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Search Results: 1 - 10 of 24755 matches for " Mi-Hyun Kim "
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Adolescents’ Estimation of Energy Content of Standard Portion Size of Foods and Its Association with Body Mass Index  [PDF]
Mi-Kyeong Choi, Min-Jung Ko, Mi-Hyun Kim
Food and Nutrition Sciences (FNS) , 2012, DOI: 10.4236/fns.2012.310177
Abstract: The purpose of this study is to identify the adolescents’ knowledge of the energy content of the standard portion size of foods and to investigate the association between their knowledge and energy intake and also body mass index (BMI). A total of 251 middle school adolescents participated in this study. Participants’ knowledge was assessed based on their estimation of the energy content of the standard portion size of foods. To estimate the energy intake of the subjects, 24-hr recall was used. The percentage of participants who accurately estimated (that is within 20% of the true value) the energy content of the standard portion size was calculated for each of the 32 typical foods. The food for which the most participants revealed the accurate estimation was cooked rice (39.5%). The proportion of students who overestimated the energy contents was highest for vegetables (98%), and oils and sugar (90%). The female students were more likely than males to provide the accurate estimation of energy contents for standard portion size of foods. After adjusting for age and sex, the estimation level ([estimation value/true value] × 100) of the energy content of some foods had a significant positive relationship with BMI, but had no significant relationship with reported energy intake. From these results, we concluded that the knowledge of energy content of food was poor among middle-school adolescents, with some gender difference, and that their estimation of the calorie contents of foods increased along with their BMI.
Insulin Receptor-Overexpressing β-Cells Ameliorate Hyperglycemia in Diabetic Rats through Wnt Signaling Activation
Mi-Hyun Kim, Seung-Hyun Hong, Moon-Kyu Lee
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0067802
Abstract: To investigate the therapeutic efficacy and mechanism of β-cells with insulin receptor (IR) overexpression on diabetes mellitus (DM), rat insulinoma (INS-1) cells were engineered to stably express human insulin receptor (INS-IR cells), and subsequently transplanted into streptozotocin- induced diabetic rats. Compared with INS-1 cells, INS-IR cells showed improved β-cell function, including the increase in glucose utilization, calcium mobilization, and insulin secretion, and exhibited a higher rate of cell proliferation, and maintained lower levels of blood glucose in diabetic rats. These results were attributed to the increase of β-catenin/PPARγ complex bindings to peroxisome proliferator response elements in rat glucokinase (GK) promoter and the prolongation of S-phase of cell cycle by cyclin D1. These events resulted from more rapid and higher phosphorylation levels of insulin-signaling intermediates, including insulin receptor substrate (IRS)-1/IRS-2/phosphotylinositol 3 kinase/v-akt murine thymoma viral oncogene homolog (AKT) 1, and the consequent enhancement of β-catenin nuclear translocation and Wnt responsive genes including GK and cyclin D1. Indeed, the higher functionality and proliferation shown in INS-IR cells were offset by β-catenin, cyclin D1, GK, AKT1, and IRS-2 gene depletion. In addition, the promotion of cell proliferation and insulin secretion by Wnt signaling activation was shown by 100 nM insulin treatment, and to a similar degree, was shown in INS-IR cells. In this regard, this study suggests that transferring INS-IR cells into diabetic animals is an effective and feasible DM treatment. Accordingly, the method might be a promising alternative strategy for treatment of DM given the adverse effects of insulin among patients, including the increased risk of modest weight gain and hypoglycemia. Additionally, this study demonstrates that the novel mechanism of cross-talk between insulin and Wnt signaling plays a primary role in the higher therapeutic efficacy of IR-overexpressing β-cells.
Effect of Conductivity of the Aqueous Solution on the Size of Printable Nanoparticle
Mi-Hyun Oh,Nam-Soo Kim,Sun-Mee Kang
Journal of Nanotechnology , 2012, DOI: 10.1155/2012/467812
Abstract: Direct writing technology using nano/microsize particles in aqueous solution is currently one of the leading candidates to bring a substantial advancement to the technical arena. However, little is known about an effect of conductivity of the solution including metal ions on nanoparticle size for the direct writing technology. It is believed that conductivity of solution can influence the size of particles in reducing environmental of aqueous solutions. In this study parameters which affect electric conductivity in solution were characterized by changing concentration of copper ion, concentration of surfactants, and anion of metal compounds. The mobility of ion in aqueous media with respect to copper ion concentration was the most pronounced factor to control the size of created copper nanometals in water. However, due to the high reactivity on large surface area, the nanocopper metal was oxidized in water. The electric conductivity varied in the range of 7 to 360?mS/cm when Cu(NO3)2 ? 3H2O dissolved in water from 0.03–3.0?mol/ d m 3 . In this condition, the size of nano particles can vary from 10 to 500?nm. Various concentrations of surfactants and two anion Cu compounds used to vary the conductivity of the solution to verify the effect of electric conductivity of solution on the particle size. Decreasing the conductivity had a corresponding effect on the particle size. The electric conductivity was decreased from 67 to 56?mS/cm by adding surfactant from 0.1 to 0.5?mol/ d m 3 consequently, the particle sizes were decreased from 89 to 21?nm. Copper nitrate and copper chloride were used to verify the anion effect on electrical conductivity and particle sizes. This effect was not dependent on the kind of ions chosen to change the conductivity. However, when Cu(NO3)2 ? 3H2O was used, the size of the particles was 89 nm, while it was 91?nm when CuCl2 ? 2H2O was used. 1. Introduction The characteristics of particles change as the particle size decreases to nanosize. This change can be important if it is used to improve product characteristics and can be applied in numerous areas. The nanoparticles have been adapted to produce printable devices, flexible antennae, display, and circuit board in industries. To improve conductivity and increase the contact area, various shapes such as octahedral Au nanoparticle [1], and Ag cubic [2] were made in condensed surfactant phase. The spherical, cylindrical, and web-shaped Cu and CuO were successfully synthesized by altering concentration of surfactants and pH of solutions in alcoholic media [3]. Additional tests were
Quantitative and Qualitative Analyses of the Cell Death Process in Candida albicans Treated by Antifungal Agents
Kyung Sook Kim,Young-Sun Kim,Ihn Han,Mi-Hyun Kim,Min Hyung Jung,Hun-Kuk Park
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0028176
Abstract: The death process of Candida albicans was investigated after treatment with the antifungal agents flucytosine and amphotericin B by assessing morphological and biophysical properties associated with cell death. C. albicans was treated varying time periods (from 6 to 48 hours) and examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM). SEM and AFM images clearly showed changes in morphology and biophysical properties. After drug treatment, the membrane of C. albicans was perforated, deformed, and shrunken. Compared to the control, C. albicans treated with flucytosine was softer and initially showed a greater adhesive force. Conversely, C. albicans treated with amphotericin B was harder and had a lower adhesive force. In both cases, the surface roughness increased as the treatment time increased. The relationships between morphological changes and the drugs were observed by AFM clearly; the surface of C. albicans treated with flucytosine underwent membrane collapse, expansion of holes, and shrinkage, while the membranes of cells treated with amphotericin B peeled off. According to these observations, the death process of C. albicans was divided into 4 phases, CDP0, CDP1, CDP2, and CDP4, which were determined based on morphological changes. Our results could be employed to further investigate the antifungal activity of compounds derived from natural sources.
Quantitative and Qualitative Analysis of the Antifungal Activity of Allicin Alone and in Combination with Antifungal Drugs
Young-Sun Kim, Kyung Sook Kim, Ihn Han, Mi-Hyun Kim, Min Hyung Jung, Hun-Kuk Park
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0038242
Abstract: The antifungal activity of allicin and its synergistic effects with the antifungal agents flucytosine and amphotericin B (AmB) were investigated in Candida albicans (C. albicans). C. albicans was treated with different conditions of compounds alone and in combination (allicin, AmB, flucytosine, allicin + AmB, allicin + flucytosine, allicin + AmB + flucytosine). After a 24-hour treatment, cells were examined by scanning electron microscopy (SEM) and atomic force microscopy (AFM) to measure morphological and biophysical properties associated with cell death. The clearing assay was conducted to confirm the effects of allicin. The viability of C. albicans treated by allicin alone or with one antifungal drug (AmB, flucytosine) in addition was more than 40% after a 24-hr treatment, but the viability of groups treated with combinations of more than two drugs was less than 32%. When the cells were treated with allicin alone or one type of drug, the morphology of the cells did not change noticeably, but when cells were treated with combinations of drugs, there were noticeable morphological changes. In particular, cells treated with allicin + AmB had significant membrane damage (burst or collapsed membranes). Classification of cells according to their cell death phase (CDP) allowed us to determine the relationship between cell viability and treatment conditions in detail. The adhesive force was decreased by the treatment in all groups compare to the control. Cells treated with AmB + allicin had a greater adhesive force than cells treated with AmB alone because of the secretion of molecules due to collapsed membranes. All cells treated with allicin or drugs were softer than the control cells. These results suggest that allicin can reduce MIC of AmB while keeping the same efficacy.
Conditional Deletion of Pten Leads to Defects in Nerve Innervation and Neuronal Survival in Inner Ear Development
Hyung Jin Kim, Hae-Mi Woo, Jihee Ryu, Jinwoong Bok, Jin Woo Kim, Sang Back Choi, Mi-Hyun Park, Hyun-Young Park, Soo Kyung Koo
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0055609
Abstract: All cellular phenomena and developmental events, including inner ear development, are modulated through harmonized signaling networks. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN), a tumor suppressor, is a major signaling component involved in cross talk with key regulators of development; i.e., Wnt, Notch, and bone morphogenetic proteins. Although Pten function has been studied in various systems, its role in inner ear development is poorly understood. Here, we used inner ear-specific Pten conditional knockout mice and examined the characteristics of the inner ear. In a detailed analysis of the phenotype, reduced cochlear turning and widened epithelia were observed. Phalloidin staining of sensory epithelium revealed that hair cell patterns were disturbed; i.e., additional rows of hair cells were discovered. The neural abnormality revealed a reduction in and disorganization of nerve fibers, including apoptosis at the neural precursor stage. Pten deficiency induced increased phosphorylation of Akt at Ser473. The elevation of inhibitory glycogen synthase kinase 3β Ser9 phosphorylation (pGSK3β) was sustained until the neuronal differentiation stage at embryonic day 14.5, instead of pGSK3β downregulation. This is the first report on the influence of Pten/Akt/GSK3β signaling on the development of spiral ganglia. These results suggest that Pten is required for the maintenance of neuroblast number, neural precursors, and differentiation in the inner ear.
Patterns of Gene Expression Associated with Pten Deficiency in the Developing Inner Ear
Hyung Jin Kim, Jihee Ryu, Hae-Mi Woo, Samuel Sunghwan Cho, Min Kyung Sung, Sang Cheol Kim, Mi-Hyun Park, Taesung Park, Soo Kyung Koo
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0097544
Abstract: In inner ear development, phosphatase and tensin homolog (PTEN) is necessary for neuronal maintenance, such as neuronal survival and accurate nerve innervations of hair cells. We previously reported that Pten conditional knockout (cKO) mice exhibited disorganized fasciculus with neuronal apoptosis in spiral ganglion neurons (SGNs). To better understand the genes and signaling networks related to auditory neuron maintenance, we compared the profiles of differentially expressed genes (DEGs) using microarray analysis of the inner ear in E14.5 Pten cKO and wild-type mice. We identified 46 statistically significant transcripts using significance analysis of microarrays, with the false-discovery rate set at 0%. Among the DEGs, expression levels of candidate genes and expression domains were validated by quantitative real-time RT-PCR and in situ hybridization, respectively. Ingenuity pathway analysis using DEGs identified significant signaling networks associated with apoptosis, cellular movement, and axon guidance (i.e., secreted phosphoprotein 1 (Spp1)-mediated cellular movement and regulator of G-protein signaling 4 (Rgs4)-mediated axon guidance). This result was consistent with the phenotypic defects of SGNs in Pten cKO mice (e.g., neuronal apoptosis, abnormal migration, and irregular nerve fiber patterns of SGNs). From this study, we suggest two key regulatory signaling networks mediated by Spp1 and Rgs4, which may play potential roles in neuronal differentiation of developing auditory neurons.
The susceptibility of Anopheles lesteri to infection with Korean strain of Plasmodium vivax
Deepak Joshi, Wej Choochote, Mi-Hyun Park, Jung-Yeon Kim, Tong-Soo Kim, Wannapa Suwonkerd, Gi-Sik Min
Malaria Journal , 2009, DOI: 10.1186/1475-2875-8-42
Abstract: The comparative susceptibility of An. lesteri, An. sinensis and An. pullus was studied by feeding laboratory-reared mosquitoes on blood from patients carrying gametocytes from Korea and Thailand.In experimental feeding with Korean strain of P. vivax, oocysts developed in An. lesteri, An. sinensis and An. pullus. Salivary gland sporozoites were detected only in An. lesteri and An. sinensis but not in An. pullus. Large differences were found in the number of sporozoites in the salivary glands, with An. lesteri carrying much higher densities, up to 2,105 sporozoites in a single microscope field of 750 × 560 μM, whereas a maximum of 14 sporozoites were found in any individual salivary gland of An. sinensis. Similar results were obtained from a susceptibility test of two different strains of An. sinensis to Thai isolate of P. vivax, and differences in vector susceptibility according to geographical variation were not detected.The high sporozoite rate and sporozoite loads of An. lesteri indicate that this species is highly susceptible to infection with P. vivax. Anopheles sinensis appears to have a markedly reduced ability to develop salivary gland infection, whilst in An. pullus, no sporozoites were found in the salivary glands. Provided that the survival rate of An. lesteri is sufficiently high in the field, it would be a highly competent vector of vivax malaria.Plasmodium vivax malaria has re-emerged in many malaria-endemic areas, where the disease was believed to have been eradicated [1]. In South Korea, it was assumed that malaria had been eradicated, since no indigenous case was reported after 1978. However, following the detection of a case in 1993, the annual incidence of malaria increased dramatically and reached a peak of 4,142 cases in 2000 [2-5]. In the meantime, malaria cases in North Korea increased from 1,085 in 1998 to 296,540 in 2001 [6]. As a result, malaria has gained renewed attention as a public health problem throughout the Korean Peninsula.So far, e
Association of the programmed cell death 1 (PDCD1) gene polymorphism with ankylosing spondylitis in the Korean population
Sang-Hoon Lee, Yeon-Ah Lee, Doo-Hyun Woo, Ran Song, Eun-Kyung Park, Mi-Hyun Ryu, Young-Hoon Kim, Kyoung-Soo Kim, Seung-Jae Hong, Myung Yoo, Hyung-In Yang
Arthritis Research & Therapy , 2006, DOI: 10.1186/ar2071
Abstract: Ankylosing spondylitis (AS) is an important chronic inflammatory disease with an incidence range of between 0.5% and 1.0% [1,2]. Many genetic and environmental factors have been suggested to have some role in the development of AS [3]. However, the pathogenesis of AS is still unclear. Human leukocyte antigen (HLA) B27 makes up only a small proportion of the overall risk for spondyloarthritis. Fewer than 5% of HLA-B27-positive people in the general population develop these diseases [4]. In contrast, 20% of the HLA-B27-positive relatives of AS patients are affected. Family studies have suggested that HLA-B27 contributes to about 37% of the overall genetic risk for spondyloarthritis [5-7], which suggests the involvement of other genes in the development of AS. Another new susceptible gene therefore needs to be identified.Recently, the PDCD1 (programmed cell death 1) gene polymorphism was reported to be associated with systemic lupus erythematosus (SLE) [8,9], lupus nephritis [10,11] and seronegative rheumatoid arthritis (RA) [12]. It was also suggested that lupus nephritis and the seronegative RA susceptibility was associated with the PD-1.3 (position 7,146) A allele in a northern Sweden population.The PD-1 molecule is a negative regulator of T cells [13] that belongs to the immunoglobulin receptor superfamily. It encodes a 55 kDa type 1 transmembrane inhibitory immunoreceptor and is responsible for the negative regulation of T-cell activation and peripheral tolerance [14]. Nishimura and Honjo [15] reported that PD-1 expression was observed only in activated T and B cells as well as in the early lymphoid precursors. PD-1 is actively expressed on the cell surface during the activation of T and B cells. The cytoplasmic immunoreceptor tyrosine-based inhibitory motif of PD-1 was activated by an interaction between PD-1 and its corresponding ligands, PDL-1 (B7-H1) and PDL-2 (B7-DC) [15,16], which induces the inhibitory signal to inhibit the proliferation of T and B cells to
Titanium dioxide particle – induced goblet cell hyperplasia : association with mast cells and IL-13
Mi-Hyun Ahn, Chun-Mi Kang, Choon-Sik Park, Sang-Jun Park, Taiyoun Rhim, Pyeong-Oh Yoon, Hun Chang, Soo-Ho Kim, Hiroko Kyono, Kwang Kim
Respiratory Research , 2005, DOI: 10.1186/1465-9921-6-34
Abstract: To understand this, the numbers of goblet cells, Muc5ac (+) expressing epithelial cells and IL-13 expressing mast cells were measured in the trachea of sham or TiO2 particles – treated rats using periodic acid-Schiff, toluidine blue and immunohistochemical staining. RT-PCR for Muc-1, 2 and 5ac gene transcripts was done using RNA extracted from the trachea. Differential cell count and IL-13 levels were measured in bronchoalveolar lavage (BAL) fluid. In pretreatment groups, cyclophosphamide (CPA) or dexamethasone (DEX) was given before instillation of TiO2. TiO2 treatment markedly increased Muc5ac mRNA expression, and Muc5ac (+) or PAS (+) epithelial cells 48 h following treatment.The concentration of IL-13 in BAL fluids was higher in TiO2 treated – rats when compared to those in sham rats (p < 0.05). Pretreatment with cyclophosphamide (CPA) decreased the number of neutrophils and eosinophils in BAL fluid of TiO2 treated – rats (p < 0.05), but affected neither the percentage of PAS (+) cells, nor IL-13 levels in the BAL fluids (p > 0.05). In contrast, pretreatment with dexamethasone (DEX) diminished the percentage of PAS (+) cells and the levels of IL-13 (p < 0.05). TiO2 treatment increased the IL-13 (+) mast cells (p < 0.05) in the trachea, which was suppressed by DEX (p < 0.05), but not by CPA pretreatment (p > 0.05). In addition there were significant correlations of IL-13 (+) rate of mast cells in the trachea with IL-13 concentration in BAL fluid (p < 0.01) and with the percentage of Muc5ac (+) cells in the sham and TiO2 treated rats (p < 0.05).In conclusion, TiO2 instillation induces GCH and Muc5ac expression, and this process may be associated with increased production of IL-13 by mast cells.Excessive mucus secretion is one of the major clinical manifestations of chronic airway diseases such as asthma, chronic bronchitis, and cystic fibrosis [1]. The excessive mucus is attributed to goblet cell hyperplasia (GCH) and submucosal gland hypertrophy, which are hallma
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