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Search Results: 1 - 10 of 3823 matches for " Mirko Lo Cicero "
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New Macrocyclic Amines Showing Activity as HIV Entry Inhibitors Against Wild Type and Multi-Drug Resistant Viruses
Stefano Rusconi,Mirko Lo Cicero,Ottavia Viganò,Francesca Sirianni,Elisabetta Bulgheroni,Stefania Ferramosca,Andrea Bencini,Antonio Bianchi,Lidia Ruiz,Cecilia Cabrera,Javier Martinez-Picado,Claudiu T. Supuran,Massimo Galli
Molecules , 2009, DOI: 10.3390/molecules14051927
Abstract: Considering as a lead molecule the chemokine CXCR4 receptor antagonist AMD-3100, which shows significant anti-HIV activity in vitro and in vivo, we investigated a series of structurally related macrocyclic polyamines incorporating o,o’-phenanthroline or 2,2’-bipyridyl scaffolds as potential antiviral agents with lower toxicity and increased activity against both wild type X4-tropic and dual tropic HIV strains. The antiviral activity of these compounds was evaluated by susceptibility assays in PBMC (Peripheral Blood Mononuclear Cells) and compared to that of AMD-3100. The newly investigated compounds showed IC50s values in the low micromolar range and significantly inhibited the viral replication of wild type X4-tropic isolate and dual tropic strains. These macrocyclic polyamines constitute a promising class of HIV entry inhibitors.
A practical approach for the validation of sterility, endotoxin and potency testing of bone marrow mononucleated cells used in cardiac regeneration in compliance with good manufacturing practice
Sabrina Soncin, Viviana Lo Cicero, Giuseppe Astori, Gianni Soldati, Mauro Gola, Daniel Sürder, Tiziano Moccetti
Journal of Translational Medicine , 2009, DOI: 10.1186/1479-5876-7-78
Abstract: For the potency assay, cells were placed in the upper part of a modified Boyden chamber containing Endocult Basal Medium with supplements and transmigrated cells were scored. The invasion index was expressed as the ratio between the numbers of invading cells relative to cell migration through a control insert membrane.For endotoxins, we used a commercially available system based on the kinetic chromogenic LAL-test. Validation of sterility was performed by direct inoculation of TSB and FTM media with the cell product following Eu Ph 2.6.1 guideline.The calculated MVD and endotoxin limit were 780× and 39 EU/ml respectively. The 1:10 and 1:100 dilutions were selected for the validation. For sterility, all the FTM cultures were positive after 3 days. For TSB cultures, Mycetes and B. subtilis were positive after 5 and 3 days respectively. The detection limit was 1-10 colonies.A total of four invasion assay were performed: the calculated invasion index was 28.89 ± 16.82% (mean ± SD).We have validated a strategy for endotoxin, sterility and potency testing in an ATMP used in cardiac regeneration. Unlike pharmaceutical products, many stem-cell-based products may originate in hospitals where personnel are unfamiliar with the applicable regulations. As new ATMPs are developed, the regulatory framework is likely to evolve. Meanwhile, existing regulations provide an appropriate structure for ensuring the safety and efficacy of the next generation of ATMPs. Personnel must be adequately trained on relevant methods and their application to stem-cell-based products.The European Union (EU) regulation on advanced therapy medicinal products [1] (ATMP) is entered into force in all European Member States on December 30, 2008, and Food and Drug Administration (FDA) recently promulgated regulations on human cells, tissues, and cellular and tissue-based products [2] issuing an appropriate regulatory structure for the wide range of stem-cell-based products that may be developed to regenerat
Terbinafine versus itraconazole: a long-term, randomized, double-blind, clinical trial in chronic pulmonary aspergillosis. A pilote study
"Gianfranco Schiraldi", "Salvatore Lo Cicero", "Claudio Rossetti","Delia Colombo", "Michele Chiericozzi", "Fabio Colombo", "Maurizio Ferrarese", "Elvana Kola"," Luigi Allegra"
Journal of Health and Social Sciences , 3, DOI: 10.19204/2016/trbn7
Abstract: Background: The frequency of pulmonary aspergillosis has been increasing for decades. Standard oral treatment is associated with tolerability problems and interactions with commonly used medications. Present data suggest that terbinafine may be a useful alternative for chronic forms. Methods: A randomised, double-blind, multicentre trial compared terbinafine (500 mg b.i.d) with itraconazole (200 mg b.i.d.) with respect to successful outcomes for up to 6 months in 24 patients with chronic pulmonary aspergillosis. The primary end-point was a global clinical assessment using a 4-point rating scale, including (thorax) X-ray or Tc features. Secondary end-points were mycological response, duration of treatment and tolerability based on adverse events, haematology, biochemistry and urinalyses. Results: The patients were randomly allocated to terbinafine or itraconazole. No patients discontinued terbinafine, while 2 of 12 patients discontinued itraconazole because of protocol violation (n=1) or withdrawal of consent (n=1). The mean ± SD duration of the treatment was longer in the terbinafine group (139.8 ± 43.6 days vs 120.5 ± 41.8 days). The clinical success rate was also higher with terbinafine (91.7% vs 70.0%), as was the eradication rate (100% vs 75%). The proportion of patients who reported adverse events was higher with itraconazole (7 of 12 vs 3 of 12). Conclusions: Terbinafine (500 mg b.i.d. for up to 6 months) is an effective, well-tolerated antifungal agent for the treatment of chronic infectious forms of pulmonary aspergillosis, representing a valid alternative to treatment with itraconazole in these clinical forms, due to better clinical efficacy and safety and lower costs vs new azoles.
Representa??o, retrato e drama
Araujo, Cicero;
Lua Nova: Revista de Cultura e Política , 2006, DOI: 10.1590/S0102-64452006000200008
Abstract: the article elaborates on two artistic metaphors to search for contrasting modes of thinking on political representation. the metaphors suggest two very distinctive ideals of the good political order - despite a certain trend of the traditional political lexicon to mix them up - and make their own impacts on what to expect from the authorities? practices of representation.
Apresenta o
Araujo Cicero
Lua Nova: Revista de Cultura e Política , 2003,
On the second Hamming weight of some Reed-Muller type codes
Cicero Carvalho
Mathematics , 2013,
Abstract: We study affine cartesian codes, which are a Reed-Muller type of evaluation codes, where polynomials are evaluated at the cartesian product of n subsets of a finite field F_q. These codes appeared recently in a work by H. Lopez, C. Renteria-Marquez and R. Villareal and, in a generalized form, in a work by O. Geil and C. Thomsen. Using methods from Gr\"obner basis theory we determine the second Hamming weight (also called next-to-minimal weight) for particular cases of affine cartesian codes and also some higher Hamming weights of this type of code.
The effect of bisphosphonates on gene expression: GAPDH as a housekeeping or a new target gene?
Maria Valenti, Francesco Bertoldo, Luca Dalle Carbonare, Giuseppe Azzarello, Sonia Zenari, Mirko Zanatta, Elena Balducci, Orazio Vinante, Vincenzo Lo Cascio
BMC Cancer , 2006, DOI: 10.1186/1471-2407-6-49
Abstract: We evaluated GAPDH gene expression by real time RT PCR in breast (MCF-7 and T47D) and prostate (PC3 and DU-145) cancer cell lines treated with amino and non-amino bisphosphonates.Our results showed that amino-bisphosphonates significantly decrease in a dose-dependent manner the expression of GAPDH gene.Therefore, GAPDH is inaccurate to normalize mRNA levels in studies investigating the effect of bisphosphonates on gene expression and it should be avoided. On the other hand, this gene could be considered a potential target to observe the effects of bisphosphonates on cancer cells.GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is well known for its glycolytic function of converting D-Glyceraldeide-3-phosphate to 1,3-bisphosphoglycerate and it has been commonly considered as a constitutive housekeeping gene. It is widely used as a control RNA in Northern Blotting and in RT-PCR analysis and recently in real time RT-PCR.In some experimental systems its expression is constant at different times and after experimental manipulation [1]. In breast cancer cells treated with endoxifen GAPDH was used to normalize the expression data of the progesterone receptor mRNA [2]. In addition, GAPDH was the best control gene in the apoptosis pattern on the myeloid cell lines incubated with Camptothecin investigated by real time RT-PCR [3].However, there is overwhelming evidence suggesting that its use as an internal standard is inappropriate [4]. Growth hormone, oxidative stress and the tumour suppressor TP53 have all been shown to activate its transcription, which can also be induced in endothelial cells [5]. Conversely, retinoic acid down-regulates GAPDH transcription in adipocytes [6]. Furthermore, it has been observed that GAPDH mRNA expression was not normal in some tumour samples and its distribution exhibited a wide range of values. GAPDH mRNA was over-expressed in the poorly differentiated BT-20 cell line and the treatment of these cells with 1,25-dihydroxyvitamin D3 (1,25-(OH)
Multiple Sclerosis in the Mount Etna Region: Possible Role of Volcanogenic Trace Elements
Alessandra Nicoletti, Elisa Bruno, Martina Nania, Edoardo Cicero, Silvia Messina, Clara Chisari, Josita Torrisi, Davide Maimone, Roberto Marziolo, Salvatore Lo Fermo, Francesco Patti, Salvatore Giammanco, Mario Zappia
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0074259
Abstract: Background Trace elements have been hypothesised to be involved in the pathogenesis of Multiple Sclerosis and volcanic degassing is the major natural sources of trace elements. Both incidence of Multiple Sclerosis in Catania and volcanic activity of Mount Etna have been significantly increased during the last 30 years. Due to prevailing trade winds direction, volcanic gases from Etna summit craters are mostly blown towards the eastern and southern sectors of the volcano. Objective To evaluate the possible association between Multiple Sclerosis and exposure to volcanogenic trace elements. Methods We evaluated prevalence and incidence of Multiple Sclerosis in four communities (47,234 inhabitants) located in the eastern flank and in two communities (52,210 inhabitants) located in the western flank of Mount Etna, respectively the most and least exposed area to crater gas emissions. Results A higher prevalence was found in the population of the eastern flank compared to the population of the western one (137.6/100,000 versus 94.3/100,000; p-value 0.04). We found a borderline significantly higher incidence risk during the incidence study period (1980–2009) in the population of the eastern flank 4.6/100,000 (95% CI 3.1–5.9), compared with the western population 3.2/100,000 (95% CI 2.4–4.2) with a RR of 1.41 (95% CI 0.97–2.05; p-value 0.06). Incidence risks have increased over the time in both populations reaching a peak of 6.4/100,000 in the eastern flank and of 4.4/100.000 in the western flank during 2000–2009. Conclusion We found a higher prevalence and incidence of Multiple Sclerosis among populations living in the eastern flank of Mount Etna. According to our data a possible role of TE cannot be ruled out as possible co-factor in the MS pathogenesis. However larger epidemiological study are needed to confirm this hypothesis.
APEnet+: high bandwidth 3D torus direct network for petaflops scale commodity clusters
Roberto Ammendola,Andrea Biagioni,Ottorino Frezza,Francesca Lo Cicero,Alessandro Lonardo,Pier Stanislao Paolucci,Davide Rossetti,Andrea Salamon,Gaetano Salina,Francesco Simula,Laura Tosoratto,Piero Vicini
Physics , 2011, DOI: 10.1088/1742-6596/331/5/052029
Abstract: We describe herein the APElink+ board, a PCIe interconnect adapter featuring the latest advances in wire speed and interface technology plus hardware support for a RDMA programming model and experimental acceleration of GPU networking; this design allows us to build a low latency, high bandwidth PC cluster, the APEnet+ network, the new generation of our cost-effective, tens-of-thousands-scalable cluster network architecture. Some test results and characterization of data transmission of a complete testbench, based on a commercial development card mounting an Altera FPGA, are provided.
Architectural improvements and 28 nm FPGA implementation of the APEnet+ 3D Torus network for hybrid HPC systems
Roberto Ammendola,Andrea Biagioni,Ottorino Frezza,Francesca Lo Cicero,Pier Stanislao Paolucci,Alessandro Lonardo,Davide Rossetti,Francesco Simula,Laura Tosoratto,Piero Vicini
Physics , 2013, DOI: 10.1088/1742-6596/513/5/052002
Abstract: Modern Graphics Processing Units (GPUs) are now considered accelerators for general purpose computation. A tight interaction between the GPU and the interconnection network is the strategy to express the full potential on capability computing of a multi-GPU system on large HPC clusters; that is the reason why an efficient and scalable interconnect is a key technology to finally deliver GPUs for scientific HPC. In this paper we show the latest architectural and performance improvement of the APEnet+ network fabric, a FPGA-based PCIe board with 6 fully bidirectional off-board links with 34 Gbps of raw bandwidth per direction, and X8 Gen2 bandwidth towards the host PC. The board implements a Remote Direct Memory Access (RDMA) protocol that leverages upon peer-to-peer (P2P) capabilities of Fermi- and Kepler-class NVIDIA GPUs to obtain real zero-copy, low-latency GPU-to-GPU transfers. Finally, we report on the development activities for 2013 focusing on the adoption of the latest generation 28 nm FPGAs and the preliminary tests performed on this new platform.
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