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Search Results: 1 - 10 of 71488 matches for " Maria dM Vivanco "
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Stress relaxation microscopy (STREM): Imaging mechanical force decay in cells
Susana Moreno-Flores,Rafael Benitez,Maria dM Vivanco,Jose Luis Toca-Herrera
Physics , 2009,
Abstract: We have developed a novel scanning probe-based methodology to study cell biomechanics. The time dependence of the force exerted by the cell surface on a scanning probe at constant local deformation has been used to extract local relaxational responses. The generalized Maxwell viscoelastic model that accounts for multi relaxations fully describes the mechanical behaviour of the cell surface that exhibits a bimodal relaxation. Within the range of tested forces (0.1-4 nN) a slow and a fast relaxation with characteristic times of 0.1 and 1s have been detected and assigned to rearrangements in the cell membrane and cytoskeleton cortex, respectively. Relaxation time mapping allows to simultaneously detect non-uniformities in membrane and cytoskeletal mechanical behaviour and can be used as both identifying and diagnosing tools for cell type and cell disease.
Stress relaxation and creep experiments with the atomic force microscope: a unified method to calculate elastic moduli and viscosities of biomaterials (and cells)
Susana Moreno-Flores,Rafael Benitez,Maria dM Vivanco,Jose Luis Toca-Herrera
Quantitative Biology , 2010, DOI: 10.1088/0957-4484/21/44/445101
Abstract: We show that the atomic force microscope can perform stress relaxation and creep compliance measurements on living cells. We propose a method to obtain the mechanical properties of the studied biomaterial: the relaxation time, the elastic moduli and the viscosity.
Human Mammospheres Secrete Hormone-Regulated Active Extracellular Vesicles
Esperanza Gonzalez, Marco Piva, Eva Rodriguez-Suarez, David Gil, Felix Royo, Felix Elortza, Juan M. Falcon-Perez, Maria dM. Vivanco
PLOS ONE , 2014, DOI: 10.1371/journal.pone.0083955
Abstract: Breast cancer is a leading cause of cancer-associated death worldwide. One of the most important prognostic factors for survival is the early detection of the disease. Recent studies indicate that extracellular vesicles may provide diagnostic information for cancer management. We demonstrate the secretion of extracellular vesicles by primary breast epithelial cells enriched for stem/progenitor cells cultured as mammospheres, in non-adherent conditions. Using a proteomic approach we identified proteins contained in these vesicles whose expression is affected by hormonal changes in the cellular environment. In addition, we showed that these vesicles are capable of promoting changes in expression levels of genes involved in epithelial-mesenchymal transition and stem cell markers. Our findings suggest that secreted extracellular vesicles could represent potential diagnostic and/or prognostic markers for breast cancer and support a role for extracellular vesicles in cancer progression.
The devil is in the methods: lineage tracing, functional screens and sequencing, hormones, tumour-stroma interactions, and expansion of human breast tumours as xenografts
María dM Vivanco, John Stingl, Robert B Clarke, Mohamed Bentires-Alj
Breast Cancer Research , 2011, DOI: 10.1186/bcr3021
Abstract: The third international meeting of the European Network for Breast Development and Cancer (ENBDC) again promoted the sharing of protocols and ideas between groups working on breast development and cancer. Graduate students, postdocs and research associates were encouraged to attend. The following topics were covered in depth: functional screens and sequencing, hormones, lineage tracing, the propagation of human breast tumours as xenografts and tumour-stroma interactions.Chris Lord from the Breakthrough Breast Cancer Research Centre at the Institute of Cancer Research in London presented examples of the power of genome-wide functional screens. First, his group combined tamoxifen treatment of breast cancer cells in vitro and a small interfering RNA (siRNA) screen for kinases in an approach to identify events leading to tamoxifen sensitivity and tamoxifen resistance. They identified low cyclin-dependent kinase (CDK)10 expression as an important mediator of resistance to endocrine therapy in breast cancer. Knockdown of CDK10 blocked its inhibitory effect on ETS2, which in turn induced transcription of c-Raf and led to activation of the ERK/mitogen-activated protein kinase (MAPK) pathway and resistance to tamoxifen [1]. They also used a pooled genome-wide small hairpin RNA (shRNA) screen in the presence or absence of tamoxifen, coupled with massively parallel sequencing, and identified groups of genes the silencing of which increased sensitivity (for example C10orf72, C15orf55/NUT, EDF1, ING5, KRAS) or resistance (for example, BAP1, CLPP, GPRC5D, NAE1, NF1) to tamoxifen [2]. Second, they used a synthetic lethal unbiased shRNA screen for identifying sensitizers to a poly (ADP-ribose) polymerase (PARP) inhibitor in BRCA1 wild-type breast cancer cells. Knockdown of RAD51D, a novel ovarian cancer susceptibility gene, dramatically increased cell death upon PARP inhibition [3]. Third, they used a kinome-wide siRNA screen to identify genetic dependencies of breast cancer cell l
New methods in mammary gland development and cancer: proteomics, epigenetics, symmetric division and metastasis
Mohamed Bentires-Alj, Marina Glukhova, Nancy Hynes, Maria Vivanco
Breast Cancer Research , 2012, DOI: 10.1186/bcr3216
Abstract: Speaking of Weggis, where the European Network for Breast Development and Cancer (ENBDC) meeting is held, Mark Twain said 'Sunday in heaven is noisy compared to this quietness'. Indeed, people working on breast development and cancer took full advantage of this wonderfully quiet and beautiful place again this year for in-depth discussions. The meeting, held in April, started with an inspiring keynote lecture by Nancy Hynes and included the following sessions.The proteomics session consisted of two lectures, one by Bernd Bodenmiller (University of Zurich, Switzerland) and the other by Arzu Umar (Erasmus University Medical Center, Rotterdam, The Netherlands). Bodenmiller talked about mass cytometry, a new technology that combines flow cytometry and atomic mass spectrometry and that allows simultaneous measurement of up to 100 parameters [1]. This method allows the analysis of complex regulatory networks activated in response to various environmental signals at the single-cell level and can provide a system-wide picture of signaling events. Cellular signaling intermediates are recognized by antibodies, as used for other techniques, but become labeled with rare earth metals, non-radioactive and non-biological, instead of fluorochromes, and this generates a significantly larger range of reporter tags. In the mass cytometer, cells are introduced into the plasma, atomized, and analyzed by mass spectrometry. At present, a shortage of suitable antibodies against cellular signaling compounds is one of the limiting factors in mass cytometry. Nevertheless, the method significantly extends the capacities of currently used techniques and will play a central role in the analysis of complex cellular interactive networks [2].Umar presented a paper on the identification of a prognostic protein profile for triple-negative breast cancer. This group compared 25 triple-negative tumors that have a poor clinical prognosis with 38 tumors that have a more positive prognosis in order to defin
Veronica VIVANCO
The Turkish Online Journal of Distance Education , 2012,
Abstract: This paper reports on an experience carried out with second course students of the School of Aeronautical Engineers at the Polytechnic University of Madrid in the subject class Modern Technical Language. In the previous years the problem in that class had been the scarce participation of the students in the oral practices. They seemed to be lead and exclusively represented by a few students when it came to oral participation. The students proposed tackling recent research articles in which opinions could be discussed. The reading of these articles has risen new elements that work as language activators in the language classroom: critical reading and thinking have developed participation in the oral activities and produced a noticeable influence on their scientific and humanist thinking and behavior.From this, we may extract the consequence that the experience is not only related to scientific knowledge, since it has made them better speakers or speaking-counterparts and more class-participating and collaborative, which implies that the human, scientific and linguistic factors progress at the same time through the critical experience. This way, the exposition of their critical ideas has developed both their humanism and scientific mind. These two facets which seem to belong to far away fields become a bond in our experience: the process is scientific since it tries to be a systematic study of the knowledge fleshed in written texts and it is also humanist because it fulfils the students need to grow up as persons, that is absorbing knowledge and processing it in order to produce a new personal approach to the world.
Early Functional Deficit and Microglial Disturbances in a Mouse Model of Amyotrophic Lateral Sclerosis
Yannick Nicolas Gerber, Jean-Charles Sabourin, Miriam Rabano, Maria d M Vivanco, Florence Evelyne Perrin
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0036000
Abstract: Background Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder characterized by selective motoneurons degeneration. There is today no clear-cut pathogenesis sequence nor any treatment. However growing evidences are in favor of the involvement, besides neurons, of several partners such as glia and muscles. To better characterize the time course of pathological events in an animal model that recapitulates human ALS symptoms, we investigated functional and cellular characteristics of hSOD1G93A mice. Methods and Findings We have evaluated locomotor function of hSOD1G93A mice through dynamic walking patterns and spontaneous motor activity analysis. We detected early functional deficits that redefine symptoms onset at 60 days of age, i.e. 20 days earlier than previously described. Moreover, sequential combination of these approaches allows monitoring of motor activity up to disease end stage. To tentatively correlate early functional deficit with cellular alterations we have used flow cytometry and immunohistochemistry approaches to characterize neuromuscular junctions, astrocytes and microglia. We show that (1) decrease in neuromuscular junction's number correlates with motor impairment, (2) astrocytes number is not altered at pre- and early-symptomatic ages but intraspinal repartition is modified at symptoms onset, and (3) microglia modifications precede disease onset. At pre-symptomatic age, we show a decrease in microglia number whereas at onset of the disease two distinct microglia sub-populations emerge. Conclusions In conclusion, precise motor analysis updates the onset of the disease in hSOD1G93A mice and allows locomotor monitoring until the end stage of the disease. Early functional deficits coincide with alterations of neuromuscular junctions. Importantly, we identify different sets of changes in microglia before disease onset as well as at early-symptomatic stage. This finding not only brings a new sequence of cellular events in the natural history of the disease, but it may also provide clues in the search for biomarkers of the disease, and potential therapeutic targets.
Respuesta in vitro de fibroblastos de piel genital humana a andrógenos "In vitro" response of fibroblasts from genital skin to androgens
Mireya Fernandez G,Ximena Vivanco W,Marcela Palma M,Ana Maria Pino Z
Revista chilena de pediatría , 1996,
Anaerobic Mono-Digestion of Turkey Manure: Efficient Revaluation to Obtain Methane and Soil Conditioner  [PDF]
Rolando Chamy, Elba Vivanco, Carlos Ramos
Journal of Water Resource and Protection (JWARP) , 2011, DOI: 10.4236/jwarp.2011.38067
Abstract: This work demonstrates the possibility to make a full valuation of a solid waste such as turkey manure, to obtain methane and a soil conditioner/fertilizer from turkey manure anaerobic digestion in a mesophilic pilot-scale continuous stirred tank reactor at different organic loading rates (OLR) (from 0.5 to 2.5 kgVS/m3d). The application of the anaerobic mono-digestion for the turkey manure treatment was an efficient alternative, because high volatile solids removal and methane were obtained in addition to obtaining a stabilized solid waste that can be applied as soil conditioner, based on its nutritional parameters and humic substances content. In this way, the turkey manure anaerobic digestion can be applied avoiding the co-digestion of the manure with other wastes and allows a process devoid of pollutant emissions, obtaining two products. The reactor operation depends on the OLR, and its operation does not allow an OLR above 1.5 kgVS/m3d. Higher OLR produced a decrease in the TS and VS removals and methane productivity.
Metabolomic Profiling for Identification of Novel Potential Biomarkers in Cardiovascular Diseases
Maria G. Barderas,Carlos M. Laborde,Maria Posada,Fernando de la Cuesta,Irene Zubiri,Fernando Vivanco,Gloria Alvarez-Llamas
Journal of Biomedicine and Biotechnology , 2011, DOI: 10.1155/2011/790132
Abstract: Metabolomics involves the identification and quantification of metabolites present in a biological system. Three different approaches can be used: metabolomic fingerprinting, metabolic profiling, and metabolic footprinting, in order to evaluate the clinical course of a disease, patient recovery, changes in response to surgical intervention or pharmacological treatment, as well as other associated features. Characteristic patterns of metabolites can be revealed that broaden our understanding of a particular disorder. In the present paper, common strategies and analytical techniques used in metabolomic studies are reviewed, particularly with reference to the cardiovascular field.
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