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Search Results: 1 - 10 of 4716 matches for " NI LUH PUTU INDI DHARMAYANTI "
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Molecular Analysis of H5N1 Avian Influenza Virus from Avian Species: Compared with Genbank Data of the Indonesian H5N1 Human Cases
NI LUH PUTU INDI DHARMAYANTI
Microbiology Indonesia , 2009,
Abstract: In Indonesia, the H5N1 avian influenza (AI) disease has been circulating for more than five years and has infected various types of avian species and human beings. Generally, avian influenza cases in human beings are suspected to be spread by chicken, birds or waterfowl previously infected by avian influenza. The data supporting this assumption were very limited, therefore the molecular characterization on four avian influenza genome segments such as hemagglutinin, neuraminidase, matrix and non structural that was isolated from the avian species surrounding the avian influenza cases in human was conducted. The analysis was conducted on these genes which were responsible for binding receptors, the pathogenicities, and the resistance to antiviral drugs, thus the virus changes can be detected by comparing the sequence data of GenBank from human cases related to the avian species. The four avian influenza viruses used in this study isolated from avian influenza cases surrounding the avian influenza cases in human in 2007. The results of genetic analysis showed that these four viruses and the available sequence data from the GenBank for of avian influenza virus in human and avian have the receptor a-2,3 of sialic acid which is the avian receptor. The A/Ck/West Java/Bks2/2007 virus is collected from the chicken surrounding the avian influenza cases in human that resembles the data of avian influenza virus from human, A/Indonesia/CDC1031/2007 from GenBank. The viruses conferred similarities amino acid sequence of hemagglutinin, neuraminidase, matrix and non structural protein. All viruses used have deletion at the position 80-84 of the NS1 protein and possessed the ESEV motif which may contribute to an increased virulence. The avian influenzaviruses examined in this study also show resistance to amantadine
Influenza H5N1 Virus of Birds Surrounding H5N1 Human Cases Have Specific Characteristics on the Matrix Protein
NI LUH PUTU INDI DHARMAYANTI,FERA IBRAHIM,DARMINTO,AMIN SOEBANDRIO
HAYATI Journal of Biosciences , 2011,
Abstract: The H5N1 influenza virus in Indonesia has caused more than 100 people died due to the virus infections. Cases in humans were mostly due to the virus spread from the infected birds. This study characterized molecularly the H5N1 virus from birds around the H5N1 infection cases in humans in Indonesia. Result from this study revealed that in several cases, waterfowl species could become the source of H5N1 infections in human. We found that the one of six viruses used in this study probably was a first antigenic shift virus in Indonesia. This study shows that the AI viruses isolated from birds around humans infected by H5N1 virus has specific characteristics namely the presence of several amino acid substitutions especially on the M1 and M2 proteins. The substitutions are similar in most of H5N1 human cases in Indonesia.
Amantadine Resistant of Indonesian H5N1 Subtype Influenza Viruses During 2003-2008
NI LUH PUTU INDI DHARMAYANTI,FERA IBRAHIM,AMIN SOEBANDRIO
Microbiology Indonesia , 2010, DOI: 10.5454/mi.4.1.3
Abstract: The M2 protein of 146 avian influenza (AI) viruses data available in public database (NCBI), including 20 AI isolates used in this study, were sequenced at the M2 protein to find out the probability of mutation and the increase of resistance to amantadine after more than 5 years of their circulation in Indonesia. The results showed that during 2003-2008, around 62.58% (92/147) AI viruses in Indonesia have showed resistance to amantadine and 10 of them have dual mutations at V27A and S31N.
Expression of Two N1 Clones with Single Amino Acid Dissimilarity of Avian Influenza H5N1 Virus
RISZA HARTAWAN,NI LUH PUTU INDI DHARMAYANTI,KARL ROBINSON,TIMOTHY MAHONY
HAYATI Journal of Biosciences , 2012,
Abstract: Two clones of N1 gene derived from isolate A/Dk/Tangerang/Bbalitvet-ACIAR-TE11/2007 (H5N1) exhibit single mismatch of amino acid sequence at position 242 that is threonine and methionine for the clone #3 and #5, respectively. In order to evaluate the effect of the amino acid substitution, these clones were inserted into two different expression vectors that are pEGFP-C1 and pcDNA-3.3 TOPO TA cloning. Subsequently, the respective recombinant clones were transfected into eukaryotic cells, including CEF, RK13 and VERO using Lipofectamine ‘plus’ reagent. As a result, the clone #3 retaining atypical sequence showed lower expression level rather than the clone #15 in both vectors and all type of cells. The 3D conformational modelling revealed that the mutation occurs in the inner part of glycoprotein embedded within envelope or matrix. Therefore, the missense mutation seems has no effect on the antigenic properties of neuraminidase but this substitution by any means causes lethal mutagenesis in the individual gene expression by reducing level of protein transcript.
The Genetic Drift of Indonesian Avian Influenza A H5N1 Viruses During 2003-2008
NI LUH PUTU INDI DHARMAYANTI,GINA SAMAAN,FERA IBRAHIM,RISA INDRIANI
Microbiology Indonesia , 2011, DOI: 10.5454/mi.5.2.4
Abstract: The avian influenza A H5N1 outbreaks started in 2003 and Indonesia introduced a vaccination campaign in 2004 to control the disease. In 2007, anecdotal reports about reduced vaccine effectiveness were received from commercial farmers. This paper describes the evolution of viruses in Indonesia up till 2008 and focus on viruses from vaccinating farms reporting vaccine failure were compared to viruses isolated from outbreak areas with no vaccination program. Result of the study revealed that viruses from vaccinated chickens had more extensive mutation at the HA molecule compared to chicken and other avian species without vaccination. Substitutions occurred at the HA gene level as well as at NA, M1 and NS1 genes. Viruses isolated and characterized form 2008 vaccinated flocks had substitutions that were unique and different with the old viruses. The recommendation arising from this study to the avian influenza disease control program in Indonesia is that continuous monitoring of genetic character of viruses and the vaccine seed strain should be updated periodically and matched with the virus circulated in the field.
Profitabilitas Strategi Investasi Kontrarian di Bursa Efek Indonesia
Ni Luh Putu Wiagustini
Jurnal Manajemen dan Kewirausahaan , 2008,
Abstract: This objectives of this research are investigate the stock portfolio performance with high price earning ratio (PER) and low PER following ownership period compared to the portfolio performance of the formation period; to investigate the stock portfolio performance of high PER compared to that of low PER; and to investigate the stock portfolio performance of high PER and low PER stock compared to market portfolio return. The sample used in this research is the go-public companies whose stocks are listed at Indonesian Stock Exchange. The finding of the research shows that the high PER stock portfolio performance of the following period is desreasing and lower than the low PER stock portfolio performance and market portfolio return; on the other hand, the low PER stock portfolio of the following period is increasing and higher than the high PER stock portfolio performance and market portfolio return. The research finding showing that the stocks possessing either high PER or low PER at the beginnings experiences return reversal at the following period indicates market anomaly related to the overreaction hyphotesis “PER Effect”. Then this popularizes the contrarian investment strategy in Indonesian Stock Exchange. Abstrac in Bahasa Indoensia: Penelitian ini bertujuan untuk menguji kinerja portofolio saham price earning ratio (PER) tinggi dan PER rendah periode kepemilikan berikutnya dibandingkan dengan kinerja portofolio saham periode formasinya, menguji kinerja portofolio saham PER tinggi dibandingkan dengan PER rendah, dan menguji kinerja portofolio saham PER tinggi dan PER rendah dibandingkan dengan return portofolio pasar. Hasil penelitian menunjukkan bahwa, kinerja portofolio saham PER tinggi pada periode berikutnya menurun dan lebih rendah dibandingkan dengan kinerja portofolio saham PER rendah dan return portofolio pasar; sebaliknya kinerja portofolio saham PER rendah pada periode berikutnya meningkat dan lebih tinggi dibandingkan dengan kinerja portofolio saham PER tinggi dan return portofolio pasar. Temuan yang menunjukkan saham-saham yang pada mulanya memiliki PER tinggi atau PER rendah mengalami pembalikan return pada periode berikutnya, mengindikasikan terjadi anomali pasar yang berkaitan dengan hipotesis overreaction “Efek PEER. Sehingga ini mempopulerkan strategi investasi kontrarian di Bursa Efek Indonesia. Kata kunci: kinerja portofolio, return portofolio pasar dan anomali pasar.
UJI TOKSISITAS JAMUR METARHIZIUM ANISOPLIAE TERHADAPLARVA NYAMUK AEDES AEGYPTI
Ni Luh Putu Manik Widiyanti,Sanusi Muyadihardja
Media of Health Research and Development , 2012,
Abstract: Beside being the vector for dengue fever, Aedes aegypti also disturbs human life since the female bites and absorbs blood particularly in the morning between 08.00 - 12.00 and in the afternoon between 15.00-17.00 A lot of efforts have been made to solve dengue fever problems. These include chemical method, physical method and biological control. So far mosquito control still emphasizes chemical method, that is by using insecticide. Repeated use of insecticide causes a new problem, namely, it kills non target insect and vector resistance. The negative effect caused by chemical or insecticide encouraged scientists to find an alternative method, that is biological control. One of the methods that given priority to be developed as a controlling agent is entomophatogenic Metarhizium anisopliae fungus because it has destruxins that has been considered to be a new generation of insecticide substance. This study aimed at finding out the toxicity of M. anisopliae to Aedes aegypti mosquito larvae by determining its Lethal Concentration (LC) at 50 and 90. In addition, it also aimed at finding out the number of M. anisopliae conidiosphore at the respective concentrations. The result of the study shows that entomophatogen kills 50% (LC50) larvae instar III Ae aegypti that comes from Denpasar at the 2.955 x 10-2 dilution level in 200 ml of water and 90% (LC90) at the 8.861 x 10-1 dilution level in 200 ml of water in laboratory condition. There is a significant difference in the number of conidia and the entomophatogenic lethal power at each concentration. Key words : biological control, Metarhizium anisopliae, Aedes aegypti
Young Indonesian Cruise Workers, Symbolic Violence and International Class Relations
Luh Putu Artini,Pam Nilan,Steven Threadgold
Asian Social Science , 2011, DOI: 10.5539/ass.v7n6p3
Abstract: This article reports on an investigation of the experiences of well-educated young Indonesians who choose a career in cruise ship work rather than go to university, or work in the local tourist industry. A sociological approach is taken that sees the cruise ship environment as a microcosm of international class relations. Attention is paid to the symbolic violence experienced by young workers who find their dream of travel on the high seas consists of mundane service work in uncomfortable conditions far from home. The phenomenon suggests the emergence of a young cosmopolitan migrant worker who does not experience any acculturation to a new country, but acculturation to life in a “total environment” of 24 hour service work subject to few international labor regulations. Undoubtedly there are financial and work experience benefits, but there is also much to be endured in the extended transition from youth to adulthood during cruise ship work.
EVALUASI GALUR JAGUNG SMB-5 HASIL SELEKSI MASSA VARIETAS LOKAL BALI ”BERTE” PADA DAERAH KERING
Ni Luh Made Pradnyawathi
Bumi Lestari , 2012,
Abstract: TThe aimed of this study was to know agronomic characters performance of SMB-5 line mainly yield potential, compared to another local varieties in an arid area. The study was conducted at Sumber Klampok Buleleng in 2007. Randomized Block Design was used, seven varieties i.e. : SMB-5 line (Bt), Seraya (Sr), Cicih Tombong (Ct), Pulut Putih (Pp), Lombok Putih (Lp), Tongtongan Tianyar (Tt), and Ketokong (Kt) as a treatment with three replications. Ninety plants were taken as a random sampling from every genotype. Quantitative characters of SMB-5 line and another varieties tested showed a significant different for more variables except for maximum leaf number, time of tasseling, time of silking, number of productive pods and harvest index. SMB-5 line and another varieties tested was different in genetics showed by a high heritability (h2 > 0.50), and some others showed a main heritability (h2 >0.20). SMB-5 give the highest yield (4.02 ton/ha) which are significant different from another local varieties. SMB-5 lines have a good adaptation potential to an arid area.
PENGARUH FLUKTUASI AIR TANAH TERHADAP KANDUNGAN BAHAN KIMIA SKUNDER DAN DIVERSITAS INSEK PADA TANAMAN MELALEUCA ERICIFOLIA SM.
Ni Luh Watiniasih
Bumi Lestari , 2010,
Abstract: Fluctuation of water regimes where plant grows has been known to affect the plant chemical, nutrition compositions and plant chemical defenses, which can affect the vulnerability of plants to insect herbivores. This research aimed to compare the concentration of plant nutrition and chemical defenses of Melaleuca ercifolia grows on different water regimes, subsequently to insect herbivore attacks. The leaf total nitrogen was higher in plants growing in water fluctuated areas, therefore the insect herbivores compared to plants growing in prolong-flooded condition. Insect herbivores prefer to consume plants that are more nutritious and less affected by the plant growing conditions.
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