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6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy
Stojiljkovic Maja,Fazlagic Amira,Dokmanovic-Krivokapic Lidija,Nikcevic Gordana
Human Genomics , 2012, DOI: 10.1186/1479-7364-6-19
Abstract: The Golden Helix Pharmacogenomics Days are international scientific meetings aiming to educate healthcare professionals and biomedical scientists about pharmacogenomics and personalized medicine. In this meeting report, we provide an overview of the scientific lectures and the topics discussed during the 6th Golden Helix Pharmacogenomics Day that was held in Belgrade, Serbia last June 5, 2012. The scientific program included lectures by the local and international speakers from Europe and the United States.
Purification and functional analysis of the recombinant protein isolated from E. coli by employing three different methods of bacterial lysis
Journal of the Serbian Chemical Society , 2005,
Abstract: In this paper, the purification of the human recombinant protein expressed in E. coli using the GSTGene Fusion System, by applying various methods of bacterial lysis: sonication, freeze/thaw and beadbeating, is presented. The study was an attempt to compare the properties of the proteins obtained by the sonication method, recommended by manufacturers but inaccessible for many researchers, with those obtained using two other readily available lysis methods. The data show that all purified proteins were soluble and intact with the highest protein yield being obtained via the freeze/thaw method. The results of functional analysis indicate that the proteins purified using the sonication and freeze/thaw methods of lysis exhibited similar DNA binding affinity, while the protein purified by beadbeating was also functional but with a lower binding affinity. The conclusion of this study is that all three lysis methods could be successfully employed for protein purification.
Complete curvature homogeneous pseudo-Riemannian manifolds
P. Gilkey,S. Nikcevic
Physics , 2004, DOI: 10.1088/0264-9381/21/15/009
Abstract: We exhibit 3 families of complete curvature homogeneous pseudo-Riemannian manifolds which are modeled on irreducible symmetric spaces and which are not locally homogeneous. All of the manifolds have nilpotent Jacobi operators; some of the manifolds are, in addition, Jordan Osserman and Jordan Ivanov-Petrova.
Curvature homogeneous spacelike Jordan Osserman pseudo-Riemannian manifolds
P. Gilkey,S. Nikcevic
Physics , 2003, DOI: 10.1088/0264-9381/21/2/013
Abstract: Let s be at least 2. We construct Ricci flat pseudo-Riemannian manifolds of signature (2s,s) which are not locally homogeneous but whose curvature tensors never the less exhibit a number of important symmetry properties. They are curvature homogeneous; their curvature tensor is modeled on that of a local symmetric space. They are spacelike Jordan Osserman with a Jacobi operator which is nilpotent of order 3; they are not timelike Jordan Osserman. They are k-spacelike higher order Jordan Osserman for $2\le k\le s$; they are k-timelike higher order Jordan Osserman for $s+2\le k\le 2s$, and they are not k timelike higher order Jordan Osserman for $2\le s\le s+1$.
Pseudo-Riemannian Jacobi-Videv Manifolds
P. Gilkey,S. Nikcevic
Mathematics , 2007, DOI: 10.1142/S0219887807002272
Abstract: We exhibit several families of Jacobi-Videv pseudo-Riemannian manifolds which are not Einstein. We also exhibit Jacobi-Videv algebraic curvature tensors where the Ricci operator defines an almost complex structure.
The classification of simple Jacobi--Ricci commuting algebraic curvature tensors
P. Gilkey,S. Nikcevic
Mathematics , 2007,
Abstract: We classify algebraic curvature tensors such that the Ricci operator is simple (i.e. the Ricci operator is complex diagonalizable and either the complex spectrum consists of a single real eigenvalue or the complex spectrum consists of a pair of eigenvalues which are complex conjugates of each other) and which are Jacobi--Ricci commuting (i.e. the Ricci operator commutes with the Jacobi operator of any vector).
Kaehler and para-Kaehler curvature Weyl manifolds
Peter Gilkey,Stana Nikcevic
Mathematics , 2010,
Abstract: We show that the Weyl structure of an almost-Hermitian Weyl manifold of dimension at least 6 is trivial if the associated curvature operator satisfies the Kaehler identity. Similarly if the curvature of an almost para-Hermitian Weyl manifold of dimension at least 6 satisfies the para-Kaehler identity, then the Weyl structure is trivial as well.
4-dimensional (para)-K?hler--Weyl structures
Peter Gilkey,Stana Nikcevic
Mathematics , 2012,
Abstract: We give an elementary proof of the fact that any 4-dimensional para-Hermitian manifold admits a unique para-Kaehler--Weyl structure. We then use analytic continuation to pass from the para-complex to the complex setting and thereby show any 4-dimensional pseudo-Hermitian manifold also admits a unique Kaehler--Weyl structure.
Affine projective Osserman structures
Peter Gilkey,Stana Nikcevic
Mathematics , 2013, DOI: 10.1088/0264-9381/30/15/155015
Abstract: By considering the projectivized spectrum of the Jacobi operator, we introduce the concept of projective Osserman manifold in both the affine and in the pseudo-Riemannian settings. If M is an affine projective Osserman manifold, then the modified Riemannian extension metric on the cotangent bundle is both spacelike and timelike projective Osserman. Since any rank 1 symmetric space is affine projective Osserman, this provides additional information concerning the cotangent bundle of a rank 1 Riemannian symmetric space with the modified Riemannian extension metric. We construct other examples of affine projective Osserman manifolds where the Ricci tensor is not symmetric and thus the connection is not the Levi-Civita connection of any metric. If M is an affine projective Osserman manifold of odd dimension, we use methods of algebraic topology to show the Jacobi operator has only one non-zero eigenvalue and that eigenvalue is real.
Nilpotent Spacelike Jorden Osserman pseudo-Riemannian manifolds
P. Gilkey,S. Nikcevic
Mathematics , 2003,
Abstract: Pseudo-Riemannian manifolds of balanced signature which are both spacelike and timelike Jordan Osserman nilpotent of order 2 and of order 3 have been constructed previously. In this short note, we shall construct pseudo-Riemannian manifolds of signature (2s,s) for any s (which is at least 2) which are spacelike Jordan Osserman nilpotent of order 3 but which are not timelike Jordan Osserman. Our example and techniques are quite different from known previously both in that they are not in neutral signature and that the manifolds constructed will be spacelike but not timelike Jordan Osserman.
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