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Search Results: 1 - 10 of 565709 matches for " Nascimento Maria da Gra?a Fichel do "
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Detection of Mycoplasma pulmonis in laboratory rats
Barreto Maria Lucia,Nascimento Elmiro Rosendo do,Campos Carlos Augusto de Martino,Nascimento Maria da Graa Fichel do
Brazilian Journal of Microbiology , 2002,
Abstract: This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. The ELISA test was carried out in 20 animals of both colonies. In the conventional controlled premises, 92.86% (13/14) were positive for M. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. The results confirmed that M. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the ELISA positivity rated higher than culture.
Mycoplasma synoviae infection on Newcastle disease vaccination of chickens
Silva, Rita de Cássia Figueira;Nascimento, Elmiro Rosendo do;Pereira, Virgínia Léo de Almeida;Barreto, Maria Lúcia;Nascimento, Maria da Graa Fichel do;
Brazilian Journal of Microbiology , 2008, DOI: 10.1590/S1517-83822008000200033
Abstract: newcastle disease is characterized by respiratory manifestations in association with nervous and/or digestive symptoms. its prevention is done by vaccination with live attenuated (lentogenic strains) and/or killed vaccines. the lentogenic strains can lead to strong post-vaccination reaction, principally due to the presence of other pathogenic agents. among them, mycoplasma synoviae is worldwide important, mainly in brazil. the dissemination of this agent in poultry flocks has been achieved due to difficulties in diagnosis and disease reproduction, virulence variations among different m.synoviae strains, and attribution of typical m.synoviae disease manifestation to other disease agents. this experimental study in spf chicks (gallus gallus), previously infected by m.synoviae and thereafter vaccinated against newcastle disease, was done with the objective of evaluating m.synoviae pathogenicity through assessment of post-vaccinal respiratory reactions and serologic responses to newcastle disease virus vaccine in the absence of environmental factors. a total of 86 three days old chicks were used, being 57 infected by eye and nostril drop, with chicken activated m. synoviae strain wvu 1853. seven days later, 21 mycoplasma infected birds plus 29 not mycoplasma infected ones were vaccinated against newcastle disease. as results, the not infected and vaccinated birds yielded, significantly, higher and longer lasting serologic responses to newcastle disease vaccine virus than those infected and vaccinated. similarly, the infected and vaccinated birds yielded lower serologic reactions to m.synoviae than those only mycoplasma infected. no post-vaccinal respiratory reaction was observed in the vaccinated birds.
Detection of Mycoplasma pulmonis in laboratory rats
Barreto, Maria Lucia;Nascimento, Elmiro Rosendo do;Campos, Carlos Augusto de Martino;Nascimento, Maria da Graa Fichel do;Lignon, Gilberto Brasil;Lira, Marie Luce Flores;Silva, Ricardo G.;
Brazilian Journal of Microbiology , 2002, DOI: 10.1590/S1517-83822002000300015
Abstract: this work was conducted on rats in two premises located in niterói and rio de janeiro, brazil. one is classified as conventional controlled and the other, conventional. the objective of the present study was to detect the presence of mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. in the conventional controlled premises, 16 rats of wistar-furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of hooded lister strain. the clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. from 28 rats, 57.14% (16/28) were culture positive for m. pulmonis, being 81.25% (13/16) from the conventional controlled premises, and 25.00% (3/12) from the conventional premises. the elisa test was carried out in 20 animals of both colonies. in the conventional controlled premises, 92.86% (13/14) were positive for m. pulmonis, and 7.14% (1/14) were suspicious, while in the conventional premises, 100% (6/6) of the samples were positive. the results confirmed that m. pulmonis was the etiologic agent of the disease that affected the rats under study, and that the elisa positivity rated higher than culture.
Anofelinos de Santa Catarina (Diptera: Culicidae), Brasil
Portes, Maria da Graa Teixeira;Rossi, Juliana Chedid Nogared;Nascimento, Jo?o Cezar do;Zeccer, Suzana;Silva, Luis Antonio;
Revista da Sociedade Brasileira de Medicina Tropical , 2010, DOI: 10.1590/S0037-86822010000200010
Abstract: introduction: the amazon region of brazil is endemic for malaria. in the state of santa catarina, malaria was eliminated in the 1980s. since then, a few sporadic isolated autochthonous cases have occurred. however, because malaria vectors are present within brazilian territory and extensive endemic areas exist in this country, along with the great mobility of people in tourist areas of santa catarina, there is the likelihood of reintroduction of the disease. methods: the following data were used: the database of the entomology group of the national health foundation, santa catarina (acces, 1997-2000); the epidemiological surveillance information system of the health surveillance department (malaria/sc); and the notifiable disease information system (sinan/sc). these data were transferred to and analyzed in the microsoft office excel 2007 software. results: collections were carried out in 48 municipalities and 159 localities, and 12,310 culicids, 11,546 anophelines (93.7%) and 764 others (6.2%) were identified. three subgenera and 13 species of anophelines were identified. conclusions: given that in the municipalities investigated, important vectors such as anopheles cruzii and anopheles albitasis were found to be present, with movements of infected individuals from endemic areas, these areas can be considered to be receptive and vulnerable to malaria. these species are suspected of being responsible for malaria transmission in this region, especially in the municipalities of gaspar, indaial and rodeio.
Situa??o epidemiológica da leishmaniose visceral, na Ilha de S?o Luís, Estado do Maranh?o
Silva, Antonio Rafael da;Tauil, Pedro Luiz;Cavalcante, Maria Neuza Souza;Medeiros, Maria Nilza;Pires, Benedito Nascimento;Gon?alves, Eloísa da Graa do Rosário;
Revista da Sociedade Brasileira de Medicina Tropical , 2008, DOI: 10.1590/S0037-86822008000400007
Abstract: the results from a field study on autochthonous visceral leishmaniasis on the island of s?o luís are presented. this study started in 2004 and finished in 2006 and had the aim of ascertaining the determinant epidemiological and clinical characteristics of this endemic disease. two hundred ninety nine autochthonous cases were analyzed, of which 83.6% were children younger than 9 years old and 54.1% were male. the disease occurred in all months of the year, with a peak in june. the coefficient of incidence decreased from 46.1 to 35.2 cases per 100,000 inhabitants over the years studied. the diagnosis was confirmed by laboratory tests in 93.3% of the cases. the treatment of choice was based on n-methylglucamine, with a cure rate of 96.1%. the mean lethality rate was 3.7%. because of the absence of systematic control actions, the authors propose the creation of a specific program to be developed by the municipalities under the coordination of the state department of health.
Estudo comparativo entre tratamento convencional e tratamento com heparina tópica para a analgesia de queimaduras
Barretto, Marcos Guilherme Praxedes;Costa, Maria da Graa Nascimento Figueira;Serra, Maria Cristina do Valle Freitas;Afiune, Jorge Barros;Praxedes, Hugo Eduardo Pinheiro;Pagani, Eduardo;
Revista da Associa??o Médica Brasileira , 2010, DOI: 10.1590/S0104-42302010000100016
Abstract: objective: this was a prospective, randomized, open-label study controlled by active comparator. the aim was to assess analgesic efficacy and overall tolerability of a burn treatment based on topic administration of unfractionated heparin. methods: fifty eight male or female patients were randomized for conventional treatment (group c) or topical heparin treatment (group th). ages of patients enrolled ranged from 18 to 55 years. they had 2nd and 3rd degree burns on 10% to 30% of the body surface (bs) caused by fire or scald, no hemorrhagic diseases, no hypersensitivity to heparin and less than 10% of the bs burned to 3rd degree. the group c had frequent debridement under anesthesia or analgesia and received silver sulfadiazine dressings. the group th had the first debridement and their wounds left open to receive 4200 iu of unfractionated heparin topically for each 1% of burned bs, three times daily. efficacy was evaluated from files of the 38 patients who completed the study according to demand of analgesic medications and response to the pain visual analog scale (vas). tolerability was evaluated from the files of all 58 randomized patients by the comparative incidence of adverse reactions. results: the group th demanded less analgesic medications (11.83 ± 9.38 per patient against 33.35 ± 20.63 for the c group, p<0.01), reported less pain in the vas, had less fever and more bleeding than group c. there was no difference in the incidence of local infection, septicemia and safety exams. conclusion: the group th presented less pain without important tolerability problems.
Leishmaniose visceral (calazar) na Ilha de S?o Luís, Maranh?o, Brasil: evolu??o e perspectivas
Silva, Antonio Rafael da;Viana, Graa Maria Castro;Varonil, Carlos;Pires, Benedito;Nascimento, Maria do Desterro S.D.;Costa, Jackson M.L.;
Revista da Sociedade Brasileira de Medicina Tropical , 1997, DOI: 10.1590/S0037-86821997000500002
Abstract: visceral leishmaniasis (kala-azar) was definitively an important disease of the state of maranh?o-brasil since 1982. since of then, many authors have been working with this topic in spite of reports. nevertheless, the aspects of diagnosis, treatment an control of cure went through still hard worried the authors have been studing the disease, that came at s?o luís island since of the desestabilization of the ecotops of lutzomya longipalpis, most important sandflies bites. after 1993 the constatation of cases with bad response to pentavalent antimonial (glucantime?) comes to add the other worries. this actual trial accost the disease and conclude about an existence of failures to glucantime? being important to have much vigilance in the diagnosis, treatment and control of cure of the patients.
Estudo comparativo de anticorpos IgG e IgE antileishmania como marcadores de infec??o e doen?a em indivíduos de área endêmica de leishmaniose visceral, em S?o Luis, MA
Nascimento, Maria do Desterro Soares Brand?o;Bezerra, Geusa Felipa de Barros;Bandeira Neto, Abderval Pinto;Silva, Leopoldo Muniz da;Bezerra, José de Macêdo;Viana, Graa Maria de Castro;
Revista da Sociedade Brasileira de Medicina Tropical , 2006, DOI: 10.1590/S0037-86822006000100007
Abstract: comparative study regarding igg and ige anti-leishmania as markers of infection and illness among residents of a visceral leishmaniasis visceral leishmaniasis endemic area, s?o luis, ma in the period from may 1999 to may 2000. all the 1,016 individuals younger than 16 years old were tested for the presence of igg by elisa. a total of 174 (17,1%) children revealed a positive igg test and 4 children showed symptoms of classical visceral leishmaniasis during the time of the survey: 85 ige anti-leishmania elisa tests with positivity of 43,5% were realized. in this sample, all 7 children with past-visceral leishmaniasis in the sample were igg positive and 4 (57,1%) were ige positive, even after a 7 year post treatment period. three children with current visceral leishmaniasis were evaluated, and all of them were positive for both tests. the detection of antileishman ige antibodies presented as a good marker for infection by leishmania chagasi in endemic areas but not as a disease marker.
Prevalência de infec??o por Leishmania chagasi utilizando os métodos de ELISA (rK39 e CRUDE) e intradermorrea??o de Montenegro em área endêmica do Maranh?o, Brasil
Nascimento, Maria do Desterro Soares Brand?o;Souza, Edilberto Costa;Silva, Leopoldo Muniz da;Leal, Plinio da Cunha;Cantanhede, Karleno de Lima;Bezerra, Geusa Felipa de Barros;Viana, Graa Maria de Castro;
Cadernos de Saúde Pública , 2005, DOI: 10.1590/S0102-311X2005000600028
Abstract: a prospective study was undertaken in 1,520 children less than 15 years of age in s?o josé de ribamar, maranh?o, brazil, from june 1994 to january 1995, to evaluate the prevalence and characteristics (socioeconomic, environmental, and behavioral) associated with infection by leishmania chagasi. montenegro skin test (mst) and enzyme-linked immunosorbent assay (elisa-rk39 and crude) test were used to detect infection. the statistical analysis used the c2 test with yates correction and a p value less than 0.05 was considered statistically significant. prevalence of infection was 61.7% as measured by mst, 19.4% according to elisa (rk39), and 19.7% by elisa (crude). association was detected between leishmaniasis in the family, water supply, application of insecticide, and infection by l. chagasi using mst. no association with infection by l. chagasi was detected using elisa rk39 or crude. more effective control measures are needed to reduce prevalence and to detect asymptomatic cases in this high percentage of infected children.
A aprendizagem profissional - as representa??es de treinadores desportivos de jovens: quatro estudos de caso
Ramos, Valmor;Graa, Amandio Braga dos Santos;Nascimento, Juarez Vieira do;Silva, Rudney da;
Motriz: Revista de Educa??o Física , 2011, DOI: 10.5016/1980-6574.2011v17n2p280
Abstract: the concern with the training of youth in sport has stimulated investigations into the professionalization of coaches. in this sense, the objective of the study was to describe how coaches represent their own professional development in basketball and interpret these representations according to the specialized literature. a qualitative study was conducted with multiple case studies involving four competent coaches of young players in santa catarina. the biographical data were collected with the use of structured and semi-structured interviews. the results show that the coaches give importance to various informal and non-formal sources of development. the observation of other coaches and the experience of daily practice are essential in the construction of professional knowledge. we concluded that the development process of the participating coaches is a result of self-teaching and the socialization of knowledge, which have implied mechanisms of observation, reflection and informal instruction that need to be investigated.
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