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Search Results: 1 - 10 of 737 matches for " Morteza Pourfarzam "
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EFFECT OF ADDING THE INTERNAL STANDARD TO BLOOD SAMPLES, PRIOR TO THE PREPARATION OF BLOOD SPOTS FOR ACYLCARNITINE ANALYSIS
Osorio,José Henry; Pourfarzam,Morteza;
Biosalud , 2010,
Abstract: background: some general factors can influence when determining acylcarnitines through tandem mass spectrometry. objective: to study the effect of adding the internal standard to blood samples before the preparation of filter paper cards compared with the addition of internal standard after having the filter paper cards prepared for determining acylcarnitines in blood for tandem mass spectrometry. methodology: two groups of blood samples were prepared: group one without adding internal standard before the preparation of filter paper cards, and group two adding internal standard prior to the preparation of filter paper cards. subsequently the acylcarnitines profile was measured using tandem mass spectrometry. results: the recovery of acylcarnitines during extraction for the samples which were not added the internal standard before the preparation of the filter paper cards was 48% while for the samples that were added the internal standard the recovery percentage was 96% which shows a significant difference between the two groups. conclusion: the ideal extraction process showing the highest acylcarnitines recovery level happens by adding the internal standard to the sample prior to the preparation of filter paper cards. however, for diagnostic purposes, filter paper cards containing samples without internal standard are normally received for the acylcarnitines analysis and, therefore, the method used to add the internal standard after preparing the samples on filter paper without internal standard is appropriate for determining acylcarnitines in blood. abbreviations: esi-ms/ms, electrospray and tandem mass spectrometry.
MEMBRANE ACYL-CoA TRAFFIC AND REMAINING LEVELS IN RED BLOOD CELLS, PLASMA AND SERUM ANALYSED BY TANDEM MASS SPECTROMETRY
Osorio,José Henry; Pourfarzam,Morteza;
Biosalud , 2009,
Abstract: there has been a permanent question about the ideal fluid for carnitine and acylcarnitine analysis by tandem mass spectrometry. the present study evaluates the percentage of carnitine and acylcarnitines in red blood cells and the relationship with the carnitine and acylcarnitines content in whole blood, plasma, and serum. human blood samples were centrifuged, plasma or serum extracted, and blood cells were washed with different isotonic solutions. the final pellet was resuspended in pbs for card preparation and tandem mass spectrometry analysis. it was found that carnitine, short-chain, medium-chain and longchain acylcarnitines remain in red blood cells at average percentages of 43.4; 48; 49; and 70% respectively. a significant difference was found between carnitine and acylcarnitine levels in whole blood compare to its levels in plasma or serum (p<0.05). as carnitine and acylcarnitines remained associated with the blood cells, it seems therefore that plasma (or serum) is not the ideal material for the analysis of carnitine and acylcarnitines.
Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions
Osorio,José Henry; Pourfarzam,Morteza;
Colombia Médica , 2010,
Abstract: objective: to evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution. materials and methods: human blood samples were centrifuged and the blood cells were washed with different saline solutions. the final pellet was resuspended in pbs for card preparation and tandem mass spectrometry analysis. results: it was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05). conclusion: because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.
Levels of carnitine and acylcarnitines in reconstituted red blood cell samples washed with different concentrations of saline solutions
José Henry Osorio,Morteza Pourfarzam
Colombia Médica , 2010,
Abstract: Objective: To evaluate the percentage of carnitine and acylcarnitines remaining in red blood cells after washing them with different concentrations of saline solution.Materials and methods: Human blood samples were centrifuged and the blood cells were washed with different saline solutions. The final pellet was resuspended in PBS for card preparation and tandem mass spectrometry analysis.Results: It was found that carnitine, as well as short-chain, medium-chain, and long-chain acylcarnitines remain in red blood cells at average percentages of 19.3; 34; 34; and 32%, respectively. Significant differences were found for carnitine and acylcarnitine levels in blood washed with an isotonic solution compared to their levels using several hypotonic solutions (p<0.05).Conclusion: Because carnitine and acylcarnitines remained associated with the blood cells, we recommend using whole blood to measure these metabolites.
Hidrólisis de acilcarnitinas durante el análisis en sangre y plasma por espectrometría de masas en tandem
Osório,José Henry; Pourfarzam,Morteza;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: the measurement of acylcarnitines in blood is an important tool for diagnosis of some inherited metabolic diseases and secondary metabolic deficiencies. under the acidic conditions and the high temperature used for the derivatisation process, it is feasible that some degree of hydrolysis of acylcarnitines to free carnitine may occur and therefore potentially interfere with free carnitine measurements. the objective of the present study was to investigate the hydrolysis of acylcarnitines (short-chain-, medium-chain, and long chain acylcarnitines) during derivatisation process and to analyse its effect on free carnitine measurement. the average percentage of hydrolysis was 27% for short-chain acylcarnitines, 17% for medium-chain acylcarnitines, and 5% for long-chain acylcarnitines. these results can increase the free carnitine levels in the analysed samples.
Hidrólisis de acilcarnitinas durante el análisis en sangre y plasma por espectrometría de masas en tandem Hydrolysis of acylcarnitines during measurement in blood and plasma by tandem mass spectrometry
José Henry Osório,Morteza Pourfarzam
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: La determinación de acilcarnitinas en sangre es una herramienta importante para el diagnóstico de algunas enfermedades hereditarias, así como deficiencias metabólicas secundarias. Bajo las condiciones acídicas y la alta temperatura utilizadas durante el proceso de derivatización, es posible que pueda ocurrir algún grado de hidrólisis de acilcarnitinas, lo cual puede potencialmente interferir con las determinaciones de la carnitina libre. El objetivo del presente estudio fue investigar la hidrólisis de las acilcarnitinas (de cadena corta, media y larga) durante el proceso de derivatización y analizar su efecto sobre la determinación de carnitina libre. El porcentaje de hidrólisis fue de 27% para acilcarnitinas de cadena corta, 17% para acilcarnitinas de cadena media y 5% para acilcarnitinas de cadena larga. Estos resultados pueden ocasionar un incremento en los niveles de carnitina libre de las muestras analizadas. The measurement of acylcarnitines in blood is an important tool for diagnosis of some inherited metabolic diseases and secondary metabolic deficiencies. Under the acidic conditions and the high temperature used for the derivatisation process, it is feasible that some degree of hydrolysis of acylcarnitines to free carnitine may occur and therefore potentially interfere with free carnitine measurements. The objective of the present study was to investigate the hydrolysis of acylcarnitines (short-chain-, medium-chain, and long chain acylcarnitines) during derivatisation process and to analyse its effect on free carnitine measurement. The average percentage of hydrolysis was 27% for short-chain acylcarnitines, 17% for medium-chain acylcarnitines, and 5% for long-chain acylcarnitines. These results can increase the free carnitine levels in the analysed samples.
Association between Plasma Myeloperoxidase and Free 3-Nitrotyrosine Levels in Patients with Coronary Artery Disease  [PDF]
Morteza Pourfarzam, Ahmad Movahedian, Nizal Sarrafzadegan, Gholam Basati, Saed Ziaaldin Samsamshariat
International Journal of Clinical Medicine (IJCM) , 2013, DOI: 10.4236/ijcm.2013.43028
Abstract: Objective: Myeloperoxidase (MPO) is an inflammatory enzyme that is mainly released by activated neutrophils and monocytes. 3-nitrotyrosine (NT) is a stable inflammatory end product of MPO that is produced through nitrosylation of free and protein-bound tyrosines. Determination of the exact levels of free NT is technically a challenging matter. Also, there is limited information about the relationship between MPO and free NT levels and elevation of them in the plasma of patients with coronary artery disease (CAD). Therefore, we sought to determine the exact level of plasma free NT with a simple and exquisite technique in CAD patients. Methods: This study included 50 stable angina, 50 unstable angina patients, and 50 control subjects. Plasma MPO concentration was measured with an immunoassay method. Plasma free NT level was determined by a modified HPLC-fluorescence method. Lipid profile, high sensitivity C-reactive protein (hsCRP) and other clinical risk factors of patients were also assigned. Results: Plasma level of free NT was efficiently measured by the HPLC-fluorescence method. Plasma levels of MPO and NT were significantly higher in patients with stable and unstable CAD than in control subjects (P < 0.001). There was a significant correlation between the two substances in CAD patients (P < 0.001). Conclusions: We determined plasma free NT levels with a sensitive HPLC-fluorescence method with some modifications in a clinical scale. Plasma levels of MPO and NT were profoundly elevated in CAD patients. The significant relationships of the two substances and elevation of them may have useful clinical implication in patients with stable and
Reduced plasma adiponectin levels relative to oxidized low density lipoprotein and nitric oxide in coronary artery disease patients
Basati, Gholam;Pourfarzam, Morteza;Movahedian, Ahmad;Samsamshariat, Saed Ziaaldin;Sarrafzadegan, Nizal;
Clinics , 2011, DOI: 10.1590/S1807-59322011000700002
Abstract: introduction: adiponectin is a circulating hormone that is produced exclusively by adipocytes and has antiinflammatory and anti-atherogenic properties. the hypothesis that there are differences in adiponectin levels between stable and unstable coronary-artery disease patients remains controversial. furthermore, the potential relationships between the plasma adiponectin level and the inflammatory and non-inflammatory markers (oxidized low density lipoprotein and nitric oxide) in patients with stable and unstable coronary-artery disease relative to normal subjects have not been assessed. objectives: to assess whether plasma adiponectin levels differ among patients with stable and unstable coronary-artery disease and among control subjects, and to correlate plasma adiponectin level with inflammatory and clinical risk factors (such as oxidized-ldl and nitric oxide) in these patients. methods: this study included 50 control subjects, 50 stable angina patients and 50 unstable angina patients with angiographically documented coronary-artery disease. plasma adiponectin and oxidized-ldl levels were determined using an enzyme immunoassay. plasma nitric oxide, high sensitivity c-reactive protein and lipid profile levels were also measured. results: plasma adiponectin levels were lower in the unstable angina patients (4.9 ± 1.30 μg/ml) than in the stable angina patients (6.34 ± 1.0 μg/ml) or in the controls (9.25 ± 1.8 μg/ml); these levels were also significantly lower in stable angina patients versus controls (p<0.001). plasma adiponectin levels were negatively correlated with oxidized-ldl, high sensitivity c-reactive protein, lipid profile and other clinical risk factors but positively correlated with nitric oxide. conclusion: plasma adiponectin levels were found to be lower in both stable and unstable angina patients relative to control subjects, and the correlation between plasma adiponectin and cardiovascular markers is weakened in these patients.
Association of the Total Cholesterol Content of Erythrocyte Membranes with the Severity of Disease in Stable Coronary Artery Disease
Gholamreza Namazi,Morteza Pourfarzam,Sabieh Jamshidi Rad,Ahmad Movahedian Attar,Nizal Sarrafzadegan,Masoumeh Sadeghi,Parastoo Asa
Cholesterol , 2014, DOI: 10.1155/2014/821686
Abstract: Increasing evidence suggests that erythrocytes may participate in atherogenesis. We sought to investigate whether the total cholesterol content of erythrocyte membranes (CEM) is significantly different in patients with stable coronary artery disease (CAD) compared to patients with nonsignificant coronary stenosis and determine the correlation between CEM and the severity of coronary stenosis. Methods. The population included 144 patients, undergoing clinically indicated coronary angiography. The severity of coronary stenosis was scored after coronary angiography and patients were divided into two groups; the -stenosis group (CAD patients, ) had a significant stenosis indicated by coronary angiography and the second group, -stenosis (), had nonsignificant coronary stenosis. Lipid parameters were determined by routine laboratory methods. CEM was measured using an enzymatic assay, and protein content was assessed by the modified Lowry method. Results. The mean of CEM levels was higher () in stable CAD patients (137.2?μg/mg of membrane protein) compared with -stenosis patients (110.0?μg/mg of membrane protein). The coronary artery scores were correlated positively with CEM levels (, ). Conclusion. CEM levels are positively associated with the severity of CAD, meaning that CEM might contribute to the development of CAD. 1. Introduction Coronary artery disease (CAD) is closely associated with advanced atherosclerosis, which reflects several deteriorative phenomena that gradually result in narrowing of coronary arteries, terminating in thrombosis and myocardial infarction. CAD is one of the major causes of mortality and morbidity in both developed and developing countries and is believed to have a multifactorial etiology, composed of numerous biological, environmental, behavioral, and sociocultural factors [1–3]. In addition to traditional risk factors, erythrocyte membrane has been regarded as one of the most important contributors to the initiation and progression of atherosclerosis [4–11]. Although apoptotic macrophages are an important source of cholesterol within atherosclerotic plaques, it is unlikely that all of the cholesterol contained in plaques derives from foam cells alone. Most of the cholesterol in foam cell is esterified [12], whereas the atherosclerotic lipid core has a remarkably high content of free cholesterol [13]. The most compelling evidence was reported by Arbustini et al. [14], who identified an erythrocyte membrane protein, glycophorin A, in pulmonary plaques from patients with thromboembolic disease. These findings were repeated in
Serum Paraoxonase 1 Activity Is Associated with Fatty Acid Composition of High Density Lipoprotein
Maryam Boshtam,Amirnader Emami Razavi,Morteza Pourfarzam,Mohsen Ani,Gholam Ali Naderi,Gholam Basati,Marjan Mansourian,Narges Jafari Dinani,Seddigheh Asgary,Soheila Abdi
Disease Markers , 2013, DOI: 10.1155/2013/612035
Abstract: Introduction. Cardioprotective effect of high density lipoprotein (HDL) is, in part, dependent on its related enzyme, paraoxonase 1 (PON1). Fatty acid composition of HDL could affect its size and structure. On the other hand, PON1 activity is directly related to the structure of HDL. This study was designed to investigate the association between serum PON1 activity and fatty acid composition of HDL in healthy men. Methods. One hundred and forty healthy men participated in this research. HDL was separated by sequential ultracentrifugation, and its fatty acid composition was analyzed by gas chromatography. PON1 activity was measured spectrophotometrically using paraxon as substrate. Results. Serum PON1 activity was directly correlated with the amount of stearic acid and dihomo-gamma-linolenic acid (DGLA). PON1/HDL-C was directly correlated with the amount of miristic acid, stearic acid, and DGLA and was inversely correlated with total amount of ω6 fatty acids of HDL. Conclusion. The fatty acid composition of HDL could affect the activity of its associated enzyme, PON1. As dietary fats are the major determinants of serum lipids and lipoprotein composition, consuming some special dietary fatty acids may improve the activity of PON1 and thereby have beneficial effects on health. 1. Introduction Serum paraoxonase 1 (PON1) is a 45?kDa glycoprotein which can catalyze the hydrolysis of various organophosphates and nerve agents [1, 2] and also metabolize some drugs and prodrugs by its lactonase activity [3]. This enzyme which is located on high density lipoprotein (HDL) particles protects low density lipoprotein (LDL) phospholipids against oxidation [4]. Decreased PON1 activity has been addressed in several diseases such as coronary artery diseases (CAD) [5], type I diabetes [6], obesity [7], and renal failure [8]. It is evident that PON1 activity is influenced by a variety of agents like environmental, pharmacological, and lifestyle factors as well as age and sex [2, 5, 9–11]. Dietary fats have been suggested as an important relevant factor [12, 13]. Studies have presented that dietary fatty acids may affect PON1 activity [14]. Polyenoic fatty acids have shown considerable inhibitory effect on PON1 activity [15], while monoenoic acids (especially oleic acid) protect PON1 from oxidative inactivation [16]. It has been also indicated that replacement of dietary saturated fats with trans fats in healthy men and women leads to a small reduction in the serum PON1 activity [17]. Serum PON1 is almost exclusively found in association with HDL particles. The lipid
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