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Search Results: 1 - 10 of 325895 matches for " Maria E. Marin-Casta?o "
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Cigarette Smoke-Related Hydroquinone Dysregulates MCP-1, VEGF and PEDF Expression in Retinal Pigment Epithelium in Vitro and in Vivo
Marianne Pons,Maria E. Marin-Castao
PLOS ONE , 2012, DOI: 10.1371/journal.pone.0016722
Abstract: Age-related macular degeneration (AMD) is the leading cause of legal blindness in the elderly population. Debris (termed drusen) below the retinal pigment epithelium (RPE) have been recognized as a risk factor for dry AMD and its progression to wet AMD, which is characterized by choroidal neovascularization (CNV). The underlying mechanism of how drusen might elicit CNV remains undefined. Cigarette smoking, oxidative damage to the RPE and inflammation are postulated to be involved in the pathophysiology of the disease. To better understand the cellular mechanism(s) linking oxidative stress and inflammation to AMD, we examined the expression of pro-inflammatory monocyte chemoattractant protein-1 (MCP-1), pro-angiogenic vascular endothelial growth factor (VEGF) and anti-angiogenic pigment epithelial derived factor (PEDF) in RPE from smoker patients with AMD. We also evaluated the effects of hydroquinone (HQ), a major pro-oxidant in cigarette smoke on MCP-1, VEGF and PEDF expression in cultured ARPE-19 cells and RPE/choroids from C57BL/6 mice.
Giardiasis in children living in post-earthquake camps from Armenia (Colombia)
Fabiana Lora-Suarez, Carolina Marin-Vasquez, Nelsy Loango, Martha Gallego, Elizabeth Torres, Maria Gonzalez, Jhon Castao-Osorio, Jorge Gómez-Marín
BMC Public Health , 2002, DOI: 10.1186/1471-2458-2-5
Abstract: Fecal samples were obtained from 217 children aged between 3 and 13 years. Stool samples were studied by direct wet examination and stained with ferric hematoxilin for microscopical examination. Epidemiological data were collected by questionnaire and analyzed by using the Epi-info software (CDC, Atlanta 2001).Giardia cysts were observed in 60.4% of the samples presented and trophozoites in 4.6%. The following epidemiological and laboratory factors were significantly associated with Giardia infection: 1. Use of communal toilet (vs. individual toilet) OR: 3.9, CI95%: 1.2–16; 2. water provision by municipal ducts (vs. water provision by individual tanks) OR: 3.5, CI95% 1.1–14, and 3. presence of mucus in stool OR: 2.3, IC95%: 0.9–6.7.A high prevalence of giardiasis was found in children living in temporary houses after the 1999 earthquake in Armenia (Colombia). Giardiasis is an emerging disease in post-disaster situations and adequate prevention measures should be implemented during these circumstances.On the 25 January, 1999 an earthquake with an intensity of 6.2 points on the Richter's scale occurred in a focus located only 20 kilometers away from Armenia, Colombia. Although 28 towns or municipalities, including Armenia, were severely damaged by the earthquake, the worst effects occurred in Armenia itself and in its surrounding towns. The consequences of the catastrophe were devastating, mainly because of the loss of people's property and belongings: around 70% of Armenian houses were destroyed and in some neighboring towns the percentage was greater than 80%. In the rural area, the proportion of lost houses was also 80%. The death toll was estimated at 1184, and the number of people injured was more than 5,000 [1].The region known as "Departmento del Quindío" includes, according to the political and administrative division of the Colombian territory, several small towns and its capital city, Armenia. The whole area of influence of the telluric movement is known as
METODOLOGIAS PARA EVALUAR IN VITRO LA ACTIVIDAD ANTIBACTERIANA DE COMPUESTOS DE ORIGEN VEGETAL
Luz Stella Ramirez,Darwin Marin Castao
Scientia Et Technica , 2009,
Abstract: Los productos naturales, proveen oportunidades ilimitadas para el hallazgo de nuevos fármacos. En esta revisión se presentan algunas metodologías utilizadas para la evaluación de la actividad antibacteriana de compuestos de origen vegetal.
Hacerse entender: la experiencia de pacientes sometidos a ventilación mecánica sin efectos de sedación
Henao Castao,ángela Maria;
Investigación y Educación en Enfermería , 2008,
Abstract: the mechanical ventilation mechanism is a support method used to safeguard the life of people in a critical state. traditionally, it has been used in patients under the effects of continuous sedation, which implies a total abstraction of their current situation for the patients; such scenarios afford the patients a traumatic one to wake up, broadly informed in the literature, for the adverse effects of the sedative medications, like the psychological implications that it can cause independent of quality of care. objective: to describe the different communication forms used for patients whose experience in an intensive critical care unit has involved the mechanical ventilation without continues sedation effects. methodology: the investigation was carried out with phenomenological focus during 2006 and had the participation of seven men and two women with ages between 24 and 75 years of age. the technique of information gathering was the in-depth interview. results: the description of each experience allowed understanding of the human being critically sick with an artificial air way as living medium, requesting active ways to be made understood. conclusion: it demonstrated the importance for the patient to be treated as an active subject by means of the communication with the health personnel and relatives. many non verbal tools were employed and their contribution brought upon a positive perception of the experience.
Hacerse entender: la experiencia de pacientes sometidos a ventilación mecánica sin efectos de sedación Make yourself clear: experience for submitted patients to mechanical ventilation without sedation side effects
ángela Maria Henao Castao
Investigación y Educación en Enfermería , 2008,
Abstract: La ventilación mecánica es un método de soporte de la vida de personas en estado crítico. Tradicionalmente se ha empleado en pacientes bajo el efecto de sedación continua lo que significa para ellos una total abstracción de su situación actual. Tal práctica depara un traumático despertar, ampliamente informado en la literatura, tanto por los efectos adversos de los medicamentos sedantes, como por las implicaciones sicológicas de haber sido objeto y no sujeto de cuidado. Este trabajo, por el contrario, está enfocado en pacientes con ventilación mecánica, sin sedación. Objetivo: describir las diferentes formas de comunicación empleadas por pacientes cuya experiencia en una Unidad de Cuidado Intensivo ha sido la ventilación mecánica sin efectos de sedación continúa. Metodología: se realizó una investigación con enfoque fenomenológico, durante el 2006, que contó con la participación de siete hombres y dos mujeres con edades entre 24 y 75 a os de edad. La técnica de recolección de la información fue la entrevista en profundidad. Resultados: la descripción de cada experiencia permitió entender cómo el ser humano, estando críticamente enfermo, con una vía aérea artificial como medio de vida, activa un importante número de formas para hacerse entender. Conclusión: se demostró la importancia que tiene para el paciente ser tratado como sujeto activo en su experiencia de comunicación con el personal de salud y sus familiares, dado que fue significativa la multiplicidad de herramientas no verbales empleadas que contribuyeron a una percepción positiva de la experiencia. The mechanical ventilation mechanism is a support method used to safeguard the life of people in a critical state. Traditionally, it has been used in patients under the effects of continuous sedation, which implies a total abstraction of their current situation for the patients; such scenarios afford the patients a traumatic one to wake up, broadly informed in the literature, for the adverse effects of the sedative medications, like the psychological implications that it can cause independent of quality of care. Objective: to describe the different communication forms used for patients whose experience in an intensive critical care unit has involved the mechanical ventilation without continues sedation effects. Methodology: the investigation was carried out with phenomenological focus during 2006 and had the participation of seven men and two women with ages between 24 and 75 years of age. The technique of information gathering was the in-depth interview. Results: The description of each experience a
The confined two-dimensional hydrogen atom in the linear variational approach
N. Aquino,E. Castao
Revista mexicana de física , 2005,
Abstract: En este trabajo analizamos el problema de un átomo de hidrógeno bidimensional confinado al interior de una región circular de paredes impenetrables. Estudiamos los efectos de tama o sobre los valores propios de la energía como función del radio del círculo. Usamos el método variacional lineal con la base de la partícula libre en un círculo. Comparamos nuestros resultados con los obtenidos mediante la teoría de perturbaciones y el método Rayleigh-Ritz que habían sido reportados previamente.
Searching for pairing energies in phase space
M. Calixto,O. Casta?os,E. Romera
Physics , 2014, DOI: 10.1209/0295-5075/108/47001
Abstract: We obtain a representation of pairing energies in phase space, for the Lipkin-Meshkov-Glick and general boson Bardeen-Cooper-Schrieffer pairing models. This is done by means of a probability distribution of the quantum state in phase space. In fact, we prove a correspondence between the points at which this probability distribution vanishes and the pairing energies. In principle, the vanishing of this probability distribution is experimentally accessible and additionally gives a method to visualize pairing energies across the model control parameter space. This result opens new ways to experimentally approach quantum pairing systems.
Velocity selector with a microwave magnetic dipole transition
L. O. Casta?os,E. Gomez
Physics , 2014, DOI: 10.1103/PhysRevA.89.013406
Abstract: We proposed a method based on microwave magnetic dipole transitions to prepare samples of atoms with well defined position and velocity. Each microwave pulse corresponds to a position measurement for the atoms and two pulses separated by a given delay result in a velocity measurement. The method gives velocity sensitivity approaching that obtained with Raman transitions but it is easier to implement. Moreover, it has the advantages that it also selects in position and has less demanding experimental requirements. The method can be demonstrated in a magneto-optical trap.
INMUNOGENICIDAD DE LA PROTEíNA RECOMBINANTE ASP1R DE Ancylostoma caninum EN UN MODELO MURINO
Maria Giraldo G,Jhon C Castao O
Revista MVZ Córdoba , 2009,
Abstract: Objetivo. Construir un plásmido recombinante que exprese la proteína ASP1r de Ancylostoma caninum y evaluar su capacidad inmunogénica en un modelo murino. Materiales y métodos. Se realizó extracción de ARN de parásitos adultos de Ancylostoma caninum, se amplificó por RT –PCR el gen de la proteína ASP1. Este gen fue insertado en el vector pcDNA3. El inserto fue digerido con Bamh1 y EcoR1 y clonado direccionalmente. Posteriormente, se llevó a cabo transformación y selección de las células de E. coli DH5a competentes con el producto de la ligación. Se realizó un tamizaje por PCR confirmando la presencia del gen ASP1. El vector pcDNA3-ASP1 fue administrado vía intraglandular en la parotida e intramuscular en ratones Balb/c. En estos animales se les realizó determinación de anticuerpos en suero y saliva mediante las técnicas de ELISA e inmunohistoquímica. Resultados. Se determinó que el plásmido pcDNA3-ASP1 fue incorporado y expresado células E. coli DH5a. Este plásmido recombinante indujo la producción de anticuerpos Anti-ASP1 específicos en ratones Balb/c. Conclusiones. Se logró demostrar que la utilización de pcDNA3-ASP1 no produjo reacciones desfavorables en ratones Balb/c, además indujo respuesta humoral contra la proteína pcDNA3-ASP1 de excreción/secreción de Ancylostoma caninum en ratones.
Intera??o da deficiência hídrica e da toxicidade do alumínio em guandu cultivado em hidroponia
Marin, Ad?o;Santos, Durvalina Maria Mathias dos;
Pesquisa Agropecuária Brasileira , 2008, DOI: 10.1590/S0100-204X2008001000003
Abstract: the objective of this work was to evaluate the interaction effect of water stress and aluminum toxicity on the initial growth and free proline contents in two cultivars of pigeon pea, iapar 43-arat? and iac fava larga, cultivated in hydroponics. the seedlings were submitted to stresses in nutritive solution (ph 3.8), osmotic potentials 0, -0.004, -0.006, -0.008 and -0.010 mpa, with 0, 0.25, 0.50, 0.75 and 1 mmol dm-3 al3+ . the experiment was carried out in a plant growth room, under a luminary unit of average irradiance 190 mmol m-2 s-1 , 12-hour photoperiod and 25+1oc temperature. a completely randomized experimental design was used in factorial arrangement 2x5x5 (two pigeon pea cultivars, five osmotic potential and five levels), with four replicates. data were submitted to analyses of polynomial regression, cluster and main components. water stress causes growth reduction of pigeon pea aerial part, whereas aluminum toxicity causes radicular growth reduction. there was increase of free proline contents in the two cultivars under water stress, and only in iac fava larga under aluminum toxicity. multivariate analysis showed high correlation in growth and accumulation of proline for cultivar iac fava larga, evidencing probable tolerance to associated stresses.
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