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Search Results: 1 - 10 of 107877 matches for " Maria Alice Z Coelho "
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Study of microbial flora aclimatization to increasing concentrations of efluents “in natura” of the industry of fishing in reactors of sequential batch
Roberta R. Ribeiro,Daniela P. Mesquita,Maria Alice Z. Coelho
Engenharia Ambiental : Pesquisa e Tecnologia , 2009,
Abstract: The present work analyzes the adaptation and activity of a sludge belonging to a UASB reactor of the wastewater treatment unit from a fish processing industry, in a bench scale Sequencing Batch Reactor for biological nitrogen removal. The sludge adaptation was carried using an increasing sequence of the applied load from 1:3.33; 1:2.5; 1:2.0; regarding to volume of in natura wastewater : synthetic sewage. The initial inocullum concentration employed was 20 g VSS / L. During this period COD, nitrate and ammonia concentrations were followed and a removal about 80% of the organic matter and up to 97% of nitrogen could be achieved. An experiment employing the greater dilution (1:3.33) with 0.5% salt (as NaCl) was also carried to evaluate the salinity effect which is considered in the analysis of the results herein presented.
Optimización de la Concentración de L-Cisteína para la producción de 1,3-Propanodiol por una vía Biotecnológica
Ferreira,Tatiana F; Ribeiro,Roberta dos R; Matos,Pedro M; Coelho,Maria Alice Z;
Información tecnológica , 2013, DOI: 10.4067/S0718-07642013000100006
Abstract: glycerol was converted into 1,3-propanediol (1,3-pdo) using citrobacter freundii atcc 8090. 1,3-pdo is a bifunctional molecule that can be used in many applications, being of great commercial interest. the most recent route used for the production of this compound is through biotechnology using some micro-organisms capable of anaerobically converting glycerol into 1,3-pdo. the conversion done makes it necessary the addition of cysteine ??to the culture medium. five experiments were performed with cysteine at five concentrations to verify which is the optimum concentration that does not affect conversion of glycerol into 1,3-pdo. the concentrations used were 0.05, 0.10, 0.15, 0.20 and 0.25 gl-1. the yields were 15, 20, 26, 35 and 28 % respectively. according to the results it is concluded that the optimum cysteine concentration ??for 1,3-pdo production is 0.20 g.l-1.
Optimización de la Concentración de L-Cisteína para la producción de 1,3-Propanodiol por una vía Biotecnológica Optimization of L-Cysteine Concentration to produce 1,3-Propanediol by a Biotechnological route
Tatiana F Ferreira,Roberta dos R Ribeiro,Pedro M Matos,Maria Alice Z Coelho
Información Tecnológica , 2013,
Abstract: Se ha realizado la conversión de glicerol en 1,3-propanodiol (1,3-PDO) usando la cepa Citrobacter freundii ATCC 8090. El 1,3-PDO es una molécula bifuncional que puede ser utilizada para muchas aplicaciones, siendo así de gran interés comercial. Una ruta más reciente para la producción de este compuesto es la vía biotecnológica utilizando algunos microorganismos capaces de convertir anaeróbicamente glicerol en 1,3-PDO. La conversión realizada hace necesaria la adición de cisteína al medio de cultivo. Se realizaron cinco experimentos con cinco concentraciones de cisteína con el fin de verificar cuál es la menor concentración posible que no afecta la conversión de glicerol en 1,3-PDO. Las concentraciones utilizadas fueron 0,05; 0,10; 0,15; 0,20 y 0,25 g.L-1. Los rendimientos fueron 15, 20, 26, 35 y 28 % respectivamente. De acuerdo a los resultados se concluye que la concentración óptima de cisteína para la producción de 1,3-PDO es 0,20 g.L-1. Glycerol was converted into 1,3-propanediol (1,3-PDO) using Citrobacter freundii ATCC 8090. 1,3-PDO is a bifunctional molecule that can be used in many applications, being of great commercial interest. The most recent route used for the production of this compound is through biotechnology using some micro-organisms capable of anaerobically converting glycerol into 1,3-PDO. The conversion done makes it necessary the addition of cysteine to the culture medium. Five experiments were performed with cysteine at five concentrations to verify which is the optimum concentration that does not affect conversion of glycerol into 1,3-PDO. The concentrations used were 0.05, 0.10, 0.15, 0.20 and 0.25 gL-1. The yields were 15, 20, 26, 35 and 28 % respectively. According to the results it is concluded that the optimum cysteine concentration for 1,3-PDO production is 0.20 g.L-1.
Planejamento de enfermagem em hospital da rede pública de ensino e assistência em Goiania–Goiás
Maria Alice Coelho
Revista Eletr?nica de Enfermagem , 2008,
Abstract: O planejamento é uma importante fun o administrativa e sua execu o possibilita o alcance dos objetivos estabelecidos na organiza o. Na enfermagem, planejar e executar atividades s o imprescindíveis para garantir assistência com qualidade. A presente pesquisa trata-se de estudo descritivo, com abordagem qualitativa, realizado em um hospital de grande porte que teve como objetivo geral analisar as percep es dos gerentes quanto ao planejamento e execu o de atividades de enfermagem em hospital da rede pública de assistência e ensino de Goiania - Goiás. Participaram como sujeitos, 17 (dezessete) gerentes de enfermagem das unidades ambulatoriais e de interna o. Os dados foram coletados por meio de entrevistas semi-estruturadas e analisados conforme o que preconiza Bardin (1979). Os resultados mostraram que os gerentes elaboram o planejamento melhor do que o conceituam e que existem fatores que interferem no planejamento e execu o das atividades. Concluiu-se que os gerentes adotam o planejamento participativo, indicando mudan a de paradigma na administra o. Entre os fatores facilitadores, est o a ado o do trabalho em equipe, a proximidade entre gerência e servidores, incentivo à criatividade e interesse na execu o das a es planejadas. Os fatores dificultadores incluem a escassez de recursos humanos, materiais e financeiros; fatores relacionados aos componentes da equipe, tais como: motiva o, falta de tempo, ausência de treinamento, dificuldades em gerenciar; insuficiência de apoio da institui o e de outros servi os do hospital; estrutura física inadequada; e as intercorrências do cotidiano. A origem destes fatores foi atribuída às próprias pessoas, ao fato do hospital pertencer à rede pública, à institui o e ao sistema utilizado para o gerenciamento dos servi os de apoio.
Electrical stimulation of saccharomyces cerevisiae cultures
Araújo, Ofelia Q.F.;Coelho, Maria Alice Z.;Margarit, Isabel C.P.;Vaz-Junior, Carlos A.;Rocha-Le?o, Maria Helena M.;
Brazilian Journal of Microbiology , 2004, DOI: 10.1590/S1517-83822004000100016
Abstract: modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. in this work, the effects of applied potential on saccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. control experiments were conducted under identical conditions except for the absence of applied potential. through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. power spectral analysis was employed to sustain synchrony enhancement, and mathematical modeling was conducted for determining kinetic growth changes. monod type kinetic parameters for growth were determined by non-linear regression. the affinity constant (namely ks) presented a dependence on applied potential suggesting changes on transport across cell membrane. electrochemically promoted stress was also verified to inhibit growth as well as to induce changes on cell viability.
Análise dos registros de a es planejadas/executadas por gerentes de enfermagem de um hospital público
Maria Alice Coelho,Maria Alves Barbosa,Maria Madalena Lacerda Silva
Revista Eletr?nica de Enfermagem , 2008,
Abstract: Estudo documental, realizado em Hospital Público do Estado de Goiás que teve como objetivos identificar o direcionamento e o foco das a es dos gerentes de enfermagem das Unidades de Interna o e verificar o percentual de execu o das atividades planejadas. Os dados foram coletados em 2004 extraindo-se dos Planejamentos e Relatórios, elaborados pelos gerentes de enfermagem no período de 1999 a 2004 e organizados em um formulário. Para a análise dos dados produzidos, utilizou-se o programa Excel, vers o 2000. Embora planejadas, grande parte das atividades n o s o executadas. Entre as planejadas e executadas, destacou-se o direcionamento dado às administrativas organizacionais. Quanto ao foco, sobressaíram-se as a es de educa o continuada quando direcionadas à assistência, e as normativas quando centradas nas administrativas. Concluiu-se que o enfermeiro executa mais do que planeja. Recomenda-se a realiza o de outros estudos que possam subsidiar discuss es quanto a estes resultados em nosso meio.
Obten??o de extratos de guaraná ricos em cafeína por processo enzimático e adsor??o de taninos
Ribeiro, Bernardo Dias;Coelho, Maria Alice Zarur;Barreto, Daniel Weingart;
Brazilian Journal of Food Technology , 2012, DOI: 10.1590/S1981-67232012005000020
Abstract: guarana-flavoured beverages are very popular in brazil and have shown an excellent sales potential on foreign markets. according to brazilian law, each 100 ml of guarana-flavoured beverages must contain between 0.02 g and 0.2 g of guarana seed or its equivalent. these levels are normally obtained by adding a concentrated hydroalcoholic extract or sugar syrup containing guarana extract, directly to the beverage. however, the use of more concentrated extracts is limited by the presence of tannins, which imparts astringency and a dark colour to the final product. in this work the development of an enzymatic process to obtain non-alcoholic guarana extracts with low tannin concentrations and high caffeine contents was studied using an experimental design and adsorption processes. by way of a fractional factorial design the quantities of 0.25% (v/v) pectinase and 0.1% (v/v) glucoamylase were determined, which were maintained in the central composite design, obtaining as the optimal conditions: 0.23% (v/v) cellulase, 0.86% (v/v) hemicellulase, 1% (v/v) alpha-amylase, 5.5 h extraction time, 200 rpm and 50 °c, producing a caffeine/tannin ratio of 1.65. using a magnesium oxide adsorption process at 10% (w/v), a caffeine/tannin ratio of 7.3 was obtained.
Enzyme-Enhanced Extraction of Phenolic Compounds and Proteins from Flaxseed Meal
Bernardo Dias Ribeiro,Daniel Weingart Barreto,Maria Alice Zarur Coelho
ISRN Biotechnology , 2013, DOI: 10.5402/2013/521067
Abstract:
Obten o de extratos de guaraná ricos em cafeína por processo enzimático e adsor o de taninos Production of caffeine-rich guarana extracts using an enzymatic process and tannin adsorption
Bernardo Dias Ribeiro,Maria Alice Zarur Coelho,Daniel Weingart Barreto
Brazilian Journal of Food Technology , 2012,
Abstract: As bebidas sabor guaraná s o muito populares no Brasil e têm apresentado um excelente potencial de vendas no mercado externo. De acordo com as leis brasileiras, bebidas sabor guaraná devem conter entre 0,02 g a 0,2 g de semente de guaraná ou equivalente, para cada 100 mL de produto. Tais teores s o usualmente obtidos pela adi o de um extrato concentrado hidroalcoólico ou xarope de a úcar contendo extrato de guaraná diretamente à bebida. A utiliza o desses extratos em concentra es mais elevadas, entretanto, é limitada pela presen a dos taninos, que conferem adstringência e colora o escura ao produto final. Neste trabalho, foi estudado o desenvolvimento de um processo enzimático para obten o de extratos n o alcoólicos de guaraná, de forma a produzir um extrato contendo baixas concentra es de taninos e teores elevados de cafeína, utilizando-se planejamento experimental e processos de adsor o. Por meio de um planejamento fatorial fracionário, foram determinadas as quantidades de 0,25% (v/v) de pectinase e 0,1% (v/v) de glucoamilase, sendo mantidas no planejamento composto central, que obteve como condi es ótimas: 0,23% (v/v) de celulase, 0,86% (v/v) de hemicelulase e 1% (v/v) de alfa-amilase durante 5,5 h de extra o a 200 rpm e 50 °C, obtendo-se uma rela o cafeína/tanino de 1,65. Com o processo de adsor o com óxido de magnésio a 10% (p/v), foi alcan ada uma rela o de cafeína-tanino de 7,3. Guarana-flavoured beverages are very popular in Brazil and have shown an excellent sales potential on foreign markets. According to Brazilian law, each 100 mL of guarana-flavoured beverages must contain between 0.02 g and 0.2 g of guarana seed or its equivalent. These levels are normally obtained by adding a concentrated hydroalcoholic extract or sugar syrup containing guarana extract, directly to the beverage. However, the use of more concentrated extracts is limited by the presence of tannins, which imparts astringency and a dark colour to the final product. In this work the development of an enzymatic process to obtain non-alcoholic guarana extracts with low tannin concentrations and high caffeine contents was studied using an experimental design and adsorption processes. By way of a fractional factorial design the quantities of 0.25% (v/v) pectinase and 0.1% (v/v) glucoamylase were determined, which were maintained in the central composite design, obtaining as the optimal conditions: 0.23% (v/v) cellulase, 0.86% (v/v) hemicellulase, 1% (v/v) alpha-amylase, 5.5 h extraction time, 200 rpm and 50 °C, producing a caffeine/tannin ratio of 1.65. Using a magnesiu
Enzyme-Enhanced Extraction of Phenolic Compounds and Proteins from Flaxseed Meal
Bernardo Dias Ribeiro,Daniel Weingart Barreto,Maria Alice Zarur Coelho
ISRN Biotechnology , 2013, DOI: 10.5402/2013/521067
Abstract: Flaxseed (Linum usitatissimum) meal, the main byproduct of the flaxseed oil extraction process, is composed mainly of proteins, mucilage, and phenolic compounds. The extraction methods of phenolics either commonly employed the use of mixed solvents (dioxane/ethanol, water/acetone, water/methanol, and water/ethanol) or are done with the aid of alkaline, acid, or enzymatic hydrolysis. This work aimed at the study of optimal conditions for a clean process, using renewable solvents and enzymes, for the extraction of phenolics and proteins from flaxseed meal. After a screening of the most promising commercial preparations based on different carbohydrases and proteases, a central composite rotatable design and a mixture design were applied, achieving as optimal results a solution containing 6.6 and 152?g of phenolics and proteins, respectively. The statistical approach used in the present study for the enzyme-enhanced extraction of phenolics and proteins from the major flaxseed byproduct was effective. By means of the sequential experimental design methodology, the extraction of such compounds was increased 10-fold and 14-fold, when compared to a conventional nonenzymatic extraction. 1. Introduction Flaxseed (Linum usitatissimum) meal is the main byproduct from the flaxseed oil extraction process, being primarily used as a ruminant feed. The meal is composed of three important fractions: proteins (over 300?g?kg?1), which are rich in arginine and glutamine; amino acids that are very important in the prevention and treatment of heart diseases and in supporting the immune system; mucilage (approximate content of 80?g?kg?1), which is a mixture of neutral arabinoxylans and rhamnogalacturonans, with good water-holding capacities and high viscosity; phenolic compounds, such as p-coumaric and ferulic acids, lignan secoisolariciresinol, which is presented glycosylated (Figure 1) and/or esterified with 3-hydroxy-3-methylglutaric acid to form oligomers. The content of secoisolariciresinol diglucoside in flaxseed is 2-3?g?kg?1, and about 10–40?g?kg?1 in defatted flaxseed powder [1–5]. Figure 1: Lignan secoisolariciresinol diglucoside. In humans and animals, secoisolariciresinol is transformed by the anaerobic intestinal microflora into the mammalian lignans, enterolactone, and enterodiol, which are capable of binding at low levels to estrogen receptors. Additionally, these lignans have antioxidant, hypocholesterolemic, and antiatherosclerotic activities and inhibit the development of type 1 and type 2 diabetes, and mammary, prostatic, and colonic tumors [3, 6–9]. Lignans
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