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Search Results: 1 - 10 of 276 matches for " Marciane Magnani "
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Beta-glucana from Saccharomyces cerevisiae: constitution, bioactivity and obtaining / Beta-glucana de Saccharomyces cerevisiae: constitui o, bioatividade e obten o
Marciane Magnani,Raul Jorge Hernan Castro-Gómez
Semina : Ciências Agrárias , 2008,
Abstract: b-glucans are polysaccharides that constitute the structure of the cell wall of yeast, fungi and some cereals, which differs each other by the linkages between glucose units. An important source of these polymers is the Saccharomyces cerevisiae cell wall, which is a yeast widely used in industrial processes of fermentation. The b-glucan is considered to be a modifier of biological response due to its immunomodulator potential. When it is recognized by specific cellular receptors, have the ability to enhance the host’s immune response. Other beneficial effects such as anticarcinogenic, antimutagenic, hypocholesterolemic and blood sugar reduction have also been related to the b-glucan. The aim of this literature review was expand scientific knowledge about the constitution and bioactivity of b-glucan, including its recognition by the immune system, as well as its obtaining from S. cerevisiae cell wall. b-glucanas s o polissacarídeos constituintes estruturais da parede celular de leveduras, fungos e alguns cereais, que se diferenciam pelo tipo de liga o presente entre as unidades de glicose. Uma importante fonte destes polissacarídeos é a parede celular de Saccharomyces cerevisiae, uma levedura amplamente empregada em processos industriais de fermenta o. A b-glucana é considerada um modificador da resposta biológica devido ao seu potencial imunomodulador, pois ao ser reconhecida por receptores celulares específicos tem habilidade de real ar a resposta imune do hospedeiro. Outros efeitos benéficos como anticarcinogênico, antimutagênico, hipocolesterolêmico e hipoglicêmico também têm sido relacionados à b-glucana Esta revis o de literatura teve por objetivo agregar conhecimentos científicos sobre a constitui o e bioatividade da b glucana, incluindo seu reconhecimento pelo sistema imune, bem como, a obten o a partir da parede celular de S. cerevisiae.
Evaluation of potential immunostimulant of the Carboxymethyl-glucan from Saccharomyces cerevisiae in poultry (Gallus domesticus) / Avalia o do potencial imunoestimulante da Carboximetil-glucana de Saccharomyces cerevisiae em frangos de corte (Gallus domesticus)
Fernanda Patrícia Brito Darpossolo,Leonardo Raffa Quintana,Marciane Magnani,Alexandre Oba
Semina : Ciências Agrárias , 2010,
Abstract: The carboxymethylglucan (CMG) is a soluble molecule, composed of glucopyranosyl linked by ?(1-3) e ?(1-6), which can activate the immune system of the host. The purpose of this study was evaluate the productive and immunological characteristics of 192 poultry (Gallus domesticus) COBB line which received feds containing 0%, 0,025%, 0,050% e 0,075% of CMG from Saccharomyces cerevisiae added in corn flour. All poultry were immunized against Newcastle disease and at each treatment 3 poultries randomly chosen received CMG intramuscular at 3, 7 and 14 days. It was evaluated the animal performance, development of the bursa of Fabricius, histological slides of the small intestine, counts of phagocytes cells in blood and levels of antibodies in serum. The results showed difference in weight gain and consumption of feed to poultry that consumed CMG at 1 to 21 days. Fabricius bursa relative weight increased in poultry supplemented with 0,025 e 0,050% of CMG. The phagocytic cells number and total levels of antibodies found in poultry at 21 days were higher in those that received CMG in the diet. For the animals that received intramuscular CMG was observed increase of antibodies specific to Newcastle. A carboximetilglucana (CMG) é uma molécula solúvel, composta de resíduos de glicopiranosil unidos em ?(1-3) e ?(1-6), que possui a capacidade de ativar o sistema imune do hospedeiro. O objetivo do presente estudo foi avaliar as características produtivas e imunológicas de 192 frangos de corte (Gallus domesticus) da linhagem COBB, que receberam ra es contendo 0%, 0,025%, 0,050% e 0,075% de CMG de Saccharomyces cerevisiae adicionada em farinha de milho. Todas as aves foram imunizadas contra a doen a de Newcastle e, em cada tratamento, 3 aves escolhidas aleatoriamente receberam CMG intramuscular no 3o, 7o e 14o dia. Foram avaliados o desempenho animal, o desenvolvimento da bursa de Fabricius e laminas histológicas do intestino delgado, além do número de células fagocíticas no sangue e níveis de anticorpos no soro. Os resultados mostraram diferen a no ganho de peso e consumo de ra o das aves que consumiram a CMG no período de 1 a 21 dias. O peso relativo da bursa de Fabricius aumentou nas aves suplementadas com 0,025 e 0,050% de CMG. O número de células fagocíticas e os níveis de anticorpos totais dos frangos de corte, aos 21 dias de idade foram maiores nas aves que receberam CMG na ra o. Para os animais que receberam CMG via intramuscular foi observado aumento no título dos anticorpos específicos para Newcastle.
In vitro and in vivo toxicity studies of Agrobacterium radiobacter k84 biopolymer (ARB) Estudos in vitro e in vivo de toxicidade de biopolímero de Agrobacterium radiobacter k84 (ARB)
Caroline Maria Calliari,Marciane Magnani,Alexandre Ykuio Saito,Pedro Sebasti?o Dionízio Filho
Semina : Ciências Agrárias , 2011,
Abstract: Sugar cane molasses is a cheaper carbon source alternative than glucose traditionally used in fermentation processes. In the present study a biopolymer soluble from Agrobacterium radiobacter k84 (ARB) was obtained by fermentation using sugar cane molasses as a carbon source in a process with yield of 10.0 g.L-1. The ARB is composed by minerals (40%), carbohydrate (35%) and protein (15%). In vitro test of the cytotoxic effect of ARB at concentrations 2.5 mg/mL, 5.0 mg/mL and 10.0 mg/mL in LLC MK2 (Rhesus Monkey Kidney) cells revealed a 50% cytotoxic concentration (CC50) of 9.32 mg/mL. In a 30-day in vivo oral toxicity study, Swiss mice were administered ARB by gavage at 5 mg/mL, 15 mg/mL, 50 mg/mL and 150 mg/mL (approximately 25 mg/kg/day, 75 mg/kg/day, 250 mg/kg/day and 750 mg/kg/day). The results did not present any hematological or histopathological signs of adverse effects, leading us to define the no observed adverse effect level (NOAEL) as 150 mg/mL (approximately 750 mg/kg/day). O mela o de cana-de-a úcar é uma fonte de carbono alternativa de menor custo que a glicose tradicionalmente utilizada em processos fermentativos. No presente estudo, um biopolímero solúvel de Agrobacterium radiobacter k84 (ARB) foi obtido por fermenta o utilizando mela o de cana de a úcar como fonte de carbono em um processo com rendimento de 10,0 g.L-1. O ARB é composto de minerais (40%), carboidratos (35%) e proteínas (15%). O teste do efeito citotóxico do ARB in vitro nas concentra es de 2,5 mg/mL, 5,0 mg/mL e 10,0 mg/mL em células LLC MK2 (Rim de Macaco Rhesus) revelou uma concentra o citotóxica 50% (CC50) de 9,32 mg/mL. Em estudo in vivo de toxicidade oral durante 30 dias, camundongos Swiss receberam por gavagem solu es de ARB nas concentra es de 5 mg/mL, 15 mg/mL, 50 mg/mL e 150 mg/mL (aproximadamente 25 mg/kg/dia, 75 mg/kg/dia, 250 mg/ kg/dia e 750 mg/kg/dia). Os resultados n o apresentaram sinais hematológicos ou histopatológicos de efeitos adversos, levando a definir a dose sem efeito adverso observado (NOAEL) como 150 mg/mL (aproximadamente 750 mg/kg/dia).
Mechanisms of quinolone resistance in Salmonella spp. / Mecanismos de resistência às quinolonas em Salmonella spp.
Roberta Barreiros de Souza,Marciane Magnani,Tereza Cristina Rocha Moreira de Oliveira
Semina : Ciências Agrárias , 2010,
Abstract: Salmonellosis is a common and widespread zoonotic disease of humans and a frequent cause of foodborne disease. Treatment of severe and systemic salmonellosis is usually done with fluoroquinolones. In this review resistance mechanisms of Salmonella to quinolones are discussed. Single point mutations in the quinolone resistant determining region (QRDR) of the gyrA gene may be sufficient to generate high levels of resistance to non-fluorated quinolones and also may decrease the fluoroquinolones susceptibility. Other resistance mechanisms that should be considered are mutations in parC gene, the possibility of acquiring resistance through plasmidial transference and hyper-expression of efflux pumps. Fluoroquinolones resistance is still relatively uncommon in Salmonella compared to other species belonging to the Enterobacteriaceae family. However, the more careful use of fluoroquinolones in veterinary and human medicine is essential to decrease the selective pressure which can avoid the emergence and spread of resistant clones and consequently maintain the clinical efficacy of this group of antibiotics. A salmonelose é uma zoonose de importancia mundial e uma das mais freqüentes doen as de origem alimentar. As fluoroquinolonas s o a principal op o para o tratamento de salmoneloses graves ou sistêmicas. Esta revis o de literatura teve como objetivo apresentar os principais mecanismos envolvidos na resistência de Salmonella spp a estes antimicrobianos. Muta es de ponto na Regi o Determinante de Resistência à Quinolona (QRDR) do gene gyrA podem gerar altos níveis de resistência a quinolonas n o-fluoradas, além de reduzir a suscetibilidade as fluoroquinolonas. Outros mecanismos de resistência que também precisam ser considerados s o as muta es no gene parC, a possibilidade do envolvimento de plasmídios de resistência e o sistema de efluxo ativo. A resistência às fluoroquinolonas ainda é incomum em Salmonella spp., quando comparada a outros gêneros da família Enterobacteriaceae. No entanto, o uso criterioso de fluoroquinolonas na medicina humana e veterinária é essencial para reduzir a press o seletiva e evitar a emergência e dissemina o de clones resistentes, mantendo o espectro de a o e a eficácia clínica desta classe terapêutica.
Production, characterization and technological properties of biopolymer produced by Agrobacterium radiobacter k84 Produ o, caracteriza o e propriedades tecnológicas de um biopolímero produzido por Agrobacterium radiobacter k84
Caroline Maria Calliari,Marciane Magnani,Raúl Jorge Hernan Castro Gómez
Semina : Ciências Agrárias , 2011,
Abstract: In this study, a biopolymer composed of carbohydrates (35%), protein (15%) and minerals (40%) was obtained through fermentation using sugar cane molasses as the sole carbon source for Agrobacterium radiobacter k84. The process yield was 10 gL-1 of biopolymer, which showed high solubility in water, neutral pH in aqueous solution and low water activity (0.52). The analysis in Scanning Electron Microscopy revealed microstructure characteristic of an amorphous solid, with particles of irregular shapes and sizes. In the evaluation of technological properties, the biopolymer showed formation of viscous solutions at room temperature from concentration of 0.5% in aqueous solution, gelling activity in solution at 2%, emulsifying (56.11±1.39%) and stabilizing activity (98.02±0.78%). The results suggest that the biopolymer from Agrobacterium radiobacter k84 is a promising candidate for industrial use. No presente estudo, utilizando mela o de cana-de-a úcar como única fonte de carbono para Agrobacterium radiobacter k84 foi obtido, em processo fermentativo, um biopolímero composto por carboidratos (35%), proteínas (15%) e minerais (40%). O rendimento do processo foi de 10 g.L-1 do biopolímero que apresentou elevada solubilidade em água, pH neutro em solu o aquosa e baixa atividade de água (0.52). As análises em Microscopia Eletr nica de Varredura revelaram microestrutura característica de um sólido amorfo, com partículas de formas irregulares e tamanhos variáveis. Na avalia o das propriedades tecnológicas, o biopolímero mostrou viscosidade à temperatura ambiente a partir da concentra o 0.5% em solu o aquosa, atividade geleificante em solu o a 2%, atividade emulsificante (56.11±0.78%) e estabilizante (98.02±1.39%). Os resultados sugerem o biopolímero de Agrobacterium radiobacter k84 como um candidato promissor para uso industrial.
Microbiological and Nutritional Quality of the Goat Meat by-Product “Sarapatel”
Luciana Brasil,Angela Queiroz,Josevan Silva,Taliana Bezerra,Narciza Arcanjo,Marciane Magnani,Evandro Souza,Marta Madruga
Molecules , 2014, DOI: 10.3390/molecules19011047
Abstract: Goat “sarapatel” is a product made from blood and viscera. For the first time, the microbiological and nutritional quality of “sarapatel” samples ( n = 48) sold under different conditions (in street markets, butcher shops, and supermarkets under refrigeration, frozen or at room temperature) was evaluated. Goat “sarapatel” is a nutritive food, with each 100 g providing, on average, 72 g of moisture, 2 g of ash, 18 g of protein, 9 g of lipids, 2 g of carbohydrates, 282 mg of cholesterol, and high amounts of unsaturated fatty acids and essential amino acids. The analysis of the “sarapatel” samples shows that none of them contain Salmonella spp. or L. monocytogenes. High counts (>104) of total coliforms, thermotolerant coliforms, and sulfite-reducing Clostridium were detected, and coagulase-positive Staphylococcus was found in 31.25% of samples. The storage conditions evaluated (refrigeration, frozen or at room temperature) did not affect the physicochemical quality of the “sarapatel”; however, the unsatisfactory microbiological quality indicates that it is necessary to improve the health-sanitary aspects of the processing and sale of this product.
Protective effect of carboxymethyl-glucan (CM-G) against DNA damage in patients with advanced prostate cancer
Magnani, Marciane;Castro-Gomez, Raul Jorge Hernan;Mori, Mateus Prates;Kuasne, Hellen;Gregório, Emerson Pereira;Libos Jr, Farid;Cólus, Ilce Mara de Syllos;
Genetics and Molecular Biology , 2011, DOI: 10.1590/S1415-47572010005000103
Abstract: carboxymethyl-glucan (cm-g) is a soluble derivative from saccharomyces cerevisiae (1 → 3)(1 → 6)-β-d-glucan. the protective efficiency of cm-g against dna damage in cells from patients with advanced prostate cancer (pca), and undergoing androgen deprivation therapy (adt), was evaluated. dna damage scores were obtained by the comet assay, both before and after treatment with cm-g. the reduction in dna damage, ranging from 18% to 87%, with an average of 59%, was not related to the increased number of leukocytes in peripheral blood. the results demonstrate for the first time the protective effect of cm-g against dna damage in patients with advanced pca. among smokers, three presented the highest reduction in dna damage after treatment with cm-g. there was no observable relationship between dna damage scores before and after treatment, and age, alcoholism and radiotherapy.
Detection of quinolone-resistance mutations in Salmonella spp. strains of epidemic and poultry origin
Souza, Roberta Barreiros de;Magnani, Marciane;Ferrari, Rafaela Gomes;Kottwitz, Luciana Bill Mikito;Sartori, Daniele;Tognim, Maria Cristina Bronharo;Oliveira, Tereza Cristina R. M. de;
Brazilian Journal of Microbiology , 2011, DOI: 10.1590/S1517-83822011000100026
Abstract: mutations into codons aspartate-87 (62%) and serine-83 (38%) in qrdr of gyra were identified in 105 salmonella strains resistant to nalidixic acid (94 epidemic and 11 of poultry origin). the results show a high incidence of mutations associated to quinolone resistance but suggest association with others mechanisms of resistance.
Ciprofloxacin susceptibility reduction of Salmonella strains isolated from outbreaks
Souza, Roberta B.;Ferrari, Rafaela G.;Magnani, Marciane;Kottwitz, Luciana B. M.;Alcocer, Iliana;Tognim, Maria Cristina B.;Oliveira, Tereza C. R. M.;
Brazilian Journal of Microbiology , 2010, DOI: 10.1590/S1517-83822010000200033
Abstract: the antimicrobial susceptibility of 212 salmonella strains isolated from patients and foods was evaluated and 45% were found to be resistant to nalidixic acid. nalidixic acid resistant strains showed a higher minimal inhibitory concentration for ciprofloxacin than sensitive strains. during the study an increase of strains with reduced susceptibility to ciprofloxacin was also observed.
Molecular identification of Aspergillus spp. isolated from coffee beans
Magnani, Marciane;Fernandes, Thiago;Prete, Cássio Egidio Cavenaghi;Homechim, Martin;Ono, Elisabete Yurie Sataque;Vilas-Boas, Laurival Antonio;Sartori, Daniele;Furlaneto, Márcia Cristina;Fungaro, Maria Helena Pelegrinelli;
Scientia Agricola , 2005, DOI: 10.1590/S0103-90162005000100009
Abstract: some species belonging to the genus aspergillus are potential producers of ochratoxin a (oa), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. the aim of the present study was to identify the species of aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the state of paraná, in the south of brazil. a total of 108 isolates of aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (its1-5.8s-its2) of ribosomal dna (rdna). the results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which aspergillus niger was the species most frequently detected, followed by a. ochraceus and a. carbonarius. the presence of a. carbonarius in immature coffee fruits harvested from trees is reported for the first time.
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