with over expressed subunit Bx7OE at the Glu-B1 have enhanced dough strength. In order to implement recently developed PCR assay for Glu-B1 x-type allele discrimination in marker-assisted selection, a set of wheat cultivars from 11 countries was analyzed. The PCR results matched the known Glu-B1 HMW GS genotypes of the cultivars possessing subunits Bx7 or Bx17. It was shown that this molecular marker also differentiated some other Glu-B1 alleles: Bx6, Bx13, Bx20 and Bx22. These results create the opportunity for an improved method of polymorphism scoring at the Glu-B1 in bread wheat germplasm. .
Marker assisted selection in BMQ related breeding programs
Obreht Dragana,Kobiljski Borislav,?an Mihajla,Vapa Ljiljana
Genetika , 2008, DOI: 10.2298/gensr0801039o
Abstract: Implementation of marker assisted selection (MAS) in bread making quality (BMQ) oriented breeding programs could allow genetic potential assessment of genotypes prior to their phenotypic evaluation. The mechanisms underlying some quality traits in wheat are now understood. This knowledge, coupled with the availability of the DNA sequences of the genes encoding gluten proteins and the wide application of the PCR, has enabled the design of diagnostic DNA markers for these quality traits. Bread wheat breeding programs developed in Institute of Field and Vegetable Crops, Novi Sad have originated a wide range of quality cultivars with strong flours and hard grain texture. During twenty years, in the process of bread-making quality prediction, composition of HMW glutenin subunits were analyzed beside standard technological parameters. However, in order to improve our breeding strategies new generations of PCR-based BMQ related markers were included in selection programs. This paper provides an overview of diagnostic DNA markers that are currently in use in foreign and domestic wheat selection programs. .
Evaluation of wheat Rht genes using molecular markers
To?ovi?-Mari? Biljana,Kobiljski Borislav,Obreht Dragana,Vapa Ljiljana
Genetika , 2008, DOI: 10.2298/gensr0801031t
Abstract: Using STSs and SSR markers, three worldwide the most important major height reducing genes, Rht-B1b, Rht-D1b and Rht8 were evaluated in this paper. In the analysed set of hexaploid wheat, composed of 172 genotypes originated from more than 20 countries, Rht-B1b, Rht- D1b and Rht8 were found in 40%, 22% and 62% of cases, respectively. In genotype groups of domestic and foreign origin, the highest difference in allele frequency was determined in the case of Rht8, which was more frequent in domestic genotypes (78, 3%). The Rht-B1b was dominantly present in foreign germplasm (57, 6%). Portion of Rht-D1b was almost equal with 22, 6% in domestic and 21, 2% in foreign varieties. Obtained results and accepted methodology for detection of these three, the most important Rht genes, represent great start point for Marker Assisted Selection (MAS) for high yielding wheat genotypes in agro climatic conditions of Serbia and Mediterranean area. .
HMW glutenin variation and rye chromatine presence in wheat genome
Obreht Dragana R.,Vapa Ljiljana B.,Davidovi? Mihajla R.
Zbornik Matice Srpske za Prirodne Nauke , 2003, DOI: 10.2298/zmspn0305043o
Abstract: For estimation of wheat end-product quality during wheat breeding programs composition of high-molecular-weight glutenin subunits (HMW GS) and the presence of 1BL/1RS translations serve as markers due to their profound effects on dough elasticity and viscous properties. Ninety-three wheat genotypes from Institute of Field and Vegetable Crops in Novi Sad have been analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in order to determine their HMW GS composition and 1BL/1RS translocation presence. Eleven alleles were found at the Glu-1 loci. Subunits 1 and 2*and the null allele N were determined at the Glu-A1 locus. Subunitis 7, 7+9, 7+8, 6+8, 20 and 21 were found at the Glu-B1 locus, subunits 2+12 and 5+10 at the Glu-D1 locus. The 1BL/1RS translocation was discovered in 28 cultivars, although three of them were heterogeneous.
An assessment of the genetic diversity in the wild boar population from the Podunavlje-Podravlje hunting area
Veli?kovi? Nevena,?an Mihajla,Zori? M.,Obreht Dragana
Archives of Biological Sciences , 2010, DOI: 10.2298/abs1003807v
Abstract: nema
Molecular characterization of glutenin alleles at the Glu-Dl locus
Vapa Ljiljana B.,Obreht Dragana R.,Kobiljski Borislav ?.,Davidovi? Mihajla R.
Zbornik Matice Srpske za Prirodne Nauke , 2003, DOI: 10.2298/zmspn0305035v
Abstract: It is well known that the composition of high-molecular-weight (HMW) glutenin subunits impacts the bread making quality. The HMW subunits 1Dx5-1Dyl0 are typically associated with high dough strength and good bread making quality contrary to 1Dx2-lDyl2 subunits. Bread wheat cultivars from Institute of Field and Vegetable Crops in Novi Sad have been screened for the alleles present at Glu-Dl locus using traditional SDS PAG electrophoresis method and a new PCR based approach. The Glu-Dl locus was screened for two main x-type alleles which code for HMW glutenins 2 and 5, and two main y-type alleles which code for HMW glutenin subunits 10 and 12. Among the analyzed cultivars 55.6% expressed the presence of 1Dx5 and 1Dy10 alleles at the Glu-Dl locus. These results confirmed that by using marker-assisted selection (MAS) it is possible to identify genotypes with alleles for good bread making quality which could be successfully used in wheat breeding programs.
Seed protein variability in safflower
Obreht Dragana R.,Vapa Ljiljana B.,Ki? Sándor A.,Takács Mária-Hajos
Zbornik Matice Srpske za Prirodne Nauke , 2002, DOI: 10.2298/zmspn0201029o
Abstract: Total seed proteins in two safflower species (Carthamus tinctorius L. and C. lanatus L) have been separated by the SDS-PAGE method. Their molecular masses ranged from 120 to 20 kDa. All C. tinctorius genotypes under study exhibited identical electrophoretic patterns which differed from the pattern exhibited by the wild species C. lanatus in the number and position of protein bands. Differences in protein profiles occurred in regions around 60 kDa, from 43 to 36 kDa and around 30 kDa. Statistically significant differences in seed protein content were found among safflower genotypes from different countries as well as among genotypes from the same country but from different sites. The highest seed protein content was found in a genotype originating from the USA.
Genetic variability of pheasant (Phasianus spp) in breeding station Ristova a
Vapa Ljiljana B.,?an Mihajla R.,Obreht Dragana R.,To?ovi?-Mari? Biljana M.
Zbornik Matice Srpske za Prirodne Nauke , 2004, DOI: 10.2298/zmspn0417005v
Abstract: One of the possible reasons for pheasant population number decline in past several years might be loss of adaptability in populations originated from breeding stations caused by inbreeding depression. Due to fact that adaptability is a consequence of genetic structure of the populations, the aim of this paper was the analysis of genetic variability in pheasant population from breeding station Ristovaca using molecular markers. Allozyme variability of 20 putative gene loci was detected by polyacrylamide gel electrophoresis. Polymorphism was revealed in 5 loci: Est-1, Pgd, Sod, Gpi-2 and Odh. The values of genetic variability measures - heterozigosity polymorphism, fixation indices and H/P ratio indicate low level of genetic variability and possible presence of inbreeding depression within pheasant population.
On gene pool divergence of the brown hare (Lepus europaeus, Pallas) in Vojvodina
?an Mihajla R.,Vapa Ljiljana B.,Obreht Dragana R.,Vapa Milan M.
Zbornik Matice Srpske za Prirodne Nauke , 2004, DOI: 10.2298/zmspn0417013d
Abstract: Today it is widely accepted that a conservation of genetic diversity increases chances of animal survival. The aim of this study was investigation of gene pool divergence of the brown hare (Lepus europaeus) in Vojvodina. Allozymic diversity of 60 brown hares from different localities in Vojvodina was studied by polyacrylamide and starch gel electrophoresis at 31 putative structural gene loci. Only five loci: Idh-2, Pgd, Pgm, Es-1 and Es-D were polymorphic, possessing 2 to 6 different alleles. The value of average heterozygosity (Ho) was 0.0427, while polymorphism (P99%) was 8.4%. Nei's values of genetic distance (ranged from 0.000 to 0.029) and modified Roger's distance (ranged from 0.030 to 0.181) were calculated among hare populations. Apart from the relatively high values of heterozygosity and polymorphism, the nuclear gene pool diversity of brown hare population in Vojvodina based on allozyme variation is low and corresponds to the data obtained for the populations in Austria and Central Europe.
Radon indoor concentrations and activity of radionuclides in building materials in Serbia
Popovi? Dragana,Todorovi? Dragana
Facta Universitatis Series : Physics, Chemistry and Technology , 2006, DOI: 10.2298/fupct0601011p
Abstract: The paper presents the results of radon indoor concentrations measurements in homes and public institutions (kindergardens) in Belgrade, as well as the results of the radionuclides content determination in building materials from Serbia, during the eighties and up to the mid-nineties of the 20th century. The activity of the radionuclides was determined on an HPGe detector (ORTEC relative efficiency 20%) by standard gamma spectrometry. Radon indoor concentrations on the grab samples of air were determined by alpha scintillation technique (Lucas scintilation cell with ZnS/Ag). Mean effective dose equivevalents of radon and its progenies in closed space were estimated at 0.074 mSv for radon and 1.22 mSv for its short-lived daughters, in total 1.3 mSv.
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