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Search Results: 1 - 10 of 72603 matches for " Ming-Bo Huang "
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Nef-M1, a CXCR4 Peptide Antagonist, Enhances Apoptosis and Inhibits Primary Tumor Growth and Metastasis in Breast Cancer  [PDF]
Harvey Bumpers, Ming-Bo Huang, Venkat Katkoori, Upender Manne, Vincent Bond
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.44101

Results from studies with animal models suggest that, in many cancers, CXCR4 is an important therapeutic target and that CXCR4 antagonists may be promising treatments for primary cancers and for metastases. The Nef protein effectively competes with CXCR4’s natural ligand, SDF-1α, and induces apoptosis. As described in this report, the Nef-M1 peptide (Nef protein amino acids 50 - 60) inhibits primary tumor growth and metastasis of breast cancer (BC). Four BC cell lines (MDA-MB-231, MDA-MB-468, MCF 7, and DU4475) and primary human mammary epithelium (HME) cells were evaluated for their response to the Nef protein and to the Nef-M1 peptide. The presence of CXCR4 receptors in these cells was determined by RT-PCR, Western blot (WB), and immunohistochemical analyses. The apoptotic effect of Nef-M1 was assessed by terminal transferase dUTP nick-end labeling (TUNEL). WBs was used to assess caspase 3 activation. BC xenografts grown in SCID mice were evaluated for the presence of CXCR4 and for their metastatic potential. CXCR4 was presented in MDA-MB-231, MCF 7, and DU 4475 BC cells but not in MDA-MB-468 BC or HME cells. Cells expressing CXCR4 and treated with Nef-M1 peptide or the Nef protein had higher rates of apoptosis than untreated cells. Caspase-3 activation increased in MDA-MB 231 cells treated with the Nef protein, the Nef 41 - 60 peptide, or Nef-M1. Nef-M1, administered to mice starting at the time of xenograft implantation, inhibited growth of primary tumors and metastatic spread. Untreated mice developed diffuse intraperitoneal metastases. We conclude that, in BCs, Nef-M1, through interaction with CXCR4, inhibits primary tumor growth and metastasis by causing apoptosis.

Small RNAs and Transgenerational Epigenetic Variation  [PDF]
Joanne R. M. Lee, Zane Duxbury, Ming-Bo Wang
American Journal of Plant Sciences (AJPS) , 2013, DOI: 10.4236/ajps.2013.43082

Small RNAs are found in eukaryotes and are responsible for regulation of chromatin structure, RNA processing and stability, translation and transcription. 24-nt small interfering RNA (siRNA) are known to mediate gene inactivation via the RNA-directed DNA methylation pathway (RdDM) and are important for natural heritable changes in plant species. DNA cytosine methylation can be maintained between generations and this may be important for accelerated adaption to stress conditions. Research is currently focused toward the epigenetic response to disease, the stability of DNA methylation over generations, the elucidation of newly discovered pathways for de novo DNA methylation, and the application of epigenetic variation to breeding programs. This review aims to give a brief but comprehensive examination on small RNAs and transgenerational epigenetic variation.

Characterization of Exosomes in Plasma of Patients with Breast, Ovarian, Prostate, Hepatic, Gastric, Colon, and Pancreatic Cancers  [PDF]
Ming-Bo Huang, Meng Xia, Zhao Gao, Hu Zhou, Min Liu, Shan Huang, Rong Zhen, Jennifer Y. Wu, William W. Roth, Vincent C. Bond, Jian Xiao, Jing Leng
Journal of Cancer Therapy (JCT) , 2019, DOI: 10.4236/jct.2019.105032
Abstract: Detection of circulating tumor-specific DNA, RNA or proteins can be difficult due to relative scarcity. Exosomes are extracellular vesicles, 30 - 150 nm in diameter derived from fusion of multivesicular bodies with the plasma membrane. They are composed of a lipid bilayer membrane and contain proteins, mRNA and miRNA. Exosomes are secreted by multiple cell types, including cancer cells. However, there is a relative lack of information concerning the contents of exosomes secreted by various tumor cell types. To examine exosomes in cancer, we collected blood plasma samples from patients with breast, ovarian, prostate, hepatic, gastric, colon, and pancreatic cancers. Exosomes were isolated from plasma and confirmed by AchE assay, transmission electron microscopy and expression of the CD63 exosomal marker. Expression of AFP, CA724, CA153, CEA, CA125, CA199 and PSA antigens were determined using an automated electro-chemiluminescence assay. Expression of the tumor-related chaperone protein, mortalin, was determined by Western blot analysis. Levels of exosome secretion were variable among the different tumor types. Both exosome levels and mortalin expression within tumor cell exosomes were higher than in healthy donors, except in pancreatic carcinoma, where exosomes were elevated but mortalin expression was not significantly different from healthy donors. Exosomes provide unique opportunities for the enrichment of tumor-specific materials and may be useful as biomarkers and
Cryogenic thermoelectric properties of BiTe-based alloys and cryo-energy power generation

Jiang Ming-Bo,Wu Zhi-Xiong,Zhou Min,Huang Rong-Jin,Li Lai-Feng,

物理学报 , 2010,
Abstract: 利用机械合金化和冷压烧结法制备得到n型和p型Bi2Te3基热电材料,在80—300 K温度范围测量了电导率、Seebeck系数,结果表明其具有良好的低温热电性能.采用Bi2Te3基热电材料制备出半导体热电器件,并配合附属设备搭建出一套半导体温差发电装置.利用液氮汽化时释放的冷能,对半导体热电器件的发电性能进行实验研究,得出这种半导体热电器件输出电压、输出功率与电流关系式,测得最大的输出功率达到1.33 W,从而证明了冷
Viral Small Interfering RNAs Target Host Genes to Mediate Disease Symptoms in Plants
Neil A. Smith,Andrew L. Eamens,Ming-Bo Wang
PLOS Pathogens , 2011, DOI: 10.1371/journal.ppat.1002022
Abstract: The Cucumber mosaic virus (CMV) Y-satellite RNA (Y-Sat) has a small non-protein-coding RNA genome that induces yellowing symptoms in infected Nicotiana tabacum (tobacco). How this RNA pathogen induces such symptoms has been a longstanding question. We show that the yellowing symptoms are a result of small interfering RNA (siRNA)-directed RNA silencing of the chlorophyll biosynthetic gene, CHLI. The CHLI mRNA contains a 22-nucleotide (nt) complementary sequence to the Y-Sat genome, and in Y-Sat-infected plants, CHLI expression is dramatically down-regulated. Small RNA sequencing and 5′ RACE analyses confirmed that this 22-nt sequence was targeted for mRNA cleavage by Y-Sat-derived siRNAs. Transformation of tobacco with a RNA interference (RNAi) vector targeting CHLI induced Y-Sat-like symptoms. In addition, the symptoms of Y-Sat infection can be completely prevented by transforming tobacco with a silencing-resistant variant of the CHLI gene. These results suggest that siRNA-directed silencing of CHLI is solely responsible for the Y-Sat-induced symptoms. Furthermore, we demonstrate that two Nicotiana species, which do not develop yellowing symptoms upon Y-Sat infection, contain a single nucleotide polymorphism within the siRNA-targeted CHLI sequence. This suggests that the previously observed species specificity of Y-Sat-induced symptoms is due to natural sequence variation in the CHLI gene, preventing CHLI silencing in species with a mismatch to the Y-Sat siRNA. Taken together, these findings provide the first demonstration of small RNA-mediated viral disease symptom production and offer an explanation of the species specificity of the viral disease.
Molecular Functions of Long Non-Coding RNAs in Plants
Qian-Hao Zhu,Ming-Bo Wang
Genes , 2012, DOI: 10.3390/genes3010176
Abstract: The past decade has seen dramatic changes in our understanding of the scale and complexity of eukaryotic transcriptome owing to the discovery of diverse types of short and long non-protein-coding RNAs (ncRNAs). While short ncRNA-mediated gene regulation has been extensively studied and the mechanisms well understood, the function of long ncRNAs remains largely unexplored, especially in plants. Nevertheless, functional insights generated in recent studies with mammalian systems have indicated that long ncRNAs are key regulators of a variety of biological processes. They have been shown to act as transcriptional regulators and competing endogenous RNAs (ceRNAs), to serve as molecular cargos for protein re-localization and as modular scaffolds to recruit the assembly of multiple protein complexes for chromatin modifications. Some of these functions have been found to be conserved in plants. Here, we review our current understanding of long ncRNA functions in plants and discuss the challenges in functional characterization of plant long ncRNAs.
Selection Oriented Database Data Distribution Strategy for Cloud Computing

WEN Ming-bo,DING Zhi-ming,

计算机科学 , 2010,
Abstract: Many methods have been proposed to satisfy the needs of massive data processing, among which cloud compuling is an outstanding one. The main thought of Cloud computing is using large number of PCs to compose a huge cluster as a server. With the development of Cloud Computing technology, more and more applications will turn into Cloud, including the DBMS. As Database system requires ACID, when it comes to data distribution, some operations'performance may decline, such as joins. In this paper we proposed a Selection Oriented data Distribution strategy(SOD) to improve the performance of DBMS in Cloud Computing, which works well through the experiments.


自动化学报 , 2002,
Abstract: Using the modern time series analysis method and based on the ARMA innovation model, unified and universal Wiener state filters are presented for systems with quasi-white noise and quasi-correlated noises and with measurement delay, which can handle the state filtering, smoothing and prediction problems in a unified framework. Compared to the Kalman filtering method and polynomial method,the solution to Riccati equations and Diophantine equations is avoided. A simulation example shows their effectiveness.

HE Ming-bo,HU Xing-zhou,

高分子学报 , 1989,
Abstract: Thermal peroxidized PP films and PE films were graft copolymerized with a light stabilizer,2,2,6,6-tera-methyl-piperidinyl-merhacrylate (MTMP), by UV-irradiation in the absence of sensitizers in order to improve the photostability of films. The influence of various factors, including the thermal peroxidizing time, irradiation time, monomer concentration and the type of solvents, on the grafting content of the peroxidized films was examined. It is shown that the graft copolymerization can not take place in common organic solvents except ketones, and the photostability of either grafted PP or grafted PE film increases greatly compared with that of the ungrafted film.
Study on kinetic model of hydrothermal deactivation and model of unit balance activity for catalytic cracking catalyst

REN Jie,DENG Ming-bo,

燃料化学学报 , 2004,
Abstract: The goals of this paper are to establish the kinetic model of hydrothermal deactivation and the model of unit balance activity for Fluid Catalytic Cracking catalyst. At first, considering catalytic cracking reaction as a second-order reaction, the catalyst activity relating to hydrothermal deactivation is expressed as the ratio of cracking reaction rate for hydrothermal aged catalyst to cracking reaction rate for new catalyst. The kinetic model equations of hydrothermal deactivation for self-resistance are determined by considering that the catalyst hydrothermal deactivating is accompanied with the catalyst ultra-stabilization. Then, the hydrothermal deactivation kinetic models for self-resistance are developed through parameter estimations based on the steam aging experimental data of FCC catalyst. The results of statistics analysis show that first order deactivation kinetic model with second order self-resistance factor has higher precision. In other way, based on the flow property of complete stirred-tank reactor, the model of Fluid Catalytic Cracking Unit balance activity is developed. The study points out that the observed data of micro-reaction activity for FCCU balance catalyst is conformable with the calculated data with the model of unit balance activity. The prediction results of the balance activity model show that the micro-reaction activity of the FCCU balance catalyst increases quickly firstly, then increases slowly along with catalyst consumption. In the end, higher the regenerator temperature is or larger the catalyst content of regenerator is, the lower the micro-reaction activity of FCCU balance catalyst is.
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