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Search Results: 1 - 10 of 201607 matches for " Marcos Tulio Díaz "
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Paragonimus mexicanus en hospederos naturales en el Estado Sucre, Venezuela.
Marcos Tulio Díaz y,Maira Marin,Erika Gómez,Antulio Prieto
Salus Online , 2011,
Abstract: Paragonimus mexicanus in natural hosts from two municipalities of the Sucre State, Venezuela.The Paragonimus mexicanus infection was studied in the opposum Didelphis marsupialis and crab Eudaniela garmani from the Sucre and Montes municipalities of Sucre State, Venezuela. In Sucre,prevalence of adult parasites in opposums was 13. 33% with an intensity of 1.50 parasites per individual; in Montes, prevalence was 31.88% with a parasite load of 6.82 parasites per host. Prevalence of metacercariae in crabs was 17. 98% in Montes and 4.07% in Sucre. No significant differences between prevalence, intensity and gender of the crabs was found in any of the two areas, but there was a positive and significant correlation with size. In Sucre, the crab parasite burden was higher, 8.20%, than in Montes,5.31%. It was concluded that the intensity of infection was higher in female crabs and in those of a larger body size. These results indicate the existence of zoonotic contamination in both areas, and the need tocontinue investigating in order to determine relevant elements of this parasitic interrelation.
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela Immunodiagnostic in Didelphis marsupialis using an antigen of Venezuelan Paragonimus
Erika Gómez Martínez,Zoraida Díaz-Bello,Reinaldo Zavala-Jaspe,Marcos Tulio Díaz
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: Los Paragonimus son trematodos que habitualmente viven en los pulmones de mamíferos carnívoros y omnívoros, entre ellos el hombre. En el oriente venezolano se encuentra el único foco de Paragonimus sp. donde Didelphis marsupialis es el único reservorio demostrado hasta ahora. Con el fin de tener herramientas de inmunodiagnóstico que detecten la presencia de Paragonimus sp. en esta especie, se elaboraron varios reactivos para realizar un ensayo inmunoenzimático ELISA. Entre ellos se obtuvo un antígeno crudo soluble de vermes adultos de Paragonimus y una inmunoglobulina de gallina anti-IgG de Didelphis marsupialis. Los mismos se capturaron en la localidad de Aguas Blancas, municipio Montes, estado Sucre, Venezuela, y se obtuvieron muestras sanguíneas; en el caso de estar infectados, los vermes adultos se extrajeron del pulmón. Los parásitos se homogenizaron y ultracentrifugaron para obtener la fracción soluble del parásito (FSPA) como antígeno para el ELISA y Western blot y detectar los anticuerpos en los Didelphis marsupialis. El análisis electroforético mostró 22 moléculas entre 6 y 82 kDa; por Western blot se presentó un reconocimiento antigénico de 8 moléculas siendo las de 112 kDa y 268 kDa las más reconocidas por los sueros positivos. Los sueros negativos no reconocieron ninguna proteína. La producción de IgY en gallinas permitió desarrollar las técnicas de inmunodiagnóstico para la búsqueda de anticuerpos específicos anti-Paragonimus sp. en Didelphis, cuya aplicación permitirá establecer la vigilancia epidemiológica de estos reservorios en áreas endémicas sin sacrificio de los mismos. Paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. An outbreak of Paragonimus sp. in which Didelphis marsupialis was the only wild reservoir incriminated was described in eastern Venezuela. In order to have immunological tools to detect the presence of Paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. Didelphis marsupialis were captured in the locality of Aguas Blancas, Montes municipality, Sucre state, Venezuela, from which blood samples were obtained and a search for worms was performed in lungs. Worms were homogenized and ultracentrifugated to obtain FSPA to perform immunoassay (ELISA) to detect antibodies in opossums. The electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kDa; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kDa and 268 kDa molecules being the most strongly recognized by positive
Inmunodiagnóstico en Didelphis marsupialis usando un antígeno de Paragonimus de Venezuela
Gómez Martínez,Erika; Díaz-Bello,Zoraida; Zavala-Jaspe,Reinaldo; Tulio Díaz,Marcos; Noya,Oscar; Alarcón de Noya,Belkisyole;
Acta bioqu?-mica cl?-nica latinoamericana , 2010,
Abstract: paragonimus are trematodes that normally live in the lungs of carnivorous and omnivorous mammals such as humans. an outbreak of paragonimus sp. in which didelphis marsupialis was the only wild reservoir incriminated was described in eastern venezuela. in order to have immunological tools to detect the presence of paragonimus sp. in this reservoir, a whole antigen of the adult worm of this parasite was elaborated. didelphis marsupialis were captured in the locality of aguas blancas, montes municipality, sucre state, venezuela, from which blood samples were obtained and a search for worms was performed in lungs. worms were homogenized and ultracentrifugated to obtain fspa to perform immunoassay (elisa) to detect antibodies in opossums. the electrophoresis analysis showed a pattern of 22 molecules between 6 and 82 kda; by western blot, the antigenic recognition of 8 antigenic molecules appeared,112 kda and 268 kda molecules being the most strongly recognized by positive sera. the negative sera did not recognize any band. the production of igy in chicken enabled the development of reagents capable of performing a standard immunodiagnosis technique to find specific anti-paragonimus sp. in didelphis marsupialis in order to establish epidemiological surveillance of these reservoirs in endemic areas.
Diagnóstico de Dirofilaria immitis en el municipio Sucre, estado Sucre, Venezuela
Guilarte,Del Valle; Gómez Martínez,Erika; El Hen,Fabiana; Guzmán,Roselvia; Blondell,Deborah; Díaz,Marcos Tulio; Santiago,Javier;
Boletín de Malariología y Salud Ambiental , 2011,
Abstract: heartworm is a parasitic disease, caused by nematodes dirofilaria immitis and d. repens, mainly. while d. immitis presents worldwide distribution, d. repens extends the old world. both species affect canines and felines domestic and wild, as well as human, so it is a global public health problem. in this study we determined the prevalence of d. immitis in dogs in the municipality of sucre, sucre state, venezuela. blood samples were taken for 138 domestic dogs, over six months, regardless of race or sex, prior informed consent by their owners. the parasitological diagnosis of circulating microfilariae was done by direct examination and concentration method modified knott, the morphological characteristics were observed in giemsa-stained smears. we used a commercial kit, for the capture of excretory-secretory antigens of d. immitis. of the 138 dogs studied, 12 cases (8.7%) were detected parasitologically, whereas the commercial kit antigens were detected in 20 dogs (14.5%), demonstrating that there is an occult infections in 5.8% of the cases. one of the dogs with microfilariae in blood, did not submit antigens circulating. the prevalence of heartworm in the municipality of sucre, sucre state was 15.2%. this report of canine heartworm in the municipality of sucre, represents an important contribution in an epidemiologic survey of the disease. future studies will assess the risk of infection for people living with dogs with heartworm disease.
Diversidad enzimática de la fracción soluble de un aislado venezolano de adultos Paragonimus sp
Gómez Martínez,Erika; Ballén,Diana; Tulio Díaz,Marcos; Díaz Bello,Zoraida; Zavala-Jaspe,Reinaldo; Alarcón de Noya,Belkisyolé; Noya,Oscar; Cesari,Italo M;
Boletín de Malariología y Salud Ambiental , 2010,
Abstract: paragonimus sp. is a trematode that causes chronic inflammation of the lung in carnivorous mammals and humans, which constitute a public health problem in asian and latin american countries. trematodes have enzymes that facilitate their penetration and migration through different host organs to ensure their life cycle. to evaluate the enzymatic diversity of the soluble fraction (fspa, 100,000 g) of a venezuelan isolate of paragonimus sp. adult worms, several enzyme determinations were conducted at different ph. the activities of enzymes releasing p-nitrophenol or p-nitroanilina from the corresponding dye-related synthetic peptides were assessed by interpolating absorbance (a 405 nm) values in the corresponding calibration curve (a 405 nm vs. nmol); on the other hand, absorbances of 2-naphtylamine and 2-naphtols released from another series of synthetic substrates were read at different wavelengths between 450 nm and 620 nm to assess for the activity of the corresponding hydrolases. phosphohydrolase, glycosidase and peptidase activities were detected in fspa, β-n-acetyl-β-d-glucosaminidase (0.55 μmol/h/mg, ph 5.5) and cystein protease (0.4 μmol/h/mg, ph 5.5) being higher than all the other detected activities. these activities are probably related to the adult worm habitat and its need for glycan and peptide degradation of lung secretions. these results represent the first enzymatic study done with a venezuelan isolate of adult paragonimus sp. worms collected from the common reservoir didelphis marsupialis.
El salto generacional y el discurso social en El Graduado
Díaz Martín, Marcos
Fonseca : Journal of Communication , 2012,
Abstract: En espa ol: El Graduado es una de las películas más representativas del movimiento cinematográfico denominado New Hollywood dado en Estados Unidos a finales de la década de los sesenta y principios de los setenta por varios motivos: la gran audiencia y repercusión mediática que cosechó durante su exposición cinematográfica, la diversidad que originó en el terreno de la crítica especializada y el estatus icónico que desde entonces mantiene. Basándonos en la teoría del cine de temática contemporánea como representante más o menos fiel de los acontecimientos históricos y sociales que le rodean, en este artículo analizamos la suficiencia del filme de Mike Nichols como evidencia del cambio cultural y el salto generacional dado en la época y analizamos su carácter de película revolucionaria -haciendo hincapié tanto en su relación con la contracultura como en su controvertido final- desde el punto de vista ideológico y de la caracterización de los personajes. In english: The Graduate is one of the most representative films of late sixties and early seventies cinema movement New Hollywood in the United States for several reasons: the large audience and media impact during its showing, the disagreements in the specialized criticism and the iconic status it has since then. Based on the theory of films as representations of their contemporary historical and social events, in this paper we analyze the value of Mike Nichols film as evidence of the cultural change and generation gap of the period. We analyze its revolutionary character -paying attention both to its relation with counterculture and to its controversial ending- from the ideological and character description points of view.
Synthesis and characterization of new 3-substituted thiophene copolymers
Alves, Marcos R. A;Calado, Hállen D. R;Donnici, Claudio L;Matencio, Tulio;
Journal of the Brazilian Chemical Society , 2011, DOI: 10.1590/S0103-50532011000200009
Abstract: this work reports the synthesis of three polymers and two new copolymers based on 3-substituted polythiophene derivatives, all of them demonstrated photoluminescent properties. all polymers synthesized, i.e., poly(3-methoxythiophene) (pmot), poly(3-thiopheneethanol) (pte), poly(3-thiophenemethanol) (ptm), poly(3-methoxythiophene-co-3-thiopheneethanol) (pmot-co-te) and poly(3-methoxythiophene-co-3-thiophenemethanol) (pmot-co-tm), were characterized by ft ir, uv-vis, photoluminescence (pl), thermal analysis (tg and dsc) and x-ray diffraction (xrd). all materials emitted light in the orange region of the visible spectrum. spectrometric analysis in the infrared region demonstrated the successful synthesis of polymers and copolymers by the presence of characteristic bands. thermal analysis showed that the obtained materials had good thermal stability.
Caracterización de adultos mayores hospitalizados con lesiones traumáticas osteomioarticulares
Moreno Navarro,Jorge Tulio; Fong Estrada,Adela; Martínez López,Ricardo; Ciudad Díaz,Olga L;
MEDISAN , 2011,
Abstract: a descriptive and cross-sectional study of 109 elderly hospitalized at orthopedics and traumatology service from "dr ambrosio grillo portuondo" teaching clinical-surgical hospital in santiago de cuba due to osteomioarticular traumatic injuries was carried out during the first semester of 2009. interest variables for the research included: age, sex, medical history, geriatric scale of functional assessment, injury type, treatment, and complications. it was found that the patients most affected by such trauma were women and those aged persons over 70 years old. hip fracture was the most common injury. in spite of having associated diseases, they were active people and barely experienced complications.
Caracterización de adultos mayores hospitalizados con lesiones traumáticas osteomioarticulares Characterization of aged adults hospitalized due to osteomioarticular traumatic injuries
Jorge Tulio Moreno Navarro,Adela Fong Estrada,Ricardo Martínez López,Olga L Ciudad Díaz
MEDISAN , 2011,
Abstract: Se realizó un estudio descriptivo y transversal de los 109 ancianos ingresados a causa de lesiones traumáticas osteomioarticulares en el Servicio de Ortopedia y Traumatología del Hospital Clinicoquirúrgico Docente "Dr. Ambrosio Grillo Portuondo" de Santiago de Cuba, durante el primer semestre de 2009. Las variables de interés para la investigación incluyeron: edad, sexo, antecedentes patológicos, escala geriátrica de evaluación funcional, tipo de lesión, tratamiento y complicaciones. Se encontró que los pacientes más afectados por tales traumatismos resultaron ser las mujeres y los mayores de 70 a os, que la fractura de cadera fue la lesión más común y que a pesar de que solían presentar enfermedades asociadas, eran activos y experimentaban escasas complicaciones. A descriptive and cross-sectional study of 109 elderly hospitalized at Orthopedics and Traumatology Service from "Dr Ambrosio Grillo Portuondo" Teaching Clinical-Surgical Hospital in Santiago de Cuba due to osteomioarticular traumatic injuries was carried out during the first semester of 2009. Interest variables for the research included: age, sex, medical history, geriatric scale of functional assessment, injury type, treatment, and complications. It was found that the patients most affected by such trauma were women and those aged persons over 70 years old. Hip fracture was the most common injury. In spite of having associated diseases, they were active people and barely experienced complications.
Caracterización de la mortalidad en la unidad de cuidados intensivos: A?o 1998
álvarez Figueredo,Zoraida; Iraola Ferrer,Marcos D; Molina Díaz,Félix; Barco Díaz,Vladimir;
Revista Cubana de Medicina , 2000,
Abstract: a retrospective descriptive study of patients who died in 1998, with the objective of characterizing mortality in the intensive care units of ?dr gustavo aldereguía lima? university hospital in cienfuegos was performed. the required data were taken from the monthly report by the in-hospital mortality analysis committee. it was observed that the average age of dead patients was 52,50 ± 21,28 years, most of the patients came from both emergency medical services (49%) and the surgery room (25.5%) and that medical admissions exceeded surgical ones. average stay length was 6.53± 9.82 days. the main cause of death was found to be acute myocardial infarction (27.6%) followed by trauma ( 18.32%). there was a complete clinical and pathological concordance in 71.6% of the dead people. it was proved that lethality of patients who died from trombolized acute myocardial infarction was 8% and that of the non-trombolized one was 26%; whereas lethality of head trauma with or without multiple injuries was 62.5% and that of multiple injuries without head trauma was 8.5%
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