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A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the
starting strain CGMCC3090 with ultraviolet
irradiation and lithium chloride. The NHase activity
of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml),
as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in
seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).