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Search Results: 1 - 10 of 163369 matches for " Martin K?hl "
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QUANTIFICATION OF BIOFILMS IN MULTI-SPECTRAL DIGITAL1 VOLUMES FROM CONFOCAL LASER-SCANNING MICROSCOPES
Karsten Rodenacker,Andreas Brühl,Martina Hausner,Martin Kühn
Image Analysis and Stereology , 2000, DOI: 10.5566/ias.v19.p151-156
Abstract: Populations of bacteria in sludge flocs and biofilm marked by fluorescence marked with fluorescent probes are digitised with a confocal laser scanning microscope. These data are used to analyse the microbial community structure, to obtain information on the localisation of specific bacterial groups and to examine gene expression. This information is urgently required for an in-depth understanding of the function and, more generally, the microbial ecology of biofilms. Methods derived from quantitative image analysis are applied to digitised data from confocal laser scanning microscopes to obtain quantitative descriptions of volumetric, topological (and topographical) properties of different compartments of the components under research. In addition to free-moving flocs, also biofilms attached to a substratum in an experimental environment are analysed. Growth form as well as interaction of components are quantitatively described. Classical measurements of volume and intensity (shape, distribution) and distance dependent interaction measurements using methods from mathematical morphology are performed. Mainly image (volume) processing methods are outlined. Segmented volumes are globally and individually (in terms of 3Dconnected components) measured and used for distance mapping transform as well as for estimation of geodesic distances from the substrate. All transformations are applied on the 3D data set. Resulting distance distributions are quantified and related to information on the identity and activity of the probe-identified bacteria.
Improved success rate and stability for phase retrieval by including randomized overrelaxation in the hybrid input output algorithm
Martin Khl,A. A. Minkevich,Tilo Baumbach
Physics , 2012, DOI: 10.1364/OE.20.017093
Abstract: In this paper, we study the success rate of the reconstruction of objects of finite extent given the magnitude of its Fourier transform and its geometrical shape. We demonstrate that the commonly used combination of the hybrid input output and error reduction algorithm is significantly outperformed by an extension of this algorithm based on randomized overrelaxation. In most cases, this extension tremendously enhances the success rate of reconstructions for a fixed number of iterations as compared to reconstructions solely based on the traditional algorithm. The good scaling properties in terms of computational time and memory requirements of the original algorithm are not influenced by this extension.
Combined Pulse Electroporation – A Novel Strategy for Highly Efficient Transfection of Human and Mouse Cells
Thorsten Stroh,Ulrike Erben,Anja A. Kühl,Martin Zeitz,Britta Siegmund
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0009488
Abstract: The type of a nucleic acid and the type of the cell to be transfected generally affect the efficiency of electroporation, the versatile method of choice for gene regulation studies or for recombinant protein expression. We here present a combined square pulse electroporation strategy to reproducibly and efficiently transfect eukaryotic cells. Cells suspended in a universal buffer system received an initial high voltage pulse that was continuously combined with a subsequent low voltage pulse with independently defined electric parameters of the effective field and the duration of each pulse. At comparable viable cell recoveries and transfection efficiencies of up to 95% of all cells, a wide variety of cells especially profited from this combined pulse strategy by high protein expression levels of individual cells after transfection. Long-term silencing of gene expression by transfected small interfering RNA was most likely due to the uptake of large nucleic acid amounts as shown by direct detection of fluorochromated small interfering RNA. The highly efficient combined pulse electroporation strategy enables for external regulation of the number of naked nucleic acid molecules taken up and can be easily adapted for cells considered difficult to transfect.
Crime and Punishment with Habit Formation  [PDF]
Vladimir Kühl Teles, Joaquim Andrade
Theoretical Economics Letters (TEL) , 2011, DOI: 10.4236/tel.2011.13021
Abstract: Moral concepts affect crime supply. This idea is modelled assuming that illegal activities is habit forming. We introduce habits in an intertemporal general equilibrium framework to illegal activities and compare its outcomes with a model without habit formation. The findings are that habit and crime presents a non linear relationship that hinges upon the level of capital and habit formation. It is possible to show that while the effect of habit on crime is negative for low levels o habit formation it becomes positive as habits goes up. Secondly habit reduces the marginal effect of illegal activities return on crime. Finally, the effect of habit on crime depends positively on the amount of capital. This could explain the relationship between size of cities and illegal activity.
Characterization of Chromosomal Instability in Murine Colitis-Associated Colorectal Cancer
Marco Gerling,Rainer Glauben,Jens K. Habermann,Anja A. Kühl,Christoph Loddenkemper,Hans-Anton Lehr,Martin Zeitz,Britta Siegmund
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0022114
Abstract: Patients suffering from ulcerative colitis (UC) bear an increased risk for colorectal cancer. Due to the sparsity of colitis-associated cancer (CAC) and the long duration between UC initiation and overt carcinoma, elucidating mechanisms of inflammation-associated carcinogenesis in the gut is particularly challenging. Adequate murine models are thus highly desirable. For human CACs a high frequency of chromosomal instability (CIN) reflected by aneuploidy could be shown, exceeding that of sporadic carcinomas. The aim of this study was to analyze mouse models of CAC with regard to CIN. Additionally, protein expression of p53, beta-catenin and Ki67 was measured to further characterize murine tumor development in comparison to UC-associated carcinogenesis in men.
Choques cambiais, política monetária e equilíbrio externo da economia brasileira em um ambiente de hysteresis
Teles, Vladimir Kühl;
Economia Aplicada , 2005, DOI: 10.1590/S1413-80502005000300004
Abstract: the concept of hysteresis suggests that a shock on exchange rate change the foreign equilibrium of the economy. in that context, the experience of the fluctuations of the brazilian case from the real plan is examined, obtaining three central conclusions: 1) a high interest rate monetary policy diminishes the magnitude of the impact of an exchange depreciation about the exports, what imply a shift of the external equilibrium; 2) an elevation of the interest rate increase the lag of the j curve for the brazilian economy; 3) the change of the exchange rate regime from fixed to flexible in 1999, altered the parameters of the stochastic process that governs the exchange fluctuations, affecting the entrance decision parameters in the external market of the domestic firms, being necessary a bigger exchange depreciation to the domestic firm decide to export.
O tratamento das superfícies arquitet?nicas como problema teórico da restaura??o
Kühl, Beatriz Mugayar;
Anais do Museu Paulista: História e Cultura Material , 2004, DOI: 10.1590/S0101-47142004000100021
Abstract: the article deals with the question of architectural surfaces and their relation with restoration theories. the historical transformations undergone by restoration theories are examined in broad lines. the contemporary tendencies of restoration theories are also analyzed in order to verify the principles that should guide the interventions. the dissociation between theory and practice arises through the analysis of the practice of interventions in architectural surfaces. in this text, the author seeks to place an emphasis on the relevance of reflection on theoretical principles in order to face problems in their full intricacy, and as an efficient means of preserving the material, historic, formal and symbolic values of monuments.
Notas sobre a Carta de Veneza
Kühl, Beatriz Mugayar;
Anais do Museu Paulista: História e Cultura Material , 2010, DOI: 10.1590/S0101-47142010000200008
Abstract: this paper presents a critical reading of the venice charter, an icomos key document, fruit of a conference held in 1964. the charter is often quoted in brazil but is not always properly understood. the conservation and restoration charters - especially those produced by international institutions - are documents that have an indicatory or, at the most, prescriptive character. they constitute the deontological foundation of many professionals involved in preservation, but they are not recipes for immediate use. in order to elaborate a well-founded reading of the document, its ideas must be understood in connection to the theoretical postulates of the time they were engendered and to the developments of the field. thus this paper will examine these subjects, commenting and enlightening the charter's articles and pointing out the origins of specific ideas. it also discusses how the charter relates to previous documents and their theoretical foundations. this approach, based in a critical analysis, is necessary in order to reach a fuller interpretation of the charter's indications so that they can be used in the present.
Sebastian Scheele: Geschlecht, Gesundheit, Gouvernementalit t. Selbstverh ltnisse und Geschlechterwissen in der M nnergesundheitsf rderung. Sulzbach im Taunus: Ulrike Helmer Verlag 2010.
Jutta Kühl
querelles-net , 2011,
Abstract: Wenn es um ihre Gesundheit geht, gelten M nner mittlerweile als M ngelwesen: Sie essen lieber Fleisch als Salat, lassen ihr Auto regelm iger warten als ihren K rper und trinken auch gerne mal ein Glas zu viel. Die Zielgruppe Mann wird in der Gesundheitsf rderung zunehmend in den Blick genommen. Sebastian Scheeles detaillierte Analyse zeichnet erstmalig nach, welches Geschlechterwissen dabei (re)produziert wird. Zudem hinterfragt er die in der M nnergesundheitsf rderung propagierten Selbstverh ltnisse kritisch und stellt sie in den Kontext neoliberaler Regierungsweisen. Dafür erweitert er Foucaults Konzept der Gouvernementalit t um sozialkonstruktivistische Geschlechtertheorie und verknüpft diese mit den Gesundheitswissenschaften. When it comes to health, men are, by now, considered to be deficient beings: they prefer meat over salads, they get their cars checked more regularly than their bodies, and they like to have one over the eight from time to time. The target audience “men” is considered increasingly in health promotion. Sebastian Scheele’s detailed analysis is the first one to trace the gender knowledge that is (re)produced in this process. Furthermore, he questions the self-relations that are propagated in men’s health promotion and places them within the context of neoliberal ways of government. To this end, he expands Foucault’s concept of governmentality with social-constructivist gender theory and relates it to health sciences.
Gender und Milit r Gender and the Military
Jutta Kühl
querelles-net , 2004,
Abstract: Dieser Band der Heinrich-B ll Stiftung enth lt aktuelle internationale Forschungsergebnisse zum Verh ltnis von Gender und Milit r. Das Themenspektrum in den Beitr gen reicht von der Frage nach der Repr sentation von Frauen in den jeweiligen Streitkr ften bis zu Untersuchungen zur gesellschaftlichen Rolle des Milit rs bei der Konstruktion von Gender. This volume of the Heinrich-B ll Foundation contains results of current international research on the relationship between gender and the military. The thematical spectrum of the contributions ranges from the question of the representation of women in armed forces to studies on the societal role of the military for the construction of gender.
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