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Search Results: 1 - 10 of 17754 matches for " Mi Lan Kang "
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Induction of Neuronal Differentiation of Rat Muscle-Derived Stem Cells in Vitro Using Basic Fibroblast Growth Factor and Ethosuximide
Mi Lan Kang,Jin Seon Kwon,Moon Suk Kim
International Journal of Molecular Sciences , 2013, DOI: 10.3390/ijms14046614
Abstract: Several studies have demonstrated that basic fibroblast growth factor (bFGF) can induce neural differentiation of mesenchymal stem cells. In this study, we investigated the neural differentiation of muscle-derived stem cells (MDSCs) following treatment with bFGF and ethosuximide, a small molecule used as an anticonvulsant in humans. Stem cells isolated from rat skeletal muscle (rMDSCs) were pre-induced by culturing with 25 ng/mL bFGF for 24 h and then were transferred to a medium supplemented with or without 4 mM ethosuximide. Neuronal differentiation was assessed by immunocytochemical and western blotting analyses of marker expression. Immunocytochemistry of rMDSCs treated with bFGF and ethosuximide identified abundant cells expressing neuronal markers (TuJ1, neuron-specific class III β-tubulin; NeuN, neuronal nuclear antigen; and NF-MH; neurofilament M and H). Olig2 (oligodendrocyte transcription factor 2)-positive cells were also observed, indicating the presence of oligodendrocyte lineage cells. These findings were substantiated by western blotting analysis of marker proteins. In particular, the expression of NeuN and TuJ1 was significantly higher in rMDSCs treated with ethosuximide and bFGF than in cells stimulated with bFGF alone (NeuN, p < 0.05 and TuJ1, p < 0.001). Expression of the astrocyte marker GFAP (glial fibrillary acidic protein) was not detected in this study. Collectively, the results showed that treatment with bFGF and ethosuximide induced effective transdifferentiation of rMDSCs into cells with a neural-like phenotype. Notably, rMDSCs treated with a combination of bFGF plus ethosuximide showed enhanced differentiation compared with cells treated with bFGF alone, implying that ethosuximide may stimulate neuronal differentiation.

LIANG Liang,JIN Mi-hua,WAN Yu-lan,YING Sheng-kang,

高分子学报 , 1992,
Abstract: In present paper, the properties of charge-mosaic membrane prepared from irradiated poly(styrene-butadiene-4-vinylpyridine) triblock copolymer were investigated. The results showed that the fluxes of water and electrolyte through the membrane are 2.40×10-5 mol/cm-3 min, and 2.10×10-3 mol/cm-2 sec respectively when the concentrations of KCl are 0.1 mol/1 and 10-3 mol/1 on the two sides of membrane. Experimental results of separation showed that charge-mosaic membrane has better dialysis properties for permeation to salt but not to high mole-cular weight or low molecular weight non-electrolytes.For mixed solution of KCl and protein,KCl and sucrose,the fluxes of KCl are 1.07×10-8 mol/cm2sec and 1.10×10-8 mol/cm2sec respectively.
Pluripotency of induced pluripotent stem cells
Lan Kang, Shaorong Gao
Journal of Animal Science and Biotechnology , 2012, DOI: 10.1186/2049-1891-3-5
Abstract: An iPS cell is induced from a non-pluripotent cell, but possesses pluripotency similar to that of ES cells. Takahashi and Yamanaka (2006) first achieved this landmark breakthrough by reprograming mouse embryonic fibroblasts (MEFs) into this new type of pluripotent stem cell via the ectopic expression of only four transcription factors, namely Oct4, Sox2, Klf4 and c-Myc. This new procedure circumvented the need for an oocyte, which is required by an earlier method of generating customized pluripotent stem cells termed somatic cell nuclear transfer (SCNT)-mediated nuclear reprogramming [1-3]. Since the discovery of iPS cells, the field has attracted a great amount of scientific and public attention because of the undefined mechanism by which the developmental potential of the cells is reverted and the potential for clinical applications using patient specific iPS cells. The generation of iPS cells from individual patients has raised the hope of treatments for numerous degenerative and genetic diseases [4-11].Unlike normal fertilization or the generation of SCNT-ES cells, the creation of iPS cells is a longer process that results in a heterogeneous mixture of cells with various developmental potentials. In the primary culture, iPS cells are usually present together with the original somatic cells, transformed cells and partially reprogrammed cells. Indeed, iPS cells are only approximately 0.1% to 1% of the total cells used for reprogramming. Moreover, only very small proportions of these cells are fully reprogrammed based on stringent criteria for evaluating pluripotency. Therefore, it is necessary to establish a molecular standard to distinguish fully reprogrammed iPS cells from those that are partially reprogrammed, especially for human iPS cells that may eventually be used for clinical applications.In the present review, we will summarize the most recent progress toward understanding the pluripotency of mouse iPS cells at the functional and molecular levels. We anti
Microscopic observation of the intercellular transport of CdTe quantum dot aggregates through tunneling-nanotubes  [PDF]
Lan Mi, Rongling Xiong, Yu Zhang, Weidong Yang, Ji-Yao Chen, Pei-Nan Wang
Journal of Biomaterials and Nanobiotechnology (JBNB) , 2011, DOI: 10.4236/jbnb.2011.22022
Abstract: Various inorganic nanoparticles are being considered for applications in life science as fluorescent labels and for such therapeutic applications as drug delivery or targeted cell destruction. It is of importance to understand their intercellular transport behaviors and mechanisms. Here, the intercellular transport of internalized CdTe quantum dot (QD) aggregates through tunneling-nanotubes (TNTs) between human hepatocellular carcinoma cells was studied by time-resolved confocal fluorescence microscopy. TNTs are known to connect eukaryotic cells to provide important pathways for intercellular communications. The formation, shrinkage, elongation and rupture of TNTs were clearly observed by microscopy. We found TNTs contained only F-actin or both microtubules and F-actin. Two transport modes for QD aggregates through the TNTs were observed: the microtubule-based bidirectional motion and the ac-tin-dependent unidirectional motion. The mean square displacement analyses revealed that the intercellular transportations of QDs along TNTs were mediated by active processes. The bidirectional intercellular transport of QDs within lysosomes through the TNT was also observed.
A Study on Tropical Land Cover Classification Using ALOS PALSAR 50 m Ortho-Rectified Mosaic Data  [PDF]
Lan Mi, Nguyen Thanh Hoan, Ryutaro Tateishi, Kotaro Iizuka, Bayan Alsaaideh, Toshiyuki Kobayashi
Advances in Remote Sensing (ARS) , 2014, DOI: 10.4236/ars.2014.33014
Abstract: The main objective of this study is to find better classifier of mapping tropical land covers using Synthetic Aperture Radar (SAR) imagery. The data used are Advanced Land Observing Satellite (ALOS) Phased Array type L-band Synthetic Aperture Radar (PALSAR) 50 m ortho-rectified mosaic data. Training data for forest, herbaceous, agriculture, urban and water body in the test area located in Kalimantan were collected. To achieve more accurate classification, a modified slope correction formula was created to calibrate the intensity distortions of SAR data. The accuracy of two classifiers called Sequential Minimal Optimization (SMO) and Random Forest (RF) were applied and compared in this study. We focused on object-based approach due to its capability of providing both spatial and spectral information. Optimal combination of features was selected from 32 sets of features based on layer value, texture and geometry. The overall accuracy of land cover classification using RF classifier and SMO classifier was 46.8% and 55.6% respectively, and that of forest and non-forest classification was 74.4% and 79.4% respectively. This indicates that RF classifier has better performance than SMO classifier.
The nestling diet of fairy pitta pitta nympha on Jeju Island, Korea  [PDF]
Eun-Mi Kim, Chan-Ryul Park, Chang-Wan Kang, Se-Jae Kim
Open Journal of Ecology (OJE) , 2012, DOI: 10.4236/oje.2012.24021
Abstract: The nestling diet of the Fairy Pitta (pitta nympha) was studied by videotaping during breeding period in Jeju Island, 2009. Earthworms of several species were the most common food resources for nestlings, averaging 82% of all items, followed by 4% of Homoptera larvae. The remaining was only rarely recorded, including Lepidopteran larvae and adults, slugs, spiders, beetle adults and larvae (Coleoptera) and grasshoppers. Adults provided the number of preys ranging from 1 to 7 items to chicks per one visit. The average value of prey number per visit was 3.0 (SD = 1.38). The estimated average length of prey was 5.7 cm (SD = 2.85), and 96% of the food items were smaller than 10 cm. The staying time for feeding in an early stage was longer than other stages. Provision rate at a forenoon (mean ± SD, 14.7 ± 4.92) and an afternoon time (15.8 ± 5.30) was not significantly higher than that of noon time (11.7 ± 4.49). These results provide basic information for conservation action of international endangered species of this species.
Measurement and numerical simulation of the bandgap in hollow-core photonic crystal fibers

Mi Yan,Hou Lan-Tian,Zhou Gui-Yao,Wang Kang,Chen Chao,Gao Fei,Liu Bo-Wen,Hu Ming-Lie,

物理学报 , 2008,
Abstract: The full-vector plane-wave method was used to calculate the bandgaps of the photonic crystal fiber with triangular structure. The transmission spectrum of the hollow-core photonic crystal fiber fabricated by Yanshan University was measured, and we obtained the relation between the transmission intensity and the wavelength in visible region. In the subsequent experiment, the mode field patterns were observed. The theoretical simulation is well consistent with the experimental result.
Comprehensive understanding of size-, shape-, and composition-dependent polarizabilities of SimCn (m, n = 1-4) clusters
You-Zhao Lan,Hong-Lan Kang,Tao Niu
Physics , 2011,
Abstract: We theoretically investigate the size-, shape-, and composition-dependent polarizabilities of the SimCn (m, n = 1 - 4) clusters by using the density functional based coupled perturbed Hartree-Fock method. The size-dependence of the polarizabilities of the SimCn (m, n = 1 - 4) clusters is more complicated than that of pure Sim and Cn (m, n = 1 - 8) clusters because for a given cluster size the heteroatomic clusters have more isomers than the homoatomic ones. For the shape-dependence, we consider three kinds of shape, linear (chain), prolate, and compact. For most clusters, we can clearly observe orders of {\alpha}(linear) > {\alpha}(prolate) and {\alpha}(prolate) > {\alpha}(compact) for a given composition. The composition-dependence of polarizabilities reveals that the linear clusters have an obvious larger polarizability than both the prolate and the compact clusters especially for a given m/n value. The shape effect makes a main contribution to determine the size of the polarizability. To understand the size of polarizability and the evolution of polarizability, we have tried many factors, such as the energy gap and binding energy, and defined a new parameter ({\Delta}q) that characterizes the redistribution of charge in cluster. We find that both the binding energy and the {\Delta}q are more available than the energy gap for reflecting the evolution of polarizabilities provided that both the cluster shape and one of the components in cluster are fixed. The correlation between the polarizability and the energy gap is poor, in agreement with the previous results.
Provincial Technology and Productivity Gaps in China Using Meta-Frontier  [PDF]
Kang Sang-Mok, Kim Moon-Hwee, Piao Hui-Lan
Modern Economy (ME) , 2013, DOI: 10.4236/me.2013.48059

This study intends to analyze not only technical efficiency and technology gap but also productivity change and productivity gap in thirty one provinces in three regions of China for 1995 through 2008 adapting meta-frontier model. Also, we decompose the productivity into technical progress change and technical efficiency change in order to check a main component to the changes. We found that the group technical efficiency (or productivity) and meta-technical efficiency (or productivity) of provinces are different from each other. With respect to the meta-frontier, the eastern region shows the highest levels in terms of productivity as well as technical efficiency. Regarding the meta-productivity change, the productivity declines in the central and western regions have been mainly led by the drop on the efficiency change while the productivity growth in the eastern region has been driven by the technical progress. Lastly, by applying the meta-frontier, we found a very important fact that there exists a difference between the group-frontiers and the meta-frontiers, which implies that we may give wrong information and distort a reality with the group-frontier when measuring the technical efficiencies or productivity changes.

Semistable Higgs bundles and representations of algebraic fundamental groups: Positive characteristic case
Guitang Lan,Mao Sheng,Kang Zuo
Mathematics , 2012,
Abstract: Let $k$ be an algebraic closure of finite fields with odd characteristic $p$ and a smooth projective scheme $\mathbf{X}/W(k)$. Let $\mathbf{X}^0$ be its generic fiber and $X$ the closed fiber. For $\mathbf{X}^0$ a curve Faltings conjectured that semistable Higgs bundles of slope zero over $\mathbf{X}^0_{\mathbb{C}_p}$ correspond to genuine representations of the algebraic fundamental group of $\mathbf{X}^0_{\mathbb{C}_p}$ in his $p$-adic Simpson correspondence. This paper intends to study the conjecture in the characteristic $p$ setting. Among other results, we show that isomorphism classes of rank two semistable Higgs bundles with trivial chern classes over $X$ are associated to isomorphism classes of two dimensional genuine representations of $\mathbf{X}^0$ and the image of the association contains all irreducible crystalline representations. We introduce intermediate notions strongly semistable Higgs bundles and quasi-periodic Higgs bundles between semistable Higgs bundles and representations of algebraic fundamental groups. We show that quasi-periodic Higgs bundles give rise to genuine representations and strongly Higgs semistable are equivalent to quasi-periodic. We conjecture that a Higgs semistable bundle is indeed strongly Higgs semistable.
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