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Search Results: 1 - 10 of 298459 matches for " Niels Terp Kjeldgaard J?rgensen "
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QoS Self-Provisioning and Interference Management for Co-Channel Deployed 3G Femtocells
Troels Kolding,Pawel Ochal,Niels Terp Kjeldgaard Jrgensen,Klaus Pedersen
Future Internet , 2013, DOI: 10.3390/fi5020168
Abstract: A highly efficient self-provisioning interference management scheme is derived for 3G Home Node-Bs (HNB). The proposed scheme comprises self-adjustment of the HNB transmission parameters to meet the targeted QoS (quality of service) requirements in terms of downlink and uplink guaranteed minimum throughput and coverage. This objective is achieved by means of an autonomous HNB solution, where the transmit power of pilot and data are adjusted separately, while also controlling the uplink interference pollution towards the macro-layer. The proposed scheme is evaluated by means of extensive system level simulations and the results show significant performance improvements in terms of user throughput outage probability, power efficiency, femtocell coverage, and impact on macro-layer performance as compared to prior art baseline techniques. The paper is concluded by also showing corresponding measurements from live 3G high-speed packet access (HSPA) HNB field-trials, confirming the validity of major simulation results and assumptions.
Collaborating with Young Adults Diagnosed with Schizophrenia: A Participatory Design Study to Shape the Healthcare System  [PDF]
Malene Terp, Charlotte D. Bj?rnes, Rikke Jrgensen, Jan Mainz, Birgitte Schantz Laursen
Open Journal of Nursing (OJN) , 2017, DOI: 10.4236/ojn.2017.77056
Abstract: Introduction: Disengagement from mental health services in young adults with schizophrenia has been associated with dissatisfaction and unmet needs. Striving to improve engagement, we invited service users recently diagnosed with schizophrenia to be co-designers of a smartphone technology that will be responsive to their needs. Aim: This paper reports the first phase of a three-phased participatory design process. The objective was to identify needs of support in young adults recently diagnosed with schizophrenia and to generate ideas of how the needs could be accommodated using smartphone technology. Methods: Participatory design guided the research process and a qualitative approach was used to generate and analyse the data. Data were generated by means of participant observations (n = 45 hours) and interviews (n = 6) with young adults from a first episode psychosis program in Denmark. Findings: Low levels of knowledge and high levels of uncertainties are characteristic of young adults recently diagnosed with schizophrenia, bringing about a vast need of support in order for them to gain power over their new life situation. Our study suggests that the smartphone may be used to foster empowerment by guiding the young adult’s actions in situ, providing comprehensive and easily understood information on the go, allowing for recovery tracking, and notification of mental health changes, providing medication overview and giving easy access to healthcare providers. Conclusion: Young adults recently diagnosed with schizophrenia require comprehensive support in order to become empowered to confidently manage their new life situation. The smartphone holds this potential by offering flexible collaboration and timely access to self-management resources
Functional characteristics of culturable bacterioplankton from marine and estuarine environments
Frette,Lone; Johnsen,Kaare; Jrgensen,Niels O. G.; Nybroe,Ole; Kroer,Niels;
International Microbiology , 2004,
Abstract: information on the structure of bacterioplankton communities is continuously increasing, while knowledge of their metabolic capabilities remains limited. in this study, the metabolic capacity of bacterioplankton was investigated, as such information is necessary to fully understand carbon cycling and other biogeochemical processes. the diversity of dominant culturable chemoorganotrophic bacteria from one estuarine and three marine environments was analyzed by random isolation of colony-forming units on solid media, taxonomical identification by partial 16s rrna gene sequence analysis, and functional characterization of the isolates. a total of 76 16s rrna gene sequences, representing 19 different genotypes, were obtained from the four sampling localities, including bacillus, pseudomonas, pseudoalteromonas, vibrio, and erythrobacter as the most frequently isolated genera. the range of metabolic functions possessed by the cultured bacterial assemblages differed significantly between sites. similarly, the percentage at each sampling station of bacteria capable of performing a specific function was significantly different for 18 of the 25 investigated metabolic functions. at two localities, the bacterial assemblages were dominated by a single genus (pseudoalteromonas or erythrobacter) and appeared to be functionally specialized. more than 95% of the isolates were capable of utilizing dissolved free amino acids and protein as their sole nitrogen sources, and all isolates of the specialized assemblages expressed β-glucosidase. furthermore, only some of the isolates were able to utilize nh4+, while up to two thirds of the isolates of the two marine sites were able to grow on no3-.
A Phosphorylation Tag for Uranyl Mediated Protein Purification and Photo Assisted Tag Removal
Qiang Zhang, Thomas J. D. Jrgensen, Peter E. Nielsen, Niels Erik M?llegaard
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0091138
Abstract: Most protein purification procedures include an affinity tag fused to either the N or C-terminal end of the protein of interest as well as a procedure for tag removal. Tag removal is not straightforward and especially tag removal from the C-terminal end is a challenge due to the characteristics of enzymes available for this purpose. In the present study, we demonstrate the utility of the divalent uranyl ion in a new procedure for protein purification and tag removal. By employment of a GFP (green florescence protein) recombinant protein we show that uranyl binding to a phosphorylated C-terminal tag enables target protein purification from an E. coli extract by immobilized uranyl affinity chromatography. Subsequently, the tag can be efficiently removed by UV-irradiation assisted uranyl photocleavage. We therefore suggest that the divalent uranyl ion (UO22+) may provide a dual function in protein purification and subsequent C-terminal tag removal procedures.
Estimation of health effects of prenatal methylmercury exposure using structural equation models
Esben Budtz-Jrgensen, Niels Keiding, Philippe Grandjean, Pal Weihe
Environmental Health , 2002, DOI: 10.1186/1476-069x-1-2
Abstract: Structural equation models were developed for assessment of the association between biomarkers of prenatal mercury exposure and neuropsychological test scores in 7 year old children. Eleven neurobehavioral outcomes were grouped into motor function and verbally mediated function. Adjustment for local dependence and item bias was necessary for a satisfactory fit of the model, but had little impact on the estimated mercury effects. The mercury effect on the two latent neurobehavioral functions was similar to the strongest effects seen for individual test scores of motor function and verbal skills. Adjustment for contaminant exposure to poly chlorinated biphenyls (PCBs) changed the estimates only marginally, but the mercury effect could be reduced to non-significance by assuming a large measurement error for the PCB biomarker.The structural equation analysis allows correction for measurement error in exposure variables, incorporation of multiple outcomes and incomplete cases. This approach therefore deserves to be applied more frequently in the analysis of complex epidemiological data sets.Observational studies in epidemiology often involve several sources of uncertainty, such as measurement error, proxy variables of unknown validity, confounder adjustment, and multiple comparisons with outcome variables. Standard statistical methods are poorly suited to deal with these problems, especially when all of them occur at the same time. During the past decade or so, the technique of structural equation analysis has been advanced and made more easily available through software packages. Studies in environmental epidemiology have started to incorporate this approach [1,2], although most studies have focused on estimating the relative importance of exposure sources, e.g., with regard to lead concentrations in the body [3]. New user-friendly software offers opportunities that we have explored in a complex data set from an environmental epidemiology study on the effects of develop
UGT2B17 Genotype and the Pharmacokinetic Serum Profile of Testosterone during Substitution Therapy with Testosterone Undecanoate. A Retrospective Experience from 207 Men with Hypogonadism
Anne Kirstine Bang,Niels Jrgensen,Ewa Rajpert-De Meyts,Anders Juul
Frontiers in Endocrinology , 2013, DOI: 10.3389/fendo.2013.00094
Abstract: Background: Testosterone (T) is mainly excreted in the urine as testosterone glucuronide (TG). This glucuronidation is partly dependent on the UGT2B17 genotype, and TG excretion is therefore lower in men having the UGT2B17 deletion. However, the possible influence of UGT2B17 genotype on serum T during androgen therapy is unknown. We retrospectively investigated the possible association between the UGT2B17 gene polymorphism and serum T levels in hypogonadal men during Testosterone undecanoate (TU) substitution therapy.
Evaluation of Functional Erythropoietin Receptor Status in Skeletal Muscle In Vivo: Acute and Prolonged Studies in Healthy Human Subjects
Britt Christensen, Carsten Lundby, Niels Jessen, Thomas S. Nielsen, Poul F. Vestergaard, Niels M?ller, Henriette Pilegaard, Steen B. Pedersen, John J. Kopchick, Jens Otto L. Jrgensen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0031857
Abstract: Background Erythropoietin receptors have been identified in human skeletal muscle tissue, but downstream signal transduction has not been investigated. We therefore studied in vivo effects of systemic erythropoietin exposure in human skeletal muscle. Methodology/Principal Findings The protocols involved 1) acute effects of a single bolus injection of erythropoietin followed by consecutive muscle biopsies for 1–10 hours, and 2) a separate study with prolonged administration for 16 days with biopsies obtained before and after. The presence of erythropoietin receptors in muscle tissue as well as activation of Epo signalling pathways (STAT5, MAPK, Akt, IKK) were analysed by western blotting. Changes in muscle protein profiles after prolonged erythropoietin treatment were evaluated by 2D gel-electrophoresis and mass spectrometry. The presence of the erythropoietin receptor in skeletal muscle was confirmed, by the M20 but not the C20 antibody. However, no significant changes in phosphorylation of the Epo-R, STAT5, MAPK, Akt, Lyn, IKK, and p70S6K after erythropoietin administration were detected. The level of 8 protein spots were significantly altered after 16 days of rHuEpo treatment; one isoform of myosin light chain 3 and one of desmin/actin were decreased, while three isoforms of creatine kinase and two of glyceraldehyd-3-phosphate dehydrogenase were increased. Conclusions/Significance Acute exposure to recombinant human erythropoietin is not associated by detectable activation of the Epo-R or downstream signalling targets in human skeletal muscle in the resting situation, whereas more prolonged exposure induces significant changes in the skeletal muscle proteome. The absence of functional Epo receptor activity in human skeletal muscle indicates that the long-term effects are indirect and probably related to an increased oxidative capacity in this tissue.
Genetic Variant SCL2A2 Is Associated with Risk of Cardiovascular Disease – Assessing the Individual and Cumulative Effect of 46 Type 2 Diabetes Related Genetic Variants
Anders Borglykke, Niels Grarup, Thomas Spars?, Allan Linneberg, Mogens Fenger, J?rgen Jeppesen, Torben Hansen, Oluf Pedersen, Torben Jrgensen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0050418
Abstract: Aim To assess the individual and combined effect of 46 type 2 diabetes related risk alleles on incidence of a composite CVD endpoint. Methods Data from the first Danish MONICA study (N = 3523) and the Inter99 study (N = 6049) was used. Using Cox proportional hazard regression the individual effect of each risk allele on incident CVD was analyzed. Risk was presented as hazard ratios (HR) per risk allele. Results During 80,859 person years 1441 incident cases of CVD (fatal and non-fatal) occurred in the MONICA study. In Inter99 942 incident cases were observed during 61,239 person years. In the Danish MONICA study four gene variants were significantly associated with incident CVD independently of known diabetes status at baseline; SLC2A2 rs11920090 (HR 1.147, 95% CI 1.027–1.283 , P = 0.0154), C2CD4A rs7172432 (1.112, 1.027–1.205 , P = 0.0089), GCKR rs780094 (1.094, 1.007–1.188 , P = 0.0335) and C2CD4B rs11071657 (1.092, 1.007–1.183 , P = 0.0323). The genetic score was significantly associated with increased risk of CVD (1.025, 1.010–1.041, P = 0.0016). In Inter99 two gene variants were associated with risk of CVD independently of diabetes; SLC2A2 (HR 1.180, 95% CI 1.038–1.341 P = 0.0116) and FTO (0.909, 0.827–0.998, P = 0.0463). Analysing the two populations together we found SLC2A2 rs11920090 (HR 1.164, 95% CI 1.070–1.267, P = 0.0004) meeting the Bonferroni corrected threshold for significance. GCKR rs780094 (1.076, 1.010–1.146, P = 0.0229), C2CD4B rs11071657 (1.067, 1.003–1.135, P = 0.0385) and NOTCH2 rs10923931 (1.104 (1.001 ; 1.217 , P = 0.0481) were found associated with CVD without meeting the corrected threshold. The genetic score was significantly associated with increased risk of CVD (1.018, 1.006–1.031, P = 0.0043). Conclusions This study showed that out of the 46 genetic variants examined only the minor risk allele of SLC2A2 rs11920090 was significantly (P = 0.0005) associated with a composite endpoint of incident CVD below the threshold for statistical significance corrected for multiple testing. This potential pathway needs further exploration.
Insulin and GH Signaling in Human Skeletal Muscle In Vivo following Exogenous GH Exposure: Impact of an Oral Glucose Load
Thomas Krusenstjerna-Hafstr?m,Michael Madsen,Mikkel H. Vendelbo,Steen B. Pedersen,Jens S. Christiansen,Niels M?ller,Niels Jessen,Jens O. L. Jrgensen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0019392
Abstract: GH induces acute insulin resistance in skeletal muscle in vivo, which in rodent models has been attributed to crosstalk between GH and insulin signaling pathways. Our objective was to characterize time course changes in signaling pathways for GH and insulin in human skeletal muscle in vivo following GH exposure in the presence and absence of an oral glucose load.
Fasting Increases Human Skeletal Muscle Net Phenylalanine Release and This Is Associated with Decreased mTOR Signaling
Mikkel Holm Vendelbo, Andreas Buch M?ller, Britt Christensen, Birgitte Nellemann, Berthil Frederik Forrest Clasen, K. Sreekumaran Nair, Jens Otto Lunde Jrgensen, Niels Jessen, Niels M?ller
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0102031
Abstract: Aim Fasting is characterised by profound changes in energy metabolism including progressive loss of body proteins. The underlying mechanisms are however unknown and we therefore determined the effects of a 72-hour-fast on human skeletal muscle protein metabolism and activation of mammalian target of rapamycin (mTOR), a key regulator of cell growth. Methods Eight healthy male volunteers were studied twice: in the postabsorptive state and following 72 hours of fasting. Regional muscle amino acid kinetics was measured in the forearm using amino acid tracers. Signaling to protein synthesis and breakdown were assessed in skeletal muscle biopsies obtained during non-insulin and insulin stimulated conditions on both examination days. Results Fasting significantly increased forearm net phenylalanine release and tended to decrease phenylalanine rate of disappearance. mTOR phosphorylation was decreased by ~50% following fasting, together with reduced downstream phosphorylation of 4EBP1, ULK1 and rpS6. In addition, the insulin stimulated increase in mTOR and rpS6 phosphorylation was significantly reduced after fasting indicating insulin resistance in this part of the signaling pathway. Autophagy initiation is in part regulated by mTOR through ULK1 and fasting increased expression of the autophagic marker LC3B-II by ~30%. p62 is degraded during autophagy but was increased by ~10% during fasting making interpretation of autophagic flux problematic. MAFbx and MURF1 ubiquitin ligases remained unaltered after fasting indicating no change in protesomal protein degradation. Conclusions Our results show that during fasting increased net phenylalanine release in skeletal muscle is associated to reduced mTOR activation and concomitant decreased downstream signaling to cell growth.
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