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Search Results: 1 - 10 of 21 matches for " Meiothermus ruber "
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The sectionalized DNA shuffling: an effective tool for molecular directed evolution of Meiothermus ruber TreS
分段DNA shuffling: 一种大分子海藻糖合酶有效的定向进化方法

Liu Yan-Chao,Wang Yu-Fan,Qian Ke-Fan,Zhang Jun,Xiao Chen-Peng,Xing Lai-Jun,Li Ming-Chun,

微生物学通报 , 2013,
Abstract: Objective] The gene M-treS from Meiothermus ruber CBS-01 encodes a trehalose synthase of 962 amino acids, named M-TreS. To improve its catalytic activity, we constructed a method of molecular directed evolution, the sectionalized DNA shuffling. Methods] Through two PCR steps with two pairs of partially complementary primers, the M-treS gene was parted into two sections. After the two sections shuffled respectively, a whole gene was obtained through the complementarity of the primers. This method was more feasible, with higher mutability than normal DNA shuffling. Results] Mutants were obtained after one round of the sectionalized DNA shuffling, in combination with error-prone PCR. The best mutant enzyme contained 6 amino acid substitutions, whose catalytic activity and efficiency were 1.6-fold and 2-fold of that of the wild type, respectively. In the 6 amino acid substitutions, 5 were caused by homologous recombination, and one by error-prone PCR. Conclusion] This study indicates that the sectionalized DNA shuffling is an effective tool for molecular directed evolution of macromolecular proteins.
Cloning, expression and functional analysis of the genes in TPS/TPP trehalose synthetic pathway of Meiothermus ruber

Yueming Zhu,Yichen Tang,Hengyi Xu,Juan Zhang,Dongsheng Wei,Laijun Xing,Mingchun Li,

生物工程学报 , 2009,
Abstract: By constructing the genomic DNA library of Meiothermus ruber CBS-01, the genes of trehalose phosphate synthase (TPS) and trehalose phosphate phosphatase (TPP) involved in trehalose synthesis were cloned. The genes were cloned into the plasmid pET21a, and expressed in Escherichia coli Rosetta gami (DE3). The activities of these two purified enzymes were confirmed by thin layer chromatography (TLC). Meanwhile, we tested the cellular compatible solutes of M. ruber CBS-01 under different environmental pressure,...
When the skin meets the bowel: About a case  [PDF]
Ganzetti Giulia, Campanati Anna, Di Sario Antonio, Rubini Corrado, Zizzi Antonio, Tarantino Giuseppe, Benedetti Antonio, Offidani Annamaria
Open Journal of Gastroenterology (OJGas) , 2013, DOI: 10.4236/ojgas.2013.36052
Abstract: Ulcerative colitis (UC) is a chronic relapsing inflammatory disease of the colorectal region which may be complicated by extraintestinal manifestations, as cutaneous involvement. Immune dysregulation resulting in a lymphocyte mediated destructive process has been suggested as a possible pathogenetic link between gut and skin-related manifestations. The authors describe an atypical clinical aspect of lichen ruber planus in a patient affected by vitiligo, alopecia areata and ulcerative colitis.
Isolation and Biochemical Characterization of Rubelase, a Non-Hemorrhagic Elastase from Crotalus ruber ruber (Red Rattlesnake) Venom
Yumiko Komori,Kaname Sakai,Katsuyoshi Masuda,Toshiaki Nikai
Toxins , 2011, DOI: 10.3390/toxins3070900
Abstract: A novel non-hemorrhagic basic metalloprotease, rubelase, was isolated from the venom of Crotalus ruber ruber. Rubelase hydrolyzes succinyl-L-alanyl-L-alanyl-L-alanyl p-nitroanilide (STANA), a specific substrate for elastase, and the hydrolytic activity was inhibited by chelating agents. It also hydrolyzes collagen and fibrinogen. However, hemorrhagic activity was not observed. By ESI/Q-TOF and MALDI/TOF mass spectrometry combined with Edman sequencing procedure, the molecular mass of rubelase was determined to be 23,266 Da. Although its primary structure was similar to rubelysin (HT-2), a hemorrhagic metalloprotease isolated from the same snake venom, the circumstances surrounding putative zinc binding domain HEXXHXXGXXH were found to be different when the three-dimensional computer models of both metalloproteases were compared. The cytotoxic effects of rubelase and rubelysin on cultured endothelial and smooth muscle cells were also different, indicating that the substitution of several amino acid residues causes the changes of active-site conformation and cell preference.
Management of used tires, accomplishments in the world, and situation in Serbia
Stanojevi? Du?an D.,Rajkovi? Milo? B.,To?kovi? Dragan V.
Hemijska Industrija , 2011, DOI: 10.2298/hemind110729061s
Abstract: It is estimated that all over the world there are more than 550 million road vehicles in everyday use. Annually, 1.3 billion used tyres are dismantled from these vehicles due to safety reasons. A small number of these tyres end up reused as second-hand tyres or, after being retreated, as new ones. The rest are end of life tyres which are recycled or used as tire derived fuels under controlled combustion conditions. Modern vehicle tyres are a high-technological product containing, on the average, 85% hydrocarbon, 10-15% steel, as well as some other chemical materials. Tyres belong to high caloric materials, with calorific value of a passenger car tyre of approx. 30.2 MJ/kg being more than of hard coal and comparable to calorific value of petrol-coke. Having this in mind many countries use end of life tyres as safe alternative fuel in cement works or power plants. Undeveloped countries dump end of life tyres on legal and illegal scrap stockpiles. End of life tyres, if deposed correctly, don’t endanger the environment because they are chemically inert. However, since they can easily be burnt there is a real possibility of tyre dump fires, which in effect can lead to harmful products emission that extremely pollute the environment. End of life tyres represent a very important secondary material, which when recycled is transformed into a number of raw materials used in rubber industry, for road construction, sports facilities, residential and business buildings, artificial fish habitats in shallow seas, sea walls, steel production, etc. By the industrial procedures of controlled degradation, which have been uneconomical up till now, gaseous and liquid fuel can be obtained by natural rubber, steel and soot recycling. The countries of the EU, together with Norway and Switzerland, were the world leaders in the field of effective end of life tyre management in the year 2009. Out of 2.62 million tonnes of end of life tyres, 95% were restored or used as energents, and 5% settled on dumps. About 26.000 t of used tyres are collected in Serbia each year. In accordance with adopted legal legislations, up to 30% can be used as tire derived fuels while 70% are being recycled. Serbia belongs to the group of countries which have solved the problem of used tyres in a modern way, and in accordance with EU Legislation practice.
Vibrio ruber (S2A1), a Marine Bacterium that Exhibits Significant Antimicrobial Activity
Wan Norhana, N.,Darah, I.
Malaysian Journal of Microbiology , 2005,
Abstract: A potential antimicrobial-producing marine bacterium, designated as S2A1, was isolated from a seagrass collected in Setiu Lagoon, Terengganu. S2A1 was a Gram negative rod that was motile by means of a polar flagellum. Phenotypic and genotypic characterisation indicated that strain S2A1 represented a species in the genus Vibrio. The antimicrobial activities of S2A1 against a number of test microorganisms showed a broad antimicrobial spectrum property with inhibition towards 25 out of 29 test microorganisms. The antimicrobial compound(s) of S2A1 was more effective against Gram-positive bacteria with 100% inhibition, compared to yeast (88.8%) and Gram-negative bacteria (75.0%) tested. High activity scores were observed when using whole cells compared to cell free extract.
Floral visitors of Aechmea constantinii (Mez) L. B. Sm. (Bromeliaceae) in a remnant of the Brazilian Northeast Atlantic Rainforest
Petrúcio Alexandre Fonseca Rios J,uliana Braga da Silva,Flávia de Barros Prado Moura
Biotemas , 2010,
Abstract: This study aims to determine the floral visitors and potential pollinators of Aechmea constantinii (Mez) L. B. Sm. (Bromeliaceae), a bromeliad endemic to the Brazilian Northeast Atlantic Rainforest. Reproductively-active individuals were observed systematically and their visitors were recorded and determined. The main recorded fl oral visitors were Glaucis hirsutus, Phaethornis ruber and Phaethornis pretrei (hummingbirds) which executed frontal functional floral visits in which they touched the parts of the fl owers. Visits of Plebeia flavocincta, Plebeia sp., Trigona spinipes and Euglossa cordata (bees) and Talides sergestu and Strymon ziba (butterflies) were also recorded. In addition, two ants (Hymenoptera, Insecta, Formicidae) were identifi ed in activity on the fl oral scapes and flowers of the studied bromeliad. The suggestion is made in the study that the A. constantinii is pollinated by hummingbirds since these birds executed direct frontal visits to the fl owers, touching reproductive structures. The identification of pollen on the bodies of bees and butterflies, as well as the contact executed by visitors, with the stigma of the visited flowers, offered an indication that these species may exert an influence as secondary pollinators of Aechmea constantinii.
Breeding of NHase hyper-producing Rhodococcus ruber strain LUV30-06 and verification of mutants by RAPD  [PDF]
Shiwei Wang, Min Wang, Qinghui Wang
American Journal of Molecular Biology (AJMB) , 2013, DOI: 10.4236/ajmb.2013.32015

A nitrile hydratase (NHase) hyper-producing Rhodococcus ruber strain LUV30-06 was bred by mutagenization on the starting strain CGMCC3090 with ultraviolet irradiation and lithium chloride. The NHase activity of the strain LUV30-06 was increased by 21.99% (3881.3 U/ml), as compared with that of R. ruber CGMCC3090 (3181.4 U/ml). The mutant strain UV30-06 has been proved genetically stable with higher NHase activity in seven successive subcultures as well as random amplified polymorphic DNA analysis (RAPD).

Amplia??o da distribui??o de quatro espécies de morcegos (Mammalia, Chiroptera) no Rio Grande do Sul, Brasil
Weber, Marcelo de Moraes;Arruda, Jeferson Luis Steindorff de;Cáceres, Nilton Carlos;
Biota Neotropica , 2007, DOI: 10.1590/S1676-06032007000200032
Abstract: the objective of this paper is to record the occurrence of four bats species in the central of the rio grande do sul state, south of brazil: histiotus montanus, pygoderma bilabiatum, nyctinomops laticaudatus and myotis ruber. data were obtained from the scientific collection of universidade federal de santa maria (ufsm) and represent distribution extensions for these bat species in this state. we present information on the habitat where the species were collected, showing that some of them are new for this species. some of the studied species may be considered rare in the rio grande do sul state, whereas others should have their degree of threat revaluated.
Descri??o da larva de Scinax similis (Cochran) com notas comparativas sobre o grupo "ruber" no sudeste do Brasil (Amphibia, Anura, Hylidae)
Alves, Ana C.R;Carvalho e Silva, Sérgio Potsch de;
Revista Brasileira de Zoologia , 1999, DOI: 10.1590/S0101-81751999000200016
Abstract: the larva of scinax similis (cochran, 1952) is described from ilha do fund?o (rio de janeiro, rio de janeiro state, brazil). comparisons with other species of the group that occur in southeastern brazil are added. the larva of s. similis can be distinguished by the following diagnostic features: (1) eyes large in relation to body length (17,3%), body height (26,8%), and body width (29,7%), interocular distance three times larger than eye diameter; (2) lower beak with two transverse stripes, proximal half white and distal half black.
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