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Search Results: 1 - 10 of 1412 matches for " Masayuki Nagasawa "
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Epstein-Barr-Virus-Positive B-Cell Lymphoma of Recipient Origin Despite of the Elimination of Clonally EBV-Infected T Cells by Allogeneic Stem Cell Transplantation in a Patient with Chronic Active EBV Infection
Masayuki Nagasawa
Case Reports in Transplantation , 2012, DOI: 10.1155/2012/164824
Abstract: A 20-year-old patient with chronic active EBV infection (CAEBV) received peripheral blood stem cell transplantation (PBSCT) from HLA-one-locus-mismatched mother. Although EB-virus-infected T cells were eliminated after PBSCT, she developed EB-virus-positive B-cell lymphoma of recipient origin in the brain. By reducing the immunosuppressive therapy, the initial lesion disappeared. However, another lesion in the opposite lateral brain appeared later and was resistant to further reduction of immunosuppressive therapy. EBV-DNA was persistently negative after PBSCT in the peripheral blood. This case is suggestive in management of EBV reactivation after SCT and understanding alloimmune response to EBV.
Epstein-Barr-Virus-Positive B-Cell Lymphoma of Recipient Origin Despite of the Elimination of Clonally EBV-Infected T Cells by Allogeneic Stem Cell Transplantation in a Patient with Chronic Active EBV Infection
Masayuki Nagasawa
Case Reports in Transplantation , 2012, DOI: 10.1155/2012/164824
Abstract: A 20-year-old patient with chronic active EBV infection (CAEBV) received peripheral blood stem cell transplantation (PBSCT) from HLA-one-locus-mismatched mother. Although EB-virus-infected T cells were eliminated after PBSCT, she developed EB-virus-positive B-cell lymphoma of recipient origin in the brain. By reducing the immunosuppressive therapy, the initial lesion disappeared. However, another lesion in the opposite lateral brain appeared later and was resistant to further reduction of immunosuppressive therapy. EBV-DNA was persistently negative after PBSCT in the peripheral blood. This case is suggestive in management of EBV reactivation after SCT and understanding alloimmune response to EBV. 1. Introduction Epstein-Barr virus (EBV) is a ubiquitously spread herpes family virus, which infects more than 90% of adults [1]. Primary EBV infection often is asymptomatic and sometimes presents as acute febrile mononucleosis. EBV usually infects B cells and latently infected B cells are regulated by potent T cells [2]. In an immunodeficient state, these B cells occasionally induce lymphoproliferative disease, which is sometimes life threatening [3–5]. Chronic active EBV infection (CAEBV) is another EBV-related lymphoproliferative disease in which EBV infects T or NK cells [6, 7]. Although the pathogenesis is not well understood, the prognosis is poor in the long run and allogeneic stem cell transplantation (allo-HSCT) is recommended to eradicate infected T or NK cells [8]. With an increased transplantation-related mortality by conventional myeloablative conditioning regimen (MAC), reduced intensity conditioning regimen (RIC) has been applied, resulting in an improved 3-year overall survival of in RIC compared to in MAC [9]. Recently, the author has reported that level of serum granulysin is useful for discriminating NK cell type CAEBV from T cell type [10]. The author has experienced a patient with CAEBV who developed EBV-positive B-cell lymphoma of recipient origin after allogeneic stem cell transplantation. The clinical course of this patient is suggestive to understand the significance of allo-immune response against EBV-associated lymphoproliferative diseases (EBV-LPD) in the setting of allo-HSCT. 2. Case Report The patient presented with mosquito hypersensitivity at the age of five. Thereafter, she had recurrent cervical lymphadenopathy with fever, which resolved spontaneously in a couple of weeks. When she was 14 years old, systemic lymphadenopathy with fever developed. Biopsy of cervical lymph node revealed the diagnosis of Hodgkin lymphoma. She
A Pediatric Case of Systemic Lupus Erythematosus Developed 10 Years after Cord Blood Transplantation for Juvenile Myelomonocytic Leukemia
Masayuki Nagasawa,Yuki Aoki
Case Reports in Transplantation , 2012, DOI: 10.1155/2012/619126
Abstract:
A Pediatric Case of Systemic Lupus Erythematosus Developed 10 Years after Cord Blood Transplantation for Juvenile Myelomonocytic Leukemia
Masayuki Nagasawa,Yuki Aoki
Case Reports in Transplantation , 2012, DOI: 10.1155/2012/619126
Abstract: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is a most powerful immunotherapy for hematological malignancies. However, the impact of immunological disturbances as a result of allo-HSCT is not understood well. We experienced an 11-year-old boy who presented with systemic lupus erythemathosus (SLE) 10 years after unrelated cord blood transplantation of male origin for juvenile myelomonocytic leukemia (JMML) with monosomy 7. Bone marrow examination showed complete remission without monosomy 7. Genetic analysis of peripheral blood revealed mixed chimera with recipient cells consisting of <5% of T cells, 50–60% of B cells, 60–75% of NK cells, 70–80% of macrophages, and 50–60% of granulocytes. Significance of persistent mixed chimera as a cause of SLE is discussed. 1. Introduction Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an effective treatment to cure the hematological malignancy through the alloimmune reaction. However, excessive alloimmune reaction triggers acute graft versus host disease (aGVHD) and it attacks various organs and induces their dysfunction, which is often life-threatening. Chronic GVHD is based on more insidious alloimmune reaction and its clinical symptoms sometimes mimic autoimmune diseases. In allo-HSCT, donor cells usually replace the bone marrow cells completely, which is called complete chimera. Donor and recipient hematopoietic cells sometimes coexist stably, which is called mixed chimera. Although mixed chimera is a sign of relapse of hematological malignancy, there are cases of stable and persistent chimera without relapse. However, the impact of immunological disturbances as a result of allo-HSCT and mixed chimera is not understood well. We had an 11-year-old boy who presented with SLE 10 years after allogeneic cord blood transplantation. Interestingly, most of T cells were of donor origin, but B cells, NK cells, macrophages, and granulocytes were mixed chimera. 2. Case Report At 6-month-old, he presented with hepatosplenomegaly and skin eruption. Bone marrow examination revealed monosomy 7 and he was diagnosed as juvenile myelomonocytic leukemia through clinical evaluation. At 15-month-old, he received HLA-DRB1 one locus mismatched male-derived cord blood transplantation. Conditioning regimen was myeloablative (busulfan (140?mg/m2??× 4) + etoposide (15?mg?/kg/day × 4) + cyclophosphamide (60?mg/kg × 2) + antithymocyte globulin (2.5?mg/kg × 4)). GVHD prophylaxis was cyclosporine and short-term methotrexate. SCT was successful with only mild GVHD. Complete chimera was determined on day 41.
Seasonal Variation in a Hymenopterous Parasitoid, Holcotetrastichus rhosaces (Walker) (Hymenoptera: Tetrastichinae), on Its Hosts, Cassida nebulosa L. and C. piperata Hope (Coleoptera: Chrysomelidae), in Sendai, Northeastern Area of Japan  [PDF]
Atsuhiko Nagasawa
Advances in Entomology (AE) , 2014, DOI: 10.4236/ae.2014.24026
Abstract: To investigate the seasonal variation of parasitism on Cassida nebulosa L. and C. piperata Hope, this research was conducted at the experimental field of Tohoku University in Sendai, Japan, where these tortoise beetles were parasitized by hymenopterous parasitoid Holcotetrastichus rhosaces (Walker). Percentage parasitism started to increase from the middle of July and reached nearly 100% in August. Adults of H. rhosaces emerged from the pre-pupae of the tortoise beetles, with 6 to 30 adults emerging per pre-pupae. The second generation of C. nebulosa and C. piperata was heavily parasitized by H. rhosaces. The adults of these tortoise beetles were rarely found in the field during late summer due to the high rates of parasitism.
Food Preference of Pecky Rice Bugs Is Influenced by Experience  [PDF]
Atsuhiko Nagasawa
Advances in Entomology (AE) , 2016, DOI: 10.4236/ae.2016.42012
Abstract: This study investigated whether prior experience influences the plant food preference of Trigonotylus caelestialium and Stenotus rubrovittatus which cause pecky rice grain, by using rice plants and 2 poaceous weeds. In a choice experiment between Digitaria ciliaris and the rice plants, both T. caelestialium and S. rubrovittatus adults that were reared on D. ciliaris plants showed significant initial preference for D. ciliaris over rice. In a choice experiment between Echinochloa crus-galli var. aristata and rice plants, T. caelestialium adults reared on E. crus-galli var. aristata strongly preferred E. crus-galli var. aristata over rice throughout the experiment. However, at and after 24 h, T. caelestialium adults reared on rice showed no food preference, although T. caelestialium initially preferred E. crus-galli var. aristata. In contrast, S. rubrovittatus adults reared on rice showed no preference between E. crus-galli var. aristata and rice. However, S. rubrovittatus reared on E. crus-galli var. aristata initially preferred E. crus-galli var. aristata to rice, with this preference waning with time. The same results were obtained for both sexes. Although the effect of experience differed with food source and the species of mirid bug, prior experience initially had a noticeable effect, which disappeared with time (1 d).
Synthesis of Macrocyclic Hexaoxazole (6OTD) Dimers, Containing Guanidine and Amine Functionalized Side Chains, and an Evaluation of Their Telomeric G4 Stabilizing Properties
Keisuke Iida,Masayuki Tera,Takatsugu Hirokawa,Kazuo Shin-ya,Kazuo Nagasawa
Journal of Nucleic Acids , 2010, DOI: 10.4061/2010/217627
Abstract: Structure-activity relationship studies were carried out on macrocyclic hexaoxazole (6OTD) dimers, whose core structure stabilizes telomeric G-quadruplexes (G4). Two new 6OTD dimers having side chain amine and guanidine functional groups were synthesized and evaluated for their stabilizing ability against a telomeric G4 DNA sequence. The results show that the 6OTD dimers interact with the DNA to form 1:1 complexes and stabilize the antiparallel G4 structure of DNA in the presence of potassium cation. The guanidine functionalized dimer displays a potent stabilizing ability of the G4 structure, as determined by using a FRET melting assay ( ? ). 1. Introduction G-quadruplexes (G4), secondary DNA structures consisting of G-quartet planers in G-rich regions, play significant biological roles for example, control of transcription and telomeric lengths [1–19]. One typical G4 forming DNA sequence is a telomere, which exists at the ends of chromosomes consisting of repeating single-stranded sequences [1–12]. Telomeres protect chromosomes from end to end fusion events, which result in replication of the chromosome (the Hayflick limit) [20]. The telomere repeats are elongated by the reverse transcriptase telomerase, which is overexpressed in most tumor cells. In contrast, telomerase activity is not observed in normal somatic cells [21]. Since the activity of this enzyme is inhibited by the G4 structure of telomeres owing to steric hindrance, small molecules that selectively bind and stabilize the telomeric G4 should be potential anticancer agents. As a result, a number of G4 ligands, inspired by artificial DNA intercalators as well as natural products, have been developed during the past decade [22]. Telomestatin (TMS) is a natural product isolated from Streptomyces anulatus 3533-SV4, which displays one of the most potent telomeric G4 binding activity (Figure 1) [23–28]. Interaction analysis has shown that two molecules of TMS induce conversion of telomeric G4 into an antiparallel type by way of an end stacking mode [25–28]. We have recently developed macrocyclic hexaoxazole compounds 6OTD, containing a variety of side chain functional groups, that serve as a novel TMS derivative [29–32]. In addition, by considering the proposed binding mode of TMS with telomeric G4, we have carried out further structural development of dimeric 6OTD derivatives (Figure 1) [33]. The results of molecular dynamics calculations guided the selection of 6OTD dimer 1 that contains an appropriate length of a linker between the monomeric units of 6OTD. Studies showed that dimer 1 binds to
Modulation of p53 activity by IκBα: Evidence suggesting a common phylogeny between NF-κB and p53 transcription factors
David H Dreyfus, Masayuki Nagasawa, Erwin W Gelfand, Lucy Y Ghoda
BMC Immunology , 2005, DOI: 10.1186/1471-2172-6-12
Abstract: Using previously published structure data, we noted a significant degree of structural alignment between p53 and NF-κB p65. We also determined that IκBα and p53 bind in vitro through a specific interaction in part involving the DNA binding region of p53, or a region proximal to it, and the amino terminus of IκBα independently or cooperatively with the ankyrin 3 domain of IκBα In cotransfection experiments, κBα could significantly inhibit the transcriptional activity of p53. Inhibition of p53-mediated transcription was increased by deletion of the ankyrin 2, 4, or 5 domains of IκBα Co-precipitation experiments using the stably transfected ankyrin 5 deletion mutant of κBα and endogenous wild-type p53 further support the hypothesis that p53 and IκBα can physically interact in vivo.The aggregate results obtained using bacterially produced IκBα and p53 as well as reticulocyte lysate produced proteins suggest a correlation between in vitro co-precipitation in at least one of the systems and in vivo p53 inhibitory activity. These observations argue for a mechanism involving direct binding of IκBα to p53 in the inhibition of p53 transcriptional activity, analogous to the inhibition of NF-κB by κBα and p53 by 53BP2/ASPP2. These data furthermore suggest a role for ankyrin proteins in the regulation of p53 activity. Taken together, the NFκB and p53 proteins share similarities in structure, DNA binding sites and binding and regulation by ankyrin proteins in support of our hypothesis that the two proteins share common descent from an ancestral transcriptional factor.In this work we present evidence that the p53 tumor suppressor and NF-κB transcription factors could be related through common descent from a family of ancestral transcription factors regulating cellular proliferation and apoptosis. P53 and related proteins are transcription factors that regulate DNA repair and cellular apoptosis in response to stress and injury, notably those resulting in DNA damage [1-4]. Although
Analysis of Psychological Factors That Influence Preference for Luxury Food and Car Brands Targeting Japanese People  [PDF]
Kazutoshi Fujiwara, Shin’ya Nagasawa
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.59059
Abstract: This study examines the strong preference among Japanese people for luxury brands and makes a comparative analysis of the psychological factors that influence the development of purchase intentions and how they differ between the food and car luxury brands. An empirical analysis was performed by using psychological factors that influence the preference for luxury brands, which Sugimoto [1] demonstrated in a study on Japanese people. The results suggested the following: 1) Differentiation from Others is an important factor in developing purchase intentions for both food and car luxury brands and is a particularly important factor for cars; 2) Conformity to Group Norms is not an important factor in developing purchase intentions for both food and car luxury brands in which consumers continue to feel a sense of rarity; 3) Avoiding Cognitive Dissonance is an important factor in developing purchase intentions for both food and car luxury brands; 4) Quality Evaluation is an important factor in developing purchase intentions for food luxury brands only.
Relationships among Purchase Intentions for Luxury Brands and Personality Traits Based on the Big Five  [PDF]
Kazutoshi Fujiwara, Shin’ya Nagasawa
American Journal of Industrial and Business Management (AJIBM) , 2015, DOI: 10.4236/ajibm.2015.511063
Abstract: Fujiwara and Nagasawa [1] verified differences in the degree of effect of the four psychological factors1 that constituted Japanese people’s preference for luxury brands on the development of purchase intentions for luxury food and car brands. This study is related to the study mentioned above and verifies the effects of consumers’ personality traits on purchase intentions for these luxury brands. It uses a personality trait classification method called the “Big Five personality traits” to perform an empirical analysis of the way that the five dimensions of personality traits4 influence purchase intentions for each brand. As a result, it suggests 1) that the purchase intentions of emotionally unstable consumers with a high dimension of Neuroticism for FERRARI and PORSCHE are significantly lower than those with a low dimension of Neuroticism and 2) that the purchase intentions of consumers who are highly devoted to art and have a high dimension of Openness to Experience for DOM PERIGNON, FERRARI, ROLLS-ROYCE and PORSCHE are significantly higher than those with a low dimension of Openness to Experience.
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