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Search Results: 1 - 10 of 29 matches for " Marlus Chorilli "
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Development and Validation of an LC-MS/MS Method for Quantitative Analysis of Mirtazapine in Human Plasma  [PDF]
Marlus Chorilli, Rudy Bonfilio, Caroline Rossim Louvandini, Francine Andressa Rosa Madia Gon?alves, Hérida Regina Nunes Salgado
American Journal of Analytical Chemistry (AJAC) , 2011, DOI: 10.4236/ajac.2011.26074
Abstract: Mirtazapine (MTZ) is an antidepressant drug, which belongs to the chemical class of piperazinoazepines. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantifica- tion of MTZ in plasma at the concentrations associated with therapy. Diazepam (DZP) was used as internal standard, added to 200 μL of plasma sample prior to a liquid-liquid extraction using hexane. Chroma- tographic separation was achieved on an Agilent® Eclipse XDB C-18 column (100 × 2.1 mm, 3.5 μm) in iso- cratic mode at 40?C. Mobile phase was 10 mM ammonium acetate/acetonitrile/formic acid (60/40/0.1, v/v/v) at a constant flow rate of 0.5 mL?min–1. The injection volume was 10 ?L and the total run time was 3.2 min. The method shows selectivity and linearity. The detection and quantitation limits were established at 0.17 and 0.50 ng?mL–1, respectively. The extraction recoveries for MTZ and DZP were found to be between 84.9 and 93.9%. The intra-day and inter-day precision and accuracy fulfill at the international acceptance criteria. The method shows to be stable for the studied parameters. Therefore, a rapid, specific, and sensitive LC-MS/MS method for quantification of MTZ in human plasma was developed and can be used in therapeu- tic drug monitoring of this drug.
Validation of a HPLC Method for Determination of Glutamine in Food Additives Using Post-Column Derivatization  [PDF]
Marlus Chorilli, Hérida Regina Nunes Salgado, Fabiana De Santana Santos, Lucélia Magalh?es Da Silva
American Journal of Analytical Chemistry (AJAC) , 2012, DOI: 10.4236/ajac.2012.32016
Abstract: An ion-exchange chromatography (IEC) followed by post-column derivatization method was validated for the determination of glutamine in food additive products. The chromatographic method was carried out on a sodium cation-exchange column and using detection at 570 nm. The mobile phase consisted of sodium eluant, pH 3.15, with 5% sulfolane, sodium eluant, pH 7.40 and sodium column regenerant. The method was linear in the range of 4 - 50 μg/mL (r2 = 0.9999). The accuracy was 99.78%. Moreover, method validation demonstrated acceptable results for precision and robustness. The method was found to be able to use for routine analysis of the glutamine.
Estudo de perfil de dissolu o dos medicamentos de referência, genérico e similar contendo cefalexina na forma farmacêutica cápsula
Marlus Chorilli,Ademir Alves Souza,Felipe Corrêa,Hérida Regina Nunes Salgado
Revista de Ciências Farmacêuticas Básica e Aplicada , 2010,
Abstract: Com o avan o da tecnologia e das pesquisas envolvendo libera o de fármacos, moderniza o de testes e maior ênfase da previsibilidade de efeitos terapêuticos por meio dos testes in vitro, os testes de dissolu o e os estudos de perfis de dissolu o têm ganhado cada vez mais importancia. Apesar de terem sido introduzidos inicialmente como uma forma de caracterizar o perfil de libera o de fármacos pouco solúveis, atualmente os testes de dissolu o fazem parte das monografias de quase todas as formas farmacêuticas sólidas orais. Este trabalho teve como objetivo estudar o perfil de dissolu o (porcentagem de fármaco dissolvido versus tempo) dos medicamentos de referência, genérico e similar contendo cefalexina 500 mg na forma farmacêutica de cápsulas. Foram analisadas três especialidades farmacêuticas (referência, genérico e similar), os quais foram submetidos ao teste de dissolu o e perfil de dissolu o in vitro. Os resultados obtidos no teste de dissolu o permitem concluir que as amostras encontraram-se de acordo com as especifica es e o perfil de dissolu o deste medicamento, nesta forma farmacêutica, é considerado de dissolu o rápida (85% de fármaco dissolvido em 15 minutos). Portanto, existe uma grande semelhan a entre as curvas obtidas, o que sugere que se trata de equivalentes farmacêuticos. Palavras-chave: Cefalexina. Perfil de dissolu o. Medicamento referência. Medicamento genérico. Medicamento similar. ABSTRACT Study of dissolution profile of the pioneer brand name, generic and similar brand name pharmaceutical products containing cephalexin in the form of capsules With recent advances in technology and research into drug delivery, the modernization of tests and greater emphasis on the predictability of therapeutic effect by means of in vitro tests, the dissolution test and the study of dissolution profiles are gaining more and more importance. Though introduced initially as a way of characterizing the release profile of poorly soluble drugs, dissolution tests are currently part of pharmacopoeial monographs on almost all the oral solid pharmaceutical forms. The objective of this study was to determine the dissolution profile (percent drug dissolved versus time) of the pioneer brand, generic and similar pharmaceutical capsules containing 500mg cephalexin. Three pharmaceutical brands (reference, generic and similar) were subjected to the dissolution test and in vitro dissolution profiles were recorded. From the results of the dissolution test, it was concluded that the samples met the acceptance criterion, as no difference was observed in the
Verifica o da atividade antibacteriana de sabonete líquido contendo extrato glicólico de Dimorphandra mollis Benth.
Ketylin Fernanda Migliato,Marlus Chorilli,Maria Virginia Scarpa,Raquel Regina Duarete Moreira
Revista de Ciências Farmacêuticas Básica e Aplicada , 2009,
Abstract: Dimorphandra mollis Benth., Compositae, falsobarbatim o, é utilizada topicamente como cicatrizante, adstringente e antimicrobiano. No presente estudo, verificou-se a atividade antibacteriana de sabonete líquido contendo extrato glicólico de D. mollis (EGD) em diferentes concentra es (8, 15 e 20%) e em diferentes pHs (6 e 8). Foram preparadas cinco formula es (F) de sabonete: F1 - triclosan (0,1%), F2 - EGD (8%), F3 - EGD (15%), F4 - EGD (20%) e F5 - sem conservante. Cascas de D. mollis foram secas em estufa de ar circulante e pulverizadas. Os extratos brutos foram preparados por turbo-extra o utilizando-se etanol. Após filtra o, os extratos foram concentrados em evaporador rotatório, liofilizados e ressuspendidos em propilenoglicol para a obten o do extrato glicólico. A atividade antibacteriana foi verificada pelo método de difus o em ágar, empregando cilindros em placa. Placas contendo Staphylococcus aureus, Pseudomonas aeruginosa e Escherichia coli foram incubadas a 37oC durante 24 horas. Após incuba o, as leituras foram realizadas com paquímetro, observando-se o diametro do halo de inibi o de crescimento bacteriano. Verificouse que o sabonete líquido contendo triclosan provocou inibi o do crescimento bacteriano em ambos os pHs; já os sabonetes sem conservante e contendo EGD, independente da concentra o e do pH empregados, n o apresentaram atividade antibacteriana. Palavras-chave: Atividade antibacteriana. Sabonete líquido. Dimorphandra mollis Benth.
Desenvolvimento de sistemas líquido-cristalinos empregando silicone fluido de co-polímero glicol e poliéter funcional siloxano
Chorilli, Marlus;Prestes, Paula Souza;Rigon, Roberta Balansin;Leonardi, Gislaine Ricci;Chiavacci, Leila Aparecida;Scarpa, Maria Virgínia;
Química Nova , 2009, DOI: 10.1590/S0100-40422009000400035
Abstract: for the construction of the phase diagrams, the method of the aqueous titration was used. there were prepared 5 ternary diagrams, varying the surfactant and the oil phase. the liquid-crystalline phases were identified by polarized light microscopy. the formulations prepared with silicon glycol copolymer, polyether functional siloxane (pfs) and water (s1) and with diisopropyl adipate, pfs and water (s4) presented liquid-crystalline phases with lamellar arrangement. moreover, after 15 days in hot oven (37 oc), the formulations presented hexagonal arrangement, evidencing the influence of the temperature in the organization of the system.
Tornando o "Jogo Possível": Reflex es sobre a Pedagogia do Esporte, os fundamentos dos jogos desportivos coletivos e a aprendizagem Making the “Possible Game”: Reflections on Sport Pedagogy, Fundamentals of Team Sports and Sport Learning Haciendo el "juego posible": reflexiones sobre la Pedagogía del deporte, los fundamentos de los juegos deportivos colectivos y el aprender deportivo
Rubens Venditti Jr,Marlus Alexandre Sousa
Pensar a Prática , 2008, DOI: 19806183/rpp.v11i1.1796
Abstract: Este trabalho surge da necessidade de discuss es sobre o papel educacional do profissional de educa o física na aprendizagem esportiva e Pedagogia do Esporte. Relacionamos correntes teóricas da Pedagogia do Esporte com o ensino dos jogos desportivos coletivos. Para tanto, utilizamos como instrumento o jogo, ferramenta fundamental de ensino e oportunidade social, através de suas características lúdicas. O jogo pode contribuir para o desenvolvimento de valores éticos, sociais e morais, assim como para a constru o de metáforas que estabele am rela es entre o esporte e a vida, fazendo uso da ludicidade e do ensino reflexivo. Ambos fundamentam nosso embasamento teórico para a atua o em Pedagogia do Esporte, trazendo mais oportunidades, op es de escolha e reflex es sobre nossa atua o profissional. This research work derives from the need to discuss the educational role of the physical education professional in sport learning and sport pedagogy. We have related sport pedagogy theories with the teaching of team sports. We have used the game as an instrument which is a fundamental teaching tool as well as a social opportunity, through its playful characteristics. Games can contribute to the development of ethical, social, and moral values, and to the building of metaphors that can establish relations between sport and life, making use of playfulness and reflexive teaching. Both concepts are fundamental to our theoretical foundation and bring more opportunities, choice, and reflections to our professional work. Keywords: sport pedagogy – sport initiation – game – reflexive teaching Este trabajo aparece de la necesidad de discusiones a respecto del papel educativo del profesional de educación física en la ense anza deportiva y Pedagogía del deporte. Relacionamos las corrientes teóricas de la pedagogía del deporte con la ense anza de los juegos colectivos. Para eso, utilizamos como instrumento el juego, la herramienta básica de la ense anza y la oportunidad social, por medio de sus características lúdicas. El juego puede contribuir en el desarrollo de valores éticos, sociales y morales; como también en la construcción de metáforas que establecen relaciones entre el deporte y la vida, haciendo uso de lo lúdico y la educación reflexiva. Ambos fundamentan nuestro basamiento teórico para el funcionamiento en Pedagogía del deporte, trayendo más oportunidades, opciones de elección y reflexiones en nuestra actuación profesional. PALABRAS-CLAVE: Pedagogía del deporte – iniciación deportiva – juego – educación reflexiva.
Population dynamics of Drepanotrema cimex (Moricand, 1839) (Mollusca: Basommatophora: Planorbidae) in Barigüi Park, Curitiba City, State of Paraná, Brazil
Marlus Bueno-Silva,Marta Luciane Fischer
Biotemas , 2005,
Abstract: The ecology of the freshwater snail Drepanotrema cimex (Moricand, 1839), an intermediate host of trematode larvae, is little known in Brazil. We analyzed the population dynamics of D. cimex in Barigüi Park, Curitiba, Brazil. Collections for the survey were realized at three sites that were at different distances from the Barigüi River and had distinct water plant species. In total, 278 snails were collected, and the highest sampling rate was where Paspalum sp. and Alternanthera philoxeroides (Mart.) Griseb. occurred. A higher occurrence of D. cimex in the autumn suggested that the adult stage culminates in this season, resulting from the previous rainy period that seems to support the reproduction of snails in the summer. However, the water temperature had no correlation with the total quantity of snails. The mean shell diameter did not show any correlation either with the water temperature or with the seasons. We recorded intraspecific variations in the color of the shells among the collections from the different sites.
Animais de laboratório: o camundongo
M. Chorilli,D. C. Michelin,H. R.N. Salgado
Revista de Ciências Farmacêuticas Básica e Aplicada , 2009,
Abstract: A experimenta o animal nas pesquisas científicas tem contribuído sobremaneira para o desenvolvimento da ciência e tecnologia, promovendo ao longo dos anos a descoberta de medidas profiláticas e tratamentos de enfermidades que acometem os seres humanos. Animais de várias espécies têm sido utilizados nos últimos tempos, sendo os camundongos os mais intensamente utilizados e os mais profundamente conhecidos cientificamente. O objetivo deste trabalho foi realizar um levantamento bibliográfico, incluindo os trabalhos do nosso grupo, sobre o emprego de camundongos na experimenta o animal, abordando sua biologia geral, fisiologia de reprodu o, sistemas de cria o, genética, habita o, alimenta o, manejo, dor e eutanásia, técnicas de risco desenvolvidas na experimenta o, coleta de sangue, experimentos farmacológicos e toxicológicos. Embora tendências atuais preconizem a utiliza o de métodos alternativos (estudos in vitro, culturas de células, etc.), os modelos animais, como os camundongos, apresentam como principal vantagem o fornecimento de informa es sobre o organismo como um todo, fato que n o é conseguido com outros métodos, o que ainda possibilita o seu emprego em pesquisas científicas. Palavras-chave: experimenta o animal; camundongo; biologia geral; técnicas de risco na experimenta o.
Transplante hepático sem transfus?o sanguínea
Nicoluzzi, Jo?o Eduardo Leal;Moreira, Marlus;Caron, Pedro Ernesto;
Revista do Colégio Brasileiro de Cirurgi?es , 2005, DOI: 10.1590/S0100-69912005000400014
Abstract: orthotopic liver transplantation (olt) usually requires large amounts of blood transfusion. reports of olt without transfusion are scarce and often associated to religious reasons. herein we report two cases of olt successfully managed without blood transfusion and not related to religious beliefs.
Manejo terapêutico do hepatocarcinoma fibrolamelar
Moreira, Marlus;Nicoluzzi, Jo?o Eduardo Leal;Caron, Pedro Ernesto;
Revista do Colégio Brasileiro de Cirurgi?es , 2005, DOI: 10.1590/S0100-69912005000200012
Abstract: fibrolamellar carcinoma of the liver is a distinct variant of hepatocellular carcinoma characterized by the lack of association with cirrhosis or other underlying liver diseases, as hepatitis b virus. the serum alpha-fetoprotein level is often not elevated, occuring typically in young patients. distinction from conventional hepatocellular carcinoma is of clinical significance, because prognosis is far better due to higher ressecability and more indolent growth. we describe a case of a 26-year-old woman free of other liver diseases presenting with a large abdominal palpable mass. after ressection by vascular exclusion technique, the pathologic examination revealed fibrolamellar carcinoma. the former should not be confused with the hepatocellular carcinoma, since it is related with a more favorable prognosis.
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