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Search Results: 1 - 10 of 14191 matches for " Michereff-Filho "
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Use of serological techniques for determination of Spodoptera frugiperda(J E Smith) predators (Lepidoptera: Noctuidae)
Santos-Neto, Joaquim R;Mezencio, Jose M S;Chagas, Aline T A;Michereff-Filho, Miguel;Serr?o, José E;
Neotropical Entomology , 2010, DOI: 10.1590/S1519-566X2010000300016
Abstract: spodoptera frugiperda (j e smith) is an important pest of several crops, but especially on maize in brazil. the implementation of biological control measures hinges on the identification of its predators and other natural enemies. as a means of identifying predators, antibodies against s. frugiperda eggs were generated by inoculating rabbits with macerated s. frugiperda eggs, and the production of antibodies against s. frugiperda egg proteins was verifi ed by double immunodiffusion (did). these antibodies were then utilized in another serological technique, counterimmunoeletrophoresis (cie), to identify insects that could have ingested s. frugiperda eggs. macerates of entire insects collected in maize plantations and of individual parts of their digestive tract, including the crop, were the source of antigens in the cie, while predators fed s. frugiperda eggs in the laboratory served as the control. antibodies produced by the inoculated rabbits were effective in detecting s. frugiperda egg proteins, especially if crop macerates were used as antigens. among the species of insects collected from maize plantations, lagria villosa fabricius (coleoptera: lagriidae) and a species of lygaeidae (hemiptera) were identified as possible s. frugiperda predators.
Resposta à mosca-branca (Bemisia tabaci) e ao Tomato severe rugose virus de acessos de Solanum subgênero Leptostemonum
Michereff-Filho, Miguel;Machini, Wesley DB;Mendon?a, José L;Fonseca, Maria Esther de N;Fernandes-Acioli, Niday AN;Boiteux, Leonardo S;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000300014
Abstract: the whitefly (bemisia tabaci) and the infection by begomovirus species are two major problems affecting yield and quality of the tomato (solanum lycopersicum) crop as well as other solanaceae species of economic importance. the present work was conducted aiming to characterize the reaction of 36 accessions of the genus solanum subgenus leptostemonum (= spiny solanum species) and closely related species to tomato severe rugose virus (tosrv) and b. tabaci. seedlings of the accessions (43 days after sowing) were exposed under greenhouse conditions to viruliferous whiteflies (b. tabaci biotype b) carrying an isolate of tosrv. two susceptible tomato cultivars were used as susceptible controls. reaction to the virus was evaluated using a symptom severity scale and the systemic tosrv infection was evaluated via pcr with universal begomovirus primers. a group of accessions from s. stramonifolium, s. asperolanatum, and s. jamaiscense displayed mild symptoms and low virus accumulation. the accession s. mammosum 'cnph 035', even though tolerant, was the only one displaying clear tosrv symptoms and conspicuous systemic spread of the virus. the remaining accessions were found to be free of tosrv symptoms and with no indication of systemic infection. this germplasm collection was also evaluated to b. tabaci in a free-choice assay under greenhouse conditions. significant differences were observed for the number of eggs and number of 4th instar nymphs. a group of ten accessions from s. asperolanatum, s. stramonifolium, s. paniculatum, and s. syssimbrifolium displayed no signs of whitefly infestation. therefore, accessions of the subgenus leptostemonum might represent potential sources of resistance genes to both b. tabaci and tosrv. this genetic diversity might be transferred to other solanum species via conventional and/or transgenic approaches. our results also indicated that these spiny solanum species might have minor importance either as reservoirs of begomovirus or as alterna
Comportamento de acasalamento do bicho-mineiro-do-cafeeiro, Leucoptera coffeella (Guérin-Mèneville) (Lepidoptera: Lyonetiidae)
Michereff, Mirian F.F.;Michereff Filho, Miguel;Vilela, Evaldo F.;
Neotropical Entomology , 2007, DOI: 10.1590/S1519-566X2007000300005
Abstract: despite the importance of leucoptera coffeella (guérin-mèneville) in coffee production worldwide, there is a lack of information on its reproductive biology. this knowledge will help in mass rearing, as well as support the development of behavioral control techniques for this insect. the purpose of the study was to determine the periodicity of mating and male capture and describe the mating behavior l. coffeella. in laboratory, we observed the periodicity of mating with virgin couples of different ages, zero to five days after emergence. male activity was studied in a 0.7 ha coffee plantation, cv. catuaí, where delta traps were installed at 0.5 m above ground, using either virgin females or rubber septa lured with the synthetic sex pheromone. the sequence of mating behavior was studied by making visual observations and recorded of pairs placed on individual plastic tubes. mating occurred between 4h and 6h of photophase, when the highest frequencies involved pairs with ages of one and three days after emergence, with peak of mating occurring in 5th hour of photophase. the young or old pairs showed significantly copulation frequency and the peak of matings advance in 1h. the highest male capture occurred at 12p.m. and 13 p.m. by traps with virgin females or traps with synthetic sex pheromone lures, respectively. l. coffeella is one insect with diurnal mating and the mating behavior was not different from what is know for other lepidoptera species.
Uso do ferom?nio sexual sintético para captura de machos da tra?a-das-crucíferas
MICHEREFF, MIRIAN FERNANDES FURTADO;VILELA, EVALDO FERREIRA;MICHEREFF FILHO, MIGUEL;MAFRA-NETO, AGENOR;
Pesquisa Agropecuária Brasileira , 2000, DOI: 10.1590/S0100-204X2000001000002
Abstract: the aim of this work was to study the attractiveness of males of plutella xylostella l. (lepidoptera: yponomeutidae) to different formulations of synthetic sex pheromone. the treatments were: 1) commercial formulation; 2) z11,16:ald; 3) z11,16:ac; 4) binary blends 7:3 of 2+3; 5) binary blends 5:5 of 2+3; 6) rubber septa with hexane (control); 7) five virgin females. the commercial formulation of the synthetic sex pheromone was more attractive to males, and did not differ significantly from virgin females and binary blends 5:5 treatments. the components alone were less attractive to males. five traps were evaluated: pherocon 1 cp (wing trap), delta, "pvc 200", "pvc 250" and black round trap, where the wing trap was more effective in capturing males than the other traps tested. the wing trap was evaluated at 5, 30 and 60 cm. more males were caught at 30 cm above the ground level, while the height of 5 and 60 cm did not differ significantly.
Uso do ferom nio sexual sintético para captura de machos da tra a-das-crucíferas
MICHEREFF MIRIAN FERNANDES FURTADO,VILELA EVALDO FERREIRA,MICHEREFF FILHO MIGUEL,MAFRA-NETO AGENOR
Pesquisa Agropecuária Brasileira , 2000,
Abstract: Este trabalho teve como objetivo estudar a atra o dos machos da tra a-das-crucíferas (Plutella xylostella L., Lepidoptera: Yponomeutidae) por diferentes formula es do ferom nio sexual sintético. Os tratamentos consistiram em: 1) formula o comercial; 2) Z11,16:Ald; 3) Z11,16:Ac; 4) mistura 7:3 de 2+3; 5) mistura 5:5 de 2+3; 6) septos de borracha com hexano (testemunha); e 7) cinco fêmeas virgens. A formula o comercial do ferom nio sexual sintético propiciou maior captura de machos, n o diferindo significativamente de fêmeas virgens e da mistura 5:5 utilizadas como isca. Os componentes isoladamente foram pouco atrativos aos machos. Foram testados cinco modelos de armadilhas. A armadilha Pherocon 1 CP (33,2 machos/armadilha/noite) foi a mais eficiente na captura de machos, seguida pelas armadilhas Cilíndrica Aberta, Cilíndrica Fechada, Delta e Redonda Aberta. Três alturas de instala o da armadilha foram avaliadas. Ocorreu significativamente maior captura de machos na altura do ápice das plantas (30 cm do solo). As alturas de 5 e 60 cm n o diferiram entre si.
Aduba??o química, ataque do ácaro Aceria guerreronis e produtividade do coqueiro 'An?o-Verde'
Michereff Filho, Miguel;Sobral, Lafayette Franco;Ferreira, Joana Maria Santos;Rodrigues, Agna Rita dos Santos;Michereff, Mirian Fernandes Furtado;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008000300003
Abstract: the objective of this work was to determine the influence of nitrogen and potassium applied through fertigation, on green dwarf coconut productivity, and the intensity of aceria guerreronis keifer (acari: eriophyidae) attack. the study was carried out in a commercial orchard of eight-year old green dwarf coconut, in the neópolis county, sergipe state, brazil. the orchard received through microsprinkling (fertigation), since 2000, the following treatments (grams per plant per year of n and k): 135 and 135; 1,890 and 1,890; 810 and 135; 135 and 810; 1,890 and 810; and 810 and 1,890. four evaluations were done every three months between april, 2003 and march, 2004, on fruit bunches associated with the leaves 14 and 18. fruit production was influenced by n and k level applied on the coconut orchard, but mite infestation (from 8 to 80% of fruits) and the damage severity on fruits (from 3 to 47%) were not significantly affected by the chemical fertilization.
Initial studies of mating disruption of the tomato moth, Tuta absoluta (Lepidoptera: Gelechiidae) using synthetic sex pheromone
Michereff Filho, Miguel;Vilela, Evaldo F.;Jham, Gulab N.;Attygalle, Athula;Svatos, Ales;Meinwald, Jerrold;
Journal of the Brazilian Chemical Society , 2000, DOI: 10.1590/S0103-50532000000600011
Abstract: the potential of the synthetic major component of t. absoluta (meyrick) sex pheromone for mating disruption was studied in small plots (0.01 hectares) with fresh-market tomato crop. the effects of the application of the sex pheromone 3e,8z,11z-14:ac (from 0 to 80 g a.i./ha) were assessed on male orientation to pheromone baited traps, mating in cages and plant damage. the highest levels of interruption in male orientation (60-90%) were found in plots treated with 35 to 50 g/ha of sex pheromone. however, no treatment with pheromone was capable of significantly reducing the percentage of mined leaflets or bored fruits or the frequency of mating in cages compared to the control plots. the failure in mating disruption technique may be attributed to the composition of the synthetic pheromone, doses used, high pest population density, and mated female migration to the area treated.
Initial studies of mating disruption of the tomato moth, Tuta absoluta (Lepidoptera: Gelechiidae) using synthetic sex pheromone
Michereff Filho Miguel,Vilela Evaldo F.,Jham Gulab N.,Attygalle Athula
Journal of the Brazilian Chemical Society , 2000,
Abstract: The potential of the synthetic major component of T. absoluta (Meyrick) sex pheromone for mating disruption was studied in small plots (0.01 hectares) with fresh-market tomato crop. The effects of the application of the sex pheromone 3E,8Z,11Z-14:Ac (from 0 to 80 g a.i./ha) were assessed on male orientation to pheromone baited traps, mating in cages and plant damage. The highest levels of interruption in male orientation (60-90%) were found in plots treated with 35 to 50 g/ha of sex pheromone. However, no treatment with pheromone was capable of significantly reducing the percentage of mined leaflets or bored fruits or the frequency of mating in cages compared to the control plots. The failure in mating disruption technique may be attributed to the composition of the synthetic pheromone, doses used, high pest population density, and mated female migration to the area treated.
Efeito da aduba??o química na infesta??o do ácaro rajado e na produ??o do morangueiro
Ribeiro, Matheus Geraldo P de M;Michereff Filho, Miguel;Guedes, ítalo MR;Junqueira, Ana Maria R;Liz, Ronaldo S de;
Horticultura Brasileira , 2012, DOI: 10.1590/S0102-05362012000400018
Abstract: the effect of n:k relationship was evaluated when applied through fertigation, on two-spotted-spider mite, tetranychus urticae, infestation and on the yield of two strawberry cultivars. strawberry plants received weekly doses of n and k (g/plant) by drip irrigation: 0.27 and 0.13; 0.27 and 0.50; 0.27 and 0.76 and control (water+micronutrients). fruits were collected twenty two times during crop cycle. marketable, non-marketable fruits and other yield parameters were evaluated. leaf nutrient content analysis was performed to determine the nutritional condition of the plants. population density of t. urticae was evaluated weekly. a significant interaction between k doses and pest population was observed. higher doses of the nutrient reduced the number of eggs and active forms of the pest population and the cultivar diamante showed the smallest pest density. k and p showed negative correlation with both variables, but n showed a positive one. no effects were observed of fertilization on yield parameters due, probably, to the high mite infestation (>40 individuals per leaflet) in all treatments during most of the crop cycle. solely the use of different n:k ratios on the strawberry fertilization management at this specific experimental condition, was not sufficient to control two-spotted spider mite nor to reduce losses caused by that pest.
Resistance of Brazilian diamondback moth populations to insecticides
Oliveira, Alexandre Conte de;Siqueira, Herbert álvaro Abreu de;Oliveira, José Vargas de;Silva, Jefferson Elias da;Michereff Filho, Miguel;
Scientia Agricola , 2011, DOI: 10.1590/S0103-90162011000200004
Abstract: plutella xylostella is a recurring pest on cruciferous crops around the world. in brazil, it typically requires large number of insecticide sprays, which may lead to fast evolution of resistance. the aim of this study was to assess the susceptibility of brazilian diamondback moth populations to the insecticides abamectin, deltamethrin, and spinosad. leaf dip bioassays were used to determine mortality data obtained after 48 h of exposure to insecticides and subjected to probit analysis. the population from bonito, state of pernambuco, brazil, had the highest toxicity ratio (20.2 - fold) to abamectin compared to the reference population. the lc50 values for deltamethrin ranged from 85.2 to 360.1 mg l-1, demonstrating a high survival of populations in relation to this insecticide field dose rate (7.5 mg l-1). the toxicity ratios of the estimated lc50s, however, were very low (varying from 2.2 - to 4.2 - fold). most populations exhibited toxicity ratios for spinosad, ranging from 2.3 - to 5.1 - fold, while both the lc50 and lc95 values reflected a high susceptibility to the spinosad field dosage (120 mg l-1). only the bonito - pe population resisted to abamectin, while all p. xylostella populations were resistant to deltamethrin, but particularly susceptible to spinosad because of the absence of selection pressure with it in these areas.
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