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Search Results: 1 - 10 of 60992 matches for " MiaoYing Yu "
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Four recombinant pluripotency transcriptional factors containing a protein transduction domain maintained the in vitro pluripotency of chicken embryonic stem cells
MiaoYing Yu,Song Lian,HongBing Han,Kun Yu,GuiGuan Li,ZhengXing Lian,Ning Li
Science China Life Sciences , 2013, DOI: 10.1007/s11427-012-4426-4
Abstract: Long-term in vitro maintenance of embryonic stem cell (ESC) pluripotency enables the pluripotency and differentiation of ESCs in animals to be investigated. The ability to successfully maintain and differentiate chicken embryonic stem cells (cESCs) would provide a useful tool for avian biology research and would be a resource directly applicable to agricultural production. In this study, endogenous chicken pluripotency transcription factors, POUV, Sox-2, Nanog and Lin28 were cloned and expressed as recombinant proteins containing a nine consecutive arginine protein transduction domain (PTD). cESCs were cultured with these recombinant proteins to maintain cESC pluripotency in vitro. Cultured cESCs exhibited typical characteristics of pluripotency, even after six generations of rapid doubling, including positive staining for stage-specific embryonic antigen I, and strong staining for alkaline phosphatase. Expression levels of the pluripotency markers, POUV, Nanog, C-Myc, Sox-2 and Lin28 were the same as in uncultured stage X blastoderm cells, and most significantly, the formation of embryoid bodies (EBs) by 6th generation cESCs confirmed the ability of these cultured cESCs to differentiate into cells of all three embryonic germ layers. Thus, transcription factors could be translocated through the cell membrane into the intracellular space of cESCs by using a PTD of nine consecutive arginines and the pluripotency of cESCs could be maintained in vitro for at least six generations.
A two disulfide bridge Kazal domain from Phytophthora exhibits stable inhibitory activity against serine proteases of the subtilisin family
Miaoying Tian, Sophien Kamoun
BMC Biochemistry , 2005, DOI: 10.1186/1471-2091-6-15
Abstract: In this study, we predicted the inhibition constants of EPI1a and EPI1b to subtilisin A using the additivity-based sequence to reactivity algorithm (Laskowski algorithm). The atypical domain EPI1a, but not the typical domain EPI1b, was predicted to have strong inhibitory activity against subtilisin A. Inhibition assays and coimmunoprecipitation experiments showed that recombinant domain EPI1a exhibited stable inhibitory activity against subilisin A and was solely responsible for inhibition and interaction with tomato P69B subtilase.The finding that the two disulfide bridge atypical Kazal domain EPI1a is a stable inhibitor indicates that the missing two cysteines and their corresponding disulfide bond are not essential for inhibitor reactivity and stability. This report also suggests that the Laskowski algorithm originally developed and validated with typical Kazal domains might operate accurately for atypical Kazal domains.Proteases play essential roles in biological systems, not only digestion and protein turnover but also a diversity of specific processes [1]. To regulate the activity of proteases and avoid cellular damage, organisms also produce protease inhibitors [1]. So far, 48 distinct families of protease inhibitors have been described, one of which is the Kazal family of serine protease inhibitors (I1 family) [1]. Kazal type inhibitors are widely distributed in animals, apicomplexans and oomycetes. They are thought to play important roles in maintenance of normal cellular and physiological processes of animals [2,3], and pathogenesis of mammalian parasitic apicomplexans and plant pathogenic oomycetes [4-7]. Kazal-like serine protease inhibitors are defined by a conserved motif in their amino acid sequences. Typical Kazal domains contain six cysteine residues forming a 1–5/2–4/3–6 disulfide bond pattern [3,8]. Most Kazal domains described so far belong to this class. However, a novel class of Kazal domains has been described in recent years, in which the thi
Arabidopsis Actin-Depolymerizing Factor-4 Links Pathogen Perception, Defense Activation and Transcription to Cytoskeletal Dynamics
Katie Porter,Masaki Shimono,Miaoying Tian,Brad Day
PLOS Pathogens , 2012, DOI: 10.1371/journal.ppat.1003006
Abstract: The primary role of Actin-Depolymerizing Factors (ADFs) is to sever filamentous actin, generating pointed ends, which in turn are incorporated into newly formed filaments, thus supporting stochastic actin dynamics. Arabidopsis ADF4 was recently shown to be required for the activation of resistance in Arabidopsis following infection with the phytopathogenic bacterium Pseudomonas syringae pv. tomato DC3000 (Pst) expressing the effector protein AvrPphB. Herein, we demonstrate that the expression of RPS5, the cognate resistance protein of AvrPphB, was dramatically reduced in the adf4 mutant, suggesting a link between actin cytoskeletal dynamics and the transcriptional regulation of R-protein activation. By examining the PTI (PAMP Triggered Immunity) response in the adf4 mutant when challenged with Pst expressing AvrPphB, we observed a significant reduction in the expression of the PTI-specific target gene FRK1 (Flg22-Induced Receptor Kinase 1). These data are in agreement with recent observations demonstrating a requirement for RPS5 in PTI-signaling in the presence of AvrPphB. Furthermore, MAPK (Mitogen-Activated Protein Kinase)-signaling was significantly reduced in the adf4 mutant, while no such reduction was observed in the rps5-1 point mutation under similar conditions. Isoelectric focusing confirmed phosphorylation of ADF4 at serine-6, and additional in planta analyses of ADF4's role in immune signaling demonstrates that nuclear localization is phosphorylation independent, while localization to the actin cytoskeleton is linked to ADF4 phosphorylation. Taken together, these data suggest a novel role for ADF4 in controlling gene-for-gene resistance activation, as well as MAPK-signaling, via the coordinated regulation of actin cytoskeletal dynamics and R-gene transcription.
Expressed sequence tags from the oomycete fish pathogen Saprolegnia parasitica reveal putative virulence factors
Trudy Torto-Alalibo, Miaoying Tian, Kamal Gajendran, Mark E Waugh, Pieter van West, Sophien Kamoun
BMC Microbiology , 2005, DOI: 10.1186/1471-2180-5-46
Abstract: We generated 1510 expressed sequence tags (ESTs) from a mycelial cDNA library of S. parasitica. A total of 1279 consensus sequences corresponding to 525944 base pairs were assembled. About half of the unigenes showed similarities to known protein sequences or motifs. The S. parasitica sequences tended to be relatively divergent from Phytophthora sequences. Based on the sequence alignments of 18 conserved proteins, the average amino acid identity between S. parasitica and three Phytophthora species was 77% compared to 93% within Phytophthora. Several S. parasitica cDNAs, such as those with similarity to fungal type I cellulose binding domain proteins, PAN/Apple module proteins, glycosyl hydrolases, proteases, as well as serine and cysteine protease inhibitors, were predicted to encode secreted proteins that could function in virulence. Some of these cDNAs were more similar to fungal proteins than to other eukaryotic proteins confirming that oomycetes and fungi share some virulence components despite their evolutionary distanceWe provide a first glimpse into the gene content of S. parasitica, a reemerging oomycete fish pathogen. These resources will greatly accelerate research on this important pathogen. The data is available online through the Oomycete Genomics Database [1].Water molds such as Saprolegnia and Aphanomyces species are responsible for devastating infections on fish in aquaculture, fish farms and hobby fish tanks [2,3]. Members of the genus Saprolegnia cause saprolegniosis, a disease that is characterized by visible white or grey patches of filamentous mycelium on the body or fins of freshwater fish [2]. The oomycete Saprolegnia parasitica is economically one of the most important fish pathogens, especially on salmon and trout species. It causes tens of million dollar losses to aquaculture business worldwide, notably in Scotland, Scandinavia, Chile, Japan, Canada, and the USA [4,5]. S. parasitica infections are second only to bacterial diseases. In Japan
Isolation and Identification of soil bacteria containing ACC deaminase

Wei Jun,Pan Qilin,Zhang Yongqing,Qiu Bingsheng,Cat Miaoying,

生物多样性 , 1994,
Abstract: Some strains of soil bacteria were isolated by their ability to grow on ACC as a sole nitrogen source.ACC deaminase activity of these strains were determined by growth assaying,confirmed by chromatograthically identifying to decrease of ACC in the medium.Then systematic identification was done.
Thermophilic Bateria

Wei Jun,Cai Miaoying Institute of Microbiology,Academia Sinica,Beijing,

生物多样性 , 1993,
Abstract: Thermophiles have been of interest for a long time both to scientists and to the general public, and are believed to have a great potential in biotechnology and some products are already on the markets. For present the current status of research, here we collect a great deal of new information on the thermophilic (eu)bacteria, discuss the main properties and differences of thermophilic bacteria and archaea, The diveristy, ecology, and evolution of thermophiles are also briefly reviewed.
Cigarette Smoking Exacerbates the Adverse Effects of Age and Metabolic Syndrome on Subclinical Atherosclerosis: The Bogalusa Heart Study
Shengxu Li, Miaoying Yun, Camilo Fernandez, Jihua Xu, Sathanur R. Srinivasan, Wei Chen, Gerald S. Berenson
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0096368
Abstract: Age and metabolic syndrome are major risk factors for atherosclerosis. However, limited information is available regarding whether cigarette smoking, another major, modifiable risk factor, has synergistic effects with age and metabolic syndrome on subclinical atherosclerosis, particularly in young adults. This aspect was examined in 1,051 adults (747 whites and 304 blacks; aged 24–43 years) from the Bogalusa Heart Study. General linear models were used to examine the effects of cigarette smoking and its interactive effects with age and metabolic syndrome on carotid intima-media thickness (CIMT). After adjusting for age, race, and sex, current smokers had lower BMI (mean±SE: 27.4±0.4, 29.3±0.5, and 29.9±0.3 kg/m2 in current, former, and never smokers, respectively; p<0.0001) and lower levels of fasting glucose (82.8±0.9, 89.5±2.3, and 87.1±1.1 mg/dL, respectively; p = 0.001) and insulin (10.6±0.4, 14.2±1.0, 13.6±0. 6 μU/ml, respectively; p<0.0001). Despite being lean and having favorable levels of glucose and insulin, current smokers had greater CIMT (0.850±0.012, 0.808±0.011, and 0.801±0.006 mm, respectively; p = 0.0004). Importantly, cigarette smoking showed significant interactions with age and metabolic syndrome on CIMT: Age-related change in CIMT in current smokers was significantly greater (0.013±0.002 mm/year) than in nonsmokers (former and never smokers combined) (0.008±0.001 mm/year) (p for interaction = 0.005); the difference in CIMT between those with and without metabolic syndrome was significantly greater in current smokers (0.154±0.030 mm, p<0.0001) than in nonsmokers (0.031±0.014 mm, p = 0.03) (p for interaction<0.0001). In conclusion, cigarette smoking significantly exacerbates the adverse effects of age and metabolic syndrome on subclinical atherosclerosis in young adults, which underscores the importance of prevention and cessation of cigarette smoking behavior in the young.
Resonance as a probe of the electron superconducting gap in BaFe1.9Ni0.1As2
Jun Zhao,Louis-Pierre Regnault,Chenglin Zhang,Miaoying Wang,Zhengcai Li,Fang Zhou,Zhongxian Zhao,Pengcheng Dai
Physics , 2009, DOI: 10.1103/PhysRevB.81.180505
Abstract: The discovery of high-transition temperature (high-Tc) superconductivity near antiferromagnetism in iron arsenides raised the possibility of an unconventional superconducting mechansim1-8. The observation of clear Fermi surfaces and nodeless superconducting gaps by angle resolved photoemission9-12 suggests that electron pairing in these materials may be mediated by quasiparticle excitations between sign reversed hole and electron Fermi pockets5-8. Although the presence of a 'resonance' in the spin excitation spectrum found by inelastic neutron scattering13-17 is consistent with this picture18-20, there has been no direct evidence connecting the resonance to the superconducting gap energy. Here we show that for the optimally electron doped BaFe1.9Ni0.1As2 (Tc =20 K, Fig. 1c) iron arsenide superconductor, application of a magnetic field that suppresses the superconductivity and superconducting gap energy also reduces the intensity and energy of the resonance. These results suggest that the energy of the resonance is proportional to the electron pairing energy, and thus indicate that spin fluctuations are intimately related to the mechanism of superconductivity in iron arsenides.
Anisotropic Neutron Spin Resonance in Superconducting BaFe$_{1.9}$Ni$_{0.1}$As$_2$
O. J. Lipscombe,Leland W. Harriger,P. G. Freeman,M. Enderle,Chenglin Zhang,Miaoying Wang,Takeshi Egami,Jiangping Hu,Tao Xiang,M. R. Norman,Pengcheng Dai
Physics , 2010, DOI: 10.1103/PhysRevB.82.064515
Abstract: We use polarized inelastic neutron scattering to show that the neutron spin resonance below $T_c$ in superconducting BaFe$_{1.9}$Ni$_{0.1}$As$_2$ ($T_c=20$ K) is purely magnetic in origin. Our analysis further reveals that the resonance peak near 7~meV only occurs for the planar response. This challenges the common perception that the spin resonance in the pnictides is an isotropic triplet excited state of the singlet Cooper pairs, as our results imply that only the $S_{001}=\pm1$ components of the triplet are involved.
On the road: Clinical trials with stem cell extended to non-hematologic disease  [PDF]
Jinyang Yu, Yanqiu Yu
Advances in Bioscience and Biotechnology (ABB) , 2013, DOI: 10.4236/abb.2013.42031
Increasing evidence in scientific journals declares that stem cell can be used in human medicine for therapeutic purposes. We reviewed the lated literature on clinical trials conducted with stem cells. The main information was offered by
http://www.ClinicalTrials.gov. These clinical trials cover almost all human diseases, from hematologic diseases to non-hematologic diseases including Interventional trials and observational trials. In conclusion, at present, the clinical trials with stem cells have been extending to almost all human diseases. Optimal medicinal effect reported in some non-hematologic diseases is pushing the advance of stem cells therapy
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