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Search Results: 1 - 10 of 860682 matches for " Mendon?a D.M.F. "
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Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Mendona, D.M.F.;Chimelli, L.;Martinez, A.M.B.;
Brazilian Journal of Medical and Biological Research , 2005, DOI: 10.1590/S0100-879X2005000600015
Abstract: amyotrophic lateral sclerosis (als), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. there is evidence for the accumulation of neurofilaments (nf) in motor neurons of the spinal cord in als cases. nf are major structural elements of the neuronal cytoskeleton. they play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. they are composed of three different polypeptides: light (nf-l), medium (nf-m) and heavy (nf-h) subunits. in the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of nf and the presence of each subunit in control and als cases. spinal cords from patients without neurological disease and from als patients were obtained at autopsy. in all als cases there was a marked loss of motor neurons, besides atrophic neurons and preserved neurons with cytoplasmic inclusions, and extensive gliosis. in control cases, the immunoreaction in the cytoplasm of neurons was weak for phosphorylated nf-h, strong for nf-m and weak for nf-l. in als cases, anterior horn neurons showed intense immunoreactivity in focal regions of neuronal perikarya for all subunits, although the difference in the integrated optical density was statistically significant only for nf-h. furthermore, we also observed dilated axons (spheroids), which were immunopositive for nf-h but negative for nf-m and nf-l. in conclusion, we present qualitative and quantitative evidence of nf-h subunit accumulation in neuronal perikarya and spheroids, which suggests a possible role of this subunit in the pathogenesis of als.
Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis
Mendona D.M.F.,Chimelli L.,Martinez A.M.B.
Brazilian Journal of Medical and Biological Research , 2005,
Abstract: Amyotrophic lateral sclerosis (ALS), a neurodegenerative disease of unknown etiology, affects motor neurons leading to atrophy of skeletal muscles, paralysis and death. There is evidence for the accumulation of neurofilaments (NF) in motor neurons of the spinal cord in ALS cases. NF are major structural elements of the neuronal cytoskeleton. They play an important role in cell architecture and differentiation and in the determination and maintenance of fiber caliber. They are composed of three different polypeptides: light (NF-L), medium (NF-M) and heavy (NF-H) subunits. In the present study, we performed a morphological and quantitative immunohistochemical analysis to evaluate the accumulation of NF and the presence of each subunit in control and ALS cases. Spinal cords from patients without neurological disease and from ALS patients were obtained at autopsy. In all ALS cases there was a marked loss of motor neurons, besides atrophic neurons and preserved neurons with cytoplasmic inclusions, and extensive gliosis. In control cases, the immunoreaction in the cytoplasm of neurons was weak for phosphorylated NF-H, strong for NF-M and weak for NF-L. In ALS cases, anterior horn neurons showed intense immunoreactivity in focal regions of neuronal perikarya for all subunits, although the difference in the integrated optical density was statistically significant only for NF-H. Furthermore, we also observed dilated axons (spheroids), which were immunopositive for NF-H but negative for NF-M and NF-L. In conclusion, we present qualitative and quantitative evidence of NF-H subunit accumulation in neuronal perikarya and spheroids, which suggests a possible role of this subunit in the pathogenesis of ALS.
Aberrant crypt foci and colon cancer: comparison between a short- and medium-term bioassay for colon carcinogenesis using dimethylhydrazine in Wistar rats
Rodrigues M.A.M.,Silva L.A.G.,Salvadori D.M.F.,de Camargo J.L.V.
Brazilian Journal of Medical and Biological Research , 2002,
Abstract: Aberrant crypt foci (ACF) in the colon of carcinogen-treated rodents are considered to be the earliest hallmark of colon carcinogenesis. In the present study the relationship between a short-term (4 weeks) and medium-term (30 weeks) assay was assessed in a model of colon carcinogenesis induced by dimethylhydrazine (DMH) in the rat. Six-week-old male Wistar rats were given subcutaneous injections of DMH (40 mg/kg) twice a week for 2 weeks and killed at the end of the 4th or 30th week. ACF were scored for number, distribution pattern along the colon and crypt multiplicity in 0.1% methylene-blue whole-mount preparations. ACF were distinguished from normal crypts by their larger size and elliptical shape. The incidence, distribution and morphology of colon tumors were recorded. The majority of ACF were present in the middle and distal colon of DMH-treated rats and their number increased with time. By the 4th week, 91.5% ACF were composed of one or two crypts and 8.5% had three or more crypts, while by the 30th week 46.9% ACF had three or more crypts. Thus, a progression of ACF consisting of multiple crypts was observed from the 4th to the 30th week. Nine well-differentiated adenocarcinomas were found in 10 rats by the 30th week. Seven tumors were located in the distal colon and two in the middle colon. No tumor was found in the proximal colon. The present data indicate that induction of ACF by DMH in the short-term (4 weeks) assay was correlated with development of well-differentiated adenocarcinomas in the medium-term (30 weeks) assay.
Aberrant crypt foci and colon cancer: comparison between a short- and medium-term bioassay for colon carcinogenesis using dimethylhydrazine in Wistar rats
Rodrigues, M.A.M.;Silva, L.A.G.;Salvadori, D.M.F.;de Camargo, J.L.V.;Montenegro, M.R.;
Brazilian Journal of Medical and Biological Research , 2002, DOI: 10.1590/S0100-879X2002000300010
Abstract: aberrant crypt foci (acf) in the colon of carcinogen-treated rodents are considered to be the earliest hallmark of colon carcinogenesis. in the present study the relationship between a short-term (4 weeks) and medium-term (30 weeks) assay was assessed in a model of colon carcinogenesis induced by dimethylhydrazine (dmh) in the rat. six-week-old male wistar rats were given subcutaneous injections of dmh (40 mg/kg) twice a week for 2 weeks and killed at the end of the 4th or 30th week. acf were scored for number, distribution pattern along the colon and crypt multiplicity in 0.1% methylene-blue whole-mount preparations. acf were distinguished from normal crypts by their larger size and elliptical shape. the incidence, distribution and morphology of colon tumors were recorded. the majority of acf were present in the middle and distal colon of dmh-treated rats and their number increased with time. by the 4th week, 91.5% acf were composed of one or two crypts and 8.5% had three or more crypts, while by the 30th week 46.9% acf had three or more crypts. thus, a progression of acf consisting of multiple crypts was observed from the 4th to the 30th week. nine well-differentiated adenocarcinomas were found in 10 rats by the 30th week. seven tumors were located in the distal colon and two in the middle colon. no tumor was found in the proximal colon. the present data indicate that induction of acf by dmh in the short-term (4 weeks) assay was correlated with development of well-differentiated adenocarcinomas in the medium-term (30 weeks) assay.
Mioepitelioma benigno: presentación de un caso clínico
Ostrosky,A.; Villa,D.M.; González,M.; Klurfan,F.;
Revista Espa?ola de Cirugía Oral y Maxilofacial , 2007, DOI: 10.4321/S1130-05582007000500006
Abstract: we present a case of benign myoepithelioma of the parotid gland, which was treated by superficial gland lobectomy along with facial nerve conservation. a differential diagnosis with other benign tumors with similar clinical signs such as pleomorphic adenoma is made. the importance of a correct anatomopathologic diagnosis is also stressed, as is making a differential diagnosis with epi-myoepithelial carcinomas.
Community Based Management System For The Universal Immunisation Programme
Chand Dyal A,Khale M,Jadhavar F,Chowdhary D.M
Indian Journal of Community Medicine , 1990,
Abstract:
Mioepitelioma benigno: presentación de un caso clínico Benign myoepithelioma: presentation of a case report
A. Ostrosky,D.M. Villa,M. González,F. Klurfan
Revista Espa?ola de Cirugía Oral y Maxilofacial , 2007,
Abstract: Se presenta un caso de mioepitelioma benigno de glándula parótida, el cual fue tratado mediante la lobectomía superficial de la glándula, con conservación del nervio facial. Se hace el diagnóstico diferencial con otros tumores benignos como el adenoma pleomorfo, con el cual comparten una clínica similar. También se destaca la importancia de un correcto diagnóstico anatomopatológico, en este caso haciendo diagnóstico diferencial con los carcinomas epi-mioepiteliales. We present a case of benign myoepithelioma of the parotid gland, which was treated by superficial gland lobectomy along with facial nerve conservation. A differential diagnosis with other benign tumors with similar clinical signs such as pleomorphic adenoma is made. The importance of a correct anatomopathologic diagnosis is also stressed, as is making a differential diagnosis with epi-myoepithelial carcinomas.
Qualidade microbiológica de queijo-coalho comercializado em Aracaju, SE
Santana, R.F.;Santos, D.M.;Martinez, A.C.C.;Lima, á.S.;
Arquivo Brasileiro de Medicina Veterinária e Zootecnia , 2008, DOI: 10.1590/S0102-09352008000600031
Abstract: the occurrence of staphylococcus aureus, salmonella spp., and microorganisms indicators of fecal contamination in "coalho"cheese commercialized in 15 retail store at the central market of aracaju, se during four months was determined. sixteen samples (26.7%) were positive for salmonella spp. and 28 (46.7%) for coagulase positive staphylococci. in relation to contamination by total coliforms, 56 samples (93.3%) presented values from 8.0 x102 to 1.23 x104nmp/g; and for thermal tolerant coliforms, from 2.72 x102 to 1.12 x103nmp/g. these values are not acceptable for the brazilian legislation for "coalho" cheese. this indicates that the analyzed products were inappropriate to the human consumption.
The effect of feed supplementation on the onset of puberty in Brazilian dairy heifers
Meirelles, C.F.;Abdalla, A.L.;Vitti, D.M.S.S.;
Scientia Agricola , 1994, DOI: 10.1590/S0103-90161994000200026
Abstract: most brazilian dairy production is conducted by small holders whose general management skills and feeding programs are often deficient. one common problem directly attributed to underfeeding is that heifers rarely reach sexual maturity before 15 months of age. groups of growing heifers were treated to detect the effects of protein supplementation and antihelmintic treatment on sexual maturity. the first ovulation occurred at 513 ± 44 and 573 ± 36 days (p<.01) in supplemented and control groups, respectively. liveweight gains from start of the trial to the first ovulation were 378 ±.02 and 331 ±.04 g for suplemented (s) and control (c) groups. only 52.17 % of heifers in c group (11 of 23) but 95.65 % in s group (22 of 23) reached sexual maturity before the age of 18 mo (p< .01). animals with anthelmintic treatment had better growth performance than the controls.
Effect of Human Activities and Oil Pollution on the Microbiological and Physicochemical Quality of Udu River, Warri, Nigeria
H.S.A. Aluyi,F.O. Ekhaise,D.M. Adelusi
Journal of Applied Sciences , 2006,
Abstract: The effect of human activities and oil pollution on the microbiological quality of Udu River was investigated from September 2002 to January 2003. The microbiological parameters were carried out using the standard microbiological techniques. The bacterial and fungal loads were shown to be highest in the inhabited stations (2 and 3) with downstream (station 4) suffering from dilution effects of human activities and upstream (station 1) showing lower counts. Stations 2 and 3 were shown to record the highest heterotrophic microbial load 10.7 x 108 cfu mL-1 and 10.4 x 108 cfu mL-1 and 8.0 x 108 cfu mL-1 and 9.0 x 108 cfu mL-1 for bacterial and fungal isolates respectively. Total coliform counts ranges from 1.5 - 4.3 x 106 and 1.4 - 2.8 x 106 MPN100 mL-1 for stations 2 and 3. Microorganism isolated and characterized includes nine bacterial and four fungal genera. The bacterial isolates were Staphylococcus, Streptococcus, Proteus, Escherichia, Clostridium, Acetobacter, Salmonella, Pseudomonas, Micrococcus and the predominant fungal isolates includes, Aspergillus, Penicillium, Geotrichum and Helminthosporium. The concentrations of total suspended solids, Biochemical Oxygen Demand (BOD5), conductivity, total iron, total solids, total copper and total dissolved solids were shown to be higher in stations 2 and 3 than station 1 which have no direct human activities. Similarly, the values obtained for salinity, total hardness and Dissolved Oxygen (DO) of the inhabited stations were shown to be lower than station 1. Correlation coefficient shows positive relationship between the different microbial populations and some physicochemical parameters studied.
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