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Search Results: 1 - 10 of 2905 matches for " Mozdarani Hossein "
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Biological Complexities in Radiation Carcinogenesis and Cancer Radiotherapy: Impact of New Biological Paradigms
Hossein Mozdarani
Genes , 2012, DOI: 10.3390/genes3010090
Abstract: Although radiation carcinogenesis has been shown both experimentally and epidemiologically, the use of ionizing radiation is also one of the major modalities in cancer treatment. Various known cellular and molecular events are involved in carcinogenesis. Apart from the known phenomena, there could be implications for carcinogenesis and cancer prevention due to other biological processes such as the bystander effect, the abscopal effect, intrinsic radiosensitivity and radioadaptation. Bystander effects have consequences for mutation initiated cancer paradigms of radiation carcinogenesis, which provide the mechanistic justification for low-dose risk estimates. The abscopal effect is potentially important for tumor control and is mediated through cytokines and/or the immune system (mainly cell-mediated immunity). It results from loss of growth and stimulatory and/or immunosuppressive factors from the tumor. Intrinsic radiosensitivity is a feature of some cancer prone chromosomal breakage syndromes such as ataxia telangectiasia. Radiosensitivity is manifested as higher chromosomal aberrations and DNA repair impairment is now known as a good biomarker for breast cancer screening and prediction of prognosis. However, it is not yet known whether this effect is good or bad for those receiving radiation or radiomimetic agents for treatment. Radiation hormesis is another major concern for carcinogenesis. This process which protects cells from higher doses of radiation or radio mimic chemicals, may lead to the escape of cells from mitotic death or apoptosis and put cells with a lower amount of damage into the process of cancer induction. Therefore, any of these biological phenomena could have impact on another process giving rise to genome instability of cells which are not in the field of radiation but still receiving a lower amount of radiation. For prevention of radiation induced carcinogenesis or risk assessment as well as for successful radiation therapy, all these phenomena should be taken into account.
Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
MOZDARANI,HOSSEIN; MORADI,SHABNAM Z;
Biological Research , 2007, DOI: 10.4067/S0716-97602007000400004
Abstract: this study was desμgned to investμgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. to this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from nmri mice 3 days after mating. retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. after appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. after 48 hours, the embryos were fixed and studied for their chromosome abnormalities using tarkowsky's drying technique. results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). this result mμght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mμght produce chromosomal alterations leading to cell death
Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
HOSSEIN MOZDARANI,SHABNAM Z MORADI
Biological Research , 2007,
Abstract: This study was desμgned to investμgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. After appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. After 48 hours, the embryos were fixed and studied for their chromosome abnormalities using Tarkowsky's drying technique. Results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. Furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). This result mμght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mμght produce chromosomal alterations leading to cell death
Impact of pericentric inversion of Chromosome 9 [inv (9) (p11q12)] on infertility
Mozdarani Hossein,Meybodi Anahita,Karimi Hamideh
Indian Journal of Human Genetics , 2007,
Abstract: Background : One of the frequent occurrences in chromosome rearrangements is pericentric inversion of the Chromosome 9; inv (9) (p11q12), which is consider to be the variant of normal karyotype. Although it seems not to correlate with abnormal phenotypes, there have been many controversial reports indicating that it may lead to abnormal clinical conditions such as infertility. The incidence is found to be about 1.98% in the general population. Materials and Methods : We investigated the karyotypes of 300 infertile couples (600 individuals) being referred to our infertility clinic using standard GTG banding for karyotype preparation. Results : The chromosomal analysis revealed a total of 15 (2.5%) inversions, among these, 14 male patients were inversion 9 carriers (4.69%) while one female patient was affected (0.33%). The incidence of inversion 9 in male patients is significantly higher than that of normal population and even than that of female patients (P< 0.05). Conclusions : This result suggests that inversion 9 may often cause infertility in men due to spermatogenic disturbances, which are arisen by the loops or acentric fragments formed in meiosis.
Lucifer Yellow uptake by CHO cells exposed to magnetic and electric pulses
Leila Towhidi, , Seyed Mohammad P Firoozabadi, Hossein Mozdarani, Damijan Miklavcic
Radiology and Oncology , 2012, DOI: 10.2478/v10019-012-0014-2
Abstract: Background. The cell membrane acts as a barrier that hinders free entrance of most hydrophilic molecules into the cell. Due to numerous applications in medicine, biology and biotechnology, the introduction of impermeant molecules into biological cells has drawn considerable attention in the past years. One of the most famous methods in this field is electroporation, in which electric pulses with high intensity and short duration are applied to the cells. The aim of our study was to investigate the effect of time-varying magnetic field with different parameters on transmembrane molecular transport. Materials and methods. ‘Moreover, a comparison was made between the uptake results due to magnetic pulse exposure and electroporation mediated uptake.’ at the end of Background part. The Chinese hamster ovary (CHO) cells were exposed to magnetic pulses of 2.2 T peak strength and 250 μs duration delivered by Magstim stimulator and double 70 mm coil. Three different frequencies of 0.25, 1 and 10 Hz pulses with 112, 56 and 28 number of pulses were applied (altogether nine experimental groups) and Lucifer Yellow uptake was measured in each group. Moreover, maximum uptake of Lucifer Yellow obtained by magnetic pulses was compared to the measured uptake due to electroporation with typical parameters of 8 pulses of 100 μs, repetition frequency of 1 Hz and electric field intensities of 200 to 600 V/cm. Results and conclusions. Our results show that time-varying magnetic field exposure increases transmembrane molecular transport and this uptake is greater for lower frequencies and larger number of pulses. Besides, the comparison shows that electroporation is more effective than pulsed magnetic field, but the observed uptake enhancement due to magnetic exposure is still considerable.
Evaluation of Cytogenetic Changes and FLT3 mutations in Patients with Acute Promyelocytic Leukemia
Marjan Yaghmaie,Hossein Mozdarani,Kamran Alimoghaddam,Ardeshir Ghavamzadeh
International Journal of Hematology-Oncology and Stem Cell Research , 2010,
Abstract: "nIntroduction: The secondary genetic changes other than the PML-RARA fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia (AML). However, the prognostic significance of FLT3 mutations in acute promyelocytic leukemia (APL) is not firmly established. "nPatients & Methods: FLT3 ITD screening by fragment length analysis and FLT3 D835 mutation by melting curve analysis in 23 APL samples was screened in this study. "nResults: About13% of the patients had FLT3 internal tandem duplications (ITDs), and 26% had D835 point mutation. FLT3 ITD mutation was associated with higher white blood cell (WBC) count at presentation and poor prognosis. "nConclusions: As the PML-RARA is not sufficient to develop APL, we assume FLT3 mutations and additional chromosomal alterations in this APL series may cooperate with PML-RARA in APL development. "n "n "n
A cytogenetic study of couples with recurrent spontaneous abortions and infertile patients with recurrent IVF/ICSI failure
Mozdarani Hossein,Meybodi Anahita,Zari-Moradi Shabnam
Indian Journal of Human Genetics , 2008,
Abstract: Purpose: This study was conducted to determine the frequency and contribution of chromosomal abnormalities in miscarriages and in couples with recurrent in vitro fertilization/intra cytoplasmic sperm injection (IVF/ICSI) failure. Materials and Methods: A total of 221 individuals; 79 with three or more recurrent spontaneous abortions and 142 with at least three IVF/ICSI failures. Chromosomal analysis from peripheral blood lymphocytes was performed according to standard cytogenetic methods using G-banding technique. Results: Abnormal karyotype was found in 21 (9.50%) individuals. Of these 21 subjects, 4 (19.04%) exhibited sex chromosomal abnormalities and 17 (80.96%) had autosomal abnormalities. Male partners had significantly higher chromosomal abnormalities (5.88%) than of females (3.61%). These abnormalities were also higher in patients with recurrent spontaneous abortions than with IVF/ICSI failure (P < 0.05). Conclusions: These data may be indicative that chromosomal abnormalities are involved more in spontaneous abortions than in recurrent IVF/ICSI failure. Cytogenetic analysis could be valuable for these couples when clinical data fail to clarify the cause.
A Co-culture System for Expansion of Nonenriched Cord Blood Stem/Progenitor Cells
Masoud Soleimani,Hossein Mozdarani,AliAkbar Pourfathollah,Yousef Mortazavi
Biotechnology , 2005,
Abstract: Many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. The proliferation and expansion of Human Hematopoietic Stem Cells (HSC) in ex vivo culture was examined with the goal of generating a suitable protocol for expanding HSC for patient transplantation. Using primary fetal liver cells, we established a serum-free culture system to expand human primitive stem/progenitors cells. Non enriched cord blood CD34+ cells were cultured on a monolayer of mouse primary fetal liver cells in the presence of trombopoietin, Flt3/Flk2 ligand and/or stem cell factor, IL-6 and IL-3 under serum-free conditions. After 1 or 2 weeks of culture, cells were examined for clonogenic progenitors and percentage of CD34+, CD38 cells. In the presence of trombopoietin, Flt3/Flk2 ligand and stem cell factor, fetal liver cells supported more than a 10- to 20-fold expansion of CD34+ cells. In addition, CFU-C assay were expanded more than 5 and 10 fold after 1 and 2 weeks of culture, respectively. These results strongly suggest that fetal liver cells may be a suitable feeder layer for expansion of hematopoietic progenitors from umbilical cord blood in vitro.
Chromosome Abnormalities and Viability of Vitrified Eight-Cell Mouse Embryos at Presence of Two Different Cryoprotectants at Different Storage Durations
Shabnam Zarei Moradi,Anahita Mohseni Meybodi,Hamid Gourabi,Hossein Mozdarani
Cell Journal , 2013,
Abstract: Objective: Experiments were conducted to find the differences between post-thaw viability and chromosome aberrations in eight-cell mouse embryos at presence of dimethyl sulfoxide (DMSO) and 1, 2-propanediol (PROH) as croprotectants in different storage durations.Materials and Methods: In this case-control study, a total number of 720 mouse embryos from about 250 NMRI mice were vitrified with 30% PROH or DMSO; each diluted with a solution containing 30% ficol plus 0.5 M sucrose. Embryos were exposed to the solutions for 0.5 minute at 25 C followed by cooling in liquid nitrogen, then after appropriate storage duration, they were rapidly warmed. Besides, there were 100 mouse embryos for each cryoprotectant group (totally 200 embryos) as control. Embryo survival was assessed by in vitro development, and chromosome abnormalities were analyzed by Giemsa staining.Results: The proportion of mitotic abnormalities in PROH/DMSO vitrified embryos was significantly higher than unfrozen control group. This was confirmed also by a reduced viability of the embryos as judged by a culture at the blastocyst stage (p<0.05 in all test groups).Conclusion: It can be deduced that long term cryopreservation may result in chromosomal abnormalities and/or low viability.
Increase of CXCR4 Expression on Expanded Non-enriched Cord Blood CD34+ Cells Using MSCs
Masoud Soleimani,Hossein Mozdarani,Ali Akbar Pourfathollah,Yousef Mortazavi
Cell Journal , 2005,
Abstract: Introduction: A number of potential cell adhesion molecules, which mediate essential cell-to-cell or cell-to-matrix interactions, are expressed on the surface of CD34+ hematopoietic progenitor cells (HPCs), including integrins, CD44, and CXCR4. These molecules are essential for homing process. In this study, we compared the changes of expression of CD44 and CXCR4 on the CD34+ hematopoietic progenitor cells expanded on MSCs in the presence of cytokines. Material and Methods: Cord blood CD34+ cells were expanded using human bone marrow mesenchymal stem cells and cytokines (TPO, SCF, FLt-3, IL-6, and IL-3), and then expression of CD44 and CXCR4 on CD34+ cells were evaluated by flow cytometric analysis. Results: After 2 weeks of serum free culture of CD34+ cells in the presence of cytokines, the expression of CXCR4 on CD34+ cells was decreased 3.4 fold (p<0.05). In contrast, the expression of CXCR4 on CD34+ cells expanded on hMSCs was increased (p<0.05). The expression of CD44 on expanded CD34+ cells in both methods did not differ significantly. Conclusions: Our results indicated that co-culture of cord blood stem cells on hMSCs significantly increased CXCR4 expression on cord blood CD34+ cells.
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