oalib

Publish in OALib Journal

ISSN: 2333-9721

APC: Only $99

Submit

Search Results: 1 - 10 of 443 matches for " Mukesh Doble "
All listed articles are free for downloading (OA Articles)
Page 1 /443
Display every page Item
Modeling of D-Hydantoinase Production by Agrobacterium radiobacter in a Batch System
M. Annamalai,Mukesh Doble
Journal of Applied Sciences , 2007,
Abstract: Mathematical modeling of hydantoinase production system from microbial sources, which would help to understand the mechanism of the process, has not been attempted earlier. This paper tries to model five state variables (biomass, substrate, product (D-hydantoinase), Oxygen Uptake Rate (OUR) and carbon dioxide production rate (CPR)) for three carbon sources namely glucose, glycerol and maltose in the production of D-hydantoinase using Agrobacterium radiobacter as source. Several models were tested to fit the aerobic batch experimental data from a 3 L bioreactor. The best fitting model consisted of (a) biomass growth non-linearly dependent on substrate concentration, (b) product formation rate following exponential form of product inhibition and (c) OUR following positive regulation by substrate. D-hydantoinase production in maltose experiences minimal lag phase and stronger product inhibition when compared to glycerol. Maltose showed higher biomass yield (0.25) and specific D-hydantoinase production (27.44 U mg-1) compared to glycerol whose values are 0.18 and 21.97 U mg-1, respectively.
Pseudomonas aeruginosa BUP2—A Novel Strain Isolated from Malabari Goat Produces Type 2 Pyoverdine  [PDF]
Kizhakkepowathial Nair Unni, Prakasan Priji, Valerie A. Geoffroy, Mukesh Doble, Sailas Benjamin
Advances in Bioscience and Biotechnology (ABB) , 2014, DOI: 10.4236/abb.2014.511102
Abstract: This study focuses on the isolation and characterization of a novel strain of siderophore producing bacterium, i.e., Pseudomonas aeruginosa BUP2 (Pa BUP2) from the rumen of Malabari goat, coupled with qualitative and quantitative analyses of the siderophore produced by it. Pa BUP2a facultative anaerobe was tuned to be an aerobe by repeatedly growing in Benjamin flask. The new isolate was grown in a specially designed semi-synthetic medium, designated as BUP medium, and the yellowish-green pigment produced was identified as a typical siderophore by spectrophotometry, Chromazurol-S assay, thin layer chromatography and isolectric focusing (IEF). The characteristic orange fluorescence upon UV irradiation on chromatogram and absorption maximum at λ404 confirmed that the characteristic siderophore produced by Pa BUP2 was a typical pyoverdine (PVD). This PVD was further categorized under type 2 by comparing its profile on the IEF gel with that of the representative strains of each PVD types, viz., Pa O1, Pa
Production and downstream processing of (1→3)-β-D-glucan from mutant strain of Agrobacterium sp. ATCC 31750
Gayathiri T Kalyanasundaram, Mukesh Doble, Sathyanarayana N Gummadi
AMB Express , 2012, DOI: 10.1186/2191-0855-2-31
Abstract:
Docking studies on novel analogues of 8 methoxy fluoroquinolones against GyrA mutants of Mycobacterium tuberculosis
RS Anand, Sulochana Somasundaram, Mukesh Doble, CN Paramasivan
BMC Structural Biology , 2011, DOI: 10.1186/1472-6807-11-47
Abstract: Molecular docking and in vitro studies correlated well in demonstrating the enhanced activity of moxifloxacin, when compared to gatifloxacin, on ofloxacin sensitive and resistant strains comprising of clinical isolates of MDR-TB. The evolved lead structures targeting against mutant QRDR receptors were guanosine and cholesteryl esters of gatifloxacin and moxifloxacin. They showed consistently high binding affinity values of -10.3 and -10.1 kcal/mol respectively with the target receptors. Of these, the guanosine ester showed highest binding affinity score and its log P value lied within the Lipinski's range indicating that it could have better absorptivity when it is orally administered thereby having an enhanced activity against MTB.The docking results showed that the addition of the cholesteryl and guanosine esters to the 'DNA gyrase binding' region of gatifloxacin and moxifloxacin enhanced the binding affinity of these parent molecules with the mutant DNA gyrase receptors. Viewing the positive correlation for the docking and in vitro results with the parent compounds, these lead structures could be further evaluated for their in vitro and in vivo activity against MDR-TB.The resurgence of multi-drug resistant tuberculosis (MDR-TB) [1] and HIV associated intractable mycobacterial infection are of serious global concern [2]. To contain this situation, new anti-tuberculosis drugs and reduced regimen treatments are of immediate requirement. Development of novel antituberculosis compounds to combat MDR-TB is urgently needed. Unfortunately, except for rifabutin and rifapentine there are no new drugs available during the 40 years after the release of rifampicin. The discovery of new drugs involves several constraints that discourage many companies from investing in novel anti-TB drugs. The research is expensive, slow and difficult, and it requires specialized facilities for handling Mycobacterium tuberculosis (MTB). Due to this situation, it is a matter of urgency to devel
Antibiofilm Properties of Interfacially Active Lipase Immobilized Porous Polycaprolactam Prepared by LB Technique
Veluchamy Prabhawathi, Thulasinathan Boobalan, Ponnurengam Malliappan Sivakumar, Mukesh Doble
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096152
Abstract: Porous biomaterial is the preferred implant due to the interconnectivity of the pores. Chances of infection due to biofilm are also high in these biomaterials because of the presence of pores. Although biofilm in implants contributes to 80% of human infections [1], there are no commercially available natural therapeutics against it. In the current study, glutaraldehyde cross linked lipase was transferred onto a activated porous polycaprolactam surface using Langmuir-Blodgett deposition technique, and its thermostability, slimicidal, antibacterial, biocompatibility and surface properties were studied. There was a 20% increase in the activity of the covalently crosslinked lipase when compared to its free form. This immobilized surface was thermostable and retained activity and stability until 100°C. There was a 2 and 7 times reduction in carbohydrate and 9 and 5 times reduction in biofilm protein of Staphylococcus aureus and Escherichia coli respectively on lipase immobilized polycaprolactam (LIP) when compared to uncoated polycaprolactam (UP). The number of live bacterial colonies on LIP was four times less than on UP. Lipase acted on the cell wall of the bacteria leading to its death, which was confirmed from AFM, fluorescence microscopic images and amount of lactate dehydrogenase released. LIP allowed proliferation of more than 90% of 3T3 cells indicating that it was biocompatible. The fact that LIP exhibits antimicrobial property at the air-water interface to hydrophobic as well as hydrophilic bacteria along with lack of cytotoxicity makes it an ideal biomaterial for biofilm prevention in implants.
MDS and Trilateration Based Localization in Wireless Sensor Network  [PDF]
Shailaja Patil, Mukesh Zaveri
Wireless Sensor Network (WSN) , 2011, DOI: 10.4236/wsn.2011.36023
Abstract: Localization of sensor nodes is crucial in Wireless Sensor Network because of applications like surveillance, tracking, navigation etc. Various optimization techniques for localization have been proposed in literature by different researchers. In this paper, we propose a two phase hybrid approach for localization using Multidi- mensional Scaling and trilateration, namely, MDS with refinement using trilateration. Trilateration refines the estimated locations obtained by the MDS algorithm and hence acts as a post optimizer which improves the accuracy of the estimated positions of sensor nodes. Through extensive simulations, we have shown that the proposed algorithm is more robust to noise than previous approaches and provides higher accuracy for estimating the positions of sensor nodes.
Banach Λ-Frames for Operator Spaces  [PDF]
Mukesh Singh, Renu Chugh
Advances in Pure Mathematics (APM) , 2014, DOI: 10.4236/apm.2014.48048
Abstract:

The Banach frame for a Banach space X can reconstruct each vector in X by the pre-frame operator or the reconstruction operator. The Banach Λ-frame for operator spaces was introduced by Kaushik, Vashisht and Khattar [Reconstruction Property and Frames in Banach Spaces, Palestine Journal of Mathematics, 3(1), 2014, 11-26]. In this paper we give necessary and sufficient conditions for the existence of the Banach Λ-frames. A Paley-Wiener type stability theorem for Λ-Banach frames is discussed.

K-Means Graph Database Clustering and Matching for Fingerprint Recognition  [PDF]
Vaishali Pawar, Mukesh Zaveri
Intelligent Information Management (IIM) , 2015, DOI: 10.4236/iim.2015.74019
Abstract: The graph can contain huge amount of data. It is heavily used for pattern recognition and matching tasks like symbol recognition, information retrieval, data mining etc. In all these applications, the objects or underlying data are represented in the form of graph and graph based matching is performed. The conventional algorithms of graph matching have higher complexity. This is because the most of the applications have large number of sub graphs and the matching of these sub graphs becomes computationally expensive. In this paper, we propose a graph based novel algorithm for fingerprint recognition. In our work we perform graph based clustering which reduces the computational complexity heavily. In our algorithm, we exploit structural features of the fingerprint for K-means clustering of the database. The proposed algorithm is evaluated using realtime fingerprint database and the simulation results show that our algorithm outperforms the existing algorithm for the same task.
James Webb Space Telescope segment phasing using differential optical transfer functions
Johanan L. Codona,Nathan Doble
Physics , 2015, DOI: 10.1117/1.JATIS.1.2.029001
Abstract: Differential Optical Transfer Function (dOTF) is an image-based, non-iterative wavefront sensing method that uses two star images with a single small change in the pupil. We describe two possible methods for introducing the required pupil modification to the JWST, one using a small (
Pancreatic Cancer Biomarkers and Their Implication in Cancer Diagnosis and Epidemiology
Mukesh Verma
Cancers , 2010, DOI: 10.3390/cancers2041830
Abstract: Pancreatic cancer is the fourth most common cause of cancer-related mortality in the United States. Biomarkers are needed to detect this cancer early during the disease development and for screening populations to identify those who are at risk. In cancer, “biomarker” refers to a substance or process that is indicative of the presence of cancer in the body. A biomarker might be either a molecule secreted by a tumor or it can be a specific response of the body to the presence of cancer. Genetic, epigenetic, proteomic, glycomic, and imaging biomarkers can be used for cancer diagnosis, prognosis, and epidemiology. A number of potential biomarkers have been identified for pancreatic cancer. These markers can be assayed in non-invasively collected biofluids. These biomarkers need analytical and clinical validation so that they can be used for the purpose of screening and diagnosing pancreatic cancer and determining disease prognosis. In this article, the latest developments in pancreatic cancer biomarkers are discussed.
Page 1 /443
Display every page Item


Home
Copyright © 2008-2017 Open Access Library. All rights reserved.