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Search Results: 1 - 10 of 104 matches for " Monia Pacenti "
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The Complex Epidemiological Scenario of West Nile Virus in Italy
Luisa Barzon,Monia Pacenti,Elisa Franchin,Laura Squarzon,Enrico Lavezzo,Margherita Cattai,Riccardo Cusinato,Giorgio Palù
International Journal of Environmental Research and Public Health , 2013, DOI: 10.3390/ijerph10104669
Abstract: Entomological, veterinary, and human surveillance systems for West Nile virus (WNV) infection have been implemented in Italy since the first detection of the virus in 1998. These surveillance activities documented a progressive increase of WNV activity and spread in different regions and the emergence of new WNV lineages and strains. Italy is a paradigmatic example of the complex epidemiology of WNV in Europe, where sporadic cases of WNV infection, clusters, and small outbreaks have been reported in several regions. In addition, different strains of both WNV lineage 1 and lineage 2 have been identified, even co-circulating in the same area.
Recombinant Envelope-Proteins with Mutations in the Conserved Fusion Loop Allow Specific Serological Diagnosis of Dengue-Infections
Alexandra Rockstroh?,Luisa Barzon?,Monia Pacenti,Giorgio Palù?,Matthias Niedrig?,Sebastian Ulbert
PLOS Neglected Tropical Diseases , 2015, DOI: 10.1371/journal.pntd.0004218
Abstract: Dengue virus (DENV) is a mosquito-borne flavivirus and a major international public health concern in many tropical and sub-tropical areas worldwide. DENV is divided into four major serotypes, and infection with one serotype leads to immunity against the same, but not the other serotypes. The specific diagnosis of DENV-infections via antibody-detection is problematic due to the high degree of cross-reactivity displayed by antibodies against related flaviviruses, such as West Nile virus (WNV), Yellow Fever virus (YFV) or Tick-borne encephalitis virus (TBEV). Especially in areas where several flaviviruses co-circulate or in the context of vaccination e.g. against YFV or TBEV, this severely complicates diagnosis and surveillance. Most flavivirus cross-reactive antibodies are produced against the highly conserved fusion loop (FL) domain in the viral envelope (E) protein. We generated insect-cell derived recombinant E-proteins of the four DENV-serotypes which contain point mutations in the FL domain. By using specific mixtures of these mutant antigens, cross-reactivity against heterologous flaviviruses was strongly reduced, enabling sensitive and specific diagnosis of the DENV-infected serum samples in IgG and IgM-measurements. These results have indications for the development of serological DENV-tests with improved specificity.
Report of two cases of influenza virus A/H1N1v and B co-infection during the 2010/2011 epidemics in the Italian Veneto Region
Arianna Calistri, Cristiano Salata, Marina Cosentino, Samuele Asnicar, Elisa Franchin, Riccardo Cusinato, Monia Pacenti, Isabella Donatelli, Giorgio Palù
Virology Journal , 2011, DOI: 10.1186/1743-422x-8-502
Abstract: The influenza epidemic in the 2010/2011 season has been characterized in Italy, as in the rest of the world, by a significant co-circulation of influenza A (mostly H1N1v) and influenza B viruses [1]. 1403 nasopharyngeal swabs from hospitalized patients displaying influenza-like illness underwent virological evaluation at the Microbiology and Virology Unit of the Padova Hospital, the Italian Veneto Region reference laboratory for the diagnosis of influenza and other respiratory diseases. In particular, the viral RNA was extracted from the specimens (NucliSENS? easyMAG?, Biomerieux, Lyon, France) and a standardized controlled real time Reverse Transcription Polymerase Chain Reaction (RT-PCR) was performed [2]. This method allows the detection of influenza A and B viruses and the analysis of influenza A specific sub-types. In more details, roughly 70 ng of RNA extracted from each sample were assayed with 5 different primer and probe sets specific for influenza A, influenza B, A/H1N1v, A/H3N2 and the housekeeping gene RNasi-P. Overall 327 (23, 3%) samples were positive for influenza virus. Among the positive samples 62 were positive for influenza B virus (25%) and 265 for influenza A virus (75%) with a sharp prevalence of A/H1N1v (183 samples, 69%) over the A/H3N2 (6 samples, 2.2%). It has to be mentioned that 28.8% of influenza A virus positive samples could not be sub-typed, likely due to a low efficiency of the H1N1v specific real time adopted. A similar observation was reported during last influenza season by different European laboratories, adopting this same controlled diagnostic system [1]. The age distribution shows a prevalence of positive patients in the > 60 year old category, as expected during and epidemic season of influenza. Interestingly these data, that are in line with the results reported by different laboratories in the northern hemisphere [1], show a significant co-circulation of influenza A and influenza B viruses, with in general higher prevalence
Genome Sequencing of West Nile Virus from Human Cases in Greece, 2012
Luisa Barzon,Anna Papa,Monia Pacenti,Elisa Franchin,Enrico Lavezzo,Laura Squarzon,Giulia Masi,Thomas Martello,Theodolinta Testa,Riccardo Cusinato,Giorgio Palù
Viruses , 2013, DOI: 10.3390/v5092311
Abstract: A West Nile Virus (WNV) lineage 2 strain, named Nea Santa-Greece-2010, has been demonstrated to be responsible for the large outbreaks of neuroinvasive disease (WNND) that have been occurring in Greece since 2010, based on sequence similarities of viral isolates identified between 2010–2012. However, knowledge on the evolution of this strain is scarce because only partial WNV genome sequences are available from Greece. The aim of this study was to get the complete genome sequence of WNV from patients with infection. To this aim, plasma and urine samples collected during the 2012 Greek outbreak were retrospectively investigated. Full WNV genome sequence was obtained from a patient with WNND. The genome had 99.7% sequence identity to Nea Santa, higher than to other related WNV lineage 2 strains, and five amino acid changes apparently not relevant for viral pathogenicity or fitness. In addition, infection by WNV lineage 2 was confirmed in additional nine patients with WNND; in three of them the infection with WNV Nea Santa was demonstrated by sequencing. In conclusion, this study characterized for the first time a WNV full genome from a patient with WNND from Greece, demonstrated the persistence of the Nea Santa strain, and suggested that the virus might have locally evolved.
Large Human Outbreak of West Nile Virus Infection in North-Eastern Italy in 2012
Luisa Barzon,Monia Pacenti,Elisa Franchin,Silvana Pagni,Enrico Lavezzo,Laura Squarzon,Thomas Martello,Francesca Russo,Loredana Nicoletti,Giovanni Rezza,Concetta Castilletti,Maria Rosaria Capobianchi,Pasquale Salcuni,Margherita Cattai,Riccardo Cusinato,Giorgio Palù
Viruses , 2013, DOI: 10.3390/v5112825
Abstract: Human cases of West Nile virus (WNV) disease have been reported in Italy since 2008. So far, most cases have been identified in north-eastern Italy, where, in 2012, the largest outbreak of WNV infection ever recorded in Italy occurred. Most cases of the 2012 outbreak were identified in the Veneto region, where a special surveillance plan for West Nile fever was in place. In this outbreak, 25 cases of West Nile neuroinvasive disease and 17 cases of fever were confirmed. In addition, 14 WNV RNA-positive blood donors were identified by screening of blood and organ donations and two cases of asymptomatic infection were diagnosed by active surveillance of subjects at risk of WNV exposure. Two cases of death due to WNND were reported. Molecular testing demonstrated the presence of WNV lineage 1 in all WNV RNA-positive patients and, in 15 cases, infection by the novel Livenza strain was ascertained. Surveillance in other Italian regions notified one case of neuroinvasive disease in the south of Italy and two cases in Sardinia. Integrated surveillance for WNV infection remains a public health priority in Italy and vector control activities have been strengthened in areas of WNV circulation.
Polybrominated Diphenyl Ethers and Polychlorobiphenyls in Fish from the Ionian Sea (Western Mediterranean)  [PDF]
Silvano Focardi, Monia Renzi
Journal of Environmental Protection (JEP) , 2012, DOI: 10.4236/jep.2012.32017
Abstract: This study reports on the accumulation of polybrominated diphenyl ethers (PBDEs) and polychlorobiphenyls (PCBs) in the muscle tissue of 11 species of fish from the Ionian Sea (Western Mediterranean). The results are consistent with previous studies that have reported now-generalized contamination by PCBs of the Mediterranean marine environment, as well as widespread diffusion of emerging contaminants such as polybrominated diphenyl ethers (PBDEs). The Western Mediterranean Sea, from which the 11 fish species were collected, receives a heavy pollutant input from the urban and industrial areas along its coasts. Higher values of PCBs (average over 1.5 mg/kg f.w.) were observed in pelagic top predators (little tuna, bluefin tuna and swordfish). These same species also showed higher levels of PBDEs, averaging above 0.5 ng/g f.w. This puts a few fish species at the top of the food chain—such as bluefin tuna and swordfish—at particular risk, and the importance of these species in the human diet suggests the need for particular care in our food choices.
Histoire d’un voile
Monia Mazigh
Voix Plurielles , 2012,
Abstract: Mazigh, Monia. Création
Cramer-Rao Lower Bound for NDA SNR Estimation from Linear Modulation Schemes over Flat Rayleigh Fading Channel
Monia Salem
International Journal of Computer Science Issues , 2013,
Abstract: In this contribution, Cramer-Rao lower bound (CRLB) for signal-to-noise ratio (SNR) estimation is addressed from linear modulation signals considering a transmission over a fading channel. We derived the expressions of Fisher information matrix entries to assess the optimal variance of any unbiased SNR estimator. Based on Monte Carlo integration, simulation results are drawn from several constellation densities and observation window sizes. For considered modulations, it is shown that the lower bound takes higher values as long as the modulation order increases. The derived bound provides an efficient standard for evaluating the performance of any unbiased non-data aided (NDA) SNR estimator from linear modulation signals over flat Rayleigh fading channel (FRFC).
La répartition des établissements industriels à Sfax : un schéma radio-concentrique The radioconcentric organisation of industrial establishments in Sfax
Monia Gasmi
Revue des Mondes Musulmans et de la Méditerranée , 2011, DOI: 10.4000/remmm.2879
Abstract: Si l'organisation spatiale de l'industrie dans les agglomérations des pays développés a été suffisamment explorée, celle des villes des pays en développement qui ont été engagées dans une entreprise d'industrialisation n'a pas été, à notre connaissance, le sujet d'une étude géogra-phique quantitative. Mobilisant les techniques de l'analyse spatiale qui ont été peu appliquées jusqu'ici sur des terrains de pays dits émergents comme la Tunisie, nous avons tenté, à travers l'exemple de Sfax, d'analyser la répartition des établissements industriels. Les résultats mettent en évidence l'existence d'un schéma d'organisation radio-concentrique, largement dépassé, depuis les années cinquante dans les pays du Nord. L'étude des distributions des industries, thème privilégié de cet article et sans conteste problématique ancienne de la géographie, permet de combler les lacunes de la littérature géographique en Tunisie. Elle fournit des représentations visuelles et argumentées qui sont indispensables pour tous ceux qui ont à prendre des décisions ayant des incidences géographiques, qu'il s'agisse des chefs d'entreprises, des responsables de collectivités locales ou d'acteurs de l'aménagement. While the spatial organization of metropolitan industry in developed countries has been widely studied, cities in developing countries engaged in a process of industrialization, have not, to the best of our knowledge, benefited from similar, quantitative geographical studies. Making use of techniques infrequently applied in the study of developing economies, we have attempted to analyse the geographical distribution of industrial establishments in Sfax, Tunisia. The study reveals the existence of an archaic radioconcentric model in use among northern countries prior to the 1950's. The study of industrial space allocation, the main topic of our article and a classical problem of economic geography, makes it possible to close the gaps in the literature of Tunisian industrial geography. It provides essential visual and analytical support for decisions of geographical import, whether for company management, urban planners or developers.
Science is not for me. Visitors’ attitudes to learning in an Italian science centre
Monia Cardella
JCOM : Journal of Science Communication , 2006,
Abstract: Following the example of the Exploratorium in San Francisco, interactive science museums are meant to be informal and enjoyable places where visitors, regardless of their age and background, are stimulated to practice their abilities to explore the world from a scientific point of view or to reacquire it in the case of adults who are far from science for professional reasons. Our study, which belongs to a relatively recent, but increasingly richer and complex tradition of researches on this topic, aims at contributing to answering the question whether, within the context of hands-on museums, this desired reacquisition of scientific exploration actually occurs for all visitors; more precisely, it aims at contributing to the discussion resulting from this question with reference to both possible answers and methods to find them. The study described below was carried out for a Science Communication Master thesis in Trieste (student: Monia Cardella, supervisor: Paola Rodari) and, therefore, it is inevitably limited: in fact, in order to deal with such a complex issue and to perform more detailed investigations on the field longer time and more resources would have been necessary. However, both methods used and results obtained from it, although provisional, are significant enough to deserve our attention.
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