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Search Results: 1 - 10 of 11180 matches for " Mario; Juvier "
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Anticuerpos IgY en ensayos de látex-aglutinación
José Raúl Dopico,Mario álvarez,Ana Isabel Juvier,Janette Trujillo
Vaccimonitor , 2012,
Abstract: Los anticuerpos aviares (IgY) presentan algunas ventajas con relación a los anticuerpos IgG de mamíferos,debido a su fácil obtención y purificación y su bajo costo de producción. El objetivo de este trabajo fue estudiar la factibilidad de acoplar anticuerpos IgY a partículas de poliestireno y evaluar su desempe o en ensayos de látexaglutinación en lámina. Para este propósito se utilizó como modelo la detección del antígeno de superficie de la hepatitis B (HBsAg). Gallinas Leghorn se inmunizaron con el ingrediente farmacéutico activo que se utiliza en la elaboración de la vacuna recombinante cubana Heberbiovac-HB. Los anticuerpos obtenidos se purificaron y emplearon en la preparación de reactivos de látex-aglutinación (0,8 μm) para la detección del HBsAg, los cuales se evaluaron con 50 muestras de suero frente a un reactivo comercial similar. Los resultados se evaluaron de satisfactorios. Se demostró la factibilidad de acoplar anticuerpos aviares a partículas de látex y se abre una perspectiva al empleo de este tipo de ensayo para el diagnóstico rápido de diversas enfermedades.
Anticuerpos IgY en ensayos de látex-aglutinación
Dopico,José Raúl; álvarez,Mario; Juvier,Ana Isabel; Trujillo,Janette; Reyes,Giselle; Pico,María Cristina; Casadelvalle,Isis; Giraldino,Isabel;
Vaccimonitor , 2012,
Abstract: avian antibodies (igy) display some advantages in relation to the mammalian igg antibodies due to its easy procedure for generation and purification and besides for its lower production cost. the objective of this work was to analyze the feasibility of binding igy antibodies to polystyrene particles and to evaluate its performance in latex-agglutination tests in slide. for this purpose, the detection of hepatitis b surface antigen (hbsag) was used as a model. leghorn hens were immunized with the active pharmaceutical ingredient (mpa) used in the manufacturing of the cuban recombinant vaccine heberbiovac hb . the obtained antibodies were purified and used in the preparation of the latex particles (0.8 μm) for the detection of the hbsag by agglutination. the performance of the test was evaluated with 50 serum samples against similar commercial reagent. the results obtained were satisfactory and demonstrated the feasibility of binding such avian antibodies to latex particles. these results open a perspective to the use of this type of assay for the rapid diagnostic of many diseases.
Síndrome de Charles Bonnet en cuatro casos con atrofia óptica Charles Bonnet syndrome in four cases with optical atrophy
Lester Pola Alvarado,Yoel Rodríguez Martin,Tamara Juvier Riesgo,Dayamí Lazo Paez
Revista Cubana de Oftalmolog?-a , 2012,
Abstract: El síndrome de Charles Bonnet constituye un trastorno muy poco reportado. En Cuba, que se conozca, no hay referencia de casos publicados hasta este momento. Se caracteriza por la presencia de alucinaciones visuales complejas en individuos con pérdida visual, mentalmente sanos y con conciencia crítica de la naturaleza irreal de sus alucinaciones. Se presentan cuatro casos con este síndrome relacionados con déficit visual secundario a atrofia óptica. Solo el reconocimiento médico temprano de los síntomas del síndrome de Charles Bonnet y la posibilidad de brindar información, estrategias terapéuticas y asesoramiento adecuado, pueden beneficiar a estos pacientes en su nueva condición. Charles Bonnet syndrome is an underreported disorder. As far as we know, there are no publicized references in Cuba up to the present time. This syndrome involves complex visual hallucinations in visually impaired individuals, who are mentally healthy and have intact cognition of the unreal nature of their hallucinations. Four cases with this syndrome related to loss of vision due to optic atrophy were presented. The early recognition by clinicians of the symptoms of Charles Bonnet syndrome and the possibility of sharing proper information, therapeutic strategies and pieces of advice can help these patients suffering this disease.
Neuropatía óptica traumática Traumatic optic neuropathy
Eliecer Pérez García,Lester Pola Alvarado,Tamara Juvier Riesgo,Yoel Hernández Martín
Revista Cubana de Oftalmolog?-a , 2012,
Abstract: La neuropatía óptica traumática, en sus múltiples variantes, constituye una entidad poco frecuente. Se puede definir como aquella lesión del nervio óptico que aparece tras un episodio traumático. Puede clasificarse según su topografía y mecanismos productores. A pesar de un mejor entendimiento en los últimos a os de sus mecanismos productores, su tratamiento continúa siendo un tema extremadamente controversial. Traumatic optic neuropathy, in its different modalities, is a rare entity. It is the damage of the optic nerve resulting from a traumatic episode. The classification depends on its topography and causative mechanisms. Although there has been better understanding of the causative mechanisms in the last few years, the management is still an extremely controversial topic.
Different architectures of collagen fibrils enforce different fibrillogenesis mechanisms  [PDF]
Mario Raspanti
Journal of Biomedical Science and Engineering (JBiSE) , 2010, DOI: 10.4236/jbise.2010.312152
Abstract: According to current knowledge on collagen fibril-logenesis, collagen fibrils are formed by a cooperative process involving lateral fusion of small protofibrils. Almost all the experimental research, however, was carried out on tendon collagen, whose fibrils are characterized by approximately straight subfibrils. By contrast, in most tissues the collagen fibril sub-units follow a helical course in which geometrical constraints prevent lateral fusions, thereby implying a different mechanism where collagen fibrils grow by addition of individual microfibrils rather than by lateral fusion of pre-assembled subfibrils. The proc-ess at the origin of these fibrils may provide a simple, automatic explanation for the remarkable uniformity in fibrils size observed in most tissues without re-quiring the intervention of unknown mechanisms of diameter control. Other mechanisms of growth con-trol remain indispensable to terminate the fibril-logenesis process in tendons and ligaments.
Methods to Be Developed for Some First Applications of Mitochondrial Filamentation  [PDF]
Mario Gosalvez
Open Journal of Biophysics (OJBIPHY) , 2013, DOI: 10.4236/ojbiphy.2013.31A006
Abstract:

The new field of mitochondrial filamentation and its first potential applications are just getting underway (1-7), following a series of pilot experiments in our laboratory over the past few decades (8-14). I should like to give a brief overview of the role that some new methods are playing in this emerging area of physiological bioenergetics, supported by a few references to our contributions as I scarcely have the faculties now to understand other specific contributions. This new field is brimming with promises for medicine, science and society, in general. I cannot and must not be concrete or comprehensive, because I honestly feel that it is now that matters are really beginning to take off, assisted by the most appropriate international capabilities.

Metabolic control of respiration and glycolysis of tumoral cells  [PDF]
Mario Gosalvez
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.31011
Abstract:

The contribution of the author’s groups on the control of energy metabolism of cancer cells has been concisely reviewed. It is proposed that Otto Warburg’s “respiratory defect” of tumoral cells resides in an alteration of the recurrent filamentation cycle of mitochondria. These give those organelles an affinity lower for ADP than the affinity of the tumor cell isoenzyme of pyruvate kinase. These two findings may explain the essentials of the intimate mechanism of the aerobic glycolysis of cancer cells.

An experimental proposal for low order laboratory animals’ extension of metabolic life  [PDF]
Mario Gosalvez
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.36060
Abstract:

Essential bibliography, with therein references included, is presented owing to the contribution of the author groups to Mitochondrial Filamentation, which is a new emerging field of physiological energy metabolism. These studies provide the first seed concept for trials to extend the metabolic life, for a few days, in low order laboratory mammals killed by electrocution, as a first type of accidental death. It is proposed, essentially, to cool out the corpses very soon after death at 12oC-14oC and take advantage of the effect super magnetism to counteract the force of gravity to install a net recurrent cycle of oxygen consumption and oxygen production by filamented mitochondria in all the organism tissues. Once the cause of death had been corrected adequately, it is possible to try the reanimation to experience the full life of the corpse with highly sophisticated methodology.

 

Pre-Chelates, Post-Adducts and Implexes: A perspective proposal for the future of medicinal chemistry  [PDF]
Mario Gosalvez
Advances in Biological Chemistry (ABC) , 2013, DOI: 10.4236/abc.2013.36063
Abstract:

The title of this little assay coins three new words: Pre-Chelates, Post-Adducts and Implexes. These, very daring new excursions into the scientific English language, come from the developments of Quelamycin and Sodium L-Thioproline, through our different group studies arriving to cancer reversal by a dual strategy. It is enough to read all our published materials, cites and related papers, dealing more or less with the chemistry and possible mechanism of action of these compounds, to gather somewhat what signified our monomeric-triferric-doxorubicine, as possibly one of the first Pre-Chelates and our Sodium L-Thioproline, probably as one of the first Post-Adducts. Both molecules come to the human patients with cancer blood from the exterior. After this indication, it will be proposed a seed concept of a new type of molecules, the “Implexes” in which the metals will be embraced instead of being linked.

 

Reversal of Cancer by Dual Strategy?  [PDF]
Mario Gosalvez
Journal of Cancer Therapy (JCT) , 2013, DOI: 10.4236/jct.2013.42064
Abstract:

I present herewith a concise historic account of our development of the new technique for cancer therapy: Reversal of Cancer by Dual Strategy. This new method is based on the possible correction of the mitochondrial filamentation defect of tumors plus minor cytotoxicity addressed to the anoxic and fagocitic tumors redoubts, using the administration of the anticancer agents with a chrono-therapeutic emphasis on the patients mobility. Such methods probably will reach patients in the near future with the collaboration of private clinical trials mainly at least in the USA, Europe and Japan. Only the more essential references of this effort are provided.

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