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Search Results: 1 - 10 of 273480 matches for " Maria Helena P. Fungaro "
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Phylogenetic position of an uncharacterized Brazilian strain of bovine papillomavirus in the genus Xipapillomavirus based on sequencing of the L1 open reading frame
Lunardi, Michele;Claus, Marlise P.;Alfieri, Amauri A.;Fungaro, Maria Helena P.;Alfieri, Alice F.;
Genetics and Molecular Biology , 2010, DOI: 10.1590/S1415-47572010005000091
Abstract: the use of pcr assays with degenerate primers has suggested the existence of numerous as yet uncharacterized bovine papillomaviruses (bpv). despite the endemic nature of bpv infections, the identification of bpv types in brazilian cattle is still only sporadic. however, in a recent analysis of a partial segment of the l1 gene, we observed notable diversity among the bpv types detected. the aim of this study was to determine the phylogenetic position of the previously identified wild strain bpv/br-uel2 detected in the state of paraná in brazil. since previous analysis of the partial l1 sequence had shown that this strain was most closely related to bpv type 4, genus-specific primers were designed. phylogenetic analysis using complete l1 orf sequences revealed that bpv/br-uel2 was related to bpv types classified in the genus xipapillomavirus and shared the highest l1 nucleotide sequence similarity with bpv type 4 (78%). this finding suggests that bpv/br-uel2 should be classified as a potential new type of bpv in the genus xipapillomavirus.
Identification of the recently described new type of bovine papillomavirus (BPV-8) in a Brazilian beef cattle herd
Claus, Marlise P.;Lunardi, Michele;Alfieri, Alice F.;Sartori, Daniele;Fungaro, Maria Helena P.;Alfieri, Amauri A.;
Pesquisa Veterinária Brasileira , 2009, DOI: 10.1590/S0100-736X2009000100003
Abstract: bovine papillomavirus type 8 (bpv-8) was first detected and described in teat warts as well as in healthy teat skin from cattle raised in japan. the entire viral genome was sequenced in 2007. additionally, a variant of bpv-8, bpv-8-eb, was also identified from papillomatous lesions of a european bison in slovakia. in brazil, despite the relatively common occurrence of bpv infections, the identification and determination of viral types present in cattle is still sporadic. the aim of this study is to report the occurrence of the recently described bpv-8 in brazil. the virus was identified in a skin warts obtained from a beef cattle herd located in parana state, southern brazil. the papilloma had a macular, non-verrucous gross aspect and was located on the dorsal thorax of a cow. polymerase chain reaction (pcr) was performed using generic primers for partial amplification of l1 gene. the obtained amplicon (480bp) was cloned and two selected clones were sequenced. the nucleotide sequence was compared to existing papillomaviral genomic sequences, identifying the virus as bpv type 8. this study represents the first report of bpv-8 occurrence in brazil, what suggests its presence among brazilian cattle.
Identification of fungi of the genus Aspergillus section nigri using polyphasic taxonomy
Silva, Daiani M.;Batista, Luís R.;Rezende, Elisangela F.;Fungaro, Maria Helena P.;Sartori, Daniele;Alves, Eduardo;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000200044
Abstract: in spite of the taxonomy of the aspergillus species of the nigri section being regarded as troublesome, a number of methods have been proposed to aid in the classification of this section. this work aimed to distinguish aspergillus species of the nigri section from foods, grains and caves on the basis in polyphasic taxonomy by utilizing morphologic and physiologic characters, and sequencing of ?-tubulin and calmodulin genes. the morphologic identification proved useful for some species, such as a. carbonarius and aspergillus sp ufla dca 01, despite not having been totally effective in elucidating species related to a. niger. the isolation of the species of the nigri section on creatine sucrose agar (crea) enabled to distinguish the aspergillus sp species, which was characterized by the lack of sporulation and by the production of sclerotia. scanning electron microscopy (sem) allowed distinguishing the species into two distinct groups. the production of ochratoxin a (ota) was only found in the a. carbonarius and a. niger species. the sequencing of β-tubulin gene was efficient in differing most of the aspergillus species from the nigri section with the exception of aspergillus ufla dca 01, which could not be distinguished from a. costaricaensis. this species is morphologically similar to a. costaricaencis for its low sporulation capacity and high sclerotia production, but it differs morphologically from a. costaricaensis for its conidial ornamentation and size of vesicles. equally, based on partial calmodulin gene sequence data aspergillus ufla dca 01 differs from a. costaricaensis.
Structural characterization of the bglH gene encoding a beta-glucosidase-like enzyme in an endophytic Bacillus pumilus strain
Bogas, Andréa C.;Watanabe, Maria Angelica E.;Barbosa, Aneli;Vilas-Boas, Laurival A.;Bonatto, Ana C.;Dekker, Robert;Souza, Emanuel M.;Fungaro, Maria Helena P.;
Genetics and Molecular Biology , 2007, DOI: 10.1590/S1415-47572007000100018
Abstract: a beta-glucosidase-like enzyme-encoding gene (bglh) of an endophytic bacillus pumilus strain (cl16) was cloned using a shotgun genomic library constructed in escherichia coli. the nucleotide sequence of the entire cloned fragment (2484 bp) was determined and characterized. an incomplete open reading frame (orf) of 534 bp (orf1) designated bglp and a complete orf of 1419 bp (orf2) designated bglh, located in the fragment, are organized in an operon. the protein deduced from 1419 bp (orf2) had 472 amino acid residues without a characteristic signal peptide sequence, suggesting that the enzyme is localized in the cytoplasm. the amino acid sequence deduced from bglh gene had high similarity with b-glucosidases from the glycosyl hydrolase family 1. over-expression of the b. pumilus bglh gene in e. coli showed a 54 kda protein whose identity was confirmed by mass spectrometry (maldi-tof).
Aspergillus bertholletius sp. nov. from Brazil Nuts
Marta H. Taniwaki, John I. Pitt, Beatriz T. Iamanaka, Daniele Sartori, Marina V. Copetti, Arun Balajee, Maria Helena P. Fungaro, Jens C. Frisvad
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0042480
Abstract: During a study on the mycobiota of brazil nuts (Bertholletia excelsa) in Brazil, a new Aspergillus species, A. bertholletius, was found, and is described here. A polyphasic approach was applied using morphological characters, extrolite data as well as partial β-tubulin, calmodulin and ITS sequences to characterize this taxon. A. bertholletius is represented by nineteen isolates from samples of brazil nuts at various stages of production and soil close to Bertholletia excelsa trees. The following extrolites were produced by this species: aflavinin, cyclopiazonic acid, kojic acid, tenuazonic acid and ustilaginoidin C. Phylogenetic analysis using partial β-tubulin and camodulin gene sequences showed that A. bertholletius represents a new phylogenetic clade in Aspergillus section Flavi. The type strain of A. bertholletius is CCT 7615 ( = ITAL 270/06 = IBT 29228).
An overview on molecular markers for detection of ochratoxigenic fungi in coffee neans
Fungaro, Maria Helena Pelegrinelli;Sartori, Daniele;
Brazilian Archives of Biology and Technology , 2009, DOI: 10.1590/S1516-89132009000700001
Abstract: ochratoxin a (ota) is one of the most abundant food-contaminating mycotoxins. its occurrence in several agricultural commodities has been considered a worldwide problem. this toxin is mainly produced by aspergillus species. ota has nephrotoxic, immunosuppressive, and carcinogenic effects and consequently the contamination with this toxin represents a high risk for human and animal health. in the last 5 years, several investigators have applied molecular methods in order to develop pcr assays for identifying and quantifying ota-producing fungi in coffee beans samples. the main objective is to allow the detection of microorganisms capable of producing ota, preferentially prior to ochratoxin production and accumulation. in this contribution several of these attempts will be reviewed and discussed.
Transgênicos e evolu??o dirigida
Azevedo, Jo?o Lúcio de;Fungaro, Maria Helena Pelegrinelli;Vieira, Maria Lúcia Carneiro;
História, Ciências, Saúde-Manguinhos , 2000, DOI: 10.1590/S0104-59702000000300014
Abstract: mutation events are responsible for the generation of genetic variability in the populations enabling the occurrence of natural selection which favors the better-adapted types. the exploitation of this variability, though carried out empirically, dates from ten thousand years ago with the domestication of the first cultivated crops. with the advent of genetics, rational selection procedures were adopted with a view to the genetic breeding of plants, animals and microorganisms which might be of interest to men. recently, new dna manipulation techniques came up enabling the transference of genes between organisms, cutting across barriers which hindered crossings between the vegetable, animal, protist and fungus kingdoms. the generation of genetically modified organisms, or transgenics, has aroused a heated and controversial debate in various sectors of our society. yet we must be cautious before generalizing the use of transgenics since each one should be analyzed at a time for its particular advantages and drawbacks, and for its contribution to the improvement of life quality. this paper also considers recent methods of mutation and in vitro genic recombination.
Genetic instability of sugarcane plants derived from meristem cultures
Zucchi Maria Imaculada,Arizono Hideto,Morais Vicente Alberto,Fungaro Maria Helena Pelegrinelli
Genetics and Molecular Biology , 2002,
Abstract: The RADP (Random amplified polymorphic DNA) technique was used to detect tissue-culture-induced variations in sugarcane. Plants of the Brazilian variety RB83-5486 propagated via rhizomes and via meristem cultures were studied. The polymorphism rate for 98 RAPD loci was 6.93% when the plants derived from meristems. Besides, in order to evaluate the influence of the number of subcultures on the generation of somaclonal variation, field-grown RB83-5486 plants derived from 10 meristems were studied after five subcultivations. Although different rates of polymorphism were observed, there was no direct association with the stage of subcultivation. The analysis of plants of two sugarcane varieties cultivated in vitro from meristems showed that variety RB83-5486 was more unstable than variety SP80-185.
Transgênicos e evolu o dirigida
Azevedo Jo?o Lúcio de,Fungaro Maria Helena Pelegrinelli,Vieira Maria Lúcia Carneiro
História, Ciências, Saúde-Manguinhos , 2000,
Abstract: Os eventos de muta o s o responsáveis pela gera o de variabilidade genética nas popula es, permitindo a a o da sele o natural que favorece os tipos mais adaptados. A explora o dessa variabilidade, embora de forma empírica, teve início há cerca de dez mil anos, quando se deu a domestica o das primeiras culturas agrícolas. Com o advento da genética, práticas racionais de sele o passaram a ser adotadas visando o melhoramento genético de plantas, animais e microrganismos de interesse para o homem. Recentemente, surgiram as técnicas de manipula o de DNA, permitindo a transferência de genes entre organismos, transpondo barreiras de cruzamento entre os reinos vegetal, animal, protista e fungi. A gera o dos organismos geneticamente modificados, ou transgênicos, tem sido alvo de polêmica e discuss o nos diversos segmentos da sociedade. Porém, n o se deve generalizar o uso dos transgênicos, pois cada um deve ser analisado quanto às suas vantagens, desvantagens e contribui o à melhoria da qualidade de vida. Métodos recentes de muta o e recombina o gênica in vitro s o também apresentados no texto.
Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting
Ono, Elisabete Yurie Sataque;Fungaro, Maria Helena Pelegrinelli;Sofia, Silvia Helena;Miguel, Tatiana de ávila;Sugiura, Yoshitsugu;Hirooka, Elisa Yoko;
Brazilian Archives of Biology and Technology , 2010, DOI: 10.1590/S1516-89132010000400026
Abstract: in this study a total of 16 fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (fb) production and random amplified polymorphic dna (rapd). all the strains produced fb1 and fb2 with levels ranging from 2.41 to 3996.36 μg/g, and from 1.18 to 1209.91 μg/g, respectively. from the 16 f. verticillioides strains, four were identified as low (3.59 to 1289.84 μg/g), eight as intermediate (>1289.84 to 3772.44 μg/g) and four strains as high (>3772.44 μg/g) fumonisin producers. from the total of 105 loci amplified, 60 (57.14%) were polymorphic. rapd analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. although rapd markers were capable of discriminating the different f. verticillioides strains, there was no clear association between these makers and fumonisin production.
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