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Search Results: 1 - 10 of 462665 matches for " Menegucci Edna A. "
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Sensibilidade toxicológica e especificidade do teste de microfixa??o de complemento na detec??o de toxinas botulínicas C e D em meio de cultura e fígado de camundongos
Menegucci, Edna A.;Dutra, Iveraldo S.;D?bereiner, Jürgen;
Pesquisa Veterinária Brasileira , 1998, DOI: 10.1590/S0100-736X1998000200001
Abstract: the toxicological sensitivity and specificity of the micro-complement fixation test (mcf) for the detection of botulinum toxins c and d were studied in supernatants of the bacterial cultures and in livers of mice inoculated with lethal and sublethal doses. botulinum toxins c and d were produced in hemoline culture medium, titered through the determination of ld50 by the mouse test and adjusted to dilutions of 10, 1, 0.1, 0.01 and 0.001 ld50. two experimental models were used to determine the toxicological sensitivity of mcf in the supernatant of the culture medium with the dilutions described, and also in liver extracts of mice weighing 20 g and inoculated with the same dilutions. detection of the botulinum toxins was attempted in liver extracts of mice which had received lethal doses of the respective toxins, and in others which had been inoculated with sublethal doses and were sacrificed in intervals of 5 days. the results show that the toxicological sensitivity of mcf, regarding the two types of toxins at the level of 0.001 ld50, was 100% when the supernatants of the culture medium were tested; this means that the sensitivity was 100 times higher than with the mouse test. the toxicological sensitivity of mcf in the liver extracts of mice inoculated with 1 and 10 ld50 of botulinum toxins c and d was inferior, giving values of 100, 80, 89 and 72% respectively. by this test it was also possible to detect botulinum toxins type c and d in liver extracts of mice inoculated with sublethal doses, up to 15 days after the injection. the specificity of mcf was 88% and 92%, when liver extracts of healthy control mice were tested and when challenged with antitoxins c and d; and 100% when challenged with the supernatant of the culture medium. these results indicate that mcf could be of importance for research and could substitute in vivo tests.
Sensibilidade toxicológica e especificidade do teste de microfixa o de complemento na detec o de toxinas botulínicas C e D em meio de cultura e fígado de camundongos
Menegucci Edna A.,Dutra Iveraldo S.,D?bereiner Jürgen
Pesquisa Veterinária Brasileira , 1998,
Abstract: No presente estudo pretendeu-se verificar a sensibilidade toxicológica e especificidade do Teste de Microfixa o de Complemento (MCF) na detec o de toxinas botulínicas C e D no sobrenadante de cultivos bacterianos e em fígados de camundongos inoculados com doses letais e subletais. As toxinas foram produzidas em meio de cultura Hemoline, tituladas através da determina o da DL50 pelo Bioensaio em Camundongo e diluídas nas concentra es de 10, 1, 0,1, 0,01 e 0,001 DL50. Desta forma, foram utilizadas em dois modelos experimentais, onde foi determinada a sensibilidade toxicológica do MCF no sobrenadante do meio de cultura com as dilui es descritas acima e ainda em extratos hepáticos de camundongos com peso corporal de 20g, inoculados com as mesmas dilui es. A tentativa de evidencia o das toxinas botulínicas nos extratos hepáticos de camundongos foi realizada através da sua extra o após a morte pela administra o das doses letais e ainda pelo sacrifício dos animais inoculados com doses subletais, em intervalos de 5 dias. Os resultados evidenciaram uma sensibilidade toxicológica para o MCF de 100% para os dois tipos de toxinas ao nível de 0,01 DL50, quando testados os sobrenadantes de meio de cultura, portanto 100 vezes superior ao Bioensaio em Camundongo. A sensibilidade toxicológica do MCF, quando examinados extratos hepáticos de camundongos inoculados com 1 e 10 DL50 de toxinas botulínicas C e D, foi inferior, com valores de 100, 80, 89 e 72%, respectivamente. Pelo teste foi possível detectar toxinas botulínicas tipos C e D nos extratos hepáticos de camundongos inoculados com doses subletais até 15 dias após a sua inocula o. A especificidade do MCF foi de 88 e 92%, quando testados extratos hepáticos de camundongos sadios, e confrontados com as antitoxinas C e D; e 100% no sobrenadante do meio de cultura. Os resultados apontam para uma possível utiliza o do teste como importante instrumento de pesquisa e ainda na eventual substitui o dos testes in vivo pelas suas implica es éticas e limita es práticas.
HISTOPATHOLOGY OF THE TELENCEPHALON AND DIENCEPHALON OF T1LAPIA NILOTICA EXPOSED TO SUBLETHAL DOSE OF MALATHION S-[1,2-DI-(ETHOXYCARBONYL ETHYL) DIMETHYL PHOSPHOROTHIOLOTHIONATE]
EDNA A. AMPARADO
BIOTROPIA : the Southeast Asian Journal of Tropical Biology , 1992,
Abstract: A 35-day exposure of Tilapia nilotica embryos to sublethal doses of 3.0 ppm and 0.3 ppm malathion, S-[l,2-di-(ethoxycarbonyl ethyl) dimethyl phosphorothiolothionate], commercial grade, EC 57, produces cellular and ultrastructure changes in the brain. A number of nuclear centers of the treated animals are markedly larger than those of the control. Aberrant features observed in day-45 embryos are the neoplastic masses and increased vascularization. Ultrastructure defects include the presence of nuclear blebs, cytoplasmic vacuolations and increased lysosomal bodies.
Evolución histórica de los rendimientos educativos en México: 1987-2004
Villarreal Peralta, Edna María;
Estudios sociales (Hermosillo, Son.) , 2008,
Abstract: this paper examines the impact of educational level on income wages in mexico, for the period 1987-2004. for this reason, we assess the historical evolution of educational returns using data from the national survey of urban employment (eneu). also, we estimate three alternative models: first, taking years of study as a major explanatory variable; secondly, incorporating variable dummies for kinship and marital status; and, thirdly, considering educational levels. the results obtained show that the highest marginal returns correspond to individuals with higher educational levels. on the other hand, when getting the yields by gender, it seems that those of women surpass men's. finally, a sharp decline in returns during periods of economic crisis, can be observed.
From Riva Rocci, Recklinghausen and Korotkoff to nowadays: the challenge of blood pressure measurement accuracy
Edna A.M. Arcuri
Online Brazilian Journal of Nursing , 2005,
Abstract: This review points out the centenary of the Korotkoff Sounds and the importance of his study to blood pressure control and the development of the cardiovascular area. The author aims to homage the Russian investigator and other scientists who developed the sphygmomanometer studies in the previous centuries. It presents an overview of the literature related to cuff size, from Recklinghausen’s study up to the last American Heart Association recommendation for cuff size published in 2005. Cuff width influence on blood pressure measurement and its consequences in the misdiagnosis and mistreatment of hypertension is discussed. It emphasizes particularly the imprecision of blood pressure measurements in lean and normal weighted persons, as well as the lack of attention of the AHA to the results reached by scientific groups dedicated to the specific area.
Evolución histórica de los rendimientos educativos en México: 1987-2004
Edna María Villarreal Peralta
Estudios sociales (Hermosillo, Son.) , 2008,
Abstract: En este trabajo se analiza el impacto del nivel educativo sobre el ingreso salarial en México para el periodo 1987-2004. Para ello, se calcula la evolución de los rendimientos educativos empleando datos de la Encuesta Nacional de Empleo Urbano (ENEU). Se estiman tres modelos alternativos: primero, se toman a os de estudio como variable explicativa principal; segundo, se incorporan variables dummies para estado civil y parentesco y, tercero, se consideran niveles educativos. Se obtiene que los mayores rendimientos marginales corresponden a los individuos con nivel educativo más elevado. Asimismo, al estimar los rendimientos por género, se encuentra que los de las mujeres superan a los de los hombres. Por último, aparece una marcada disminución en los rendimientos en los periodos de crisis económica.
Rede ONSA e o Projeto Genoma Humano do Cancer: Contribui??o ao Genoma Humano
Kimura, Edna T.;Baía, Gilson S.;
Arquivos Brasileiros de Endocrinologia & Metabologia , 2002, DOI: 10.1590/S0004-27302002000400003
Abstract: the human cancer genome project - hcgp - has been the most important contribution of brazilian science to the understanding of the human genome. this project was co-sponsored by fapesp (s?o paulo research foundation) and the ludwig institute for cancer research. twenty-nine sequencing laboratories integrated by a bioinformatic service and a laboratory carried out the project. this effort has generated more than 1 million of expressed sequence tags (ests) in various human cancers. most of these sequences are available to public accessing at ncbi gene bank (www.ncbi.nlm.nih.gov by orestes keyword). currently there are several ongoing projects that utilize the information and genomic library generated by hcgp. these studies are focused in the characterization of a complete physical map of genes, differential gene expression in tumors and also in the structural and functional understanding of the expressed gene. in the future, the generated knowledge would contribute to new insights into the prevention, diagnosis and therapeutic procedures in cancer and other diseases.
O sujeito da neurociência: da naturaliza??o do homem ao re-encantamento da natureza
Russo, Jane A.;Ponciano, Edna L. T.;
Physis: Revista de Saúde Coletiva , 2002, DOI: 10.1590/S0103-73312002000200009
Abstract: the objective of this article is to circumscribe the concept of subject that emerges from neuroscientific theories of the mind. we approach three key authors from the field of neuroscience: francisco varela, humberto maturana, and gerald edelman. based on an analysis of their work, we conclude that a given concept of subject, founded on an entirely materialist view of the mind, emerges as an advantageous substitute for what we could call a "psychological" concept, based principally but not exclusively on psychoanalysis. we discuss the hypothesis that the current success of the "neuroscientific" vision of human beings, while in fact pointing to some degree of break with the so-called "modern paradigm", may also be interpreted as the radicalization of given characteristics and contradictions present in the constitution of this paradigm itself and, consequently, that of the modern person.
Rede ONSA e o Projeto Genoma Humano do Cancer: Contribui o ao Genoma Humano
Kimura Edna T.,Baía Gilson S.
Arquivos Brasileiros de Endocrinologia & Metabologia , 2002,
Abstract: A contribui o maior da ciência brasileira ao genoma humano foi trazida pelo Projeto Genoma Humano do Cancer (Human Genome Cancer Project - HCGP) uma parceria da FAPESP e do Ludwig Institute for Cancer Research e desenvolvido por 29 diferentes laboratórios de seqüenciamento e um centro de bioinformática. Foram seqüenciados mais de 1 milh o de fragmentos gênicos expressos (expressed sequences tags, ESTs), provenientes de diferentes tumores humanos. Grande parte destes dados é de acesso público através da website do Gene Bank (www.ncbi.nlm.nig.gov), mantido pelo NCBI - National Center for Biotechnology Information. Atualmente, diversos projetos est o em desenvolvimento utilizando informa es geradas no HCGP e abrangem observar a express o diferenciada dos genes em diferentes tumores, caracteriza o completa de genes específicos, assim como o estudo funcional e estrutural dos produtos protéicos. é promissora a perspectiva de que num futuro próximo, diferentes resultados provenientes destas investiga es possam trazer benefícios preventivos, prognósticos e clínicos em cancer e outras doen as.
Evaluation of Local Spices as Biopesticides for the Control of Ootheca mutabilis, Shalbera and Clavigralla tomentosicollis (Stal.) on Cultivated Cowpea (Vigna unguiculata L.) in Nigeria
Ime O. Udo,Edna A. Akpan
Journal of Agricultural Science , 2012, DOI: 10.5539/jas.v4n10p7
Abstract: Two experiments were conducted at the University of Uyo Teaching and Research Farm in 2003 and 2004 to evaluate the impact of aqueous extracts of five local spices as biopesticides for the control of O. mutabilis and C. tomentosicollis on cultivated cowpea (Vigna unguiculata L.). The spices evaluated were Capsicum annum L., Xylopia aethiopica (Dunal), Piper guineense Schum and Thonn, Allium sativum L. and Zingiber officinale Roscoe. The extracts were applied at 3, 4 and 5% concentrations, respectively to study toxicity, repellency, pod and leaf damage as well as grain yield. The experiment was designed as a Randomized Complete Block Design and replicated three times. Results obtained showed that the biopesticides significantly (P<0.05) reduced insect population, offered greater protection and promoted better grain yield compared to the control. However, amongst the spices, P. guineense consistently induced greater protection by bringing down the population of insects and promoting grain yield. The promising use of spices as biopesticides is discussed since they are available local resources for poor farmers and are environmentally friendly options for pest control.
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