oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Any time

2019 ( 6 )

2018 ( 15 )

2017 ( 12 )

2016 ( 7 )

Custom range...

Search Results: 1 - 10 of 6352 matches for " Niubys Cayado Gutiérrez "
All listed articles are free for downloading (OA Articles)
Page 1 /6352
Display every page Item
Estudio del reordenamiento molecular de los genes TEL/AML1 en la leucemia linfocítica aguda: Resultados preliminares Study of the molecular rearrangement of TEL/AML 1 genes in acute lymphocytic leukemia: Preliminary results
Niubys Cayado Gutiérrez,Adriana Mu?iz Fernández,Alejandro González Otero,Eva Svarch
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: La leucemia linfocítica aguda (LLA) representa aproximadamente el 80 % dentro de las leucemias pediátricas. Recientemente se ha demostrado por biología molecular la existencia de una translocación críptica, la t (12;21) (p12;q22) en la LLA de tipo B, que no se detecta por las técnicas citogenéticas convencionales e involucra los oncogenes TEL y AML1. Esta alteración es actualmente la más común en esta leucemia y se observa aproximadamente en el 25 % de los casos. Diversos investigadores han planteado que dicha translocación identifica a un subgrupo de pacientes con una evolución muy favorable, por lo que se considera un indicador de buen pronóstico. La determinación de la t (12;21) en el estudio de LLA tiene importancia pronóstica, además de servir como marcador para la detección de la enfermedad mínima residual. En nuestro trabajo estandarizamos la técnica de RT-PCR para la detección de la t (12;21) y además analizamos muestras de 20 pacientes pediátricos con LLA tipo B, que en el momento del estudio se encontraban en la fase de diagnóstico inicial o en recaída. En el estudio, 5 de los 20 pacientes mostraron reordenados los genes TEL/AML 1, lo que representa el 25 % de los casos. Esta frecuencia concuerda con lo comunicado en la literatura hasta el momento Acute lymphocytic leukemia (ALL) represents approximately 80% of the pediatric leukemias.The existance of a cryptic translocation, the t (12;21) (p12;q22) in the type B ALL, which is not detected by the conventional cytogenetic techniques and involve the TEL and AML1 oncogenes, has been recently shown by molecular biology. This alteration is at present the most common in this leukemia and it is observed approximately in 25% of the cases. Some authors have stated that such translocation identifies a group of patients with a very favorable evolution and that's why it is considered as an indicator of good prognosis. The determination of the t (12;21) in the study of ALL has a prognostic importance and it also serves as a marker for the detection of the minimal residual disease. In our paper, we standardized the RT-PCR technique for the detection of the t (12;21) and we also analized samples from 20 pediatric patients with type B ALL, which at the time of the study was in the phase of initial diagnosis or on relapse. In the study, 5 of the 20 patients showed rearranged TEL/AML l genes, which accounted for 25% of the cases. This frequency agrees with what is reported in literature up to now
Estudio del reordenamiento molecular de los genes TEL/AML1 en la leucemia linfocítica aguda: Resultados preliminares
Cayado Gutiérrez,Niubys; Mu?iz Fernández,Adriana; González Otero,Alejandro; Svarch,Eva; Martínez Antu?a,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: acute lymphocytic leukemia (all) represents approximately 80% of the pediatric leukemias.the existance of a cryptic translocation, the t (12;21) (p12;q22) in the type b all, which is not detected by the conventional cytogenetic techniques and involve the tel and aml1 oncogenes, has been recently shown by molecular biology. this alteration is at present the most common in this leukemia and it is observed approximately in 25% of the cases. some authors have stated that such translocation identifies a group of patients with a very favorable evolution and that's why it is considered as an indicator of good prognosis. the determination of the t (12;21) in the study of all has a prognostic importance and it also serves as a marker for the detection of the minimal residual disease. in our paper, we standardized the rt-pcr technique for the detection of the t (12;21) and we also analized samples from 20 pediatric patients with type b all, which at the time of the study was in the phase of initial diagnosis or on relapse. in the study, 5 of the 20 patients showed rearranged tel/aml l genes, which accounted for 25% of the cases. this frequency agrees with what is reported in literature up to now
Diagnóstico molecular de la leucemia aguda promielocítica: Resultados preliminares Molecular diagnosis of acute promyelocytic leukemia: preliminary results
Gisela Martínez Antu?a,Niubys Cayado Gutiérrez,Adriana Mu?iz Fernández,Edgardo Espinosa Martínez
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: La leucemia aguda promielocítica (LAP) se caracteriza por la presencia de la translocación recíproca t (15;17) que tiene como resultado la formación del gen híbrido PML-RARa . Como la LAP se considera una emergencia hematológica y además tiene hoy en día un tratamiento muy específico con ácido retinoico (ATRA), es muy importante hacer un diagnóstico rápido y preciso, que en muchos casos permite incluso salvar la vida del paciente. En la actualidad se han desarrollado métodos de RT-PCR para detectar el gen híbrido PML-RARa . Estas técnicas moleculares han sido extremadamente útiles en el diagnóstico de esta entidad. En este trabajo presentamos los resultados preliminares del diagnóstico molecular en 38 pacientes con LAP. En 36 pacientes se demostró la presencia del gen híbrido y 2 fueron negativos. Del total de enfermos con resultados positivos, 19 (55 %) fueron bcr 1, 2 (5 %) fueron bcr 2 y 14 (40 %) fueron bcr 3. Todos los pacientes con resultados positivos respondieron al tratamiento con ATRA. En 1 de los 2 pacientes con resultados negativos se demostró la presencia de la t(11;17). Ninguno de estos 2 enfermos respondió al tratamiento con ATRA Acute promyelocytic leukemia (APL) is characterized by the reciprocal translocation t(15; 17) that results in fusion gene PML-RARa formation. As APL is considered to be an hematological emergency and also is given a very specific treatment with retinoic acid (ATRA), then it is very important to diagnose quickly and accurately which makes it possible in many cases to save the patient's life. At present RT- PCR methods have been developed to detect PML-RARa gen. These molecular techniques have been extremely useful in diagnosing this entity. This paper sets forth the preliminary results of molecular diagnoses of APL in 38 patients. 36 cases presented the fusion gene and 2 did not. Of the total number of positive patients, 19(55%) were bcr 1, 2 (5%) were bcr 2 and 14(40%) bcr 3. All the patients with positive results were responsive to treatment with ATRA. One of the two patients with negative results showed the existence of t (11; 17). These two patients did not respond to treatment with ATRA
Posible quimera M3: M5 en un paciente con leucemia aguda promielocítica; diferente respuesta clonal al tratamiento con ANTRA y quimioterapia Probable chimera M3: M5 in an acute promyelocytic leukemia patient; different clonal response to treatment with transretinoic acid and chemotherapy
Niubys Cayado Gutiérrez,Carlos Hernández Padrón,Luis Gabriel Ramón Rodríguez,Porfirio Hernández Ramírez
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2004,
Abstract: Se presenta un caso con leucemia aguda promielocítica (LAP) en el que se demostró la presencia del gen híbrido PML/RARa y la duplicación interna en tandem del gen FLT3 (DIT/FLT3) al momento del diagnóstico. Después de recibir tratamiento de inducción con ácido transretinoico (ATRA) y quimioterapia, el estudio citomorfológico de la médula ósea mostró una transformación a leucemia monocítica aguda (LMA-M5). En el estudio molecular desapareció el transcripto PML/RARa, pero se mantuvo la DIT/FLT3. Estos resultados sugieren la coexistencia de 2 clones leucémicos independientes, un clon promielocítico (M3) con el gen quimérico PML/RARa y otro monocítico (M5) con DIT/FLT3. Aunque la evolución hematológica y molecular apoya esta sugerencia, no se puede excluir la presencia de la DIT/FLT3 en el clon M3, pues no es un marcador específico de la LMA-M5 A case of acute promyelocytic leukemia in which the presence of hybrid PML/RAR a gene and the internal tandem duplication of FLT3 (DIT/FLT3)were demonstrated at the time of diagnosis was reported in this paper. After the induction treatment with transretinoic acid and chemotherapy, the cytomorphological study of the bone marrow revealed some change into acute monocytic leukemia (AML-M5). The molecular study revealed that PML/RARa had disappeared but ITD/FLT3 remained. These results suggest that two independent leukemia clones co-exist, that is, one promyeolytic (M3) with chimeric gene PML/RARa and the other monocytic (M5) with ITD/FLT3). Although the hematological and molecular evolution supports such a suggestion, the presence of ITD/FLT3 in clone M3 can not be ignored because it is not a specific marker for AML-M5
Frecuencia de los transcriptos BCR-ABL en pacientes cubanos con leucemia mieloide crónica Frequency of BCR-ABL transcripts in Cuban patients with chronic myeloid leukemia
Ana María Amor-Vigil,Yarienis González-Medina,Niubys Cayado-Gutiérrez,Gisela Martínez-Antu?a
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2012,
Abstract:
Posible quimera M3: M5 en un paciente con leucemia aguda promielocítica; diferente respuesta clonal al tratamiento con ANTRA y quimioterapia
Cayado Gutiérrez,Niubys; Hernández Padrón,Carlos; Rodríguez,Luis Gabriel Ramón; Hernández Ramírez,Porfirio; Martínez Antu?a,Gisela;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2004,
Abstract: a case of acute promyelocytic leukemia in which the presence of hybrid pml/rar a gene and the internal tandem duplication of flt3 (dit/flt3)were demonstrated at the time of diagnosis was reported in this paper. after the induction treatment with transretinoic acid and chemotherapy, the cytomorphological study of the bone marrow revealed some change into acute monocytic leukemia (aml-m5). the molecular study revealed that pml/rara had disappeared but itd/flt3 remained. these results suggest that two independent leukemia clones co-exist, that is, one promyeolytic (m3) with chimeric gene pml/rara and the other monocytic (m5) with itd/flt3). although the hematological and molecular evolution supports such a suggestion, the presence of itd/flt3 in clone m3 can not be ignored because it is not a specific marker for aml-m5
Diagnóstico molecular de la leucemia aguda promielocítica: Resultados preliminares
Martínez Antu?a,Gisela; Cayado Gutiérrez,Niubys; Mu?iz Fernández,Adriana; Espinosa Martínez,Edgardo; Dorticós Balea,Elvira; González Otero,Alejandro; Carnot Uría,José; Fernández Ramos,Oscar; Hernández Ramírez,Porfirio;
Revista Cubana de Hematolog?-a, Inmunolog?-a y Hemoterapia , 2000,
Abstract: acute promyelocytic leukemia (apl) is characterized by the reciprocal translocation t(15; 17) that results in fusion gene pml-rara formation. as apl is considered to be an hematological emergency and also is given a very specific treatment with retinoic acid (atra), then it is very important to diagnose quickly and accurately which makes it possible in many cases to save the patient's life. at present rt- pcr methods have been developed to detect pml-rara gen. these molecular techniques have been extremely useful in diagnosing this entity. this paper sets forth the preliminary results of molecular diagnoses of apl in 38 patients. 36 cases presented the fusion gene and 2 did not. of the total number of positive patients, 19(55%) were bcr 1, 2 (5%) were bcr 2 and 14(40%) bcr 3. all the patients with positive results were responsive to treatment with atra. one of the two patients with negative results showed the existence of t (11; 17). these two patients did not respond to treatment with atra
On the Consistency of the First-Order-Approach to Principal-Agent Problems  [PDF]
óscar Gutiérrez
Theoretical Economics Letters (TEL) , 2012, DOI: 10.4236/tel.2012.22028
Abstract: This paper revisits the principal-agent model with moral hazard when its solution is obtained invoking the first-order-approach. We show that the solution can be economically inconsistent even when “sufficient conditions” ensuring its validity ([1,2]) hold. To be more precise, we provide examples where is impossible to find Lagrange multipliers validating the approach. The correct solution to the problem provides a rationale for option-like contracts and minimum payments.
Impact Assessment of Tourism Construction in Cuba  [PDF]
Lourdes Ruiz Gutiérrez
Journal of Building Construction and Planning Research (JBCPR) , 2015, DOI: 10.4236/jbcpr.2015.31002
Abstract: This paper discusses a methodology for the environmental assessment of tourist facilities located in Cuban coastal zones in which the general aspects of the process are discussed, defined, and characterized, and examines the main processes that occur as a result of the negative impacts of construction activities. It establishes the theoretical conceptual framework underlying the research and includes a detailed analysis and processing of a sample of tourism projects located in coastal ecosystems. The methodology is complemented by results that include proposed strategic lines of action and a plan for managing negative environmental impacts throughout the life cycle of tourist facilities through prevention, correction and mitigation measures. It ends with conclusions and recommendations to guide tourism development toward environmental sustainability.
The Environmental Effects of Tourism Architecture on Island Ecosystem in Cayo Guillermo, Cuba  [PDF]
Lourdes Ruiz Gutiérrez
Journal of Environmental Protection (JEP) , 2015, DOI: 10.4236/jep.2015.69093
Abstract: The objective of this study is to determine the main adverse impacts that may be caused by tourism architecture upon island ecosystems consisting of small islands or islets and to propose an architectural and landscape design with a focus on the environmental sustainability of the same. A study of projects was undertaken in Cayo Guillermo, located to the north of Ciego de Avila in Cuba using methods of life-cycle assessment of buildings, matrix methods of the activities that caused severe environmental impact and statistical processing through multivariate analysis. Conclusions were reached on the need for designs that did not harm the ecosystems of islands with high ecological fragility. It has been determined that suitable construction intervention would decrease the negative impact and would allow the natural resources of these valuable ecosystems that are the basis for responsible sun and sand tourism to be preserved.
Page 1 /6352
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.