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Search Results: 1 - 10 of 9008 matches for " Nicolas Lewis "
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The Construction and Realisation of Geographic Brand Rent in New Zealand Wine
David Hayward,Nicolas Lewis
Urbani Izziv , 2012,
Abstract: This paper examines the concept of geographic brand rent in production and marketing of New Zealand wine. It is argued that the region of origin for fine wine is not only a recognised feature of its value but that this may be conceived as a form of brand rent and furthermore that it is measurable. The analysis uses both cross-sectional and longitudinal approaches to consider grape and vineyard land prices, as well as interviews with industry informants to explore wine company strategies. Explicit use is made of the value-added chain in order to identify and situate the construction of rents and the positionality of industry actors in respect to these. The analysis highlights the quest to control different forms of brand rent that have led the restructuring of the wine industry. It also exposes countervailing pressures towards economic resilience and market vulnerability facing the fine wine industry.
Feeling the pull, a study of natural Galactic accelerometers. I: photometry of the delicate stellar stream of the Palomar 5 globular cluster
Rodrigo A. Ibata,Geraint F. Lewis,Nicolas F. Martin
Physics , 2015,
Abstract: We present an analysis of wide-field photometric surveys of the Palomar 5 globular cluster and its stellar stream, based on g- and r-band measures together with narrow-band DDO51 photometry. In this first study, we use the deep (g,r) data to measure the incidence of gaps and peaks along the stream. Examining the star-counts profile of the stream plus contaminating populations, we find no evidence for significant under-densities, and find only a single significant over-density. This is at odds with earlier studies based on matched-filter maps derived from shallower SDSS data if the contaminating population possesses plausible spatial properties. The lack of substantial sub-structure along the stream may be used in future dynamical simulations to examine the incidence of dark matter sub-halos in the Galactic halo. We also present a measurement of the relative distances along the stream which we use to create the deepest wide-field map of this system to date.
Microfabricated Modular Scale-Down Device for Regenerative Medicine Process Development
Marcel Reichen, Rhys J. Macown, Nicolas Jaccard, Alexandre Super, Ludmila Ruban, Lewis D. Griffin, Farlan S. Veraitch, Nicolas Szita
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0052246
Abstract: The capacity of milli and micro litre bioreactors to accelerate process development has been successfully demonstrated in traditional biotechnology. However, for regenerative medicine present smaller scale culture methods cannot cope with the wide range of processing variables that need to be evaluated. Existing microfabricated culture devices, which could test different culture variables with a minimum amount of resources (e.g. expensive culture medium), are typically not designed with process development in mind. We present a novel, autoclavable, and microfabricated scale-down device designed for regenerative medicine process development. The microfabricated device contains a re-sealable culture chamber that facilitates use of standard culture protocols, creating a link with traditional small-scale culture devices for validation and scale-up studies. Further, the modular design can easily accommodate investigation of different culture substrate/extra-cellular matrix combinations. Inactivated mouse embryonic fibroblasts (iMEF) and human embryonic stem cell (hESC) colonies were successfully seeded on gelatine-coated tissue culture polystyrene (TC-PS) using standard static seeding protocols. The microfluidic chip included in the device offers precise and accurate control over the culture medium flow rate and resulting shear stresses in the device. Cells were cultured for two days with media perfused at 300 μl.h?1 resulting in a modelled shear stress of 1.1×10?4 Pa. Following perfusion, hESC colonies stained positively for different pluripotency markers and retained an undifferentiated morphology. An image processing algorithm was developed which permits quantification of co-cultured colony-forming cells from phase contrast microscope images. hESC colony sizes were quantified against the background of the feeder cells (iMEF) in less than 45 seconds for high-resolution images, which will permit real-time monitoring of culture progress in future experiments. The presented device is a first step to harness the advantages of microfluidics for regenerative medicine process development.
Celsr3 Is Required for Normal Development of GABA Circuits in the Inner Retina
Alaron Lewis,Neil Wilson,George Stearns,Nicolas Johnson,Ralph Nelson,Susan E. Brockerhoff
PLOS Genetics , 2011, DOI: 10.1371/journal.pgen.1002239
Abstract: The identity of the specific molecules required for the process of retinal circuitry formation is largely unknown. Here we report a newly identified zebrafish mutant in which the absence of the atypical cadherin, Celsr3, leads to a specific defect in the development of GABAergic signaling in the inner retina. This mutant lacks an optokinetic response (OKR), the ability to visually track rotating illuminated stripes, and develops a super-normal b-wave in the electroretinogram (ERG). We find that celsr3 mRNA is abundant in the amacrine and ganglion cells of the retina, however its loss does not affect synaptic lamination within the inner plexiform layer (IPL) or amacrine cell number. We localize the ERG defect pharmacologically to a late-stage disruption in GABAergic modulation of ON-bipolar cell pathway and find that the DNQX-sensitive fast b1 component of the ERG is specifically affected in this mutant. Consistently, we find an increase in GABA receptors on mutant ON-bipolar terminals, providing a direct link between the observed physiological changes and alterations in GABA signaling components. Finally, using blastula transplantation, we show that the lack of an OKR is due, at least partially, to Celsr3-mediated defects within the brain. These findings support the previously postulated inner retina origin for the b1 component and reveal a new role for Celsr3 in the normal development of ON visual pathway circuitry in the inner retina.
Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesis
Raymond C Pasek, Nicolas F Berbari, Wesley R Lewis, Robert A Kesterson, Bradley K Yoder
Cilia , 2012, DOI: 10.1186/2046-2530-1-20
Abstract: Cluap1 mutant embryos were generated and examined for gross morphological and anatomical defects using light microscopy. Reverse transcription PCR, β-galactosidase staining assays, and immunofluorescence analysis were used to determine the expression of the gene and localization of the protein in vivo and in cultured cell lines. We also used immunofluorescence analysis and qRT-PCR to examine defects in the Sonic hedgehog signaling pathway in mutant embryos.Cluap1 mutant embryos die in mid-gestation, indicating that it is necessary for proper development. Mutant phenotypes include a failure of embryonic turning, an enlarged pericardial sac, and defects in neural tube development. Consistent with the diverse phenotypes, Cluap1 is widely expressed. Furthermore, the Cluap1 protein localizes to primary cilia, and mutant embryos were found to lack cilia at embryonic day 9.5. The phenotypes observed in Cluap1 mutant mice are indicative of defects in Sonic hedgehog signaling. This was confirmed by analyzing hedgehog signaling activity in Cluap1 mutants, which revealed that the pathway is repressed.These data indicate that the function of Cluap1 is evolutionarily conserved with regard to ciliogenesis. Further, the results implicate mammalian Cluap1 as a key regulator of hedgehog signaling and as an intraflagellar transport B complex protein. Future studies on mammalian Cluap1 utilizing this mouse model may provide insights into the role for Cluap1 in intraflagellar transport and the association with colon cancer and cystic kidney disorders.Cilia are complex organelles requiring hundreds of different genes for their assembly and function [1]. The assembly of the cilium is dependent on intraflagellar transport (IFT), a molecular motor-driven process that mediates the bidirectional movement of proteins between the base and tip of the cilium [2,3]. IFT was initially described in the green algae Chlamydomonas reinhardtii and subsequently in multiple other ciliated eukaryotes, the
Does the Sagittarius Stream constrain the Milky Way halo to be triaxial?
Rodrigo Ibata,Geraint F. Lewis,Nicolas F. Martin,Michele Bellazzini,Matteo Correnti
Physics , 2012, DOI: 10.1088/2041-8205/765/1/L15
Abstract: Recent analyses of the stellar stream of the Sagittarius dwarf galaxy have claimed that the kinematics and three-dimensional location of the M-giant stars in this structure constrain the dark matter halo of our Galaxy to possess a triaxial shape that is extremely flattened, being essentially an oblate ellipsoid oriented perpendicular to the Galactic disk. Using a new stream-fitting algorithm, based on a Markov Chain Monte Carlo procedure, we investigate whether this claim remains valid if we allow the density profile of the Milky Way halo greater freedom. We find stream solutions that fit the leading and trailing arms of this structure even in a spherical halo, although this would need a rising Galactic rotation curve at large Galactocentric radius. However, the required rotation curve is not ruled out by current constraints. It appears therefore that for the Milky Way, halo triaxiality, despite its strong theoretical motivation, is not required to explain the Sagittarius stream. This degeneracy between triaxiality and the halo density profile suggests that in future endeavors to model this structure, it will be advantageous to relax the strict analytic density profiles that have been used to date.
Eppur si muove: Positional and kinematic correlations of satellite pairs in the low Z universe
Rodrigo A. Ibata,Benoit Famaey,Geraint F. Lewis,Neil G. Ibata,Nicolas Martin
Physics , 2014, DOI: 10.1088/0004-637X/805/1/67
Abstract: We have recently shown (Ibata et al. 2014) that pairs of satellite galaxies located diametrically opposite each other around their host possess predominantly anti-correlated velocities. This is consistent with a scenario in which $\sim 50$% of satellite galaxies belong to kinematically-coherent rotating planar structures, similar to those detected around the giant galaxies of the Local Group. Here we extend this analysis, examining the incidence of satellites of giant galaxies drawn from an SDSS photometric redshift catalog. We find that there is a $\sim 17$% overabundance ($> 3 \sigma$ significance) of candidate satellites at positions diametrically opposite a spectroscopically confirmed satellite. We show that cosmological simulations do not possess this property when the contamination is included, and that there are in fact, after subtracting contamination, 2 to 3 times more satellites diametrically opposed to a spectroscopically confirmed satellite than at $90\deg$ from it. We also examine the correlation between the satellite pair positions and the orientation of the host galaxy major axis. We find that those satellite pairs with anti-correlated velocities have a strong preference ($\sim 3:1$) to align with the major axis of the host whereas those with correlated velocities display the opposite behavior. This correlation of the satellite alignments appears to be stronger than the well-documented preference of satellites to be located close to the major axis of their host. We finally show that repeating a similar analysis to Ibata et al. (2014) with same-side satellites is generally hard to interpret, but is not inconsistent with our previous results when strong quality-cuts are applied on the sample. All these unexpected correlations strongly suggest that a substantial fraction of satellite galaxies are causally-linked in their formation and evolution.
N. elongata Produces Type IV Pili That Mediate Interspecies Gene Transfer with N. gonorrhoeae
Dustin L. Higashi, Nicolas Biais, Nathan J. Weyand, Al Agellon, Jennifer L. Sisko, Lewis M. Brown, Magdalene So
PLOS ONE , 2011, DOI: 10.1371/journal.pone.0021373
Abstract: The genus Neisseria contains at least eight commensal and two pathogenic species. According to the Neisseria phylogenetic tree, commensals are basal to the pathogens. N. elongata, which is at the opposite end of the tree from N. gonorrhoeae, has been observed to be fimbriated, and these fimbriae are correlated with genetic competence in this organism. We tested the hypothesis that the fimbriae of N. elongata are Type IV pili (Tfp), and that Tfp functions in genetic competence. We provide evidence that the N. elongata fimbriae are indeed Tfp. Tfp, as well as the DNA Uptake Sequence (DUS), greatly enhance N. elongata DNA transformation. Tfp allows N. elongata to make intimate contact with N. gonorrhoeae and to mediate the transfer of antibiotic resistance markers between these two species. We conclude that Tfp functional for genetic competence is a trait of a commensal member of the Neisseria genus. Our findings provide a mechanism for the horizontal gene transfer that has been observed among Neisseria species.
A New Collisional Ring Galaxy at z = 0.111: Auriga's Wheel
Blair C. Conn,Anna Pasquali,Emanuela Pompei,Richard R. Lane,André-Nicolas Chené,Rory Smith,Geraint F. Lewis
Physics , 2011, DOI: 10.1088/0004-637X/741/2/80
Abstract: We report the serendipitous discovery of a collision ring galaxy, identified as 2MASX J06470249+4554022, which we have dubbed 'Auriga's Wheel', found in a SUPRIME-CAM frame as part of a larger Milky Way survey. This peculiar class of galaxies is the result of a near head-on collision between typically, a late type and an early type galaxy. Subsequent GMOS-N long-slit spectroscopy has confirmed both the relative proximity of the components of this interacting pair and shown it to have a redshift of 0.111. Analysis of the spectroscopy reveals that the late type galaxy is a LINER class Active Galactic Nuclei while the early type galaxy is also potentially an AGN candidate, this is very uncommon amongst known collision ring galaxies. Preliminary modeling of the ring finds an expansion velocity of ~200 kms^-1 consistent with our observations, making the collision about 50 Myr old. The ring currently has a radius of about 10 kpc and a bridge of stars and gas is also visible connecting the two galaxies.
A thousand shadows of Andromeda: rotating planes of satellites in the Millennium-II cosmological simulation
Rodrigo A. Ibata,Neil G. Ibata,Geraint F. Lewis,Nicolas F. Martin,Anthony Conn,Pascal Elahi,Veronica Arias,Nuwanthika Fernando
Physics , 2014, DOI: 10.1088/2041-8205/784/1/L6
Abstract: In a recent contribution, Bahl \& Baumgardt investigated the incidence of planar alignments of satellite galaxies in the Millennium-II simulation, and concluded that vast thin planes of dwarf galaxies, similar to that observed in the Andromeda galaxy (M31), occur frequently by chance in $\Lambda$-Cold Dark Matter cosmology. However, their analysis did not capture the essential fact that the observed alignment is simultaneously radially extended, yet thin, and kinematically unusual. With the caveat that the Millennium-II simulation may not have sufficient mass resolution to identify confidently simulacra of low-luminosity dwarf galaxies, we re-examine that simulation for planar structures, using the same method as employed by Ibata et al. (2013) on the real M31 satellites. We find that 0.04\% of host galaxies display satellite alignments that are at least as extreme as the observations, when we consider their extent, thickness and number of members rotating in the same sense. We further investigate the angular momentum properties of the co-planar satellites, and find that the median of the specific angular momentum derived from the line of sight velocities in the real M31 structure ($1.3\times10^4$ km/s kpc) is very high compared to systems drawn from the simulations. This analysis confirms that it is highly unlikely that the observed structure around the Andromeda galaxy is due to a chance occurrence. Interestingly, the few extreme systems that are similar to M31 arise from the accretion of a massive sub-halo with its own spatially-concentrated entourage of orphan satellites.
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