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Search Results: 1 - 10 of 18 matches for " ORAWAN CHATCHAWANKANPANICH "
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Molecular Identification and Sequence Analysis of Tobacco Leaf Curl Begomovirus from Jember, East Java, Indonesia
HAYATI Journal of Biosciences , 2008,
Abstract: Begomovirus had been proved as the causal agent of leaf curl disease in tobacco in Indonesia, or commonly in Indonesia called as penyakit krupuk tembakau. Association of Begomovirus with the disease was further confirmed by sequence analysis. Amplification of the virus was conducted following whitefly (Bemisia tabaci Genn.) transmission. Fragment of DNA 1.6 kb was amplified by polymerase chain reaction (PCR) located within the replication initiator protein gene and coat protein gene (top region). Conserved sequence of stem loop region was found, included nonanucleotide sequence TAATATTAC present in all geminiviruses. Begomovirus associated with leaf curl disease in tobacco showed the closest relationship with Ageratum yellow vein virus - Zimbabwe, a strain of Tobacco leaf curl virus from Southern Africa. It was also known that Begomovirus associated with leaf curl disease in tobacco from Jember, East Java was different from other Indonesian Begomoviruses reported earlier.
Response of resistant breeding lines of tomato germplasm and their progenies with Seedathip3 to Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2])
Ornubol Chomdej,Orawan Chatchawankanpanich,Wichai Kositratana,Julapark Chunwongse
Songklanakarin Journal of Science and Technology , 2007,
Abstract: Tomato germplasm accessions; FLA456-4, FLA591-15, H24, CLN2443A, CLN2443B, CLN2443C, TLB111, TLB182-1, TLB111-F6-4-1, TLB130-F6-3-1 and TLB134-F6-8-1 from the Asian Vegetable Research Development Center (AVRDC), Taiwan, were screened for resistance to the Tomato Yellow Leaf Curl Virus, Thailand isolate (TYLCTHV-[2]). The accessions expressing the resistant genotype were then crossed to the TYLCV-susceptible female parent, Seedathip3 (SD3), to produce F1 hybrids. Tomato parents and their F1 progenies were inoculated with TYLCTHV-[2] at 3 weeks of seedling age using viruliferous whitefly (Bemisia tabaci) as the inoculation vector. Disease response of the seedling was rated according to the incidence and severity of the development of yellowing and curling symptoms. The presence of TYLCTHV-[2] in the inoculated plants was confirmed by Enzyme-Linked Immunosorbent Assay (ELISA). AVRDC tomato parental lines: H24, FLA591-15 and FLA456-4 expressed mild or no symptoms after one month inoculation. Progeny of crosses between the AVRDC donor parental lines and susceptible Thai cultivars showed intermediate tolerance to TYLCTHV-[2] infection. This indicated that resistance was incompletely dominant.
Resistance to tomato yellow leaf curl Thailand virus,TYLCTHV-[2] from Solanum habrochaites accession L06112 in F1 and BC1F1 generations
Ornubol Chomdej1,Sukhuman Whankaew1,,Orawan Chatchawankanpanich2,,Wichai Kositratana3
Songklanakarin Journal of Science and Technology , 2008,
Abstract: Resistance to tomato yellow leaf curl disease caused by Tomato yellow leaf curl Thailand virus (TYLCTHV-[2]) inwild tomato, Solanum habrochaites L06112 was investigated. The L06112 accession expressing the resistant phenotypewas crossed to the TYLCV-susceptible female parent, Seedathip3, to produce F1 hybrids. Parental polymorphism and hybrididentity were tested using 12 pairs of microsatellite markers for each chromosome. All markers were polymorphic betweenthe parents, but only markers SSR46, SSR115, SSR117 and SSR128 gave results suitable to assess hybrid relationships.Polymorphic bands were sharp, concise and distinguishable between hybrids and selfed plants. The stem cuttings of donorand recurrent parents, their F1 and BC1F1 were inoculated with TYLCTHV-[2] using viruliferous whiteflies. Diseaseresponse of the plants was evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) at 45 days post inoculation. Thedonor parental line showed complete resistance to TYLCTHV-[2] while the F1 and BC1F1 expressed various ELISA readingsfor TYLCTHV-[2] concentration. BC1F1; 04T105-7, 04T105-1, 04T105-10, 04T109-4 and 04T104-1 developed from thisstudy showed the high level of resistance to TYLCV, Thailand isolate.
Effect of dexamethasone on differential white blood cell counts and heterophil / lymphocyte ratio in Japanese quails (Coturnix coturnix japonica)
Worapol Aengwanich,Orawan Chinrasri
Songklanakarin Journal of Science and Technology , 2003,
Abstract: Laying Japanese quails (n = 60), 30 weeks of age reared at the experimental laboratory unit of the Faculty of Technology, Maha Sarakham University, Maha Sarakham province, Thailand. Birds were in cages with wire floors. Randomized Complete Block was the design of the experiment. During the first 4 days of experimental period, quails were fed on a standard commercial diet with four treatments: supplemented with dexamethasone at 4 levels namely 0 (control group), 1.25, 2.50 and 5.00 mg/kg diet. On days 1, 3, 7, 10 and 14 of the experimental period, percentage of heterophil, lymphocyte, monocyte, basophil, eosinophil, and heterophil/ lymphocyte ratio values of laying quails were examined. The results revealed that percentage of heterophil of laying quail with added dexamethasone at 1.25 and 2.50 mg/kg in diets were significantly higher than others (P<0.05). Percentage of lymphocyte of Japanese quails receiving dexamethasone at 1.25 and 2.50 mg/kg was significantly lower than birds receiving dexamethasone at 0 and 5.00 mg/kg (P<0.05). Heterophil: lymphocyte ratio of laying birds supplemented with dexamethasone at 1.25 and 2.50 mg/kg in diets was significantly higher than that of laying quail that added dexamethasone at 0 and 5.00 mg/kg in diets (P<0.05). The percentage of monocyte, basophil and eosinophil after receiving dexamethasone each levels were not significantly different (P>0.05). After discontinuing dexamethasone administration on day 4 of the experimental period, percentage of heterophil, lymphocyte and eosinophil, monocyte, and heterophil: lymphocyte ratio, of quails recovered to normal within 10 days.
Blood Cell Characteristics, Hematological Values and Average Daily Gained Weight of Thai Indigenous, Thai Indigenous Crossbred and Broiler Chickens
Chinrasri Orawan,W. Aengwanich
Pakistan Journal of Biological Sciences , 2007,
Abstract: This investigation was carried at the Experimental Laboratory Unit, Division of Animal Production Technology, Faculty of Technology, Mahasarakham University, Mahasarakham, Thailand in August to December 2004. Three different breeds of poultry were used, i.e., Thai indigenous, Thai indigenous crossbred and broiler chickens. The experiment was laid in a split plot design with three replications. The three poultry breeds were used as main plots, whilst gender (male and female) and sampling periods were used as subplots. An assay on blood characteristics and blood counts of red and white blood cells were carried out. Feed intake and average daily gained weight (ADG)/week were determined. The results showed that the appearances on blood cells characteristics of erythrocyte of red blood cells and white blood cells of heterophil, eosinophil, monocyte, basophil and thrombocyte of the three poultry breeds were not different from one another. Hematological values of the three different breeds possessed normal blood values for normal growth and they fitted within a normal range of blood of normal chickens. Hemoglobin concentration (Hb) of Thai indigenous chickens was higher than both Thai indigenous crossbred and broiler chickens. White blood cells of heterophil of Thai indigenous crossbred chickens were higher than broiler chickens, whilst white blood of lymphocyte of female was higher than female. However, the differences found on hematological values of both male and female were not statistically significant. Daily feed intake/week and average daily gained weight increased/week of broiler chickens ranked the highest followed by Thai indigenous crossbred and the lowest was with Thai indigenous chickens.
Purification and Characterization of Trypsin Inhibitor from Momordica cochinchinensis Spreng. Seed and Its Effects to Spodoptera litura
Panida Arimatsu,Orawan Sawangsook
Asian Journal of Plant Sciences , 2012,
Abstract: This research was conducted to determine the effect of trypsin inhibitor on growth and development of Spodoptera litura. Trypsin Inhibitor from Momordica cochinchinensis (MMCTI) seed was extracted and purified using Ion Exchange Chromatography with CM-52 column. Trypsin Inhibitor Unit (TIU) was identified by using BApNA as substrate. The MMCTI showed 0.34 specific inhibitory units and mass numbers was 3165.03. In larval feeding studies, MMCTI were found to retard growth and development of S. litura. Larvae fed on diet containing MMCTI showed reduction in weight that was dose-dependent. Larval mortality ranged from 8 to 15% of the larval population. Reduction in pupal weight by 25% and pupal mortality by 27% were also observed with 12 TIU g-1 diet. Malformed adults (5-16%) were recorded as an effect of abnormal development. Fertility was affected as the egg-laying incidence reduced from 810 egg/female (control) to 298 egg/female (12 TIU g-1 diet). This result indicated that MMCTI is an efficient inhibitor of proteinases in the S. litura larvae. Being small peptide, it would be easy to express MMCTI in plants to confer protection against devastating pests such as S. litura.
The Role of Oxide Thin Layer in Inverted Structure Polymer Solar Cells  [PDF]
Orawan Wiranwetchayan, Zhiqiang Liang, Qifeng Zhang, Guozhong Cao, Pisith Singjai
Materials Sciences and Applications (MSA) , 2011, DOI: 10.4236/msa.2011.212226
Abstract: The role of wide band gap oxide thin layer in inverted structure polymer solar cells was investigated by employing oxide films of TiO2 and Nb2O5approximately 10 nm in thickness deposited onto FTO substrates. The experimental results demonstrated that the thin oxide layer serving to separate the electron collecting electrode and the photoactive film of a blend of poly(3-hexylthiophene) (P3HT) and phenyl-C61-butyric acid methyl ester (PCBM) was necessary to promote the formation of continuous uniform PCBM film to block holes in P3HT from being recombined with electrons in collecting electrode. A use of TiO2 buffer layer leads to power conversion efficiency as high as 2.8%. As for Nb2O5, in spite the fact that its conduction band is higher than the LUMO level of PCBM polymer acting as electron transport material, a power conversion of 2.7%, which was only slightly different from the 2.8% achieved for the cell employing TiO2. These experimental results suggest a tunneling mechanism for the electrons to transport from the PCBM to collecting electrode over the oxide film, instead of a diffusion through the oxide film arising from either energy or concentration difference of the photogenerated electrons.
Hematological, electrolyte and serum biochemical values of the Thai indigenous chickens (Gallus domesticus) in northeastern, Thailand
Suchint Simaraks,Orawan Chinrasri,Worapol Aengwanich
Songklanakarin Journal of Science and Technology , 2005,
Abstract: Thai indigenous chickens (Gallus domesticus) have been domesticated in rural villages in Thailand for a long time. These birds are important to low-income people who live in the rural part of Thailand. However, health problems have been a major cause limiting their population. Hematological, electrolyte and serum biochemical values, which are important for diagnosis of clinical signs and symptoms when affected by diseases, are limited. Blood samples from 40 chickens (20 males and 20 females) were used for hematological test while another 18 samples (from 10 males and 8 females) were analysed for electrolyte and serum biochemical values. The samples were obtained from Khon Kaen, Kalasin, Roi - Et, Maha Sarakham and Nakhon Ratchasima provinces, northeastern region of Thailand. The results revealed the following information: total red blood cell count, hemoglobin concentration, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, total white blood cell count, lymphocyte, heterophil, monocyte, eosinophil, basophil, H:L ratio values of Thai native chickens were 2.26 ± 0.29 × 106 cells/μl, 8.89 ± 1.20 g/dl, 32.18 ± 4.46%, 144.63 ± 18.61 fl, 39.69 ± 4.96 pg, 27.86 ± 3.37 g/dl, 2.04 ± 0.45 × 104 cells/μl, 63.68 ± 9.36%, 23.70 ± 7.21%, 4.20 ± 3.20%, 5.83 ± 3.53%, 2.65 ± 2.09% and 0.40 ± 0.17, respectively. Potassium, sodium and chloride values of Thai native chickens were 5.3 ± 0.8 mmol/l, 155.9 ± 3.1 mmol/l and 116.9 ± 2.7 mmol/l, respectively. Furthermore, serum biochemistry values of Thai native chickens such as total protein, glucose, alkaline phosphatase, uric acid, calcium and cholesterol were 4.6 ± 1.0 mg/dl, 190.2 ± 29.8 mg/dl, 235.9 ± 68.6 U/L, 5.0 ± 1.9 mg/dl, 10.4 ± 1.2 mg/dl and 102.4 ± 30.8 mg/dl, respectively. Besides, hemoglobin concentration, packed cell volume and eosinophil inthe males were significantly higher than in the females Thai indigenous chickens (P<0.05), lymphocyte counts of the females were significantly higher than the males (P<0.05). From serum biochemical values, potassium, sodium, total protein and uric acid of female indigenous birds were significantly higher than males (P<0.05).
Effect of Dietary Inclusion of Cassava Yeast as Probiotic Source on Growth Performance and Carcass Percentage in Japanese Quails
Songsak Chumpawadee,Orawan Chinrasri,Suwannee Santaweesuk
Pakistan Journal of Nutrition , 2009,
Abstract: Use of antibiotics as an additive in poultry diets to improve growth has been banned in several country. A popular alternative to the use of antibiotics has been use of probiotics. The study was conducted to investigate the effect of dietary inclusion of cassava yeast as a probiotic source on growth performance and carcass percentage in Japanese quails. Three hundred and twenty Japanese quails (1 day of age), were used. The Japanese quails were randomly allocated to 32 pens containing 10 birds each with 8 replicates and assigned to receive one of four dietary treatments (1.Control, 2. S. cerevisae 1x106 organisms/kg, 3. S. cerevisiae 1x107 organisms/kg, 4. S. cerevisiae 1x108 organisms/kg) in a completely randomized design. The results showed that feed intake, feed conversion ratio, average daily gain and carcass percentage were not significantly different among treatments (p>0.05). However, significant differences were observed in feed intake at weeks 5 and average daily gain at week 2. The results of the present experiment showed that dietary inclusion of cassava yeast as a probiotic to Japanese quail seems to have minimal influence on growth performance and carcass percentage.
Optimization of Nitrogen and Metal Ions Supplementation for Very High Gravity Bioethanol Fermentation from Sweet Sorghum Juice Using an Orthogonal Array Design
Orawan Deesuth,Pattana Laopaiboon,Prasit Jaisil,Lakkana Laopaiboon
Energies , 2012, DOI: 10.3390/en5093178
Abstract: Optimization of four parameters, i.e., zinc (Zn 2+), magnesium (Mg 2+), manganese (Mn 2+) and yeast extract for bioethanol production from sweet sorghum juice by Saccharomyces cerevisiae NP 01?under very high gravity (VHG, 270 g·L ?1 of total sugar) conditions was performed using an L 9 (3 4) orthogonal array design. The fermentation was carried out at 30 °C in 500-mL air-locked Erlenmeyer flasks at the agitation rate of 100 rpm and the initial yeast cell concentration in the juice was approximately 5 × 10 7 cells·mL ?1. The results showed that the order of influence was yeast extract > Mn 2+ > Zn 2+ > Mg 2+ and the optimum nutrient concentrations for the ethanol fermentation were Zn 2+, 0.01; Mg 2+, 0.05; Mn 2+, 0.04; and yeast extract, 9 g·L ?1. The verification experiments under the optimum condition clearly indicated that the metals and nitrogen supplementation improved ethanol production efficiency under the VHG fermentation conditions. The ethanol concentration ( P), yield ( Yp/s) and productivity ( Qp) were 120.58 ± 0.26 g·L ?1, 0.49 ± 0.01 and 2.51 ± 0.01 g·L ?1·h ?1, respectively, while in the control treatment (without nutrient supplement) P, Yp/s and Qp were only 93.45 ± 0.45 g·L ?1, 0.49 ± 0.00 and 1.30 ± 0.01 g·L ?1·h ?1, respectively.
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