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Search Results: 1 - 10 of 6982 matches for " Muhammad Ramzan "
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Immature rat seminal vesicles show histomorphological and ultrastructural alterations following treatment with kisspeptin-10
Faiqah Ramzan, Irfan Qureshi, Muhammad Ramzan, Muhammad Ramzan, Faiza Ramzan
Reproductive Biology and Endocrinology , 2012, DOI: 10.1186/1477-7827-10-18
Abstract: Prepubertal laboratory rats were injected kisspeptin-10 at three different dosage concentrations (10 pg, 1 ng and 1 microgram) for a period of continuous 12 days at the rate of two doses per day. Control rats were maintained in parallel. The day following the end of the experimental period, seminal vesicles were removed and processed for light and electron microscopic examination using the standard methods. DNA damage was estimated by DNA ladder assay and DNA fragmentation assay.The results demonstrated cellular degeneration. Epithelial cell height of seminal vesicles decreased significantly at all doses (P < 0.05). Marked decrease in epithelial folds was readily noticeable, while the lumen was dilated. Ultrastructural changes were characterized by dilatation of endoplasmic reticulum and Golgi complex, heterochromatization of nuclei, invagination of nuclear membranes and a decreased number of secretory granules. Percent DNA damage to the seminal vesicle was 19.54 +/- 1.98, 38.06 +/- 2.09 and 58.18 +/- 2.59 at 10 pg, 1 ng and 1 microgram doses respectively.The study reveals that continuous administration of kisspeptin does not lead to an early maturation but instead severe degeneration of sexually immature seminal vesicles.In 1999, Lee and colleagues discovered in the rat a novel G protein-coupled receptor, the GPR54. The GPR54 gene encodes a G protein-coupled receptor [1]. It was later shown to mediate the actions of a unique family of KiSS-1 derived endogenous ligands known as kisspeptins. The KiSS-1 gene encodes a 145-amino acid peptide that is cleaved into an amidated C-terminal 54 amino acid product, kisspeptin or metastin. Shorter fragments of kisspeptin-54, the kisspeptin-14, kisspeptin-13, and kisspeptin-10, also bind to GPR54. Kisspeptin-54 was originally identified as metastasis suppresser peptide from malignant melanoma cells that had been suppressed for metastatic potential by the introduction of human chromosome 6, hence named metastin [2-4]. In 2003 two
Characterization of purified β-glucosidase produced from Trichoderma viride through bio-processing of orange peel waste  [PDF]
Muhammad Irshad, Zahid Anwar, Muhammad Ramzan, Zahed Mahmood, Haq Nawaz
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.410124
Abstract:

In the present study, solid state fermentation was carried out using orange peel waste to produce β-glucosidase from Trichoderma viride. A locally isolated fungal strain T. viride was cultured in the solid state medium of orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity of 515 ± 12.4 U/mL was recorded after 4th day of incubation at pH 5.5 and 30℃. Indigenously produced β-glucosidase was subjected to the ammonium sulfate precipitation and Sephadex-G-100 gel filtration chromatography. In comparison to the crude extract β-glucosidase was 5.1-

Nutritional Status of Affluent School Children of Dera Ismail Khan: Is under Nutrition Common?
Muhammad Ramzan,Irshad Ali,Faiqah Ramzan,Faiza Ramzan
Pakistan Journal of Nutrition , 2010,
Abstract: The present study assesses the frequency of underweight in primary school children from families of high socioeconomic status in Dera Ismail Khan City, Pakistan. It included 322 children, 221 (68.63%) boys and 101 (31.37%) girls. The schools such as The Qurtruba, The City and The Educators were selected as they are the only institutions that cater wards of high socio economic group in Dera Ismail Khan. They were living in healthy environments, have excellent nutrition and easy access to health facilities. Thorough medical examination excluded those suffering from chronic health ailments. Height and weight of each child was taken. BMI was calculated according to the Quatelet’s Index and body mass status according to WHO S′ criteria. The BMI-for-age percentile was derived by putting BMI values on gender specific CDC S′ growth charts 2-20 years. Children falling below 5th percentile were declared as under weight and those >95th percentile as obese. Frequency of under weight in primary school children was found 5.59% (in sample): 4.03% for boys and 1.55% for girls. Boys were more than two times under weight than girls. This may be due to absence of gender discrimination; easy access of girls to kitchen and food stored in the house. It also reflects the changing attitude of these families about the status of women; improvement in the level of education, economics and knowledge about nutrition.
Exploitation of Concatenated Olive Plastome DNA Markers for Reliable Varietal Identification for On-Farm Genetic Resource Conservation  [PDF]
Muhammad Noman, Wajya Ajmal, Muhammad Ramzan Khan, Armghan Shahzad, Ghulam Muhammad Ali
American Journal of Plant Sciences (AJPS) , 2015, DOI: 10.4236/ajps.2015.619299
Abstract: Rapid and reliable identification of olive plants using DNA markers has been attempted in the past but the selection of polymorphic regions for discrimination at varietal level remained obscure. Recent sequencing of plastid genome of the olive flaunts high resolution Cp markers for olive DNA fingerprinting. Using this information, we designed a combination of chloroplast markers to amplify genes recruited in photosynthesis, ribosomal and NADH energy metabolism for varietal identification of olive plants. Concatenated DNA sequences of more than 100 unknown and 10 reference plants samples were analyzed using various bioinformatics and phylogenetic tools. Conserved blocks of nucleotide sequences were detected in multiple alignments. Phylogenetic reconstruction differentiated the unknown plants into various clusters with known varieties. Further narrowing down of the samples through UPGMA tree clearly separated the plants into Arbosana, Frantoio and Koroneiki as the major varieties. Multiple alignments of these clusters revealed important variety specific SNPs including G and T nucleotides at specific positions. Sequence identifying at intra cultivar level was more than 98.79% while it dropped to 97%, and even to 96% at inter varietal level. Furthermore, a neighbor net network analysis separated these three clusters, thus validating the results of UPGMA tree. Over all, out of 100 plants samples, 49 plants were identified that fall into 10 varieties including Arbosana, Carolea, Chetoui, Coratina, Domat, Frantoio, Gemlik, Koroneiki,Leccino and Moraiolo. The maximum number of known plants belongs to Frantoio and Gemlik (8 each). The least number of samples was identified from Carolea, Domat and Moraiolo with 2 samples each. However, 51 plants could not be identified, as plants were not clustered with any of reference control. Our results have implications in on-farm conservation of olive germplasm and provision of genuine material for multiplication of authentic varieties. This strategy can be extended to varietal identification of other plant species.
Sonographic Assessment of the Abdominal Wall Thickness in Primary School Children of Dera Ismail Khan, Pakistan
Muhammad Ramzan,Irshad Ali,Muhammad Yaqoob,Faiqah Ramzan
Pakistan Journal of Nutrition , 2011,
Abstract: The present study was carried out to assess the abdominal wall thickness/abdominal fat in school children (6-11 years) at Dera Ismail Khan Pakistan. Abdominal Ultrasound was the sole criteria for the assessment of abdominal wall thickness. It included 103 school children; 58 (56.31%) boys and 45 (43.68%) girls. 76 (73.79%) were obese and 27 (26.21%) were normal weight children. Thorough clinical examination excluded those with chronic health problems. Body weight status was determined according to Quatelet’s Index and CDC’s gender specific growth charts 2-20 years (2000). Those, having BMI-for-age-percentile 5th-<85th percentile were declared as normal weight and obese with >95th percentile. An ultasononic probe of 7.5MHZ was used to assess the abdominal fat (subcutaneous and preperitoneal) on two points: 2 cm above and 2 cm below umbilicus in the midline. Abdominal wall thickness/abdominal fat were found higher below the umbilicus than above the umbilicus in obese as well as in normal weight children. Maximum thickness of abdominal wall was found below the umbilicus in an obese girl (3.25 cm). Mean, for the abdominal thickness in normal weight children was calculated as 1cm. It is an important indicator for abdominal obesity in children.
Studies on the Rapid Clonal Propagation of Saccharum officinarum
Muhammad Ramzan Khan,Hamid Rashid
Pakistan Journal of Biological Sciences , 2003,
Abstract: A protocol for rapid in vitro propagation of Saccharum officinarum using meristem and axillary buds as explants was developed. Murashige and Skoog (MS) medium supplemented with 20 g l-1 sucrose, 2 g l-1 gelrite and various combination of kinetin (Kn) and gibberrellic acid (GA3) was used as a culture initiation medium. Thirty three percent of the apical buds and 20% of the axillary buds grew into shoots within 15 days on media containing 1 mg l-1 Kn and 0.1 mg l-1 GA3. Transferring the shoots after they were 5cm long on the liquid medium for 15 days led to the maximum multiplication of the shoots. Plantlets with profuse roots were obtained 10 days after the shoots were removed off and planted on the liquid medium containing 60 g l-1 of sucrose. More then 95% of the plantlets survived after one month when they were transplanted in the vermiculite.
A Note on Spherical Electromagnetic Wave Diffraction by a Perfectly Conducting Strip in a Homogeneous Bi-Isotropic Medium
Muhammad Ayub;Muhammad Ramzan;Amer Bilal Mann
PIER , 2008, DOI: 10.2528/PIER08071404
Abstract: We have studied the problem of diffraction of an electromagnetic spherical wave by a perfectly conducting finite strip in a homogeneous bi-isotropic medium and obtained some improved results. The problem was solved by using the Wiener-Hopf technique and Fourier transform. The scattered field in the far zone was determined by the method of steepest descent. The significance of the present analysis was that it recovered the results when a strip was widened into a half plane.
Iron Deficiency Anemia in School Children of Dera Ismail Khan, Pakistan
Muhammad Ramzan,Irshad Ali,Abdus Salam
Pakistan Journal of Nutrition , 2009,
Abstract: The prevalence of obesity has increased at an epidemic rate and obesity has become one of the most common health concerns in both developed and developing countries. Very few studies in Pakistan have noted a possible association between iron deficiency anemia and obesity. The objective of this study was to investigate the frequency of iron deficiency anemia in obese school children of Dera Ismail Khan (N.W.F.P), Pakistan. This study involved a total of 103 children (6-11 years) with 76 (73.79%) obese and 27 (26.21%) as healthy/normal weights, comprising 58 (56.31%) as the boys and 45 (43.69%) as the girls. Concentration of hemoglobin was used as the sole criteria for iron deficiency anemia. Children were considered iron deficient anemic if their hemoglobin concentration was found below 11.5g/dl. With the use of Centers of Disease Control and Prevention (CDC)'s age and gender specific Growth charts, obese were defined as having BMI of more than 95th percentile. In the present study none of the obese boys or girls was suffering from iron deficiency anemia. However, 58.82% healthy/normal weight boys were anemic, with maximum number at the age of 06 years. Similarly 70% healthy/normal weight girls were anemic, with maximum number at the age of 6 (100%), followed by 10 years (66.66%). Both are the ages of physical activity and growth spurt. Given the increasing number of obese children and known morbidities of iron deficiency anemia, screening for iron deficiency anemia may be included in children with elevated BMI for age percentiles.
Effects of Various Growth Regulators on Callus Formation and Regeneration in Brassica napus Cv. Oscar
Muhammad Ramzan Khan,Hamid Rashid,Azra Quraishi
Pakistan Journal of Biological Sciences , 2002,
Abstract: Hypocotyl explants from in vitro seedlings were cultured aseptically on Gamborg (B5) medium supplemented with various concentrations and combinations of dichlorophenoxyacetic acid, indoleacetic acid, naphthalene acetic acid and benzylaminopurine for callus formation and shoot regeneration. 2,4-D alone @ 2 mg/l gave the highest callus formation (96%). The results revealed that BAP @ 2 mg/l with IAA 0.5 mg/l was the most appropriate combination to produce the multiple shoots (14.5 shoots per culture). Efficient rooting was achieved on half-strength MS medium supplemented with 0.250mg/I IBA and 0.125mg/l IAA. In vitro raised plantlets were transferred to potted soil and finally to experimental fields to assess their acclimatizing potential.
Development of Aseptic Protocols in Olive (Olea europaea L.) cv. Pendollino
Muhammad Ramzan Khan,Hamid Rashid,Azra Quraishi
Asian Journal of Plant Sciences , 2002,
Abstract: An efficient protocol was developed for the establishment of aseptic conditions, as a pre-requisite for meristem tip culture of olive (Olea europa L.) CV. Pantaloon. Shoot tips 0.5-0.8mm in length were used as explants source from the field-grown olive plants. Before cultured on Olive Initial Medium (OIM) for shoot proliferation and callus induction, these shoots were disinfected with four different disinfection procedures. Sterile and growing cultures were obtained, only from explants sterilized with 2.5mm HgCl2 for 3 min followed by 50% Chlorox for 15min. Sterile explants responded to the growing media either by developing into leafy shoots or by producing callus. This paper outlines the aseptic protocols for obtaining the sterile explants as the starting material to initiate in vitro cultures in olive CV. Pantaloon.
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