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Search Results: 1 - 10 of 12743 matches for " Nilton Garcia Marengoni "
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Extraction of genomic DNA from solid tissues of teleostei fish/ Extra o de DNA gen mico em tecidos sólidos de peixes teleósteos
Nilton Garcia Marengoni,Marcia Regina Fragoso Machado,Eliane Gasparino
Semina : Ciências Agrárias , 2006,
Abstract: The object of this work was to extract the genomic DNA of solid tissue from Nile tilapia (Oreochromis niloticus), pacu (Piaractus mesopotamicus), piau (Leporinus sp.) and curimba (Prochilodus lineatus), using the methods Proteinase K (PK) and Cetyltrimethylammiun Bromide (CTAB). The DNA extractedfrom samples of liver, kidney, tail fin, heart, muscle and gills were quantified in spectrophotometer to determine the concentration and purity through the ratio A260nm/A280nm. The data were statistically analyzed and there were no significant effect of interaction between species and tissues about the purityand concentration of the DNA obtained with CTAB, but for the PK there were interaction about the concentration. Using the CTAB method was verified that the mean quantity of DNA in the curimbakidney was significantly (p<0.05) lower (106.98 μg/mL) than the value observed in pacu, whereas, did not differ from the variables found in piau (497.20 μg/mL) and tilapia (234.50 μg/mL). For the PK method, the mean quantity of DNA using the muscle of Nile tilapia presented the lowest value (117.35 μg/mL) with preference to the other tissues and analyzed species (P<0.05). The ratio A260nm/A280nm changed from 1.7 to 2.0 and 1.6 to 2.1 for the PK and CTAB methods, respectively. It may be concluded that the DNA purity was satisfactory for the different tissues on the four species of fish. O objetivo desse trabalho foi extrair o DNA gen mico de tecidos sólidos de tilápia do Nilo (Oreochromis niloticus), pacu (Piaractus mesopotamicus), piau (Leporinus sp.) e curimba (Prochilodus lineatus), utilizando os métodos Proteinase K (PK) e Brometo de Cetiltrimetilam nio (CTAB). O DNA extraído das amostras de fígado, rim, nadadeira caudal, cora o, músculo e branquias foi quantificado em espectrofot metro para determina o da concentra o e da pureza por meio da raz o A260nm/A280nm. Os dados foram estatisticamente analisados e n o se observou efeito significativo para a intera o entre espécies e tecidos em rela o à pureza e concentra o do DNA obtido a partir do CTAB, porém para PK houve intera o em rela o à concentra o. Utilizando o método CTAB verificou-se que a concentra o média de DNA no rim de curimba foi significativamente (p < 0,05) inferior (106,98 ?g/mL) à observada em pacu (1727,90 ?g/mL), porém n o diferiram das encontradas em piau (497,20 ?g/mL) e tilápia (234,50 ?g/ mL). Para o método PK, a concentra o média de DNA utilizando o músculo de tilápia do Nilo apresentou o menor valor (117,35 ?g/mL) em rela o aos demais tecidos e espécies analisadas (p < 0,05). A raz o A
Centesimal, microbiological, and sensory characterization of fishburgers made with mechanically separated meat of tilapia Caracteriza o microbiológica, sensorial e centesimal de fishburgers de carne de tilápia mecanicamente separada
Nilton Garcia Marengoni,Magali Soares dos Santos Pozza,Gilberto Costa Braga,Douglas Batista Lazzeri
Revista Brasileira de Saúde e Produ??o Animal , 2009,
Abstract: This work aimed to evaluate the microbiological, sensory and centesimal characterization of four kinds of fishburgers elaborated with tilapia mechanically separated meat. The microbiological analyses showed that both raw material and fishburgers met the microbiological standards for products derived from fish and certified that the use of byproducts derived from fish processing is a form of aggregating value to a final product, with good acceptance by the consumer, assisting quality standards. It was not verified significant differences for the averages attributed for taste, aroma, softness, global appearance and purchase intention that ranged, respectively, from 7.14 to 7.44; 7.14 to 7.46; 7.30 to 7.42; 7.21 to 7.35; 3.86 to 3.98. All fish burgers were moderately and very accepted and did not have a preferred specific one. The results of purchase intention oscillated between “maybe would buy”/”wouldn’t buy” and “possibly would buy the product”. The physical-chemical analysis presented the following results: 15.50 to 16.14; 1.73 to 10.28 and 1.12 to 2.44 g/100 g for crude protein, ethereal extract and mineral matter, respectively. The unit cost ranged from R$ 0.36 to R$ 0.37 with yield of 12 units/kg, that denote the production viability. Objetivou-se com este trabalho avaliar as características microbiológicas, sensorial e centesimal de quatro tipos de fishburgers elaborados à base de carne de tilápia mecanicamente separada (CMS). As análises microbiológicas demonstraram que tanto a matéria-prima quanto os fishburgers atenderam aos padr es microbiológicos para derivados de pescado, constatando-se que a utiliza o de subprodutos de processamento do pescado é uma forma de agregar valor a um produto final, com boa aceita o pelo consumidor e atendendo aos padr es de qualidade. N o foram observadas diferen as significativas para as médias atribuídas aos parametros sabor, aroma, maciez, aparência global e inten o de compra, que oscilaram, respectivamente, entre 7,14 e 7,44; 7,14 e 7,46; 7,30 e 7,42; 7,21 e 7,35; 3,86 e 3,98. Todos os fishburgers estiveram entre moderadamente e muito aceitos, n o havendo preferência por um específico. Os resultados referentes à inten o de compra variaram entre “talvez comprasse”/”n o comprasse” e “possivelmente compraria o produto”. A análise físico-química indicou resultados de 15,50 a 16,14; 1,73 a 10,28 e 1,12 a 2,44 g/100 g para proteína bruta, extrato etéreo e matéria mineral, respectivamente. O custo unitário variou de R$0,36 a R$ 0,37, com rendimento de 12 unidades/kg, o que denota a viabilidade da produ o.
Estado trófico e bioacumula o do fósforo total no cultivo de peixes em tanques-rede na área aquícola do reservatório de Itaipu = Trophic level and bioaccumulation of total phosphorus in cage fish rearing in the aquaculture area at Itaipu reservoir
Guilherme Wolff Bueno,Nilton Garcia Marengoni,Affonso Celso Gon?alves Júnior,Wilson Rogério Boscolo
Acta Scientiarum : Biological Sciences , 2008,
Abstract: A intensa produ o de peixes e utiliza o dos recursos hídricos podecomprometer a qualidade e disponibilidade da água, além de afetar a capacidade de suporte do ambiente, causando eutrofiza o e, consequentemente, a polui o do mesmo. Realizouse um estudo das variáveis físico-químicas da água e dos teores de fósforo total (P-total) no sedimento, ra es e peixes cultivados em tanques-rede em uma área aquícola localizada no reservatório da Itaipu, no município de Santa Helena, Estado do Paraná, objetivando monitorar a qualidade da água e determinar o índice de eutrofiza o total da área de cultivo, além de analisar a bioacumula o de P-total nos peixes e sedimento. As variáveis físicoquímicas da água mantiveram valores normais indicados pelo Conama. A concentra o média de P-total na área aquícola foi de 28,41 IET e com transparência por disco de Secchide 2,74 m, enquadrando o ambiente como oligotrófico. Houve correla o de 0,55 (p < 0,01) entre estas duas variáveis. A bioacumula o de P-total nos sedimentos foi diminuindo de 0,76 a 0,35 g kg-1 ao longo do cultivo. Os peixes acumularam, em média, 0,30 a 2,82 g kg-1. Esta atividade pode ser utilizada para biomanipula o do fósforo total emreservatórios similares. The intense production of fishes and the use of water resources can compromise the quality and availability of water, in addition to its effects on environmental carrying capacity, causing eutrophication andconsequently water pollution. Therefore, the objective of this study was to monitor the physical-chemical water variables and to measure the total phosphorus (total-P) in sediment, feed and fish in cage culture in an aquaculture area situated at the Itaipu reservoirin Santa Helena – Paraná State, observing water quality and determining the total eutrophic level on the aquaculture area, in addition to analyzing total-P bioaccumulation in fish andsediment. The physical-chemical water variables presented normal values as indicated by Conama. The mean concentration of total-P for the aquaculture area was 28.41 IET and Secchi disc transparency of 2.74 m, giving it an oligotrophic classification. There was acorrelation of 0.55 (p < 0.01) between these two variables. The bioaccumulation of total-P in sediments decreased from 0.76 to 0.35 g kg-1 during this period. Mean fish accumulation ranged from 0.30 to 2.82 g kg-1. This activity can be used in the biomanipulation of total-P in similar reservoirs.
Efeitos de floculantes na concentra o de micro e macronutrientes em biofertilizante suíno = The effect of flocculants on the concentrations of micro and macronutrients in swine biofertilizer
Affonso Celso Gon?alves Júnior,Cleber Ant?nio Lindino,Ossival Lolato Ribeiro,Nilton Garcia Marengoni
Acta Scientiarum : Technology , 2008,
Abstract: O objetivo deste trabalho foi avaliar quimicamente as fases sólida e líquida de biofertilizante suíno, separadas com diferentes floculantes. O delineamento experimental foi inteiramente casualizado, com cinco tipos de floculantes, sendo cinco doses de cadafloculante, com cinco repeti es. A separa o foi efetuada por meio deflocula o/decanta o por 1h após a adi o do floculante. Realizou-se a caracteriza o química das fases sólida e líquida do biofertilizante, a partir da determina o de N, P, K, Ca, Mg, Cu, Zn, Fe e Mn. Na fase líquida, o objetivo de diminuir os macronutrientes foi atingido quando se utilizaram as doses 10 e 20 mL da combina o entre calcário dolomítico + hidróxido de cálcio, e os micronutrientes foram reduzidos quando foram utilizados os floculantes sulfato de alumínio e hidróxido de cálcio + sulfato de alumínio. The objective of this work was to chemically evaluate the solid liquid phases of swine biofertilizer separated by different flocculants. The experimental design was completely randomized, with five kinds of flocculants and five doses of each flocculant, with five repetitions. The separation was conducted through flocculation/decantation for an hour after the addition of each flocculent. The chemical characterization was done on the solid and liquid biofertilizer phases from the determination of the following elements: N, P, K, Ca, Mg, Cu, Zn, Fe and Mn. For the liquid phase, the purpose of decreasing macronutrients was achieved using the doses of 10 and 20 mL of the combination between the flocculants dolomitic limestone + calcium hydroxide, and micronutrients reduction occurred when the flocculants aluminum sulfate and calcium hydroxide + aluminum sulfate were used.
Produtividade e componentes de produ??o da soja adubada com diferentes doses de fósforo, potássio e zinco
Gon?alves Júnior, Affonso Celso;Nacke, Herbert;Marengoni, Nilton Garcia;Carvalho, Endrigo Ant?nio de;Coelho, Gustavo Ferreira;
Ciência e Agrotecnologia , 2010, DOI: 10.1590/S1413-70542010000300019
Abstract: the correct productivity increment must be allied to the technological advance in the knowledge of the nutritional requirements of each culture, thus allowing for the correct use of the inputs in the agricultural property. with the objective of studying the plants of soybean reply to zinc (zn) in relation to the different doses of phosphorus (p) and potassium (k), this experiment was performed in the municipality of palotina-pr. the soil was classified as typic hapludalf. the treatments were arranged in a 3x3 factorial scheme, constituting three fertilization forms with p2o5 e k2o: without fertilization, recommended dose and twice the recommended fertilization dose by the soil analysis and three zn doses (0; 2 and 4 kg ha-1). nutrient sources used were bicalcic phosphate for p, potassium chloride for k and zinc sulphate for zn. the applied zn doses did not significantly influence productivity, so supplementing the base fertilization form is not justified. on other hand, fertilization with p2o5 e k2o resulted in a significant increase in productivity and an increase in the number of legumes per plant, with an increase of the doses of these nutrients in the soil.
Produtividade do milho em resposta a aduba??o com NPK e Zn em argissolo vermelho-amarelo eutrófico e latossolo vermelho eutroférrico
Gon?alves Júnior, Affonso Celso;Trautmann, Ricardo Robson;Marengoni, Nilton Garcia;Ribeiro, Ossival Lolato;Santos, Alexandre Leseur dos;
Ciência e Agrotecnologia , 2007, DOI: 10.1590/S1413-70542007000400043
Abstract: it's with the purpose of evaluating the production components and the corn crop productivity due fertilization with different doses of zn (zinc) an npk (nitrogen, phosphorus and potassium), this research has been done over the municipalities of palotina-pr, on eutrophic red-yellow argissol and marechal candido rondon - pr, on red eutroferric latossol. the treatments were arranged on a factorial scheme 3x3 constituting three doses of fertilization npk: no fertilization, once (30-100-50 kg ha-1) and twice the fertilization recommended (60-200-100 kg ha-1), based on the soil analysis. another factor under studying was the zn in different doses: 0,0; 5,0 and 10,0 mg dm-3. such parcels were corresponded to 3,6 m width by 6 m length. it were sowed 4 spaced lines with 0,9 m, using as a useful parcel the 2 central lines discarding 1,5 m of edging. the plant population was about 6,5 seeds by linear meter. considering the npk fertilization it was gotten a considerably increase for the components of seeds number, 1000 seeds mass and the productivity, being the dose 60 - 200 - 100 kg ha-1 the one that has given the best results on red eutroferric latossol and on eutrophic red-yellow argissol that has given a considerably increase on the 1000 seeds and productivity. according to the fertilization with zn, it has not given a considerably increase on both soils among the treatments for the evaluated components and productivity. with this word it can be concluded that the fertilization with npk increased the crop productivity on both soils while the fertilization with zn differences were not gotten over corn crop productivity.
Productive performance and economic viability the juvenile of the Nile tilapia cultivated in the west of Paraná submitted to different stocking densities Desempenho produtivo e viabilidade econ mica de juvenis de tilápia-do-Nilo cultivados na regi o oeste do Paraná sob diferentes densidades de estocagem
Nilton Garcia Marengoni,Guilherme Wolff Bueno,Affonso Celso Gon?alves Júnior,Ana Alix Mendes de Almeida Oliveira
Revista Brasileira de Saúde e Produ??o Animal , 2008,
Abstract: It was aimed at with this work to evaluate the effect of the stocking density on the performance parameters, survival and economic viability of the production of juvenile of Nile tilapia (Oreochromis niloticus), Chitralada strain in half-intensive system, using twelve tank nurseries. 2700 juvenile sexually reverted with initial average weight of 0.65 g were distributed in an entirely randomized experimental design with four treatments (different stocking densities: 1, 2, 3, 4 peixes/m3) and three repetitions. During the tracking of the physical-chemical variables of the water, the pH (6.6 to 7.1) and the conductivity (24 μS/cm) had presented satisfactory average values. However, the transparency (47 to 87 cm), the dissolved oxygen (3.09 to 4.60 mg/L) and the temperature (19 to 24oC) had been outside of the normal standards recommended for the tropical fish culture. At the of the 84 days of culture the average daily mean weight (0.61 to 1.02 g/day) and the specific growth (5.22 to 5.82%) of the juveniles had presented a negative linear relationship (p<0.05) and the alimentary conversion (0.98 to 1.84) a quadratic relationship (p<0.01) in function to the stocking density. It was concluded that, in the conditions where these studies had been carried through, the stocking densities had influenced in the productive performance the juvenile of Nile tilapia, providing an increase in the ration consumption and consequently in the production cost. Through an economical analysis of the production the best economical viability is verified when used a density of up to 3 fish/m3. Objetivou-se com este trabalho avaliar o efeito da densidade de estocagem, sobre os parametros de desempenho e sobrevivência, e a viabilidade econ mica da produ o de juvenis de tilápia-do-Nilo (Oreochromis niloticus), linhagem Chitralada, em sistema semi-intensivo, utilizando-se doze viveiros escavados. 2700 juvenis sexualmente revertidos com peso médio inicial de 0,65 g foram distribuídos em um delineamento inteiramente casualizado com quatro tratamentos (diferentes densidades de estocagem: 1, 2, 3, 4 peixes/m3) e três repeti es. Durante o monitoramento das variáveis físico-químicas da água, o pH (6,6 a 7,1) e a condutividade (24 μS/cm) apresentaram valores médios satisfatórios. Porém, a transparência (47 a 87 cm), o oxigênio dissolvido (3,09 a 4,60 mg/L) e a temperatura (19 a 24oC) ficaram fora dos padr es normais recomendados para o cultivo de peixes tropicais. Ao final dos 84 dias de cultivo, o ganho de peso médio diário (0,61 a 1,02 g/dia) e o crescimento específico (5,22 a 5,82%) do
Performance e reten o de metais pesados em três linhagens de juvenis de tilápia-do-Nilo em hapas = Performance and retention of heavy metals in fingerlings of Nile tilapia in hapas
Nilton Garcia Marengoni,Maikel Possamai,Affonso Celso Gon?alves Júnior,Ana Alix Mendes de Almeida Oliveira
Acta Scientiarum : Animal Sciences , 2008,
Abstract: O trabalho objetivou avaliar o desempenho produtivo de três linhagens de tilápia-do-Nilo (Oreochromis niloticus), GIFT, Bouaké e Chitralada, durante a produ o de juvenis no período de fevereiro a maio de 2007. Os alevinos sexualmente revertidos foram alojados em 12 hapas com 9 m3, instaladas em um tanque escavado com 0,7 ha de lamina de água. Ao final de 93 dias de cultivo, verificou-se que houve diferen a significativa para a sobrevivência entre as três linhagens (p < 0,05), em que a GIFT (96,80%) superou a Chitralada (90,85%) e Bouaké (77,74%). O peso médio final (40,49, 34,53 e 32,42 g), ganhode peso diário (0,43, 0,37 e 0,34 g dia-1), convers o alimentar (1,80, 1,81 e 1,81) e biomassa final (19.594,79, 19.528,85 e 16.488,99 g) n o apresentaram diferen as significativas entre aGIFT, Bouaké e Chitralada, respectivamente. A média dos peixes com peso acima de 25 g superou 80% dos lotes, garantindo uniformidade e padroniza o dos juvenis. Nos peixes, os metais tóxicos cádmio e chumbo ficaram acima do limite tolerável, porém o metal cromon o foi detectado. A linhagem GIFT apresenta a melhor performance apenas em rela o à sobrevivência, neste sistema de cultivo, porém com maior acúmulo de metais pesados em compara o às outras duas linhagens. This assay aimed to evaluate the productive performance of three Niletilapia (Oreochromis niloticus) strains – GIFT (Genetic Improvement of Farmed Tilapia), Bouake and Chitralada – during the production of juveniles from February to May, 2007. The sexually reverted fingerlings were stored in twelve 9 m3 hapa nets installed in a pondwith 0.7 ha water blade. At the end of 93 days of culture, a significant change in survival among the three strains (p < 0.05) was verified, where GIFT (96.80%) surpassed Chitralada (90.85%) and Bouake (77.74%). The average final weight (40.49, 34.53 and 32.42 g), daily body mass gain (0.43, 0.37 and 0.34 g day-1) feed conversion (1.80; 1.81 and 1.81) and final biomass (19,594.79, 19,528.85 and 16,488.99 g), did not present significant differences among GIFT, Bouake and Chitralada, respectively. The average of fish over 25 g was over 80% of the lots, which guaranteed uniformity and standardization of the juveniles. The toxic metals cadmium and lead were above the tolerable level in the fish; however, the metal chromium was not detected. The GIFT strain presented the best performance only for the survival rate in this culture system, although it contained high indices of heavy metals in comparison with the other two strains.
Evaluation of the performance of two strains of Nile tilapia (Oreochromis Niloticus) in mixed raising systems
Neves, Patrícia Ribeiro;Ribeiro, Ricardo Pereira;Vargas, Lauro;Natali, Maria Raquel Mar?al;Maehana, Káttia Regina;Marengoni, Nilton Garcia;
Brazilian Archives of Biology and Technology , 2008, DOI: 10.1590/S1516-89132008000300012
Abstract: the aim of this study was to evaluate the productive performance of two strains of nile tilapia (oreochromis niloticus) in mixed raising systems. a total of 3600 fish-larvae species was used, 1800 belonging to bouaké lineage, and 1800 to chitralada. the experiment was carried out in three phases; phase i in an incubator in 18 boxes, in which two treatments (bouaké and chitralada) were tested by using nine repetitions; phases ii and iii were performed in 18 cement tanks with the same treatments. in phase i, regarding the final weight and gain of weight, chitralada strain showed the highest final weight values. in phase ii, chitralada showed the highest final weight value when compared with bouaké, and, considering the gain of weight, bouaké obtained the best result. in phase iii, chitralada showed better final weight results (104 days of raising), final weight, final length and gain of length/cm (152 days of raising); but, after 279 days of the cultivation, bouaké showed a higher weight and length gain. these findings showed that chitralada strain presented the best performance.
Influência da espessura do osso cortical sobre a velocidade de propaga??o do ultrassom
Mandarano-Filho, Luiz Garcia;Bezuti, Márcio Takey;Mazzer, Nilton;Barbieri, Cláudio Henrique;
Acta Ortopédica Brasileira , 2012, DOI: 10.1590/S1413-78522012000300010
Abstract: objective: an experimental in vitro study was carried out to evaluate the influence of cortical bone thickness on ultrasound propagation velocity. methods: sixty bone plates were used, made from bovine femurs, with thickness ranging from 1 to 6 mm (10 of each). the ultrasound velocity measurements were performed using a device specially designed for this purpose, in an underwater acoustic tank and with direct contact using contact gel. the transducers were positioned in two ways: on opposite sides, with the bone between them, for the transverse measurement; and parallel to each other, on the same side of the bone plates, for the axial measurements. results: in the axial transmission mode, the ultrasound velocity speed increased with cortical bone thickness, regardless of the distance between the transducers, up to a thickness of 5 mm, then remained constant thereafter. there were no changes in velocity when the transverse measures were made. conclusion: ultrasound velocity increased with cortical bone thickness in the axial transmission mode, until the thickness surpasses the wavelength, after which point it remained constant. level of evidence: experimental study.
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