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Search Results: 1 - 10 of 26638 matches for " Nascimento Vladimir Pinheiro do "
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Salmonella spp. in raw broiler parts: occurrence, antimicrobial resistance profile and phage typing of the Salmonella Enteritidis isolates
Ribeiro, Aldemir Reginato;Kellermann, Aline;Santos, Luciana Ruschel dos;Bessa, Marjo Cadó;Nascimento, Vladimir Pinheiro do;
Brazilian Journal of Microbiology , 2007, DOI: 10.1590/S1517-83822007000200021
Abstract: the present study was carried out to evaluate the occurrence of salmonellae in raw broiler parts and to determine the antimicrobial resistance profile of the isolated strains. twenty-four (39.3%) broiler parts samples were positive for salmonella and twenty-five salmonella strains were isolated, since two different serovars were detected in one single positive sample. salmonella enteritidis was the most prevalent serovar. among salmonella enteritidis isolates, 95.2% belonged to phage type 4 (pt4) (20/21) and 4.8% to pt7 (1/21). twenty-two (88%) strains of salmonella were resistant to at least one antimicrobial agent, generating eight different resistance patterns. the s. typhimurium (n: 1) and s. hadar (n: 3) isolates presented multiple resistance. three s. enteritidis isolates were susceptible to all antimicrobials tested, two were resistant only to tetracycline. the high prevalence of salmonella in the broiler parts strenghtens the importance of the use of good manufacturing practices (gmp), and haccp. the results also emphasize the need for the responsible use of antimicrobials in animal production.
Número mais provável de Salmonella isoladas de carca?as de frango resfriadas
Borsoi, Anderlise;Moraes, Hamilton Luis de Souza;Salle, Carlos Tadeu Pippi;Nascimento, Vladimir Pinheiro do;
Ciência Rural , 2010, DOI: 10.1590/S0103-84782010001100014
Abstract: salmonella in poultry remains an important worldwide problem, and among foodborne pathogens, the salmonella appears as one of the most important outbreaks agents. to assess the risks of acquiring infection via undercooked poultry or cross contamination from chickens, it is important to determine the extent of the contamination on raw poultry with this pathogen. in this study, 180 refrigerated broiler carcasses, obtained from local stores, were assessed to recover salmonella by the most probable number (mpn) method to quantify bacterias cells onto brilliant green agar with novobiocin (bgn) and xylose lysin tergitol 4 agar (xlt4). the results showed 12,2% occurrence of salmonella by conventional microbiological method from refrigerated broiler carcasses. the mpn per ml rates was 2,7 cells on xlt4 agar and 1,3 cells on bgn agar plate. the salmonella serovars isolated from broiler carcasses were s. enteritidis, s. agona, s. rissen, s. heidelberg and s. livingstone. results analysis showed that could be a variable number of cells contaminating refrigerated broiler carcasses, which have been selling to the consumer.
Caracteriza??o do consumidor de carne de frango da cidade de Porto Alegre
Francisco, Dione Carina;Nascimento, Vladimir Pinheiro do;Loguercio, Andrea Pinto;Camargo, Luciane;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000100041
Abstract: the concern with the alimentary security has changed the form as the consumers see the meaty products; searching information on the foods that consume. in this direction, this research was aimed at characterizing the chicken meat consumer of porto alegre, brazil. they had been interviewed 393 consumers during the period of april the july of 2004. the results demonstrate that the chicken meat is the second preferred meat of the consumers, and that the empanados cuts and of chicken are the most consumed products. the consumers believe that the bird flu and salmonelose are the main illnesses propagated by this meat.
Polymerase chain reaction (PCR) for the detection of Salmonella in artificially inoculated chicken meat
SANTOS Luciana Ruschel dos,NASCIMENTO Vladimir Pinheiro do,OLIVEIRA Sílvia Dias de,FLORES Maristela Lovato
Revista do Instituto de Medicina Tropical de S?o Paulo , 2001,
Abstract: The aim of this study was to develop a polymerase chain reaction (PCR) protocol for the detection of Salmonella in artificially contaminated chicken meat. Tests were performed with different dilutions of Salmonella Typhimurium or Salmonella Enteritidis cells (10-7, 10-8 or 10-9 CFU/mL) inoculated in chicken meat samples, in order to establish the limits of detection, incubation times (0, 6, 8 and 24 hours of pre-enrichment in PBW 1%) and three DNA extraction protocols (phenol-chloroform, thermal treatment and thermal treatment and Sephaglass). The assay was able to detect until 10-9 CFU/mL of initial dilution of Salmonella cells inoculated in chicken meat, which allows detection of Salmonella within 48 hours, including 24 hours of pre-enrichment and using the phenol-chloroform DNA extraction protocol. As the results are obtained in a shorter time period than that of microbiological culture, this procedure will be useful in the methodology for detection of Salmonella in chicken.
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
Ribeiro Aldemir Reginato,Nascimento Vladimir Pinheiro do,Cardoso Martha Oliveira,Santos Luciana Ruschel dos
Brazilian Journal of Microbiology , 2002,
Abstract: This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.
Macroscopia, histopatologia e bacteriologia de fígados de frangos (Gallus gallus) condenados no abate
Barcelos Aleverson da Silva,Fl?res Maristela Lovato,Kommers Glaucia Denise,Nascimento Vladimir Pinheiro do
Ciência Rural , 2006,
Abstract: Este trabalho avaliou, através da macroscopia, histopatologia e bacteriologia, fígados de frangos (Gallus gallus) condenados no abate. Cem fígados de frango foram coletados na linha de inspe o. Noventa deles tinham sido condenados por apresentarem altera es macroscópicas. Dez fígados n o apresentavam altera es macroscópicas. As coletas foram feitas em dois abatedouros localizados no Estado do Rio Grande do Sul. No laboratório, fez-se detalhada descri o das altera es macroscópicas dos fígados condenados e exames histopatológicos e bacteriológicos complementares. Para a descri o macroscópica, os seguintes parametros foram avaliados: forma, colora o, tamanho, consistência, odor e presen a de les es visíveis. Foram observados fígados com altera es na cor, forma, tamanho e/ou consistência em 47/90 amostras; fígados marrom-pálidos e com outras altera es macroscópicas associadas perfizeram 19/90 amostras; 5/90 fígados estavam amarelos ou amarelados e com outras altera es macroscópicas associadas; 19/90 fígados estavam verdes ou esverdeados e com outras altera es macroscópicas associadas. Os principais diagnósticos histopatológicos foram de colangio-hepatite heterofílica multifocal, degenera o e/ou necrose hepatocelular centrolobular e em ponte, hepatite necrosante aleatória, pericolangite heterofílica multifocal e peri-hepatite fibrinosa subaguda difusa acentuada. A bacteriologia foi direcionada para pesquisa de Escherichia coli, Salmonella spp. e Staphylococcus sp. Utilizando-se cultivo direto das amostras de fígado em meios de cultura seletivos, isolou-se E. coli em 26/100 amostras e Staphylococcus sp. em 24/100 amostras. Para pesquisa de salmonelas, utilizou-se a metodologia convencional preconizada para esta bactéria, entretanto n o houve isolamento de nenhuma espécie deste gênero. Através das avalia es realizadas pode-se confirmar o predomínio de les es sugestivas de infec o bacteriana nos fígados condenados.
Análise da contamina o por Salmonella em ovos do tipo colonial através da rea o em cadeia da polimerase
Fl?res Maristela Lovato,Nascimento Vladimir Pinheiro do,Kader Ivonyr Irene Tróglio Abdel,Cardoso Martha
Ciência Rural , 2003,
Abstract: A identifica o de poedeiras comerciais infectadas por salmonelas tem sido um dos pontos fortes da profilaxia e conseqüente redu o de surtos de salmonelose em humanos associados ao consumo de ovos, sendo que a análise dos ovos pode ser mais um dos pontos de detec o da infec o, que, muitas vezes, cursa sem sinais clínicos. A Rea o em Cadeia da Polimerase (PCR) parece ser uma estratégia útil para detec o de Salmonella, pois vários autores têm utilizado a PCR para verificar a presen a da bactéria em carnes, fezes, tecidos, sangue, leite e ovos, com diferentes metodologias de manipula o das amostras. Foram analisados 360 ovos, procedentes de dez propriedades rurais, produtoras de ovos tipo colonial, no distrito de Camobi, em Santa Maria - RS. Os ovos foram divididos em grupos de seis, totalizando sessenta amostras. O exame bacteriológico foi realizado conforme metodologia preconizada pelas normas técnicas e a metodologia de extra o de DNA pelo fenol-clorofórmio. A PCR foi realizada para a amplifica o de um fragmento de DNA de 284 pb. A análise dos resultados n o demonstrou diferen a significativa entre a PCR e o bacteriológico. Todas as amostras positivas ao bacteriológico foram positivas na PCR, sendo que essa última detectou duas amostras a mais, devido a sua alta sensibilidade e especificidade, especialmente quando é sabido que os ovos apresentam uma popula o microbiana mista que, muitas vezes, impede o isolamento adequado das salmonelas no bacteriológico pela competi o com a flora bacteriana normalmente presente.
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
Ribeiro, Aldemir Reginato;Nascimento, Vladimir Pinheiro do;Cardoso, Martha Oliveira;Santos, Luciana Ruschel dos;Rocha, Silvio Luís da Silveira;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000400012
Abstract: this study was conducted aiming to compare the conventional microbiological method to detect salmonella in broiler parts with the immunomagnetic separation method (ims) followed by plate isolation and also the ims associated with rappaport-vassiliadis broth (rv). the ims was performed following a pre- enrichment step in buffered peptone water. sixty-one samples (raw broiler parts) were tested and the results showed that the use of the ims method alone allowed the isolation of salmonella in 9 of the tested samples, while the association ims/rv detected the agent in 30 samples. the conventional microbiological method was able to isolate the agent in 25 opportunities. these results allowed to conclude that the ims/rv association presented an increased sensitivity and permitted a better isolation of salmonella. the conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded salmonella, should be searched.
Avalia??o in vitro de clorexidina, am?nia quaternária e ácido peracético frente a amostras de Salmonella Heidelberg isoladas de abatedouro avícola em 2005 e 2009
Colla, Fernanda Lúcia;Rodrigues, Laura B.;Dickel, Elci Lotar;Borsoi, Anderlise;Nascimento, Vladimir Pinheiro do;Santos, Luciana Ruschel dos;
Pesquisa Veterinária Brasileira , 2012, DOI: 10.1590/S0100-736X2012000400003
Abstract: the objective of this investigation was to evaluate the in vitro sensibility of salmonella heidelberg to three commercially available disinfectants used for sanitization in poultry slaughterhouses. a total of 20 s. heidelberg were tested (14 isolated in 2005 and six in 2009), and as active ingredients were used chlorhexidine (0.5%), quaternary ammonium (0.5 %) and peracetic acid (1%) at contact intervals of 5, 10, 15 e 20 minutes. all isolates were found to be sensitive to peracetic acid at four specific contact intervals. one hundred percent of s. heidelberg isolated in 2005 was found to be sensitive to quaternary ammonium, while 33% of 2009 isolates were resistant at a 5-minute contact interval and 16.6% at 10-minutes. with respect to chlorhexidine, 25% of the 2005 isolates were resistant at a 5-minute contact interval, 33% of the 2009 isolates were resistant with the same time, and 17% at a 10-minute contact interval. it can be concluded that the highest disinfectant activity in vitro was found to be with peracetic acid for s. heidelberg isolates in 2005 and 2009, whereas chlorhexidine and quaternary ammonium had a reduced action against 2009 isolates, indicating the progression of bacterial resistance against these sanitizers and the need for periodic evaluation and rotation of active principles for sanitization.
Phage types of Salmonella Enteritidis isolated from clinical and food samples, and from broiler carcasses in Southern Brazil
Santos Luciana Ruschel dos,Nascimento Vladimir Pinheiro do,Oliveira Sílvia Dias de,Rodrigues Dália P.
Revista do Instituto de Medicina Tropical de S?o Paulo , 2003,
Abstract: 272 isolates of Salmonella Enteritidis (111 isolated from frozen broiler chicken carcasses, 126 from human food and other biological materials involved in food poisoning outbreaks and 35 from different poultry materials) were selected for phage typing. From these, 111 were phage typed, 57.65% being classified as phage type 4, 32.43% as phage type 4a, 3.60% as phage type 6a and 0.90% as phage type 7, whereas 5.40% samples were not phage typeable. The predominance of phage type 4 is in agreement with the results published worldwide, and reinforces the need for studies related to the epidemiological meaning of these findings.
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