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Search Results: 1 - 10 of 586015 matches for " N.A. Siddique "
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Collection of Indigenous Knowledge and Identification of Endangered Medicinal Plants by Questionnaire Survey in Barind Tract of Bangladesh
N.A. Siddique,M.A. Bari,A.T.M. Naderuzzaman,N. Khatun
Journal of Biological Sciences , 2004,
Abstract: This paper deals with over one hundred plants of ethnobotanical importance, used by the village doctors especially village kaviraj of Barind Tract for medicinal purposes.This work aims at the preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines and also identification of endangered medicinal plants by questionnaire survey.The data presented in this paper has been collected by personal contact with the local people and herbal practitioners, obtaining the information with great difficulty because of their recticence in divulging the secret of the identity of plants of great traditional reputation.
Plant Regeneration from Axillary Shoots Derived Callus in Aristolochia indica Linn. an Endangered Medicinal Plant in Bangladesh
N.A. Siddique,M.A. Bari,M.M. Pervin,N. Nahar
Pakistan Journal of Biological Sciences , 2006,
Abstract: A procedure for rapid in vitro propagation of the aromatic and medicinal plant Aristolochia indica Linn. (Family: Aristolochiaceae) from axillary shoots is described. The highest percentage of callus induction was (95.00) on Murashige and Skoog (15) (MS) medium supplemented with 2.0 mg L-1 Kn and 1.0 mg L-1 BAP. Colour of the calli were mostly light green to dark green. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented with BAP and Kn alone or in BAP combination with NAA and IAA or NAA, IAA and BAP in combination with Kn. The Highest percentage (95.00) of shoot regeneration was obtained in MS medium fortified with 2.5 mg L-1 Kn and 1.0 mg L-1 BAP. The elongated shoots developed roots on a medium containing 1 mg L-1 Kn. The rooted plants were transferred to soil.
Induction of Haploid Rice Plants Through in vitro Anther Culture
Sharmin Shahnewaz,M.A. Bari,N.A. Siddique,N. Khatun
Pakistan Journal of Biological Sciences , 2003,
Abstract: Anthers of three rice cultivars having late uninucleate microspores were studied for induction of haploid rice plants. Anthers were cultured on Z2 medium supplemented with various combinations and concentrations of auxins and kinetin (cytokinin). The best callusing from cultured anthers obtained in 2 medium containing 2,4-D 2 mg l-1, NAA 2.5 mg l-1 and kin 0.5 mg l-1. For regeneration of haploid plantlets anther derived calli were transferred to modified MS medium enriched with kin 1 mg l-1, NAA 1 mg l-1, BAP 1.0 ml l-1 and incubated at 25 1OC in light. BRRI Dhan-29 produced both green and albino plants while BR-3 produced green plants only. Green plantlets obtained from BRRI Dhan-29 were examined and found to be 69.2% haploid plants.
Screeningof Endangered Medicinal Plants Species by Questionnaire Survey in Barind Tract in Bangladesh
N.A. Siddique,M.A. Bari,M.M. Pervin,N. Nahar
Pakistan Journal of Biological Sciences , 2005,
Abstract: This study deals with over two hundred medicinal plants of ethnobotanical importance, used by the village doctors especially village Kavirajs in Barind Tract for medicinal purposes. This study aims at the identification of endangered medicinal plants by questionnaire survey and also preservation and perpetuation of this knowledge of the local plants possessing medicinal properties for the benefit and further fruitful investigation on modern scientific lines.
Plant Regeneration from Nodal Segments Derived Callus in Hemidesmus indicus (L.) R. Br (Anantamul) an Endangered Medicinal Plant in Bangladesh
N.A. Siddique,M.A. Bari,N. Khatun,M. Rahman
Journal of Biological Sciences , 2003,
Abstract: Callus cultures were initiated from axillary shoots on Murashige and Skoog (MS) (1962) medium supplemented with 2,4-D, NAA, and Kn. The highest frequency (85.00%) of organogenic callus induction was observed in MS medium containing 1.0 mg l G 1 NAA and 2.0 mg l G 1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP, NAA, IAA and Kn. Shoots differentiated best (86.00%) from axillary shoot derived callus on MS medium containing 1.0 mg l G 1 NAA and 2.5 mg l G 1 Kn. Regenerated shoots rooted best in MS supplemented in the IBA and Kn. Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
Regeneration of Shoot from Cotyledon Derived Callus of Chickpea (Cicer arietinum L.)
S. Huda,N.A. Siddique,N. Khatun,M.H. Rahman
Pakistan Journal of Biological Sciences , 2003,
Abstract: Regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of chickpea (Cicer arietinum L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (95) of callus formation was observed on MS+ 3.0 mg l ̄1 2, 4-D+3.0 mg l ̄1 BAP. The maximum percentage (40) of shoot bud formation was obtained on MS medium fortified with 2.0 mg l ̄1 BAP and 0.5 mg l ̄1 NAA with number of shoots per callus was 2.50. The regenerated shoots developed highest percentage (77) roots on MS basal medium containing 1.0 mg l ̄1 IBA. Regenerated plants were successfully established in soil after acclimatization. Maximum survivability after 4 weeks of transplantation was achieved in 21 days old rooted shoots on garden soil.
Temporal Variation of Atmospheric Aerosol Particulate Matters and Heavy Metal Concentrations in Dhaka, Bangladesh
Tanzir Al Mahmud, , and *,M. N.A. Siddique,Abdus Salam,A. M. Shafiqul Alam
Pakistan Journal of Analytical and Environmental Chemistry , 2008,
Abstract: Aerosol particulate matters and heavy metal concentrations were measured for the period of August and September 2005 in Dhaka a Southeast Asian Mega City. Particulate matters (PM) of different size fractions (TSP, PM10 and PM2.5) were collected on the micro fiber filters by placing the PM sampler on the roof of the Mohkarram Hossain Khundhkar Biggan Bhaban at the Department of Chemistry, University of Dhaka, Bangladesh. The overall average concentrations of TSP, PM10 and PM2.5 were 68, 43 and 35 μg m-3, respectively. About 82% particles were from fine fraction (PM2.5) and 18% were from coarse fraction (PM10-2.5), which indicates mechanical processes are one of the main sources for the particulate matters in Dhaka. Heavy metals (lead, copper, zinc, and iron) concentrations were determined by using atomic absorption spectrophotometer (AAS) for the size fraction of PM10 with highest concentrations recorded for iron (2360 ng m-3) and lowest for copper (28 ng m-3). The average concentration for lead (96 ng m-3) was lower than the WHO guideline value and also lower than the previous measurements in Dhaka. The lower concentration of lead was found presumably due to the official ban of leaded gasoline in Dhaka, Bangladesh.
Effects of Genotype on Induction of Callus and Plant Regeneration Potential in vitro Anther Culture of Rice (Oryza sativa L.)
Sharmin Shahnewaz,M.A. Bari,N.A. Siddique,M.H. Rahman
Pakistan Journal of Biological Sciences , 2004,
Abstract: The present studies were carried out to evaluate the response of anthers of six rice genotypes to callus induction and plant regeneration. For callus induction Z2 media supplemented with 2, 4-D 0.5 mg l-1, NAA 2.5 mg l-1 and Kinetin 0.5 mg l- was used. Of the six genotypes, five gave good callusing response. Highest callus induction was observed in BRRI Dhan-29 (8.06%) and lowest in BR-10 (1.42%). Callus induction frequency varied from 1.42-8.06% depending on genotypes. Modified MS medium supplemented with Kinetin 0.5 mg l-1, BAP 2 mg l-1 and NAA 1.0 mg l-1 was used for plant regeneration. The results showed a significant genotypic difference in callus induction and plant regeneration. The regeneration of plantlet from anther derived calli ranged from 57-75% conclusion.
Plant Regeneration of Withania somnifera (L.) Dunal (Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant in Bangladesh
N.A. Siddique,M.A. Bari,Sharmin Shahnewaz,M.H. Rahman
Journal of Biological Sciences , 2004,
Abstract: Callus cultures were initiated from nodal segments on Murashige and Skoog[19] medium supplemented with 2,4-D, BAP and Kn. The highest frequency (85%) of organogenic callus induction was observed in MS medium containing 1 mg L ha-1 BAP and 2 mg L ha-1 Kn. Development of adventitious shoots occurred when the calli were subcultured in MS medium supplemented in the BAP and Kn. Shoots differentiated best (80%) from node derived callus on MS medium containing 1 mg L ha-1 BAP and 2.5 mg L ha-1 Kn. Regenerated shoots rooted best on MS medium containing IBA and Kn (1 mg L ha-1 ). Plantlets were transferred to pots containing sand and soil mixture, acclimatized in a culture room and finally rooted plants were transferred to soil.
Micropropagation of Woody Legume (Albizia lebbeck) Through Tissue Culture
A.N.K. Mamun,M.N. Matin,M.A. Bari,N.A. Siddique
Pakistan Journal of Biological Sciences , 2004,
Abstract: The woody legume, Albizia lebbeck, commonly cultivated for social forestry in rural area of Bangladesh was selected for micropropagation by tissue culture technique. Cotyledon, nodal segment of in vitro grown seedlings and nodal segments of field grown mature tree were used as explants. With proper manipulation of cytokinin and auxin combinations and concentrations, it was possible to induce callus from three types of explants of Albizia lebbeck. Different combinations of BA with NAA and KIN with NAA were proved efficient media formulation for callus induction. Among all the hormonal supplements used BA-NAA combination with MS medium was proved best in all respect of callusing response in Albizia lebbeck. Among the explants the in vitro internodal segments were the best for callus induction. The highest 100% callus induction was observed in media having MS+2.0 mg L-1 BA+0.2 mg L-1 NAA and the fresh weight of callus was 1.3 g. Among these three explants of Albizia lebbeck only calli derived from cotyledon regenerated best shoots in MS media supplemented with BA singly or in combination with NAA and KIN. In respect of direct shoot regeneration nodal explants of Albizia lebbeck produced highest range of regenerated shoots.
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