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Search Results: 1 - 10 of 200753 matches for " Narang P "
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Isolation and identification of nontuberculous mycobacteria from water and soil in central India
Narang R,Narang P,Mendiratta D
Indian Journal of Medical Microbiology , 2009,
Abstract: Nontuberculous mycobacteria (NTM), important organisms in the Genus Mycobacterium and commonly present in the environment, are known to cause disseminated disease in AIDS patients. In this study, NTM were isolated from environment (soil and water) of the AIDS patients with disseminated NTM disease to know the prevalence of environmental NTM species and their correlation with clinical isolates from patients of the same area. Paraffin baiting technique was used to isolate NTM from environmental samples. Once isolated, subcultures were made on Lowenstein Jensen and Middlebrook 7H10 media and the species were identified using phenotypic and genotypic techniques. A total of 26 NTM isolates belonging to seven different species could be identified. Mycobacterium avium was the only species isolated from both clinical and environmental samples of the same patient; but the isolates did not match using PCR for IS 1311 and IS 1245 spacer sequences.
Evaluation of rapid MTT tube method for detection of drug susceptibility of mycobacterium tuberculosis to rifampicin and isoniazid
Raut U,Narang P,Mendiratta D,Narang R
Indian Journal of Medical Microbiology , 2008,
Abstract: Purpose: To evaluate MTT method for detection of drug resistance to rifampicin and isoniazid in M.tuberculosis . This method utilises the ability of viable mycobacterial cells to reduce MTT( 3-4,5-dimethylthiazol-2-yl-2, 5-diphenyl tetrazolium bromide). Methods: The method was standardised with known resistant and sensitive strains of M.tuberculosis and was then extended to 50 clinical isolates. An inoculum of 10 7 cfu/mL was prepared in Middlebrook 7H9 medium supplemented with oleic acid, albumin, dextrose and catalase. For each drug three tubes were used, one with INH(0.2μg/mL) or RIF(1μg/mL), another as inoculum control and third as blank control. These were incubated at 37°C for four and seven days respectively for RIF and INH after which MTT assay was performed. Results were read visually and by colorimeter at 570 nm. Relative optical density unit (RODU) of 0.2 was taken as cut off. Results were compared with drug sensitivity obtained by proportion method using LJ medium. Results: For rifampicin, concordance with proportion method was 90% by visual and 94% by RODU. Sensitivity and specificity was 86.8% and 100% respectively by visual method and 95.2% and 87.5% respectively by RODU. For Isoniazid, concordance was 94% and sensitivity and specificity was 94.7 and 91.7% respectively by both visual and RODU. Conclusions: MTT assay proved to be rapid and cheap method for performing drug sensitivity of M.tuberculosis
Evaluation of different methods for diagnosis of P. falciparum malaria
Mendiratta D,Bhutada K,Narang R,Narang P
Indian Journal of Medical Microbiology , 2006,
Abstract: Rapid diagnosis is a prerequisite for institution of effective treatment and reducing the mortality and morbidity of falciparum malaria. This study was taken up to compare the efficacy of various rapid methods viz, acridine orange, Plasmodium falciparum histidine rich protein II antigen detection and Field′s stain with traditional microscopy i.e., Leishman stain for diagnosing falciparum malaria. Thick and thin blood films of 443 consecutive patients with history of fever with chills and rigors were examined by Leishman and Field′s method. Acridine orange stained wet mounts of blood were examined under fluorescence microscopy. All films were examined by two independent microbiologists. Plasmodium falciparum histidine rich protein II antigen was detected using commercially available kit, Paracheck Pf. Out of the 443 subjects examined for P.falciparum 18.28% were detected by Leishman stain, 6.32% by Field′s stain, 18.28% by acridine orange and 18.1% by antigen based technique. Field′s stain missed 53 (65.4%), while Paracheck Pf was negative in 6(7.4%) of the Leishman positive samples. All Field′s stain and acridine orange positives were positive by Leishman, but five Paracheck Pf positives were negative. Leishman stain is cost effective but if facilities are available one should use acridine orange for screening. The antigen detection kits are rapid, simple and are useful but to rule out false negatives in clinically suspected cases, Leishman stain is reliable.
Changing patterns of Vibrio cholerae in sevagram between 1990 and 2005
Narang P,Mendiratta D,Deotale V,Narang R
Indian Journal of Medical Microbiology , 2008,
Abstract: Purpose: A retrospective analysis was done to note changes in prevalence, distribution of biotypes, serotypes, antibiotic susceptibility patterns and phage types of Vibrio cholerae isolated in Mahatma Gandhi Institute of Medical Sciences, Sevagram over a period of 16 years. Methods: A total of 535 strains of V. cholerae were isolated from 10,406 stool samples and rectal swabs from January 1990 to December 2005. These comprised of serogroups O1 - 427 (79.89%), O139 - 86 (16.07%) and non O1, non O139 - 22 (4.11%). No classical V. cholerae was isolated. Results: Vibrio cholerae serogroup O1 serotype Ogawa was the predominant isolate till 1992. During 1993, serogroup O139 became the main isolate; however, it completely disappeared during 1995-1996 only to reappear in 1997. Serotype Inaba in our area was conspicuous by its absence with only two strains being isolated till June 1999, but during July-December 1999, 11 out of 15 V. cholerae O1 isolates were El Tor Inaba. T4 was the predominant phage type till 1990, T2 during 1991-1994 and T27 (as per the new scheme) thereafter. Resistance to tetracycline varied between 2 and 17% for V. cholerae O1. Conclusions: The paper reports on the changing epidemiological markers of V. cholerae isolated from a rural hospital over a period of 16 years.
Unusual Presentation of Lupus in Pediatric Patient: Case Report  [PDF]
Karan Raheja, P. S. Narang, Satwinder Kapoor, A. J. Chitkara, Nikhil Vinayak, Alok Kalyani
Open Journal of Rheumatology and Autoimmune Diseases (OJRA) , 2016, DOI: 10.4236/ojra.2016.64016
Abstract: Systemic lupus erythematosus (SLE) is a multisystem disease characterized by loss of self-tolerance resulting in development of autoantibodies and formation of immune complexes. Multiple organ involvement can be seen with renal and neurological involvement carrying the worst prognosis. This case report is of 13-year-old Indian boy who presented with fever and rash, along with Macrophage Activation Syndrome secondary to sepsis. Patient showed improvement in symptoms with steroid therapy and IVIG.
Keratitis due to Colletotrichum dematium - A case report
Mendiratta D,Thamke D,Shukla A,Narang P
Indian Journal of Medical Microbiology , 2005,
Abstract: Colletotrichum dematium has been rarely reproted from India before. The present case, a farmer, developed peripheral corneal ulcer five days following trauma with plant. At presentation his visual acuity was 6/60 (unaided) and 6/24P with pinhole. Slit lamp and fluorescent stain examination revealed paracentral corneal ulcer with irregular margins, stromal infiltration and multiple epithelial defects. Microbiological examination of corneal samples confirmed the initial diagnosis of fungal corneal ulcer and the fungus was identified as C.dematium. Patient was treated with topical natamycin and ciprofloxacin. Patient left against medical advice and was lost to follow up. This report emphasizes that Colletotrichum keratitis may not be rare. Early diagnosis may help in institution of specific therapy early in the disease.
Inducible clindamycin resistance in Staphylococcus aureus isolated from clinical samples
Deotale V,Mendiratta D,Raut U,Narang P
Indian Journal of Medical Microbiology , 2010,
Abstract: Purpose: Clindamycin is commonly used in the treatment of erythromycin resistant Staphylococcus aureus causing skin and soft tissue infections. In vitro routine tests for clindamycin susceptibility may fail to detect inducible clindamycin resistance due to erm genes resulting in treatment failure, thus necessitating the need to detect such resistance by a simple D test on routine basis. Materials and Method: 247 Staphylococcus aureus isolates were subjected to routine antibiotic susceptibility testing including oxacillin (1ìg) by Kirby Bauer disc diffusion method. Inducible clindamycin resistance was detected by D test, as per CLSI guidelines on erythromycin resistant isolates. Results: 36 (14.5%) isolates showed inducible clindamycin resistance, nine (3.6%) showed constitutive resistance while remaining 35 (14.1%) showed MS phenotype. Inducible resistance and MS phenotype were found to be higher in MRSA as compared to MSSA (27.6%, 24.3% and 1.6%, 4% respectively). Conclusion: Study showed that D test should be used as a mandatory method in routine disc diffusion testing to detect inducible clindamycin resistance.
Occurrence of Marek`s Disease in Vaccinated Poultry Flocks of Haryana (India)
Kamaldeep,P.C. Sharma,N. Jindal,G. Narang
International Journal of Poultry Science , 2007,
Abstract: Marek`s Disease (MD), which is a lymphoproliferative disease of chickens, remains one of the most important diseases. In the present study, occurrence of Marek`s disease virus in ten vaccinated poultry flocks of Haryana (India) from different geographical locations was determined on the basis of clinical signs, gross and histopathology and precipitation test. The vaccines used were either HVT alone or in combination with SB-1. Samples from all the flocks had gross and histopathological lesions suggestive of Marek`s disease. Grossly, visceral tumors in liver, spleen, kidney and ovary were observed. The characteristic histopathological lesions included infiltration of pleomorphic lymphoid cells such as lymphoblasts, small and large lymphocytes, plasma cells and occasionally MD cells in different organs. The lesions were non-capsulated and infiltrative type. In immunodiffusion test, feather follicle tip samples from five flocks gave a positive precipitation reaction.
Peritoneal encapsulation of small bowel--(a case report).
Arora P,Narang R,Sethna K,Gupta D
Journal of Postgraduate Medicine , 1989,
Abstract: Peritoneal encapsulation is a rare congenital anomaly. Its occurrence in a female and association with intestinal obstruction are even rarer features which were present in the reported case.
Unusual Maydl′s hernia (a case report).
Narang R,Pathania O,Punjabi P,Tomar S
Journal of Postgraduate Medicine , 1987,
Abstract:
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