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Search Results: 1 - 10 of 89868 matches for " Maria Teresa Moreno-Flores "
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The 2nd Step by Step International Spinal Cord Repair—Combining research Step by Step into multi-pronged approaches for spinal cord repair  [PDF]
Maria Teresa Moreno-Flores, Francois Ferón, Victor Arvanian, Armin Blesch, Armin Curt, David J. Fink, Marina Mata, Kinichi Nakashima, Xavier Navarro, Francisco Javier Rodríguez, Michal Schwartz, Mikael Svensson, Erik Sundstrom, Joan Romero, Filip Lim
Journal of Biomedical Science and Engineering (JBiSE) , 2013, DOI: 10.4236/jbise.2013.67A2003
Abstract: On April 26-27, 2013, the Step by Step Foundation hosted the Second International Spinal Cord Repair Meeting at the Fira Barcelona Convention Center in Hospitalet de Llobregat, Spain, highlighting some of the exciting research including clinical trials which show promise for treatments for this devastating disorder. This meeting brought together clinicians, clinical scientists and molecular biologists from more than 10 countries to evaluate current knowledge on clinical, cellular, and biomolecular aspects of spinal cord injury. A major goal of the conference in advancing the translation of research data to the clinic was to promote multi-pronged approaches for therapy of this complex problem.
Olfactory Ensheathing Glia: Drivers of Axonal Regeneration in the Central Nervous System?
M. Teresa Moreno-Flores,Javier Díaz-Nido,Francisco Wandosell,Jesús Avila
Journal of Biomedicine and Biotechnology , 2002, DOI: 10.1155/s1110724302000372
Abstract: Olfactory ensheathing glia (OEG) accompany olfactory growing axons in their entry to the adult mammalian central nervous system (CNS). Due to this special characteristic, considerable attention has been focused on the possibility of using OEG for CNS regeneration. OEG present a large heterogeneity in culture with respect to their cellular morphology and expressed molecules. The specific characteristics of OEG responsible for their regenerative properties have to be defined. These properties probably result from the combination of several factors: molecular composition of the membrane (expressing adhesion molecules as PSA-NCAM, L1 and/or others) combined with their ability to reduce glial scarring and to accompany new growing axons into the host CNS. Their capacity to produce some neurotrophic factors might also account for their ability to produce CNS regeneration.
Olfactory Ensheathing Glia: Drivers of Axonal Regeneration in the Central Nervous System?
Moreno-Flores M. Teresa,Díaz-Nido Javier,Wandosell Francisco,Avila Jesús
Journal of Biomedicine and Biotechnology , 2002,
Abstract: Olfactory ensheathing glia (OEG) accompany olfactory growing axons in their entry to the adult mammalian central nervous system (CNS). Due to this special characteristic, considerable attention has been focused on the possibility of using OEG for CNS regeneration. OEG present a large heterogeneity in culture with respect to their cellular morphology and expressed molecules. The specific characteristics of OEG responsible for their regenerative properties have to be defined. These properties probably result from the combination of several factors: molecular composition of the membrane (expressing adhesion molecules as PSA-NCAM, L1 and/or others) combined with their ability to reduce glial scarring and to accompany new growing axons into the host CNS. Their capacity to produce some neurotrophic factors might also account for their ability to produce CNS regeneration.
Stress relaxation microscopy (STREM): Imaging mechanical force decay in cells
Susana Moreno-Flores,Rafael Benitez,Maria dM Vivanco,Jose Luis Toca-Herrera
Physics , 2009,
Abstract: We have developed a novel scanning probe-based methodology to study cell biomechanics. The time dependence of the force exerted by the cell surface on a scanning probe at constant local deformation has been used to extract local relaxational responses. The generalized Maxwell viscoelastic model that accounts for multi relaxations fully describes the mechanical behaviour of the cell surface that exhibits a bimodal relaxation. Within the range of tested forces (0.1-4 nN) a slow and a fast relaxation with characteristic times of 0.1 and 1s have been detected and assigned to rearrangements in the cell membrane and cytoskeleton cortex, respectively. Relaxation time mapping allows to simultaneously detect non-uniformities in membrane and cytoskeletal mechanical behaviour and can be used as both identifying and diagnosing tools for cell type and cell disease.
Stress relaxation and creep experiments with the atomic force microscope: a unified method to calculate elastic moduli and viscosities of biomaterials (and cells)
Susana Moreno-Flores,Rafael Benitez,Maria dM Vivanco,Jose Luis Toca-Herrera
Quantitative Biology , 2010, DOI: 10.1088/0957-4484/21/44/445101
Abstract: We show that the atomic force microscope can perform stress relaxation and creep compliance measurements on living cells. We propose a method to obtain the mechanical properties of the studied biomaterial: the relaxation time, the elastic moduli and the viscosity.
On the lipid-bacterial protein interaction studied by quartz crystal microbalance with dissipation, transmission electron microscopy and atomic force microscopy
Mihaela Delcea,Susana Moreno-Flores,Dietmar Pum,Uwe Bernd Sleytr,Jose Luis Toca-Herrera
Physics , 2009,
Abstract: The interaction between the bacterial S-protein SbpA on different types of lipid membranes has been studied using atomic force microscopy, transmission electron microscopy, and quartz crystal microbalance with dissipation. On one hand, It has been found that the bacterial forms two dimensional nanocrystals on zwitterionic DOPC bilayers and negatively charged DMPG vesicles adsorbed on mica, on zwitterionic DPPC and charged DPPC/DMPG (1:1) monolayers adsorbed on carbon grids. On the other hand, SbpA protein adsorption took place on zwitterionic DOPC bilayers and DOPC/DOPS (4:1) bilayers, previously adsorbed on silicon supports. SbpA adsorption also took place on DPPC/DOPS (1:1) monolayers adsorbed on carbon grids. Finally, neither SbpA adsorption, nor recrystallization was observed on zwitterionic DMPC vesicles (previously adsorbed on polyelectrolyte multilayers), and on DPPC vesicles supported on silicon.
Geometric capture and escape of a microswimmer colliding with an obstacle
Saverio E. Spagnolie,Gregorio R. Moreno-Flores,Denis Bartolo,Eric Lauga
Physics , 2014, DOI: 10.1039/C4SM02785J
Abstract: Motivated by recent experiments, we consider the hydrodynamic capture of a microswimmer near a stationary spherical obstacle. Simulations of model equations show that a swimmer approaching a small spherical colloid is simply scattered. In contrast, when the colloid is larger than a critical size it acts as a passive trap: the swimmer is hydrodynamically captured along closed trajectories and endlessly orbits around the colloidal sphere. In order to gain physical insight into this hydrodynamic scattering problem, we address it analytically. We provide expressions for the critical trapping radius, the depth of the "basin of attraction," and the scattering angle, which show excellent agreement with our numerical findings. We also demonstrate and rationalize the strong impact of swimming-flow symmetries on the trapping efficiency. Finally, we give the swimmer an opportunity to escape the colloidal traps by considering the effects of Brownian, or active, diffusion. We show that in some cases the trapping time is governed by an Ornstein-Uhlenbeck process, which results in a trapping time distribution that is well-approximated as inverse-Gaussian. The predictions again compare very favorably with the numerical simulations. We envision applications of the theory to bioremediation, microorganism sorting techniques, and the study of bacterial populations in heterogeneous or porous environments.
Effect of Growth Habit on Agronomic Characters in Faba Bean
Salvador Nadal,Alfonso Cabello,Fernando Flores,Maria Teresa Moreno
Agriculturae Conspectus Scientificus (ACS) , 2005,
Abstract: A change in growth habit in order to concentrate the harvest of green pods at the same date will allow for mechanical harvesting of both green pods and seeds. The ti (terminal inflorescence) gene was incorporated into horticultural major type genotypes by backcrossing. These new lines show shorter periods of both flowering and maturity, allowing for an early production of green pods. The dry seed yield observed is greater for the best indeterminate cultivars than those of determinate habit.
Optimal Population Density on Determinate Growth Habit Faba Bean for Immature Green Pod Production
Salvador Nadal,Maria Teresa Moreno
Agriculturae Conspectus Scientificus (ACS) , 2006,
Abstract: The effects of population density on immature green pod yield were studied during two seasons in southern Spain. The plants were grown at three densities (11, 17 and 33 plants/m2). Immature green pod yields generally increased as population density increased. The optimum density for these varieties is 33 plants/m2.
Engraftment of pre-differentiated stem cells into cardiomyocytes in an animal model of ischemic cardiopathy  [PDF]
Maria Teresa Gonzalez-Garza, Citlali Alcaraz, Lizbeth Gonzalez-Jara, Demetrio Arcos, Jorge E. Moreno Cuevas
Stem Cell Discovery (SCD) , 2014, DOI: 10.4236/scd.2014.41001
Abstract:

Stem cell therapy for cardiac infarct regeneration has been widely used in clinical research. Despite the fact that important advances in this field have been reached, the observed recovery does not demonstrate new cardiac muscle formation. Benefits have been observed due to an improvement in neovascularisation. The main objective of this study was to determine if pre-differentiated stem cells into cells of myocardic lineage are capable of engraftment in animal models with induced cardiac infarct and are capable of truly differentiating into myocardiocytes. Bone marrow rat stem cells were pre-differentiated with 5-AZ. After 4 weeks, pre-differentiated stem cells express muscarinic 1, 2 and β adrenergic 2 receptors. Also, proteins such as sarcomeric α-actin, cardiac myosin heavy chain, desmin and vimentin were detected by immunocytochemistry. Cells were transplanted intracardialy in an ischemic cardiac rat model. Pre-differentiated or non differentiated cells were transplanted after 4 weeks post infarct induction. Histopathology of the hearts was made 2 weeks after cell transplantation. Typical granulated tissue, scare formation and neovascularisation were observed in both groups. However, in those hearts from rats inoculated with pre-differentiated cells many appeared atypical and were α-actin sarcomeric positive. These events suggest that pre-differentiated cells conserve some muscle characteristic traits in situ that at least last for two weeks after transplantation.

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