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Search Results: 1 - 10 of 83867 matches for " Maria Teresa Quintero Martinez "
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Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico
Carolina Guadalupe Sosa-Gutierrez,Maria Teresa Quintero Martinez,Soila Maribel Gaxiola Camacho,Silvia Cota Guajardo,Maria D. Esteve-Gassent,María-Guadalupe Gordillo-Pérez
Journal of Veterinary Medicine , 2013, DOI: 10.1155/2013/797019
Abstract: Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico. 1. Introduction Ehrlichia canis is the causative agent of canine monocytic ehrlichiosis (CME). Moreover, CME is an emerging disease in veterinary medicine, and E. canis has been considered in the last decade as a potential zoonotic pathogen [1, 2]. It is a worldwide disease transmitted by a tick bite. The competent vector for its transmission is the Ixodidae ticks Rhipicephalus sanguineus and Dermacentor variabilis [3]. In dogs, the CME is a multiphase disorder that progresses in three stages: acute, subclinical, and chronic. Each phase is characterized by several clinical and hematologic abnormalities. Thrombocytopenia is a common finding in E. canis infected dogs and many clinicians tend to use it as an indication for antibiotic treatment, and it is observed in 84% of the cases and its severity varies in the different disease phases [4]. During the subclinical stage a moderate thrombocytopenia is observed, while the chronic phase is characterized by severe leukopenia and anemia. In this stage dogs show other complications such as hypocellular marrow, suppressed splenic sequestration, decreased life of platelets, and an increase of circulating migration-factor platelet inhibitor [3, 4]. The relationship between the magnitude of thrombocytopenia and prevalence of E. canis has been established in countries such as Brazil in 2004 where 84.1% of infected dogs showed thrombocytopenia [3]. Taken together, more data is necessary to
Seroprevalence of Ehrlichia canis in Dogs from a Mexico-U.S. Border Desert Region: Pilot Study
Luis Tinoco-Gracia,Hector Quiroz-Romero,Maria Teresa Quintero-Martinez,Tomas Benjamin Renteria-Evangelista,Alberto Barreras-Serrano,Sawako Hori-Oshima,Gilberto Lopez-Valencia,Alma Rossana Tamayo-Sosa,Victor Alejandro Quezada-iniguez,Manuel Moro,Javier Vinasco
Journal of Animal and Veterinary Advances , 2012,
Abstract: A pilot study was conducted to estimate the seroprevalence to Ehrlichia canis in dogs in a Mexico-U.S. border desert region. A total of 94 dogs were tested by Ehrlichia canis ELISA kit. Information of dogs regarding gender, age, size and intensity of tick infestation was collected and the significance of these class variables was determined by Chi-squared test. An adjusted prevalence of 49.3% (95% I.C. 30.8-54.1%) to Ehrlichia canis was obtained using the Rogan-Glanden estimator. Given that ehrlichiosis in dogs is a zoonotic disease and the seroprevalence in this desert region of the Mexicali-U.S border was found to be high, it is necessary to perform a formal study to establish the actual seroprevalence in both dogs and humans and determine the risk factors associated with ehrlichiosis. Since Ehrlichia canis is mostly transmitted by ticks Rhipicephalus sanguineus, preventive and control measures to erradicate ticks have to be established in order of minimize the risk of infection.
Prevalence and Risk Factors for Borrelia burgdorferi Infection in Dogs of Animal Control Centers from Mexicali, Baja California: A Mexico-US Border City
Luis Tinoco-Gracia,Hector Quiroz-Romero,Maria Teresa Quintero-Martinez,Tomas Benjamin Renteria-Evangelista,Alberto Barreras-Serrano,Sawako Hori-Oshima,Gilberto Lopez-Valencia,Alma Rossana Tamayo-Sosa,Gerardo Medina-Basulto,Paulina Haro-Alvarez,Manuel Moro,Javier Vinasco
Journal of Animal and Veterinary Advances , 2012,
Abstract: Lyme borreliosis, a worldwide zoonotic disease and characterized by polisystemic disorders, is caused by the spirochete B. burgdorferi, which is transmitted by a tick bite, primarily from Ixodes scapularis and I. pacificus. In Mexico, native Lyme disease has been recently reported in humans, while canine B. burgdorferi infection has been also reported in several areas of the country. In Monterrey, Mexico, a seroprevalence of 16% was observed in dogs (160/850) and molecular evidence was found in canine synovial fluid. Moreover, a preliminar study performed in 2003 in Mexicali, Mexico showed a prevalence of 7.4% (7/94) in dogs infested only by the tick Rhipicephalus sanguineus. The aim of this study was to estimate the seroprevalence of B. burgdorferi in dogs captured by personnel from the animal control centers in the city of Mexicali. Blood samples from 384 dogs were randomly selected from February 2005 to December 2006 and their sera were analized by the semiquantitative kit Borrelia burgdorferi ELISA Helica Biosystems, Inc., with 96% sensitivity and 95% specificity. An adjusted prevalence of 12% (95% IC 7.5-14.3%) was obtained using the Rogan-Gladen estimator. The seroprevalence obtained in this study was lower compared to those in Monterrey (16%) where the principal vector was Ixodes scapularis and in Sao Paulo, Brazil (15.6%) where the main vector was Amblyomma cajennense. No risk factors were associated with B. burgdorferi seropositivity. This study confirms the existence of B. burgdorferi past/present infection in dogs in an area where the only identified tick is R. sanguineus.
Seroprevalence of Borrelia burgdorferi in Dogs Froma a Mexico-U.S. Border Desert Rhegion: Pilot Study
Luis Tinoco-Gracia,Hector Quiroz-Romero,Maria Teresa Quintero-Martinez,Tomas Benjamin Renteria-Evangelista,Alberto Barreras-Serrano,Gilberto Lopez-Valencia,Sawako Hori-Oshima,Alma Rossana Tamayo-Sosa,Octavio Rico-Diez De Bonilla,Manuel Moro,Javier Vinasco
Journal of Animal and Veterinary Advances , 2012,
Abstract: The aim of this pilot study was to estimate the seroprevalence to Borrelia burgdorferi in dogs from a Mexico-U.S. border region. A total of 94 dogs were tested by Borrelia burgdorferi ELISA kit. Borreliosis or Lyme desease is a worldwide zoonotic tick-borne disease caused by the espirochete Borrelia burgdorferi This disease is characterized by arthritis, lameness, erythema migrans, fatigue, anorexia, general malaise, muscle pain, stiff neck, fever, heart block, kidney failure and neurological changes such as seizures, aggression. In some cases it is cause of death. The resullts show an adjusted prevalence to Borrelia burgdorferi of 8.2% (95% I.C. 1.5-13.3%), obtained using Rogan-Gladen estimator. Since B. burgdorferi is transmitted by ticks, preventive and control measures to erradicate ticks have to be established in order of minimize the risk of infection.
Direct pre-differentiation of rat mesenchymal stem cells into dopaminergic cells  [PDF]
Judith Zavala-Arcos, Maria Teresa Gonzalez-Garza, Janet Gutierrez-Alcala, Hector R. Martinez, Jorge E. Moreno-Cuevas
Stem Cell Discovery (SCD) , 2013, DOI: 10.4236/scd.2013.32018

The use of stem cells has been proposed as an alternative treatment for certain neurodegenerative disorders. It has also been suggested that in the pre-differentiated state, stem cells might provide a better therapeutic option than cells that are undifferentiated or fully differentiated. The purpose of this study was to develop a protocol aimed at reducing the incubation time required to induce the conversion of rat mesenchymal stem cells into immature dopaminergic neurons. Stem cells obtained from rat bone marrow were incubated in a control or induction media for 2-24 h. Cells incubated for 24 h in induction medium demonstrated an increase on the levels of the neuronal protein markers nestin, glial fibrillary acid protein, and β-tubulin III, as well as increases in the expression of Pax3, EN1, Thy1.1, and GEF10 genes. This manuscript presents evidence that adult mesenchymal cells are capable to respond, in a short time period, to a neuroinduction medium, and give raise to pre-differentiated neuron like cells representing an alternative for Parkinson disease cell therapy transplantation.

A Qualitative Acceleration Model Based on Intervals
ADCAIJ : Advances in Distributed Computing and Artificial Intelligence Journal , 2013,
Abstract: On the way to autonomous service robots, spatial reasoning plays a main role since it properly deals with problems involving uncertainty. In particular, we are interested in knowing people's pose to avoid collisions. With that aim, in this paper, we present a qualitative acceleration model for robotic applications including representation, reasoning and a practical application.
Small Polaron Localization, Jahn-Teller Distortion and Defects in La0.67Ca0.33MnO3: An Infrared Spectroscopy and X-ray Absorption Analysis
Nestor E. Massa,Helio C. N. Tolentino,Horacio Salva,Jose Antonio Alonso,Maria Jesus Martinez-Lope,Maria Teresa Casais
Physics , 2003,
Abstract: We combine infrared reflectivity and EXAFS (Extended X-ray Absorption Fine Structure) techniques to study the solid solution La0.67Ca0.33MnO3 prepared by different methods yielding samples with different insulator-metal transition temperatures (TIM). While the small polaron analysis of the optical conductivity provides a natural description for the higher frequency reflectivity tail of conducting samples, our structural results are in accord with two non-equivalent sites in the insulating phase of good-quality samples. Those sites, one for the Mn3+ Jahn-Teller distorted octahedra and another for the Mn4+ ion, gradually turn into one dynamically averaged below the transition TIM. On the other hand, carriers screening antiresonances near infrared longitudinal optical modes, above TIM, mirror thermal activated small polarons weakly smearing EXAFS oscillations. We associate this to the lack of M3+,M4+ explicit structure in the Mn K-edge absorption band. Extra octahedra, detected by EXAFS below TIM in higher resistivity samples, seem to be excluded of participating in the dynamics of the insulator-metal transition shifting TIM toward lower temperatures.
Phonon Activity and a Possible Lattice Arrangement in the Intermediate Phase of YVO3
Nestor E. Massa,Cinthia Piamonteze,Helio C. N. Tolentino,Jose Antonio Alonso,Maria Jesus Martinez Lope,Maria Teresa Casais
Physics , 2003,
Abstract: We show that in YVO3 new hard phonons gradually become zone center active below ~210 K verifying that a lattice phase transition takes place at about that temperature. The Debye-Waller factor found from EXAFS measurements show an additional disorder below 150K seen as a departure from the thermal behavior. We bring up the similarities of the intermediate phase to known results belonging to incommensurate systems that here we attribute to faulty orbital stacking with possible domain formation set below 116K, the onset temperature of the G-type antiferromagnetism. We found support for this interpretation in the inverted lambda shapes in the measured heat capacity at the phase transitions and in the overall temperature dependence of the reported new hard phonons as compared with those for known incommensurate dielectrics.
Response to Hydralazine-Valproate in a Patient with Mycosis Fungoides
Alfonso Due as-Gonzalez,Maria Teresa Vega,Déborah Martinez-Ba os,Linda García-Hidalgo,Pedro Sobrevilla
Case Reports in Medicine , 2010, DOI: 10.1155/2010/657579
Abstract: Histone deacetylase (HDAC) inhibitors have shown significant activity in the treatment of cutaneous T-cell lymphomas (CTCL). The epigenetic alterations of CTCL not only are limited to altered histone acetylation but also include aberrant DNA gene methylation hence, the combination of an HDAC inhibitor with a DNA demethylating agent is a promising therapy to be tested. Here we report a mycosis fungoides patient having a dramatic response to hydralazine and valproate, two repositioned drugs as HDAC and DNA methylation inhibitors, respectively.
Mass Fingerprinting of the Venom and Transcriptome of Venom Gland of Scorpion Centruroides tecomanus
Laura L. Valdez-Velázquez, Verónica Quintero-Hernández, Maria Teresa Romero-Gutiérrez, Fredy I. V. Coronas, Lourival D. Possani
PLOS ONE , 2013, DOI: 10.1371/journal.pone.0066486
Abstract: Centruroides tecomanus is a Mexican scorpion endemic of the State of Colima, that causes human fatalities. This communication describes a proteome analysis obtained from milked venom and a transcriptome analysis from a cDNA library constructed from two pairs of venom glands of this scorpion. High perfomance liquid chromatography separation of soluble venom produced 80 fractions, from which at least 104 individual components were identified by mass spectrometry analysis, showing to contain molecular masses from 259 to 44,392 Da. Most of these components are within the expected molecular masses for Na+- and K+-channel specific toxic peptides, supporting the clinical findings of intoxication, when humans are stung by this scorpion. From the cDNA library 162 clones were randomly chosen, from which 130 sequences of good quality were identified and were clustered in 28 contigs containing, each, two or more expressed sequence tags (EST) and 49 singlets with only one EST. Deduced amino acid sequence analysis from 53% of the total ESTs showed that 81% (24 sequences) are similar to known toxic peptides that affect Na+-channel activity, and 19% (7 unique sequences) are similar to K+-channel especific toxins. Out of the 31 sequences, at least 8 peptides were confirmed by direct Edman degradation, using components isolated directly from the venom. The remaining 19%, 4%, 4%, 15% and 5% of the ESTs correspond respectively to proteins involved in cellular processes, antimicrobial peptides, venom components, proteins without defined function and sequences without similarity in databases. Among the cloned genes are those similar to metalloproteinases.
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