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Search Results: 1 - 10 of 6370 matches for " Moon-Jung Goo "
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Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice
Moon-Jung Goo,Jin-Kyu Park,Il-Hwa Hong,Ah-Young Kim,Eun-Mi Lee,Eun-Joo Lee,Meeyul Hwang,Kyu-Shik Jeong
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms140611084
Abstract: Recently, senescence marker protein-30 (SMP30) knockout (KO) mice have been reported to be susceptible to apoptosis, however, the role of SMP30 has not been characterized in the small intestine. The aim of the present study is to investigate the role of SMP30 in the process of spontaneous and γ-radiation-induced apoptosis in mouse small intestine. Eight-week-old male wild-type (WT) mice and SMP30 KO mice were examined after exposure to 0, 1, 3, 5, and 9 Gy of γ-radiation. Apoptosis in the crypts of the small intestine increased in the 0 to 5 Gy radiated SMP30 KO and WT mice. Radiation-induced apoptosis and the BAX/Bcl-2 ratio in the SMP30 KO mice were significantly increased in comparison to each identically treated group of WT mice ( p < 0.05). The levels of spontaneous apoptosis in both WT and KO mice were similar ( p > 0.05), indicating that increased apoptosis of crypt cells of SMP30 KO by irradiation can be associated with SMP30 depletion. These results suggested that SMP30 might be involved in overriding the apoptotic homeostatic mechanism in response to DNA damage.
Smad3 Deficiency Ameliorates Hepatic Fibrogenesis through the Expression of Senescence Marker Protein-30, an Antioxidant-Related Protein
Da-Hee Jeong,Meeyul Hwang,Jin-Kyu Park,Moon-Jung Goo,Il-Hwa Hong,Mi-Ran Ki,Akihito Ishigami,Ah-Young Kim,Eun-Mi Lee,Eun-Joo Lee,Kyu-Shik Jeong
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms141223700
Abstract: Smad3 is a key mediator of the transforming growth factor (TGF)-β1 signaling pathway that plays central role in inflammation and fibrosis. In present study, we evaluated the effect of Smad3 deficiency in Smad3?/? mice with carbon tetrachloride (CCl 4)-induced liver fibrosis. The animals were received CCl 4 or olive oil three times a week for 4 weeks. Histopathological analyses were performed to evaluate the fibrosis development in the mice. Alteration of protein expression controlled by Smad3 was examined using a proteomic analysis. CCl 4-induced liver fibrosis was rarely detected in Smad3?/? mice compared to Smad3+/+. Proteomic analysis revealed that proteins related to antioxidant activities such as senescence marker protein-30 ( SMP30), selenium-binding proteins (SP56) and glutathione S-transferases (GSTs) were up-regulated in Smad3?/? mice. Western blot analysis confirmed that SMP30 protein expression was increased in Smad3?/? mice. And SMP30 levels were decreased in CCl 4-treated Smad3 + / + and Smad3?/? mice. These results indicate that Smad3 deficiency influences the proteins level related to antioxidant activities during early liver fibrosis. Thus, we suggest that Smad3 deteriorate hepatic injury by inhibitor of antioxidant proteins as well as mediator of TGF-β1 signaling.
Identification of Heat Shock Protein 60 as a Regulator of Neutral Sphingomyelinase 2 and Its Role in Dopamine Uptake
Kyong-Hoon Ahn, Seok-Kyun Kim, Jong-Min Choi, Sung-Yun Jung, Jong-Hoon Won, Moon-Jung Back, Zhicheng Fu, Ji-Min Jang, Hae-Chan Ha, Dae-Kyong Kim
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067216
Abstract: Activation of sphingomyelinase (SMase) by extracellular stimuli is the major pathway for cellular production of ceramide, a bioactive lipid mediator acting through sphingomyelin (SM) hydrolysis. Previously, we reported the existence of six forms of neutral pH–optimum and Mg2+-dependent SMase (N-SMase) in the membrane fractions of bovine brain. Here, we focus on N-SMase ε from salt-extracted membranes. After extensive purification by 12,780-fold with a yield of 1.3%, this enzyme was eventually characterized as N-SMase2. The major single band of 60-kDa molecular mass in the active fractions of the final purification step was identified as heat shock protein 60 (Hsp60) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis. Proximity ligation assay and immunoprecipitation study showed that Hsp60 interacted with N-SMase2, prompting us to examine the effect of Hsp60 on N-SMase2 and ceramide production. Interestingly, Hsp60 siRNA treatment significantly increased the protein level of N-SMase2 in N-SMase2-overexpressed HEK293 cells. Furthermore, transfection of Hsp60 siRNA into PC12 cells effectively increased both N-SMase activity and ceramide production and increased dopamine re-uptake with paralleled increase. Taken together, these results show that Hsp60 may serve as a negative regulator in N-SMase2-induced dopamine re-uptake by decreasing the protein level of N-SMase2.
The assessment of efficacy of porcine reproductive respiratory syndrome virus inactivated vaccine based on the viral quantity and inactivation methods
Hyunil Kim, Hye Kim, Jung Jung, Yoo Choi, Jiho Kim, Chang Um, Su Hyun, Sungho Shin, Byeongchun Lee, Goo Jang, Bo Kang, Hyoung Moon, Dae Song
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-323
Abstract: In all groups, the sample to positive (S/P) ratio of IDEXX ELISA and the virus neutralization (VN) titer remained negative until challenge. While viremia did not reduce in the vaccinated groups, the IDEXX-ELISA-specific immunoglobulin G increased more rapidly and to significantly greater levels 7 days after the challenge in all the vaccinated groups compared to the non-vaccinated groups (p < 0.05). VN titer was significantly different in the 106 PFU/mL PRRSV vaccine-inoculated and binary ethylenimine (BEI)-inactivated groups 22 days after challenge (p < 0.05). Consequently, the inactivated vaccines tested in this study provided weak memory responses with sequential challenge without any obvious active immune responses in the vaccinated pigs.The inactivated vaccine failed to show the humoral immunity, but it showed different immune response after the challenge compared to mock group. Although the 106 PFU/mL-vaccinated and BEI-inactivated groups showed significantly greater VN titers 22 days after challenge, all the groups were already negative for viremia.Porcine reproductive and respiratory syndrome (PRRS) is an economically relevant emerging swine viral disease that was first recognized in North America in 1987 [1] and in Europe in 1990 [2]. The causative agent of this disease, PRRS virus (PRRSV), was first isolated in the Netherlands in 1990 [2] and was designated Lelystad virus (LV). Subsequently, the same agent causing PRRS was also identified in the United States [3]. PRRSV is an enveloped single-stranded RNA virus of the Arteriviridae family, a member of the order Nidovirales [4].PRRSV can cause severe reproductive failure in sows that is characterized by late-term abortion, stillbirth, and the birth of weak piglets; it is also associated with porcine respiratory disease complex in combination with secondary infections [5,6]. In the US alone, the economic losses caused by PRRS amount to more than US $560 million annually, and it is the most significant infecti
First Evaluation of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency in Vivax Malaria Endemic Regions in the Republic of Korea
Youn-Kyoung Goo, So-Young Ji, Hyun-Il Shin, Jun-Hye Moon, Shin-Hyung Cho, Won-Ja Lee, Jung-Yeon Kim
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0097390
Abstract: Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common human enzyme defect and affects more than 400 million people worldwide. This deficiency is believed to protect against malaria because its global distribution is similar. However, this genetic disorder may be associated with potential hemolytic anemia after treatment with anti-malarials, primaquine or other 8-aminoquinolines. Although primaquine is used for malaria prevention, no study has previously investigated the prevalence of G6PD variants and G6PD deficiency in the Republic of Korea (ROK). Methods Two commercialized test kits (Trinity G-6-PDH and CareStart G6PD test) were used for G6PD deficiency screening. The seven common G6PD variants were investigated by DiaPlexC kit in blood samples obtained living in vivax malaria endemic regions in the ROK. Results Of 1,044 blood samples tested using the CareStart G6PD test, none were positive for G6PD deficiency. However, a slightly elevated level of G6PD activity was observed in 14 of 1,031 samples tested with the Trinity G-6-PDH test. Forty-nine of the 298 samples with non-specific amplification by DiaPlexC kit were confirmed by sequencing to be negative for the G6PD variants. Conclusions No G6PD deficiency was observed using phenotypic- or genetic-based tests in individuals residing in vivax malaria endemic regions in the ROK. Because massive chemoprophylaxis using primaquine has been performed in the ROK military to kill hypnozoites responsible for relapse and latent stage vivax malaria, further regular monitoring is essential for the safe administration of primaquine.
Curcumin Attenuates Acute Graft-versus-Host Disease Severity via In Vivo Regulations on Th1, Th17 and Regulatory T Cells
Min-Jung Park, Su-Jin Moon, Sung-Hee Lee, Eun-Ji Yang, Jun-Ki Min, Seok-Goo Cho, Chul-Woo Yang, Sung-Hwan Park, Ho-Youn Kim, Mi-La Cho
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067171
Abstract: Background In this study we examined the in vivo and in vitro effects and mechanisms of action of curcumin on the development of acute graft-versus-host disease (GVHD) using a murine model. Methodology/Principal Findings Mixed lymphocyte reactions were used to determine the in vitro effects of curcumin. Treatment with curcumin attenuated alloreactive T cell proliferation and inhibited the production of interferon (IFN)-γ and interleukin (IL)-17. In a murine acute GVHD model, transplantation of curcumin-treated allogeneic splenocytes into irradiated recipient mice significantly reduced the clinical severity scores of acute GVHD manifested in the liver, skin, colon and lung as compared with animals receiving vehicle-treated splenocytes. c-Fos and c-Jun expression levels in the skin and intestine, which are major target organs, were analyzed using immunohistochemical staining. Expression of both proteins was reduced in epithelial tissues of skin and intestine from curcumin-treated GVHD animals. The IFN-γ-expressing CD4+ splenocytes and IFN-γ-expressing lymph node cells were dramatically decreased in curcumin-treated mice. In contrast, CD4+Foxp3+ splenocytes were increased in the curcumin-treated acute GVHD animals. Flow cytometric analysis revealed that animals transplanted with curcumin-treated allogeneic splenocytes showed increased populations of CD4+ regulatory T cells (Tregs) as well as CD8+ Treg cells, compared to animals administered vehicle-treated splenocytes. Curcumin-treated acute GVHD animals could have a change in B cell subpopulations. Conclusion/Significance In the present study, we investigated the efficacy and mechanism of action of curcumin treatment against acute GVHD. The acute GVHD mice administered with curcumin-treated splenocytes showed significantly reduced severity of acute GVHD. Curcumin exerted in vivo preventive effects on acute GVHD by reciprocal regulation of T helper 1 (Th1) and Treg (both CD4+ and CD8+ Treg) cell lineages as well as B cell homeostasis.
Comparisons of temperament and character between problematic internet users and problematic drug users in Korean adolescents  [PDF]
Moon-Soo Lee, In-Kwa Jung
Open Journal of Psychiatry (OJPsych) , 2012, DOI: 10.4236/ojpsych.2012.23030
Abstract: Objective: It has been suggested that some internet users spend so much time on the internet that their personal and/or professional lives suffer. This study compared the temperament and character of problematic internet users (PIU) with that of problematic drug users (PDU) among Korean adolescents and aimed to examine the personality characteristics of adolescents with internet use problems. Method: We enrolled participants from high schools (n = 487), internet cafés (n = 89), and an adolescent-substance abuse consultation office (n = 45). All subjects were assessed using the Korean Internet Addiction Scale (KIAS), the Korean Adolescent Drug Addiction Screening Test-2 (KOADAST-2), and the Korean version of the Temperament and Character Inventory (K-TCI). Subjects were classified into the following four groups based on the KIAS and KOADAST-2 results: normal controls, pure PIUs, pure PDUs, and dual problematic users. Differences in the subscale scores on the K-TCI were analyzed by group. Results: Scores for Reward dependence (RD) were significantly lower in pure PIUs than in pure PDUs (13.85 ± 4.07 versus 15.44 ± 3.56, p < 0.05). Scores for Self-Directedness (SD) were lower in pure PIUs (18.21 ± 6.90) and pure PDUs (18.22 ± 6.03) than in normal controls (20.88 ± 6.60), although the scores did not differ significantly between pure PIUs and pure PDUs. Scores for Cooperativeness (C) were lower in pure PIUs than in normal controls (23.97 ± 7.02 versus 26.57 ± 6.67, p < 0.05). Conclusions: The results of this study suggest that PIUs are more socially insensitive and that they may experience more inter-personal problems than PDUs.
Early science with Korean VLBI network: the QCAL-1 43GHz calibrator survey
Leonid Petrov,Sang-Sung Lee,Jongsoo Kim,Taehyun Jung,Junghwan Oh,Bong Won Sohn,Do-Young Byun,Moon-Hee Chung,Do-Heung Je,Seog-Oh Wi,Min-Gyu Song,Jiman Kang,Seog-Tae Han,Jung-Won Lee,Bong Gyu Kim,Hyunsoo Chung,Hyun-Goo Kim
Physics , 2012, DOI: 10.1088/0004-6256/144/5/150
Abstract: This paper presents the catalog of correlated flux densities in three ranges of baseline projection lengths of 637 sources from a 43 GHz (Q-band) survey observed with the Korean VLBI Network. Of them, 623 sources have not been observed before at Q-band with VLBI. The goal of this work in the early science phase of the new VLBI array is twofold: to evaluate the performance of the new instrument that operates in a frequency range of 22-129 GHz and to build a list of objects that can be used as targets and as calibrators. We have observed the list of 799 target sources with declinations down to -40 degrees. Among them, 724 were observed before with VLBI at 22 GHz and had correlated flux densities greater than 200 mJy. The overall detection rate is 78%. The detection limit, defined as the minimum flux density for a source to be detected with 90% probability in a single observation, was in a range of 115-180 mJy depending on declination. However, some sources as weak as 70 mJy have been detected. Of 623 detected sources, 33 objects are detected for the first time in VLBI mode. We determined their coordinates with the median formal uncertainty 20 mas. The results of this work set the basis for future efforts to build the complete flux-limited sample of extragalactic sources at frequencies 22 GHz and higher at 3/4 of the celestial sphere.
The New Role of Instructors and Curators for the First-Year Architectural Design Students in Taiwan  [PDF]
Moon-Lin Jau, Naai-Jung Shih
Art and Design Review (ADR) , 2017, DOI: 10.4236/adr.2017.51004
Abstract: Taiwan has become a renowned Chinese cultural showcase in recent years. For architectural instructors, bringing student class efforts to the public has become a part of pedagogical events and the purpose of this study. Instructors who are also designers have established a pattern of collaborating with local small galleries for organizing design exhibitions. The result is a new collaborate pedagogical pattern which enables design students to reach out to society and, in the meantime, open up a window of diversity for the galleries as test workshops for design practice. This pattern has been carried out for three years. The results not only encourage students, but also motivate them with an open-mind learning attitude in a real social space.
Finite Element Analysis of Tire Traction Using a Rubber-Ice Friction Model  [PDF]
Hyun Chul Jung, Woo Cheol Park, Kyoung Moon Jeong
Open Journal of Applied Sciences (OJAppS) , 2018, DOI: 10.4236/ojapps.2018.811040
Abstract: The friction of road surface covered by snow or ice is very low and that results in reducing vehicle traction forces and potential traffic accidents. In general, to establish a master curve on a rubber-ice friction model is difficult because the ice surface, being not far removed from its melting point, reacts itself very sen-sitively to pressure, speed, and temperature changes. In this paper, an accepta-ble frictional interaction model was implemented to finite element method to rationally examine the frictional interaction behavior on ice between the tire and the road surface. The formula of friction characteristic according to tem-perature and sliding velocity on the ice surface was applied for tire traction analysis. Numerical results were verified by comparing the outdoor test data and it was confirmed to indicate similar correlation. It is found that the rub-ber-ice friction model will be useful for the improvement of the ice traction performance of tire.
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