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Search Results: 1 - 10 of 20705 matches for " Myoung Sook Kim "
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Nucleolin Binds to the Proliferating Cell Nuclear Antigen and Inhibits Nucleotide Excision Repair
Chonglin Yang,Myoung Sook Kim,Devulapalli Chakravarty,Fred E. Indig
Molecular and Cellular Pharmacology , 2009,
Abstract: Nucleolin is over-expressed in malignant tumors and is used as a marker for cell proliferation and to reliably predict tumor growth rate. However, it is not known whether nucleolin expression is directly involved in or is a consequence of carcinogenesis. Using GST-pull down assays, we have determined that the recombinant nucleolin interacts with the Proliferating Cell Nuclear Antigen (PCNA). Co-immunoprecipitation assays indicate that the nucleolin-PCNA interaction also occurs in intact cells and this interaction increases after exposure of colon carcinoma RKO cells to UV radiation. Moreover, our data indicate that PCNA and nucleolin co-localize in some areas within the RKO cell nuclei. The functional significance of this interaction is evaluated on Nucleotide Excision Repair (NER) since PCNA is a primary mediator of this cellular function. Our data indicate that overexpression of nucleolin decreases the repair efficiency of UV damaged plasmid DNA in RKO cells. Co-transfection with PCNA can rescue this effect in vivo. Furthermore, reduction of nucleolin protein levels increases DNA repair efficiency in RKO and CHO cells and consequently increases cell survival. These data indicate that the direct interaction of nucleolin with PCNA inhibits NER efficiency of UV damaged DNA. This effect could contribute to carcinogenesis and aging in cells over-expressing nucleolin.
A Novel Pathogenicity Gene Is Required in the Rice Blast Fungus to Suppress the Basal Defenses of the Host
Myoung-Hwan Chi,Sook-Young Park,Soonok Kim,Yong-Hwan Lee
PLOS Pathogens , 2009, DOI: 10.1371/journal.ppat.1000401
Abstract: For successful colonization and further reproduction in host plants, pathogens need to overcome the innate defenses of the plant. We demonstrate that a novel pathogenicity gene, DES1, in Magnaporthe oryzae regulates counter-defenses against host basal resistance. The DES1 gene was identified by screening for pathogenicity-defective mutants in a T-DNA insertional mutant library. Bioinformatic analysis revealed that this gene encodes a serine-rich protein that has unknown biochemical properties, and its homologs are strictly conserved in filamentous Ascomycetes. Targeted gene deletion of DES1 had no apparent effect on developmental morphogenesis, including vegetative growth, conidial germination, appressorium formation, and appressorium-mediated penetration. Conidial size of the mutant became smaller than that of the wild type, but the mutant displayed no defects on cell wall integrity. The Δdes1 mutant was hypersensitive to exogenous oxidative stress and the activity and transcription level of extracellular enzymes including peroxidases and laccases were severely decreased in the mutant. In addition, ferrous ion leakage was observed in the Δdes1 mutant. In the interaction with a susceptible rice cultivar, rice cells inoculated with the Δdes1 mutant exhibited strong defense responses accompanied by brown granules in primary infected cells, the accumulation of reactive oxygen species (ROS), the generation of autofluorescent materials, and PR gene induction in neighboring tissues. The Δdes1 mutant displayed a significant reduction in infectious hyphal extension, which caused a decrease in pathogenicity. Notably, the suppression of ROS generation by treatment with diphenyleneiodonium (DPI), an inhibitor of NADPH oxidases, resulted in a significant reduction in the defense responses in plant tissues challenged with the Δdes1 mutant. Furthermore, the Δdes1 mutant recovered its normal infectious growth in DPI-treated plant tissues. These results suggest that DES1 functions as a novel pathogenicity gene that regulates the activity of fungal proteins, compromising ROS-mediated plant defense.
Operational Implementation of the ATOVS Processing Procedure in KMA and Its Validation
Myoung-Hwan AHN,Mee-Ja KIM,Chu-Yong CHUNG,Ae-Sook SUH,
Myoung-Hwan AHN
,Mee-Ja KIM,Chu-Yong CHUNG,Ae-Sook SUH

大气科学进展 , 2003,
Abstract: The Korea Meteorological Administration (KMA) has processed the data from the advanced TOVS (ATOVS) onboard NOAA-16 satellite since May 2001. The operational production utilizes the AAPP (ATOVS and AVHRR Processing Package) of EUMETSAT and IAPP (International ATOVS Processing Package) of the University of Wisconsin. For the initial guess profiles, the predicted fields (usually 6 to 12 hour forecasted fields) from the global aviation model of NOAA/NCEP are used. The average number of profiles retrieved from the ATOVS data is about 1,300 for each morning and afternoon orbit at about 18 and 06 UTC, respectively. The retrieved temperature and dew point temperatures are provided to forecasters in real time and used for initialization of prediction models. With the advanced microwave sensor (AMSU; Advanced Microwave Sounding Unit), accuracy of the ATOVS products is expected to be better than that of the TOVS products, especially in cloudy conditions. Indeed, the preliminary results from a validation study with the collocated radiosonde data during a 8-month period, from May to December 2001, for the East Asia region show an improved accuracy of the ATOVS products for cloudy skies versus the TOVS, especially for higher altitudes. The RMS (Root Mean Square) difference between the ATOVS products and radiosonde data is about 1.3°C for both clear and cloudy conditions, except for near the ground and at higher altitudes, at around 200 hPa. There is no significant temporal variation of the error statistics at all pressure levels. In case of the water vapor mixing ratio, the largest difference is shown at lower altitudes, while the accuracy is much better for the clear sky cases than the cloudy sky cases. The bias and RMSE at lower altitudes is about 0.557 g kg 1 and 2.5 g kg 1 and decrease significantly with increasing altitude.
The Impact of Diversity Climate for Women on Female Employees’ Intentions to Stay: A Cross-Cultural Study on South Korea and Norway  [PDF]
Hyo-Sook Kim
American Journal of Industrial and Business Management (AJIBM) , 2017, DOI: 10.4236/ajibm.2017.71001
Abstract: The purpose of this research was to examine what factors influence female workers’ intentions to stay with their employer. The effect of diversity climate for women on female employees’ intentions to stay was investigated. Also, based on compensation theory, this study hypothesized the moderation effect of a societal culture, especially Hofstede’s masculinity-femininity dimension, on the association between diversity climate for women and female employees’ intentions to stay. An experiment was conducted to test the hypotheses of this research using South Korea and Norway as study venues. The results revealed that there was a positive association between diversity climate for women and female employees’ intentions to stay. Moreover, in the investigation of societal masculinity’s moderating role, it was shown that diversity climate for women was highly influential in strengthening the intent to stay for female employees from a masculine society. On the other hand, for those from a feminine society, the impact of diversity climate for women was not as strong as for those from a masculine society. By clarifying the role that a societal culture played in female workers’ intentions to stay with their employing organization, the results of this study emphasized the importance of supportive contexts not only at work, but also within a society.
Neurofilament Heavy Polypeptide Regulates the Akt-β-Catenin Pathway in Human Esophageal Squamous Cell Carcinoma
Myoung Sook Kim,Xiaofei Chang,Cynthia LeBron,Jatin K. Nagpal,Juna Lee,Yiping Huang,Keishi Yamashita,Barry Trink,Edward A. Ratovitski,David Sidransky
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009003
Abstract: Aerobic glycolysis and mitochondrial dysfunction are common features of aggressive cancer growth. We observed promoter methylation and loss of expression in neurofilament heavy polypeptide (NEFH) in a significant proportion of primary esophageal squamous cell carcinoma (ESCC) samples that were of a high tumor grade and advanced stage. RNA interference-mediated knockdown of NEFH accelerated ESCC cell growth in culture and increased tumorigenicity in vivo, whereas forced expression of NEFH significantly inhibited cell growth and colony formation. Loss of NEFH caused up-regulation of pyruvate kinase-M2 type and down-regulation of pyruvate dehydrogenase, via activation of the Akt/β-catenin pathway, resulting in enhanced aerobic glycolysis and mitochondrial dysfunction. The acceleration of glycolysis and mitochondrial dysfunction in NEFH-knockdown cells was suppressed in the absence of β-catenin expression, and was decreased by the treatment of 2-Deoxyglucose, a glycolytic inhibitor, or API-2, an Akt inhibitor. Loss of NEFH activates the Akt/β-catenin pathway and increases glycolysis and mitochondrial dysfunction. Cancer cells with methylated NEFH can be targeted for destruction with specific inhibitors of deregulated downstream pathways.
Cysteine Dioxygenase 1 Is a Tumor Suppressor Gene Silenced by Promoter Methylation in Multiple Human Cancers
Mariana Brait, Shizhang Ling, Jatin K. Nagpal, Xiaofei Chang, Hannah Lui Park, Juna Lee, Jun Okamura, Keishi Yamashita, David Sidransky, Myoung Sook Kim
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0044951
Abstract: The human cysteine dioxygenase 1 (CDO1) gene is a non-heme structured, iron-containing metalloenzyme involved in the conversion of cysteine to cysteine sulfinate, and plays a key role in taurine biosynthesis. In our search for novel methylated gene promoters, we have analyzed differential RNA expression profiles of colorectal cancer (CRC) cell lines with or without treatment of 5-aza-2′-deoxycytidine. Among the genes identified, the CDO1 promoter was found to be differentially methylated in primary CRC tissues with high frequency compared to normal colon tissues. In addition, a statistically significant difference in the frequency of CDO1 promoter methylation was observed between primary normal and tumor tissues derived from breast, esophagus, lung, bladder and stomach. Downregulation of CDO1 mRNA and protein levels were observed in cancer cell lines and tumors derived from these tissue types. Expression of CDO1 was tightly controlled by promoter methylation, suggesting that promoter methylation and silencing of CDO1 may be a common event in human carcinogenesis. Moreover, forced expression of full-length CDO1 in human cancer cells markedly decreased the tumor cell growth in an in vitro cell culture and/or an in vivo mouse model, whereas knockdown of CDO1 increased cell growth in culture. Our data implicate CDO1 as a novel tumor suppressor gene and a potentially valuable molecular marker for human cancer.
Korea’s Construction Business Informatization Overview and Future Plans  [PDF]
Myoung Bae Seo, Nam Gon Kim
Creative Education (CE) , 2012, DOI: 10.4236/ce.2012.37B003
Abstract: South Korea’s construction industry won orders worth USD 58 billion in 2011, and as such, it has achieved a remarkable growth. However, despite such an impressive quantitative growth, value-added quality growth has been very slow. Thus, the Ministry of Land, Transport and Marine Affairs (MLTM) is pushing ahead with the construction informatization initiative (Construction Continuous Acquisition & Life-cycle Support - CALS) in order to systematically manage a wide range of construction information by stage and to enhance the efficiency of construction costs. This study aims to review the country’s construction CALS to explore a construction development direction. 
Expression of Aquaporin 5 (AQP5) Promotes Tumor Invasion in Human Non Small Cell Lung Cancer
Young Kwang Chae, Janghee Woo, Mi-Jung Kim, Sung Koo Kang, Myoung Sook Kim, Juna Lee, Seung Koo Lee, Gyungyub Gong, Yong Hee Kim, Jean Charles Soria, Se Jin Jang, David Sidransky, Chulso Moon
PLOS ONE , 2008, DOI: 10.1371/journal.pone.0002162
Abstract: The aquaporins (AQP) are water channel proteins playing a major role in transcellular and transepithelial water movement. Recently, the role of AQPs in human carcinogenesis has become an area of great interest. Here, by immunohistochemistry (IHC), we have found an expression of AQP5 protein in 35.3% (IHC-score: ≥1, 144/408) of the resected NSCLC tissue samples. Cases with AQP5-positive status (IHC-score: ≥2) displayed a higher rate of tumor recurrence than negative ones in NSCLC (54.7% vs. 35.1%, p = 0.005) and worse disease-free survival (p = 0.033; OR = 1.52; 95%CI:1.04?2.23). Further in vitro invasion assay using BEAS-2B and NIH3T3 cells stably transfected with overexpression constructs for full length wild-type AQP5 (AQP5) and its two mutants, N185D which blocks membrane trafficking and S156A which blocks phosphorylation on Ser156, showed that AQP5 induced cell invasions while both mutants did not. In BEAS-2B cells, the expression of AQP5 caused a spindle-like and fibroblastic morphologic change and losses of cell-cell contacts and cell polarity. Only cells with AQP5, not either of two mutants, exhibited a loss of epithelial cell markers and a gain of mesenchymal cell markers. In a human SH3-domains protein array, cellular extracts from BEAS-2B with AQP5 showed a robust binding activity to SH3-domains of the c-Src, Lyn, and Grap2 C-terminal. Furthermore, in immunoprecipitation assay, activated c-Src, phosphorylated on Tyr416, showed a stronger binding activity to cellular extracts from BEAS-2B with AQP5 compared with N185D or S156A mutant. Fluorescence in situ hybridization (FISH) analysis failed to show evidence of genomic amplification, suggesting AQP5 expression as a secondary event. Based on these clinical and molecular observations, we conclude that AQP5, through its phosphorylation on Ser156 and subsequent interaction with c-Src, plays an important role in NSCLC invasion and, therefore, may provide a unique opportunity for developing a novel therapeutic target as well as a prognostic marker in NSCLC.
Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae
Seryun Kim,Sook-Young Park,Kyoung Su Kim,Hee-Sool Rho,Myoung-Hwan Chi,Jaehyuk Choi,Jongsun Park,Sunghyung Kong,Jaejin Park,Jaeduk Goh,Yong-Hwan Lee
PLOS Genetics , 2009, DOI: 10.1371/journal.pgen.1000757
Abstract: The appropriate development of conidia and appressoria is critical in the disease cycle of many fungal pathogens, including Magnaporthe oryzae. A total of eight genes (MoHOX1 to MoHOX8) encoding putative homeobox transcription factors (TFs) were identified from the M. oryzae genome. Knockout mutants for each MoHOX gene were obtained via homology-dependent gene replacement. Two mutants, ΔMohox3 and ΔMohox5, exhibited no difference to wild-type in growth, conidiation, conidium size, conidial germination, appressorium formation, and pathogenicity. However, the ΔMohox1 showed a dramatic reduction in hyphal growth and increase in melanin pigmentation, compared to those in wild-type. ΔMohox4 and ΔMohox6 showed significantly reduced conidium size and hyphal growth, respectively. ΔMohox8 formed normal appressoria, but failed in pathogenicity, probably due to defects in the development of penetration peg and invasive growth. It is most notable that asexual reproduction was completely abolished in ΔMohox2, in which no conidia formed. ΔMohox2 was still pathogenic through hypha-driven appressoria in a manner similar to that of the wild-type. However, ΔMohox7 was unable to form appressoria either on conidial germ tubes, or at hyphal tips, being non-pathogenic. These factors indicate that M. oryzae is able to cause foliar disease via hyphal appressorium-mediated penetration, and MoHOX7 is mutually required to drive appressorium formation from hyphae and germ tubes. Transcriptional analyses suggest that the functioning of M. oryzae homeobox TFs is mediated through the regulation of gene expression and is affected by cAMP and Ca2+ signaling and/or MAPK pathways. The divergent roles of this gene set may help reveal how the genome and regulatory pathways evolved within the rice blast pathogen and close relatives.
Contamination Assessment of Abandoned Mines by Integrated Pollution Index in the Han River Watershed
Hosik Lee, Md. Imran Kabir, Phil Sang Kwon, Jong Myeong Kim, Jeong Gyu Kim, Seung Hun Hyun, Yeon Taek Rim, Myoung Soon Bae, Eul Ryul RyuMyung Sook JungChungcheongbuk-do
The Open Environmental Pollution & Toxicology Journal , 2009, DOI: 10.2174/1876397900901010027]
Abstract: Heavy metal contamination from abandoned mine areas is a major threat for the environment. The study objective was to categorize the most polluted mine areas among 44 mine sites at the four cities of Chungcheongbuk-do province in South Korea. Both water and soil samples were collected from the mine area. The pH of the water and soil ranged from 3.6 to 8.5 and from 4.1 to 9.1 respectively. A significant amount of arsenic (As), cadmium (Cd), copper (Cu), nickel (Ni), lead (Pb), zinc (Zn) and mercury (Hg) occurred in soil samples collected from the mine areas (0.2 to 42.4, 0.7 to 8.6, 10.7 to 430.2, 5.8 to 49.8, 2.1 to 122.8, 37.4 to 359.4 and 0.2 to 11.4 mg kg-1 respectively). The surrounding available waters also carried high contents of Cd, Cu, Pb and Zn, generally exceeding the fresh water acute and chronic criteria. Each mine site was ranked according to the Integrated Pollution Index (IPI). The normalized pollution index (PIn) for water and soil, and the Survey Index (SI) were used to determine IPI. The highest polluted mine site exhibited an IPI value of 0.6394. IPI was introduced to prioritize the research sites for further precise investigation.
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