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Search Results: 1 - 10 of 31451 matches for " Nan-Shan Chang "
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Transforming growth factor-β1 blocks the enhancement of tumor necrosis factor cytotoxicity by hyaluronidase Hyal-2 in L929 fibroblasts
Nan-Shan Chang
BMC Cell Biology , 2002, DOI: 10.1186/1471-2121-3-8
Abstract: Murine L929 fibroblasts were engineered to stably express green-fluorescent protein (GFP)-tagged hyaluronidase (GFP-Hyal-1 or GFP-Hyal-2) or GFP alone. Compared to control cells, Hyal-2-expressing cells had a significantly increased sensitivity to TNF cytotoxicity (~60–110% increase), while Hyal-1-expressing cells were less sensitive to TNF (~20–90% increase). TNF activated NF-κB, along with IκBα degradation, occurred at 20 to 60 min in Hyal-2 cells post stimulation, but at the 20 min time point in both control and Hyal-1 cells. Hyal-2 cells, but not Hyal-1 and control cells, constitutively expressed WOX1, and transiently expressed Hyal-2 enhanced WOX1-mediated cell death. Unlike PH-20, Hyal-1 and Hyal-2 did not induce p53 expression. Hyal-2 translocated from the lysosome to the mitochondria during staurosporine-mediated apoptosis, suggesting that Hyal-2 may damage mitochondria. Finally, Hyal-1 and Hyal-2 blocked TGF-β1-enhanced L929 cell growth. In contrast, TGF-β1 inhibited Hyal-1- and Hyal-2-increased TNF cytotoxicity in L929 cells by 30–50%.TGF-β1 limits the ability of Hyal-2 to induce TNF cytotoxicity in L929 cells. Hyal-2-increased TNF cytotoxicity in L929 cells appears to be correlated with upregulation of WOX1, a prolonged NF-κB activation, and Hyal-2 translocation to the mitochondria during apoptosis.Transforming growth factor beta (TGF-β) family proteins are multifunctional cytokines capable of regulating cell growth, extracellular matrix protein synthesis, and immune cell functions [1,2]. Hyaluronidase is an extracellular matrix-degrading enzyme. Interestingly, malignant and metastatic breast and prostate cancer cells frequently overexpress both hyaluronidase and TGF-β proteins [3-7]. Previously we have shown that bovine testicular hyaluronidase, also known as PH-20, increases the susceptibility of various cancer cells to tumor necrosis factor (TNF or TNF-α) cytotoxicity [[8], review]. PH-20 induces the expression of proapoptotic p53 [9] and WOX1 (WW doma
Zfra affects TNF-mediated cell death by interacting with death domain protein TRADD and negatively regulates the activation of NF-κB, JNK1, p53 and WOX1 during stress response
Qunying Hong, Li-Jin Hsu, Lori Schultz, Nicole Pratt, Jeffrey Mattison, Nan-Shan Chang
BMC Molecular Biology , 2007, DOI: 10.1186/1471-2199-8-50
Abstract: Transiently overexpressed Zfra caused growth suppression and apoptotic death of many but not all types of cells. Zfra either enhanced or blocked cell death caused by TRADD, FADD, or receptor-interacting protein (RIP) in a dose-related manner. This modulation is related with Zfra binding with TRADD, NF-κB, JNK1 and WOX1, as determined by GST pull-down analysis, co-immunoprecipitation, and mapping by yeast two-hybrid analysis. Functionally, transiently overexpressed Zfra sequestered NF-κB (p65), WOX1, p53 and phospho-ERK (extracellular signal-activated kinase) in the cytoplasm, and TNF or UV light could not effectively induce nuclear translocation of these proteins. Zfra counteracted the apoptotic functions of Tyr33-phosphorylated WOX1 and Ser46-phosphorylated p53. Alteration of Ser8 to Gly abolished the apoptotic function of Zfra and its regulation of WOX1 and p53.In response to TNF, Zfra is upregulated and modulates TNF-mediated cell death via interacting with TRADD, FADD and RIP (death-inducing signaling complex) at the receptor level, and downstream effectors NF-κB, p53, WOX1, and JNK1.Human WWOX/FRA16D gene encodes a candidate tumor suppressor WW domain-containing oxidoreductase, designated WWOX, FOR, or WOX1 [1-3]. This gene is located on a common fragile site ch16q23.3–24.1 [1,2]. Loss of heterozygosity (LOH) of WWOX gene has been found in several types of cancers [[4,5]; reviews]. WWOX/FOR/WOX1 possesses two N-terminal WW domains (containing conserved tryptophan residues), a nuclear localization sequence (NLS) between the WW domains, and a C-terminal short chain alcohol dehydrogenase/reductase (SDR) domain. WWOX mRNA may undergo alternative splicing, thereby generating at least 8 mRNAs mainly coding for proteins with altered SDR domain sequences [4]. Several protein isoforms have been identified [4]. Nonetheless, presence of specific protein isoforms in normal and cancerous tissues remains to be established.WWOX/FOR/WOX1 is considered as a candidate tumor supp
Assessing Current Therapeutic Approaches to Decode Potential Resistance Mechanisms in Glioblastomas
Chun-I Sze,Ming-Fu Chiang,Nan-Shan Chang
Frontiers in Oncology , 2013, DOI: 10.3389/fonc.2013.00059
Abstract: Unique astrocytic cell infiltrating growth and glial tumor growth in the confined skull make human glioblastoma (GBM) one of the most difficult cancers to treat in modern medicine. Prognosis for patients is very poor, as they die more or less within 12 months. Patients either die of the cancer itself, or secondary complications such as cerebral edema, herniations, or hemorrhages. GBMs rarely metastasize to other organs. However, GBM recurrence associated with resistance to therapeutic drugs is common. Patients die shortly after relapse. GBM is indeed an outstanding cancer model to search for potential mechanisms for drug resistance. Here, we reviewed the current cancer biology of gliomas and their pathophysiological events that contribute to the development of therapeutic resistance. We have addressed the potential roles of cancer stem cells, epigenetic modifications, and epithelial mesenchymal transition (EMT) in the development of resistance to inhibitor drugs in GBMs. The potential role of TIAF1 (TGF-β-induced antiapoptotic factor) overexpression and generation of intratumor amyloid fibrils for conferring drug resistance in GBMs is discussed.
Self-aggregating TIAF1 in lung cancer progression
Qunying Hong, Li-Jin Hsu, Ying-Tsen Chou, Chen-Yu Lu, Yu-An Chen, Nan-Shan Chang et al.
Translational Respiratory Medicine , 2013, DOI: 10.1186/2213-0802-1-5
Abstract: Recent studies have demonstrated that transforming growth factor beta (TGF-?1)-induced antiapoptotic factor (TIAF1) is able to form aggregates in the hippocampi of middle-aged normal individuals. The aggregating TIAF1 induces generation of amyloid beta (A?) for causing neurodegeneration. Intriguingly, TIAF1 aggregates are shown, together with Smad4 and A?, in the cancer stroma and peritumor capsules of many solid tumors. During lung cancer progression, for example, TIAF1 and amyloid fibrils are significantly upregulated in the cancer stroma. Aggregates of TIAF1 and A? are shown on the interface between metastatic lung cancer cells and the brain tissues. Conceivably, these peritumor materials are needed for cancer cells to survive. In vitro experiments revealed that TIAF1 is a crucial component for tumor suppressors p53 and WWOX-mediated tumor suppression and apoptosis. While metastatic lung cancer cells are frequently devoid of WWOX and p53, we provide new perspectives regarding the role of TIAF1 in the pathogenesis of lung cancer development, and propose a therapeutic approach for targeting TIAF1.
Xpert MTB/RIF test for rapid diagnosis of Mycobacterium tuberculosis and simultaneous detection of multidrugresistant tuberculous bacillus
Chuang CAI,Nan-shan ZHONG
Medical Journal of Chinese People's Liberation Army , 2012,
Abstract: Tuberculosis (TB) is endemic in China with high prevalence of multiple-drug resistant tuberculous bacilli (MDRTB). The incidence of new cases of TB reaches 1,300 thousand annually. Among them, 5.7 percent are MDR-TB. Staining for acidfast bacilli in sputum and clinicoradiological examination have been the main diagnostic tools for TB, particularly pulmonary TB. However, the positive rate of sputum Ziehl-Neelsen stain for sputum is disappointedly low, merely 28% in newly-diagnosed TB. Moreover, the radiological manifestations of the patients suspected of TB are often non-specific. All these facts call for a simple, accurate and rapid diagnostic method to overcome this bottleneck, which hinders the success of satisfactory TB control in China. Employing both hemi-nested RT-PCR and beacon technology with fluorescent probes, the MTB/RIF diagnostic assay specifically amplifies, thus helps detect the rpoB gene, which is unique to Mycobacterium tuberculosis and also a biomolecularmarker of rifampin resistance. As a semi-quantitative analysis, the quantity of Mycobacterium tuberculosis in samples is reflected by the threshold of PCR cycles during MTB/RIF assay. With Mycobacterium tuberculosis culture as the standard reference, for sputum samples from patients suspected of suffering from pulmonary TB, overall diagnostic sensitivity of MTB/RIF assay is 73.1%–90.0% with a specificity of 99.0%–99.5%. For detection of rpoB gene mutations responsible for rifampin-resistance, the sensitivity is 97.2% and specificity is 98.3%. Following sample loading, the system can automatically complete the diagnostic process and report the results within 2 hours. Targeting the rpoB gene specifically, there is no cross-reaction with non-tuberculosis mycobacteria or other common respiratory pathogens. In addition to sputum samples, the system can be used to detect Mycobacterium tuberculosis in various body fluids (including pleural effusion, urine, cerebrospinal fluid and even bronchoalveolar lavage fluid) and lung tissue biopsy samples. As for sensitivity, the assay is comparable to Mycobacterium tuberculosis culture (for the latter, mean interval between loading and result-reporting is 16 or 30 days (depending upon the culture medium used), and it is 100 times longer than Ziehl-Neelsen stains. Compared with conventional laboratory diagnostic approaches, this assay is much simpler and biohazardous aerosol-free
Complement C1q Activates Tumor Suppressor WWOX to Induce Apoptosis in Prostate Cancer Cells
Qunying Hong, Chun-I Sze, Sing-Ru Lin, Ming-Hui Lee, Ruei-Yu He, Lori Schultz, Jean-Yun Chang, Shean-Jen Chen, Robert J. Boackle, Li-Jin Hsu, Nan-Shan Chang
PLOS ONE , 2009, DOI: 10.1371/journal.pone.0005755
Abstract: Background Tissue exudates contain low levels of serum complement proteins, and their regulatory effects on prostate cancer progression are largely unknown. We examined specific serum complement components in coordinating the activation of tumor suppressors p53 and WWOX (also named FOR or WOX1) and kinases ERK, JNK1 and STAT3 in human prostate DU145 cells. Methodology/Principal Findings DU145 cells were cultured overnight in 1% normal human serum, or in human serum depleted of an indicated complement protein. Under complement C1q- or C6-free conditions, WOX1 and ERK were mainly present in the cytoplasm without phosphorylation, whereas phosphorylated JNK1 was greatly accumulated in the nuclei. Exogenous C1q rapidly restored the WOX1 activation (with Tyr33 phosphorylation) in less than 2 hr. Without serum complement C9, p53 became activated, and hyaluronan (HA) reversed the effect. Under C6-free conditions, HA induced activation of STAT3, an enhancer of metastasis. Notably, exogenous C1q significantly induced apoptosis of WOX1-overexpressing DU145 cells, but not vehicle-expressing cells. A dominant negative and Y33R mutant of WOX1 blocked the apoptotic effect. C1q did not enhance p53-mediated apoptosis. By total internal reflection fluorescence (TIRF) microscopy, it was determined that C1q destabilized adherence of WOX1-expressing DU145 cells by partial detaching and inducing formation of clustered microvilli for focal adhesion particularly in between cells. These cells then underwent shrinkage, membrane blebbing and death. Remarkably, as determined by immunostaining, benign prostatic hyperplasia and prostate cancer were shown to have a significantly reduced expression of tissue C1q, compared to age-matched normal prostate tissues. Conclusions/Significance We conclude that complement C1q may induce apoptosis of prostate cancer cells by activating WOX1 and destabilizing cell adhesion. Downregulation of C1q enhances prostate hyperplasia and cancerous formation due to failure of WOX1 activation.
Macrolide Therapy in Adults and Children with Non-Cystic Fibrosis Bronchiectasis: A Systematic Review and Meta-Analysis
Yong-hua Gao, Wei-jie Guan, Gang Xu, Yan Tang, Yang Gao, Zhi-ya Lin, Zhi-min Lin, Nan-shan Zhong, Rong-chang Chen
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0090047
Abstract: Background A systematic review and meta-analysis was conducted to evaluate the efficacy and safety of macrolide therapy in adults and children with bronchiectasis. Methods We searched the PUBMED, EMBASE, CENTRAL databases to identify relevant studies. Two reviewers evaluated the studies and extracted data independently. The primary outcome was the number of bronchiectasis exacerbations. Secondary outcomes included exacerbation-related admissions, quality of life (QoL), spirometry, 6-minute walk test (6MWT) and adverse events. Results Nine eligible trials with 559 participants were included. Six were conducted on adults, and the remaining on children. Macrolide therapy significantly reduced the number of patients experiencing one or more exacerbation in adults [risk ratio (RR) = 0.59; 95% CI, 0.40 to 0.86; P = 0.006; I2 = 65%] and children [RR = 0.86; 95% CI, 0.75–0.99; P = 0.04; I2 = 0%], but not the number of patients with admissions for exacerbation. Macrolide therapy was also associated with reduced frequency of exacerbations in adults (RR = 0.42; 95% CI, 0.29 to 0.61; P<0.001; I2 = 64%) and children (RR = 0.50; 95% CI, 0.35 to 0.71; P<0.001). Pooled analyses suggested that spirometry, including FEV1 and FVC, were significantly improved in adults but not in children. Macrolide therapy improved the QoL (WMD, ?6.56; 95% CI, ?11.99 to ?1.12; P = 0.02; I2 = 86%) but no significant difference in 6MWT (WMD, 4.15; 95% CI, ?11.83 to 20.13; P = 0.61; I2 = 31%) and the overall adverse events (RR, 0.96; 95% CI, 0.82 to 1.13; P = 0.66; I2 = 0%) in adults. However, reports of diarrhea and abdominal discomforts were higher with macrolide therapy. Conclusions Macrolide maintenance therapy, both in adults and children, was effective and safe in reducing bronchiectasis exacerbations, but not the admissions for exacerbations. In addition, macrolide administration in adults was associated with improvement in QoL and spirometry, but not 6WMT. Future studies are warranted to verify the optimal populations and clarify its potential effects on antimicrobial resistance.
Crude Oil Pipeline Real-Time Security System

XU Nan-Shan,ZHOU Jun,

计算机系统应用 , 2012,
Abstract: In order to solve oil leakage and energy waste problems in current crude oil pipeline transmission,this paper proposes a B/S model based real-time security system for oil pipeline.The system realizes the pipeline data monitoring,pipeline leak detecting,energy-saving model and wax model.The design of the system,database and the main technology of the system are explained in detail in the paper.And with the friendly interface,stable communication,real-time monitoring,and high detection precision,this system really realizes automated monitoring and improves the level of automation of enterprise management.
Method of pagerank planar linear convergence

XU Nan-shan,CONG Lei,

计算机应用 , 2006,
Abstract: In Application, because of the huge number of web pages that even reach to several hundreds of million, it has to spend much time on computing pagerank by power method. So it needs some skills to make the convergence of power method fast. Based on the proof of power method, a method to accelerate the process of computing eigenvector was put forward when using revised power method to calculate the pagerank according to assumption of eigenvector being planar linear expressed. And the new method can shorten time expending meanwhile not increase any space storage. At last test data shows this method which comes from theory gets good convergence result in practice.
Dramatic Co-Activation of WWOX/WOX1 with CREB and NF-κB in Delayed Loss of Small Dorsal Root Ganglion Neurons upon Sciatic Nerve Transection in Rats
Meng-Yen Li,Feng-Jie Lai,Li-Jin Hsu,Chen-Peng Lo,Ching-Li Cheng,Sing-Ru Lin,Ming-Hui Lee,Jean-Yun Chang,Dudekula Subhan,Ming-Shu Tsai,Chun-I Sze,Subbiah Pugazhenthi,Nan-Shan Chang,Shur-Tzu Chen
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0007820
Abstract: Tumor suppressor WOX1 (also named WWOX or FOR) is known to participate in neuronal apoptosis in vivo. Here, we investigated the functional role of WOX1 and transcription factors in the delayed loss of axotomized neurons in dorsal root ganglia (DRG) in rats.
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