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Search Results: 1 - 10 of 404172 matches for " Novica M. Mili?evi? "
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Chagasic Thymic Atrophy Does Not Affect Negative Selection but Results in the Export of Activated CD4+CD8+ T Cells in Severe Forms of Human Disease
Alexandre Morrot ,Eugênia Terra-Granado,Ana Rosa Pérez,Suse Dayse Silva-Barbosa,Novica M. Milievi,Désio Aurélio Farias-de-Oliveira,Luiz Ricardo Berbert,Juliana De Meis,Christina Maeda Takiya,Juan Beloscar,Xiaoping Wang,Vivian Kont,P?rt Peterson,Oscar Bottasso,Wilson Savino
PLOS Neglected Tropical Diseases , 2011, DOI: 10.1371/journal.pntd.0001268
Abstract: Extrathymic CD4+CD8+ double-positive (DP) T cells are increased in some pathophysiological conditions, including infectious diseases. In the murine model of Chagas disease, it has been shown that the protozoan parasite Trypanosoma cruzi is able to target the thymus and induce alterations of the thymic microenvironment and the lymphoid compartment. In the acute phase, this results in a severe atrophy of the organ and early release of DP cells into the periphery. To date, the effect of the changes promoted by the parasite infection on thymic central tolerance has remained elusive. Herein we show that the intrathymic key elements that are necessary to promote the negative selection of thymocytes undergoing maturation during the thymopoiesis remains functional during the acute chagasic thymic atrophy. Intrathymic expression of the autoimmune regulator factor (Aire) and tissue-restricted antigen (TRA) genes is normal. In addition, the expression of the proapoptotic Bim protein in thymocytes was not changed, revealing that the parasite infection-induced thymus atrophy has no effect on these marker genes necessary to promote clonal deletion of T cells. In a chicken egg ovalbumin (OVA)-specific T-cell receptor (TCR) transgenic system, the administration of OVA peptide into infected mice with thymic atrophy promoted OVA-specific thymocyte apoptosis, further indicating normal negative selection process during the infection. Yet, although the intrathymic checkpoints necessary for thymic negative selection are present in the acute phase of Chagas disease, we found that the DP cells released into the periphery acquire an activated phenotype similar to what is described for activated effector or memory single-positive T cells. Most interestingly, we also demonstrate that increased percentages of peripheral blood subset of DP cells exhibiting an activated HLA-DR+ phenotype are associated with severe cardiac forms of human chronic Chagas disease. These cells may contribute to the immunopathological events seen in the Chagas disease.
Determination of the optimal conditions for obtaining potassium nitro-humate from lignite
Milievi? Zoran M.,Petrovi? Petar R.
Hemijska Industrija , 2004, DOI: 10.2298/hemind0401019m
Abstract: Raw lignite is enriched by humic acids by oxidation with nitric acid. The optimal conditions for producing potassium nitro-humate were defined by investigating the level of neutralization of the oxidized lignite, by means of potassium hydroxide. The increased content of humic acids and potassium in potassium nitro-humate makes it applicable as an organic-mineral fertilizer.
Comparison, artificial neural network modeling and genetic algorithm optimization of the resinoid and potassium yields from white lady’s bedstraw (Galium mollugo L.) by conventional, reflux and ultrasound-assisted aqueous-ethanolic extraction
Mili? Petar S.,Rajkovi? Katarina M.,Milievi? Predrag M.,Mili? Slavica M.
Chemical Industry and Chemical Engineering Quarterly , 2013, DOI: 10.2298/ciceq120316049m
Abstract: In this work, the yields of resinoid and potassium obtained from aerial parts of white lady’s bedstraw (Galium mollugo L.) by maceration, reflux extraction and ultrasound-assisted extraction using aqueous ethanol solutions as solvents. The main goal was to define the influence of the extraction technique and the ethanol concentration on the resinoid and potassium yields. The resinoid and potassium yields were determined by the solvent evaporation from the liquid extracts to constant weight and the AAS emission method, respectively. The dependence of resinoid and potassium yields on the ethanol concentration was described by linear and quadratic polynomial models, respectively. The best potassium extraction selectivity of 0.077 g K/g of dry extract was achieved by maceration at the ethanol concentrations of 10 g/100 g. The artificial neural network (ANN) was successfully applied to estimate the resinoid and potassium yields based on the ethanol concentration in the extracting solvent and the time duration for all three extraction techniques employed. The response surface methodology was also used to present the dependence of ANN results on the operating factors. The extraction process was optimized using the ANN model coupled with genetic algorithm. The maximum predicted resinoid and potassium yields of 30.4 and 1.67 g/100 g of dry plant were obtained by the ultrasonic extraction (80 min) using the 10 g/100 g aqueous ethanol solution.
A study of n-alkane type biomarkers in coal
Milievi? Zoran M.,Petrovi? Milena M.,Petrovi? Petar R.
Hemijska Industrija , 2003, DOI: 10.2298/hemind0301019m
Abstract: The isolation of saturated hydrocarbons from free bitumen coal and also from bound bitumen coal in the region of Kosovo is descriled in this study. n-Alkanes from C17-C21 to C33-C35, with A7-C29 being dominant, were identified by gaschromatographic analysis of the free bitumen. This distribution indicated the terrestrial origin of the coals and a low level of carbonification. The decreasing values of the CPI of the bound bitumen (1.08-2.77), composed to the CPI values of the free bitumen (2,04-3,90) and a shift of the dominant n-alkane (A7-C29 -> A7-C23) are a result of the higher "maturity" level of the n-alkanes in bound bitumen.
Congenital psedoarthrosis of the tibia treated with the free microvascular fibula
Bumba?irevi? Marko,Brdar R.,?uki? V.,MilieviM.
Acta Chirurgica Iugoslavica , 2005, DOI: 10.2298/aci0502121b
Abstract: Congenital pseudoarthrosis (CPT) of the lower leg is still a considerable therapeutic problem. There are many surgical and other procedures to manage pseudoarthrosis, but the results are not always satisfactory, unfortunately, and are accompanied by a big number of complications, even limb amputation in some cases. The most complicated surgical procedure is transplantation of the autogenous free microvascular fibula coil which has had the best results momentarily. We are showing a case of a 2.5- year old patient with congenital pseudoarthrosis of the lower leg previously treated unsuccessfully by a conventional surgical method. This is at the same time the first case of such operative treatment of CPT in the territory of Serbia and Montenegro as well as former Yugoslavia. The patient was operated in October 1992.
Transcatether chemoembolization in the treatment of hepatocellular carcinoma
Ma?ulovi? D.,MilieviM.,Markovi? ?.,?aranovi? ?.
Acta Chirurgica Iugoslavica , 2009, DOI: 10.2298/aci0904139m
Abstract: The authors describe their own experience with chemoembolization as a palliation in the treatment of non resectable hepatocellular carcinoma. Material/Methods: During period of 64 months procedure was performed in 41 patients with non resectable hepatocellular carcinoma. The combination of Lipiodol and chemotherapeutic agents were applied in a. hepatica propria and its branches via transfemorally placed catheter. Stages of neoplasms were defined by Okuda method. Results: The majority of tumors (30) were classified as Grade I. Liver cirrhosis was present in 36 patients, and abnormal levels of alpha-fetoprotein were found in 68% of cases. Each of twenty nine patients had more than one chemoembolization therapy, therefore, a total of 85 treatments were carried out. CT scanning perfomed one month following the procedure revealed more than 75% of Lipiodol retention in 42% of cases, and over 90% of neoplasm necrosis was recorded in 90% of cases, while all treated patients manifested lower levels of alpha - fetoprotein. All patients survived during three and six months, respectively, while the survival rate was 68% after 18 months. No letal outcome was reported during procedure, and morbidity in relation to total number of interventions was 19%. Conclusion : Achieved effects of this relatively safe procedure in our series do not differ significantly from those in the literature.
The future of transcatheter antitumor therapy
Ma?ulovi? D.,MilieviM.,Stevi? R.,Sagi? D.
Acta Chirurgica Iugoslavica , 2009, DOI: 10.2298/aci0904135m
Abstract: Transcatheter antitumor therapy very quickly accepted during the last decade and their importance in the treatment of oncology patients will be increasing. By improvement of new targeted agents, which can be given intraarterial or systemic, efficiency of transcatheteric therapeutic approaches can be drastically increased. Numerous clinical trials (study phase I / II / III) relating to the synergy of two antitumor therapeutic approaches are already in progress. Preliminary results of these trials are already very encouraging. Further improvement in the development of specific therapeutics antitumor drugs and systemic applications will be a big step in the quest for medication against malignant tumors.
Modern aspects of the ankle fracture treatment
Le?i? Aleksandar,Tomi? S.,?obelji? G.,MilieviM.
Acta Chirurgica Iugoslavica , 2005, DOI: 10.2298/aci0502023l
Abstract: The ankle fractures continue to be a topical issue in orthopedic surgery. X-ray diagnostics, but primarily also other modern diagnostic procedures such as CT, MRI, and arthroscopy enable detection of not only fractures but also osteocartilaginous fractures and soft-tissue ligamentary lesions, which are frequent causes of pain and instability of the ankle. The key segment is the posterio-lateral segment and tibio-fibular syndesmosis whose integrity is sometimes only surgically establishable. In the ankle treatment, stable fixation - since recently by means of resorptive osteofixation materials - and early rehabilitation of the operated ankle are aimed at. The open and pylon fractures, as the most severe forms of ankle fractures, are treated by external fixation with minimum internal fixation (hybrid fixation) of the ankle with conversion of the rigid into a dynamic (articulated) external fixator enabling movement and nutrition of the damaged articular cartilage.
Paleomagnetism of the Late Paleogene and Neogene rocks of the Serbian Carpatho-Balkanides: Tectonic implications
Marovi? Milun,?okovi? Ilija M.,Milievi? Vlada,Tolji? Marinko
Geolo?ki Anali Balkanskog Poluostrva , 2002, DOI: 10.2298/gabp0264001m
Abstract: This paper focuses on results of the paleomagnetic research conducted in the territory of Serbian Carpatho-Balkanides, within the following basinal domains: Babu nica-Koritnik (the Oligocene deposits), agubica (the upper part of the Lower Miocene and the lower part of the Middle Miocene deposits) and Dacian (the Pontian deposits). The clockwise rotations are established for each basinal region: Babu nica-Koritnik 10°, agubica 5-10° and Dacia 5-20°. On the basis of these results and the available data on rotations in the Romanian Southern Carpathians, new tectonic implications are discussed as a contribution to understanding the genesis of the Paleogene-Neogene and Neogene fabric of the Carpatho-Balkanides in Serbia. It is shown that this region has been subjected not only to vertical, but also to horizontal mobility during the latest stage of its geological development.
The importance of genus Candida in human samples
Boji?-Milievi? Gordana M.,Mikov Momir M.,Golo?orbin-Kohn Svetlana M.
Zbornik Matice Srpske za Prirodne Nauke , 2008, DOI: 10.2298/zmspn0814079b
Abstract: Microbiology is a rapidly changing field. As new researches and experiences broaden our knowledge, changes in the approach to diagnosis and therapy have become necessary and appropriate. Recommended dosage of drugs, method and duration of administration, as well as contraindications to use, evolve over time all drugs. Over the last 2 decades, Candida species have emerged as causes of substantial morbidity and mortality in hospitalized individuals. Isolation of Candida from blood or other sterile sites, excluding the urinary tract, defines invasive candidiasis. Candida species are currently the fourth most common cause of bloodstream infections (that is, candidemia) in U.S. hospitals and occur primarily in the intensive care unit (ICU), where candidemia is recognized in up to 1% of patients and where deep-seated Candida infections are recognized in an additional 1 to 2% of patients. Despite the introduction of newer anti-Candida agents, invasive candidiasis continues to have an attributable mortality rate of 40 to 49%; excess ICU and hospital stays of 12.7 days and 15.5 days, respectively, and increased care costs. Postmortem studies suggest that death rates related to invasive candidiasis might, in fact, be higher than those described because of undiagnosed and therefore untreated infection. The diagnosis of invasive candidiasis remains challenging for both clinicians and microbiologists. Reasons for missed diagnoses include nonspecific risk factors and clinical manifestations, low sensitivity of microbiological culture techniques, and unavailability of deep tissue cultures because of risks associated with the invasive procedures used to obtain them. Thus, a substantial proportion of invasive candidiasis in patients in the ICU is assumed to be undiagnosed and untreated. Yet even when invasive candidiasis is diagnosed, culture diagnosis delays treatment for 2 to 3 days, which contributes to mortality. Interventions that do not rely on a specific diagnosis and are implemented early in the course of Candida infection (that is, empirical therapy) or before Candida infection occurs (that is, prophylaxis) might improve patient survival and may be warranted. Selective and nonselective administration of anti-Candida prophylaxis is practiced in some ICUs. Several trials have tested this, but results were limited by low statistical power and choice of outcomes. Thus, the role of anti-Candida prophylaxis for patients in the ICU remains controversial. Initiating anti-Candida therapy for patients in the ICU who have suspected infection but have not responded to antib
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