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Search Results: 1 - 10 of 2837 matches for " Nwokoro PU "
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Hybrid Iteration Method for Fixed Points of Nonexpansive Mappings in Arbitrary Banach Spaces
Osilike MO,Isiogugu FO,Nwokoro PU
Fixed Point Theory and Applications , 2007,
Abstract: We prove that recent results of Wang (2007) concerning the iterative approximation of fixed points of nonexpansive mappings using a hybrid iteration method in Hilbert spaces can be extended to arbitrary Banach spaces without the strong monotonicity assumption imposed on the hybrid operator.
Influence of media composition on the production of alkaline α-amylase from Bacillus subtilis CB-18
Nwokoro Ogbonnaya,Anthonia Odiase
Acta Scientiarum Polonorum : Technologia Alimentaria , 2012,
Abstract: Background. Starch, a homopolysaccharide is an important and an abundant food reserve and energy source. Starches are processed to yield different products which find many industrial applications. Alpha-amylases hydrolyze starch by cleaving α-1,4-glucosidic bonds and have been used in food, textile and pharmaceutical industries [Sun et al. 2010]. Enzymatic conversion of starch with amylase presents an economically superior alternative to the conventional method of starch gelatinization. Alkaline α-amylase has an important position in the global enzyme market as a constituent of detergent. In this paper, we screened soil bacteria and an isolate, alkalophilic Bacillus subtilis CB-18 was found to produce an alkaline α-amylase in different media. Material and methods. Screening of the isolates for amylolytic activity was carried out by growing bacteria isolated from the soil in starch agar plates and subsequently staining the plates with iodine solution to reveal zones of hydrolysis of starch. The selected isolate, Bacillus subtlis CB-18 was grown in different media at alkaline pH to evaluate the influence of media composition on alkaline α-amylase production. Enzyme assay was carried out by growing the culture in a broth medium and obtaining cell – free culture supernatant after centrifugation at 2515 × g for 15 minutes Amylase activity was determined by incubating 0.5 ml of crude enzyme solution in 0.1M Tris/HCl buffer (pH 8.5) with 0.5 ml of 1% soluble starch solution. The reaction was terminated by the addition of DNS reagent and reducing sugar produced from the amylolytic reaction was determined. Results. Bacillus subtilis CB-18 used for this work was selected because it produced 7 mm zone diameter on starch agar plate. This organism was cultured in different alkaline broth media containing 2% soluble starch as inducer carbohydrate for α-amylase production. Among the carbon sources used for enzyme production, sorbitol was the best to stimulate enzyme production with α-amylase activity of 758 U/mL after 48 h. Peptone was the best nitrogen source for enzyme production with α-amylase activity of 680 U/mL after 48 h. Metal ions including Ca 2+, Mn2+ and Mg2+ stimulated enzyme production while Hg2+ and Ag+ repressed enzyme production. The best enzyme yields were observed in basal media containing agro-based substrates. Conclusion. This work reports the production of alkaline α-amylase by Bacillus subtlis CB-18 in different media. Enzyme production was highest when agro-based media were used to formulate the media.
Studies on Akamu, a traditional fermented maize food Estudios en Akamu, una comida tradicional de maiz fermentado
Nwokoro Ogbonnaya,Chukwu Bernice Chidinma
Revista Chilena de Nutricíon , 2012,
Abstract: Microbiological and some physicochemical properties of akamu, a fermented maize food were studied. Microbial population, pH, titratable acidity, protein, sugar and starch were measured during the fermentation. The initial microflora consisted ofa heterogenous mixture of microorganisms namely Lactobacillus delbrueckii, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus amylovorus, Pseudomonas aeruginosa, Pseudomonas alkaligenes, Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Candida utilis, Candida tropicalis, Saccharomyces cerevisiae, Aspergillus oryzae, Aspergillus niger, Penicillium citrinum, Rhizopus micros-porus and Rhizopus oligosporus. Within 24 h, lactobacilli and aerobic mesophilic bacteria accounted for a major portion of the total microflora. The lactobacilli numbers increased from 1.6 x107 cfu/g after24 h to 7.1 x108 cfu/g after 72 h. Total aerobic mesophilic bacteria increased from 2.5 x109 cfu/g after 24 h to 4.2 x108 cfu/g after 72 h. Enterobacteriaceae count decreased from 6.3 x 103 cfu/g after 24 h to 2.5x102 cfu/g after 72 h, although this level remained significantly high for a finished food product. Yeasts increased steadily and reached 6.8 x105 cfu/g after 72 h. Mould counts decreased from 6.3 x103 cfu/g after 24 h to 1.3 x102 cfu/g after 72 h. Moulds were largely responsible for amylolytic activity in pure culture. Fermentation caused a general decrease in pH from 6.6 to 3.9 after 72 h and titratable acidity increased from 0.48 to 0.79 after 72 h. Starch concentration decreased from 68 g/100 g to 37.4 g/100g. Protein and reducing sugar concentrations increased from 12.8 g/100 g to 18.5 g/100 g and from 5.3 g/100 g to 17.6 g/100 g, respectively. The types and numbers of microorganisms isolated from akamu could pose a health risk to consumers especially where this food product is used as a weaning product for infants. Se estudiaron las propiedades microbiológicas y fisicoquímicas del akamu, un alimento fermentado a base de maíz. Se midió la población microbiológica, el pH, la acidez por titulación, azúcar y almidón durante la fermentación. La microflora inicial consistía en una mezcla heterógenea de microrganismos denominados Lactobacillus delbruecki, Lactobacillus plantarum, Lactobacillus fermentum, Lactoba-cillus amylovorus, Pseudonomas aeruginosa, Pseudomonas alkaligenes, Bacillus cereus, Bacillus licheniformis, Bacillus subtilis, Candida utilis, Candida tropicvallis, Saccharomyces cerevisiae,Aspergillus oryzae, Aspergillus niger, Penicillium citrinum, Rhizopus microsporus y Rhizopus oligosporus. Al cabo de
Linamarase Enzyme from Lactobacillus delbrueckii NRRL B-763: Purification and Some Properties of a B-Glucosidase
Ogbonnaya Nwokoro,Florence Onyebuchi Anya
Revista de la Sociedad Química de México , 2011,
Abstract: Se estudiaron algunas propiedades bioquímicas y la purificación de la enzima linamarasa obtenida de Lactobacillus delbrueckii NRRL B-763. La enzima cruda fue usada para eliminar los compuestos cianogénicos de harina de yuca. Muestras de 150 mm en el tama o de la partícula tratadas con la enzima cruda mostraron una reducción del 95% en el contenido de estos compuestos tóxicos después de 20 horas de tratamiento. Las muestras sin tratar, testigo, de 0.5 mm de tama o de partícula de sólo el 5.7% después de 40 horas. La enzima fue purificada 33 veces con un rendimiento del 40% a través de una serie de 4 pasos, precipitación con sulfato de amonio y con acetona, sucesivamente, cromatografía en columna de intercambio iónico y cromatografía de filtración en gel con Sephadex G-200. El pH óptimo de la enzima purificada fue de 4.5. La enzima es estable 100% en el rango de pH de 5.0 a 6.0. La actividad máxima de la enzima se observó a una temperature de 50 °C. La masa molecular aproximada de la enzima es de 56 kDa calculada a partir de su tiempo de elusión de una columna de permeasión en gel con Sephadex G-200. La enzima linamarasa podría ser adaptada para mejorar la degradación de los compuestos cianogénicos de la yuca, así como para otras aplicaciones biotecnológicas.
Sustainable or Green Construction in Lagos, Nigeria: Principles, Attributes and Framework
Immaculata Nwokoro,Henry Ndubuisi Onukwube
Journal of Sustainable Development , 2011, DOI: 10.5539/jsd.v4n4p166
Abstract: The concept of sustainable development is used as a basis for enhancing understanding of sustainable construction. Principles of sustainable construction cover four attributes: social, economic, biophysical and technical. The research examined these concepts, principles and attributes in understanding sustainable and green construction as well as current practices and challenges of sustainable construction in Lagos, Nigeria. The research embraced both quantitative and qualitative methods of data collection. The sample frame is the total number of built industry registered and practising professionals in Lagos. A total number of 85 respondents were randomly selected for study from each group. A 5-point likert scale was used to assess respondents’ judgement on the identified social, economic, bio-physical and technical indicators. Focus group discussions (FGDs) were also conducted with all the above professional groups to corroborate the primary information. For a wider coverage, three different construction sites are selected to reflect income neighbourhoods-Lekki (high income), Yaba (medium income) and Bariga (low income). Data Collected were analysed using the mean item score. A multi- stage framework which required the application of environmental assessment and environment management systems for construction projects was utilised. Research findings indicate that the most important factors considered for sustainable construction with their mean item scores are quality of working conditions (0.852) strengthening and enforcement of relevant law and regulations(0.872), encouraging construction waste management(0.819) and design for flexibility and adaptability. Results from the FGDs indicate that the current practice on sustainable construction does not take into consideration integrated design process, acoustic and visual comfort in the planning and construction of sustainable projects. The research therefore concludes that government should improve existing laws to enhance quality of working life, education, training as well as knowledge management for all stakeholders in sustainable construction.
Strategy & Techniques for Restoration of Healthy Aquaecosystem from Toxic Super Eutrophic Water Body  [PDF]
Peimin Pu, Jiangping Pu
Journal of Geoscience and Environment Protection (GEP) , 2015, DOI: 10.4236/gep.2015.36009

There are complex river-lake systems in the Taihu Lake catchment with total water surface area of 6174.7 km2, and population density of 1079/km2, including Taihu Lake water surface area of 2338 km2. The water systems in this catchment have healthy aquaecosystems during long history. However, in some riverlets in this catchment the water quality was estimated as “acute toxicity for higher organisms” and over standards for many heavy metal elements content; there were no any living plants and macro organisms in the water body, because there were developed a series of industry with abundant release of heavy metals and difficult decomposition organic chemical components along the riverlets during last decades. The even more serious situation was observed in sediments of the riverlets. How to restore such riverlet into a healthy aquaeosystem with abound plants and higher organisms? The main strategy and techniques are described in this paper as summarizing a report of engineering in a riverlet in Wuxi New District during last years, which leads to restore the aquaecosystem into a healthy one with abundant surface plant cultured on floating islands and observed living fish, lobster, frog, toad, mollusk and others in the riverlet. The main techniques are: 1) softwall buffer technic; 2) floating eco-island technic by using which can culture any plant which can be cultured in solution; 3) immobilized nitrogen cycle bacteria (INCB) technic; 4) tattering esters and other big-molecule organic chemicals by using electronic pulse technic and photosensitization technic; 5) mist spray facility technic for improving dissolved oxygen in deep water layers; 6) technic for buffering and suppressing H2S release from water; 7) the appropriate portion of surface with cultured plant to the total water surface area is about 1/3; 8) Cress [Oenanthe Ljavanica (Bl.) DC.] and Myriophyllum verticilatum L. may be cultured in Taihu Lake catchment during the whole year as main plants with mosaic combination of other supplement plants in different seasons.

Rectal bleeding amongst Medical Students: Prevalence and Consultation Behaviour.
OT Adedayo, AS Babatunde, OA Adekoya, CC Nwokoro
East and Central African Journal of Surgery , 2011,
Abstract: Background: Rectal bleeding can be a symptom of colorectal cancer. Consultation behaviour of health care professionals may influence the attention they give to patients who consult them. Objective: To determine the prevalence of rectal bleeding among medical students and their consultation behaviour. A Questionnaire survey was undertaken at Olabisi Onabanjo University Teaching Hospital, Sagamu, Nigeria. Methods: One hundred and eighty-seven final year medical students completed the questionnaires. Results: Forty-one (21.9%) had noticed rectal bleeding at sometime in their lives and 27 (14.4%) had noticed it within the previous 12 months. Five out of twenty-seven (18.5%) sought medical advice. Those who sought medical advice (consulters) perceived the symptom as serious. Non-consulters felt they knew the cause of their symptoms and some were afraid of rectal examination. Only 40% of consulters had digital rectal examination conducted by the consulted physician. Conclusion: Rectal bleeding is relatively common amongst the medical students reviewed. Only one in six consulted a physician.
Anthropometric Assessment of Nutritional Status and Growth of 10 - 20 Years Old Individuals in Benin City (Nigeria) Metropolis
Nwokoro,Smart O.,K. Ifada,O. Onochie
Pakistan Journal of Nutrition , 2006,
Abstract: Anthropometric assessment of the nutritional status and growth of 2,012 randomly selected males and females between the ages of 10 - 20 years was carried out by cross-sectional method in Benin City Metropolis. Anthropometrical indices considered were weight, height and arm circumference. Percentile values (10th, 50th, 90th) which represent the growth standards of males and females were established from this study. Comparisons were made by comparing the 50th centile curves for height and weight of males and females obtained from this study with those of the WHO/NCHS standards. The results of the comparison revealed that the 50th centile curves of subjects from Benin City consistently lagged behind those of WHO/NCHS standards for all the anthropometric variables considered, except the 50th centile curve of females for height which showed that the 50th centile curve of females from Benin City compared favourably with the WHO/NCHS standards, and do not fall below them. The interrelationship between the various anthropometric variables revealed that the correlation coefficients for males and females respectively were: Age and Weight (0.99 and 1.0), Age and Arm circumference (0.95 and 0.99), Height and Arm circumference (0.91 and 0.97) and,(0.97 and 0.98) for Weight and height. The age of puberty was determined from this study to be 15.5 years for females and 18 years for males.
Research on College Students’ Conformity in Sports  [PDF]
Pu Liu
Creative Education (CE) , 2016, DOI: 10.4236/ce.2016.73045
Abstract: As a social member, college student will inevitably be influenced by the other students and the society as a whole. Conformity is a psychological term that indicates the influence. It means that an individual tends to keep accordance with the majority under direction or pressure. Conformity is a common phenomenon among college students when they are having PE class or playing sports after class, which has a great effect on students’ physical activities, sport attitude and philosophy of life. This paper analyzes the effects of conformity on college students in sports and gives some practical suggestions to PE instructors about how to make use of conformity to help students change their attitude to sports and form lifelong sports habit.
Uterine incision closure at caesarean section: A randomised comparative study of intraperitoneal closure and closure after temporary exteriorisation
OC Ezechi, BKF Kalu, FO Njokanma, CA Nwokoro, GCE Okeke
West African Journal of Medicine , 2005,
Abstract: Background: The safety of the technique of uterine exteriorization at caesarean section though popular among obstetricians, remains controversial. Objective: To evaluate the influence of exteriorization of uterus during uterine repair on caesarean morbidity. Methods: A randomized comparative study of 136 women undergoing primary caesarean delivery at Havana Specialist Hospital Lagos Nigeria. Data on operation time, estimated blood loss, postoperative morbidities were collected and analysed with comparison between the two groups using chi square, Fischer's exact test and t-test as appropriate. Results: The mean operative time, estimated blood loss, transfusion rate and postoperative anemia rate were significantly less in the exteriorized group than the intraperitoneal group (p= 0.000, 0.009,0.048 0.038and 0.028 respectively), but not in other outcome measures. Conclusion: With shorter operative time, less blood loss and similar morbidity profile exteriorization of uterus during caesarean section seems to be preferred except where it is not possible because of adhesions and surgeons inexperience.
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