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Search Results: 1 - 10 of 558144 matches for " María; Greco B "
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IDENTIFICACIóN DE ESPECIES CáRNICAS EN PRODUCTOS CáRNICOS COCIDOS: UTILIZACIóN DE SDS-PAGE COMO MéTODO DE SCREENING MEAT SPECIES IDENTIFICATION IN COOKED MEAT PRODUCTS: USING SDS-PAGE AS SCREENING METHOD
Laura López B,María Binaghi J,Carola Greco B,María Mambrín C
Revista Chilena de Nutricíon , 2011,
Abstract: Es necesario contar con metodologías que permitan la detección de las especies cárnicas utilizadas en la elaboración de productos cárnicos a los fines de establecer posibles adulteraciones. Con la finalidad de evaluar SDS-PAGE como método de screening para identificar la/las especies cárnicas utilizadas" en el presente trabajo" se analizaron veinte productos cárnicos crudos o cocidos elaborados en plantas piloto o comerciales. Los resultados hallados por electroforesis se compararon con un método inmunoquímico (ELISA). SDS-PAGE permitió la detección de proteínas de carne vacuna" porcina" de pollo y/o de pavo en las mayoría de las muestras analizadas. Sólo en algunas muestras que contenían alguna de las especies cárnicas en baja proporción" esta metodología no permitió su detección. SDS-PAGE tiene como ventaja poder detectar en una sola corrida la presencia de proteínas de diferentes especies cárnicas" mientras que con ELISA es necesario analizar una misma muestra con los diferentes kits de especies cárnicas para confirmar la presencia de las especies utilizadas. It is necessary to have methodologies for the detection of meat species used in meat products in order to establish possible adulterations. Twenty cooked meat products produced in pilot plants or commercially available products were analyzed in order to evaluate the SDS-PAGE electrophoresis methodology as a screening method to identify the meat species used. The results found by electrophoresis were compared with an immunochemical method (ELISA kits for detection ofpork, beef and poultry). SDS-PAGE methodology allowed the detection of beef, pork, chicken and /or turkey proteins in most samples. When some of these species were present in low proportion this methodology was not able to detect them. SDS-PAGE method has the advantage that allows the detection of proteins of different meat species in only one electrophoresis run while with the ELISA method it is necessary to analyze the same sample with different species identification kits to confirm the presence of the species used.
IDENTIFICACIóN DE ESPECIES CáRNICAS EN PRODUCTOS CáRNICOS COCIDOS: UTILIZACIóN DE SDS-PAGE COMO MéTODO DE SCREENING
López B,Laura; Binaghi J,María; Greco B,Carola; Mambrín C,María; Valencia E,Mirta;
Revista chilena de nutrición , 2011, DOI: 10.4067/S0717-75182011000200009
Abstract: it is necessary to have methodologies for the detection of meat species used in meat products in order to establish possible adulterations. twenty cooked meat products produced in pilot plants or commercially available products were analyzed in order to evaluate the sds-page electrophoresis methodology as a screening method to identify the meat species used. the results found by electrophoresis were compared with an immunochemical method (elisa kits for detection ofpork, beef and poultry). sds-page methodology allowed the detection of beef, pork, chicken and /or turkey proteins in most samples. when some of these species were present in low proportion this methodology was not able to detect them. sds-page method has the advantage that allows the detection of proteins of different meat species in only one electrophoresis run while with the elisa method it is necessary to analyze the same sample with different species identification kits to confirm the presence of the species used.
Restructuración productiva y alteraciones en los patrones migratorios tradicionales
Greco,María Gabriela;
Cuadernos de la Facultad de Humanidades y Ciencias Sociales. Universidad Nacional de Jujuy , 2000,
Abstract: the changes occurred across the last decades in spaces and population of several countries, show major transformations related to productive forces and organization. probably one of the most dramatic changes is represented by manpower demand reduction from certain activities like salta and jujuy's sugar industry, among others. so, the main questions that appear are the following: what happen with the population that is displaced from its activity and productive function? what happen specifically with exceeding labor force when the socioeconomic system denied its possibilities of economic reproduction? the current analysis was done departing from the population from santa victoria oeste, a department site in the north of salta province. this area has functioned providing transitory labor force for salta and jujuy's sugar industry, since the early decades from this century. sugar industry, since the 70's have reduced considerably the incorporation of temporary workers, pushing them to look for new labor alternatives. this search, in several cases, involve new migratory process to spaces not only rural but also urban, and different situations that goes from temporary proletarization, the occasional wage-earnering or the ''cuentapropismo'', in several opportunities in spaces located long far away form their original habitat.
Diversity of biting midges of the genus Culicoides Latreille (Diptera: Ceratopogonidae) in the area of the Yacyretá Dam Lake between Argentina and Paraguay
Ronderos, María M;Greco, Nancy M;Spinelli, Gustavo R;
Memórias do Instituto Oswaldo Cruz , 2003, DOI: 10.1590/S0074-02762003000100003
Abstract: the culicoides communities have been analyzed between 1993/1998 in the area influenced by the yacyretá dam lake (paraná river, argentina-paraguay). adults of culicoides were collected monthly by using cdc light traps exposed for 24 h in 9 sampling sites located at both margins of the river; 21 species were recorded. highest values of species richness were recorded during 1993/1994, being quiteria and corpus the sites with the higest number of species (10 and 11, respectively). the species diversity was elevated in quiteria, zaimán, candelaria, santa tecla, capitán meza and corpus (shannon's diversity index 1.0-1.9) while corateí, ituzaingó and aguapey showed less richness and diversity. the more abundant species were c. insignis, c. venezuelensis, c. leopoldoi, c. limai, c. flinti, c. debilipalpis, c. paraensis and c. guttatus. c. insignis, potential vector of bluetongue virus (btv) to domestic and wild rumiants in the neotropical region, is the predominant species in the area and was the only species widely distributed. c. paraensis, a proven vector of oropouche virus to humans, is a common and abundant species. c. pusillus and c. lahillei, potential vectors of btv and a filarial parasite, respectively, were occasionally collected. the taxonomic structure of communities was constant during the study period. the occasional species were not characteristic to one particular site and their presence could be related to non-intrinsic conditions.
Diversity of biting midges of the genus Culicoides Latreille (Diptera: Ceratopogonidae) in the area of the Yacyretá Dam Lake between Argentina and Paraguay
Ronderos María M,Greco Nancy M,Spinelli Gustavo R
Memórias do Instituto Oswaldo Cruz , 2003,
Abstract: The Culicoides communities have been analyzed between 1993/1998 in the area influenced by the Yacyretá Dam Lake (Paraná River, Argentina-Paraguay). Adults of Culicoides were collected monthly by using CDC light traps exposed for 24 h in 9 sampling sites located at both margins of the river; 21 species were recorded. Highest values of species richness were recorded during 1993/1994, being Quiteria and Corpus the sites with the higest number of species (10 and 11, respectively). The species diversity was elevated in Quiteria, Zaimán, Candelaria, Santa Tecla, Capitán Meza and Corpus (Shannon's diversity index 1.0-1.9) while Corateí, Ituzaingó and Aguapey showed less richness and diversity. The more abundant species were C. insignis, C. venezuelensis, C. leopoldoi, C. limai, C. flinti, C. debilipalpis, C. paraensis and C. guttatus. C. insignis, potential vector of bluetongue virus (BTV) to domestic and wild rumiants in the Neotropical region, is the predominant species in the area and was the only species widely distributed. C. paraensis, a proven vector of Oropouche virus to humans, is a common and abundant species. C. pusillus and C. lahillei, potential vectors of BTV and a filarial parasite, respectively, were occasionally collected. The taxonomic structure of communities was constant during the study period. The occasional species were not characteristic to one particular site and their presence could be related to non-intrinsic conditions.
Diferen?as nas situa??es de risco para HIV de homens bissexuais em suas rela??es com homens e mulheres
Greco,Marília; Silva,Ana P.; Merchán-Hamann,Edgar; Jeronymo,Mauro L.; Andrade,Julio C.; Greco,Dirceu B.;
Revista de Saúde Pública , 2007, DOI: 10.1590/S0034-89102007000900017
Abstract: objective: to describe bisexual men's behavior in terms of sexual identity, condom use, frequency of sexual intercourse and types of partners and to determine rates of inconsistent condom according to partner's gender. methods: cross-sectional study nested in a cohort of hiv-negative homosexual and bisexual men in the city of belo horizonte, southeastern brazil, followed up since 1994 (horizonte project). of 1,025 subjects enrolled between 1994 and 2005, 195 volunteers who reported at admission having sexual relations with men and women during the previous six months were selected. a behavioral risk index, called horizonte risk index, was estimated. it incorporates a constant assigned to each type of unprotected sexual act, adjusted for the number of sexual encounters. results: sexual activity with men predominated; most considered themselves as bisexual (55%) and homosexual (26%). during the six months prior to the study, median number of casual male partners (4) was higher than both casual female partners (2) and steady male or female partners (1). during vaginal sex with a steady partner, the rate of inconsistent condom use was 55%, compared to 35% and 55% in anal insertive and anal receptive sex, respectively, with steady male partners. the index was higher for those having sex with men and women compared to those having sex either exclusively with women or men (p=0.004). conclusions: hiv risk behavior was more frequent among men who reported sexual activity both with men and women. bisexual men display different sexual and protective behavior according to gender and steadiness of relationships, and female steady partners had more unprotected encounters.
Salazones y chacinados embutidos secos: detección por electroforesis de especies cárnicas y de proteínas extrínsecas agregadas Dry-cured meat products: Electrophoresis detection of meat species and extrinsic proteins added
Laura Beatriz López,María Julieta Binaghi,Carola Beatriz Greco,María Cecilia Mambrín
Diaeta , 2010,
Abstract: En la elaboración de salazones y chacinados embutidos secos se pueden utilizar diferentes especies cárnicas y en algunos de ellos también se pueden agregar proteínas extrínsecas. En el presente trabajo se plantearon los siguientes objetivos: analizar salazones y chacinados embutidos secos elaborados con especies cárnicas de diferente origen (vacuna, porcina, de ciervo, de jabalí y de cordero) para establecer la utilidad de SDS-PAGE en la identificación de las mismas; comparar esta metodología con un método inmunoquímico (ELISA para especie porcina y ELISA para especie vacuna) en muestras que declaraban carne porcina y/o vacuna; detectar la posible presencia de proteínas extrínsecas declaradas o no en los respectivos rótulos de estos productos. Se analizaron 5 salazones y 7 chacinados embutidos secos. En todas las muestras se detectaron por electroforesis la o las especies cárnicas declaradas, sólo en una muestra que declaraba carnes vacuna y porcina se detectó sólo carne vacuna. La metodología inmunoquímica confirmó la detección de las carnes vacuna y/o porcina en las muestras que las declaraban. Con respecto a las proteínas extrínsecas se detectaron por electroforesis proteínas de soja en tres muestras, dos de ellas no las declaraban. Se detectaron proteínas lácteas en muy baja concentración en tres muestras que las declaraban y se detectaron proteínas de trigo en dos muestras que no las declaraban. Las tres proteínas detectadas en estas muestras constituyen alergenos alimentarios. Si bien el método ELISA resulta de elección para la detección de alergenos alimentarios, ya que tiene una sensibilidad mucho mayor al SDS-PAGE, estas proteínas alergénicas fueron detectadas con facilidad por electroforesis, lo cual indica que estaban agregadas en concentraciones importantes. Resulta entonces imprescindible que los elaboradores de este tipo de productos declaren la totalidad de los ingredientes proteicos utilizados para evitar reacciones alérgicas en los consumidores. Different meat species can be used in dry-cured meat products, and extrinsic proteins can also be added in some of them. The objectives of this work are to analyze dry-cured meat products elaborated with different meat species (cow, swine, deer, boar and lamb) in order to evaluate the usefulness of the SDS-PAGE method in the identification of the species used; to compare this methodology with an immunochemical method (ELISA for porcine and bovine species respectively) in samples that declare porcine and/or bovine meat in their label; and to detect the possible presence of extrinsic proteins decla
Estimación de proteína potencialmente utilizable en fórmulas infantiles de inicio para neonatos prematuros y de término
Binaghi,María J; Baroni,Andrea; Greco,Carola; Ronayne de Ferrer,Patricia A; Valencia,Mirta;
Archivos Latinoamericanos de Nutrición , 2002,
Abstract: estimation of potentially available protein in starting formulas for term and preterm infants. sixteen milk-based starting formulas were analyzed with the aim of calculating their "true protein" content and assessing "in vitro" protein digestibility, in order to estimate levels of potentially available protein. ten of them were designed for term infants: 7 had a casein:whey protein ratio 40:60 (adapted formulas) and 3 a ratio 80:20 (non-adapted); the 6 remaining formulas (all adapted) were for preterm infants. nitrogen was determined by the kjeldahl method. true protein was calculated as (total n- non-protein n) x 6.25. npn was determined in the soluble fraction, after protein precipitation with 24% trichloroacetic acid and centrifugation. digestibility was assessed by digestion with pepsin and pancreatin, and defined as the increase in non-protein n after enzymatic digestion. values for true protein were from 1.3 to 2.3 g/dl and for non protein n, from 4.5 to 13.7%. digestibility values varied between 59.0 and 92.5%; an inverse trend was observed between protein digestibility and protein content. considering both the "true protein" levels and their digested proportions, all preterm and 60% of the term formulas would present potentially available amounts below those recommended. these observations constitute an alert, even though this method of assessing "in vitro" protein digestibility represents only an approximation to physiological processes; however, it could be useful in order to evaluate the intensity of the heat treatments to which these formulas were subjected. on the other hand, since npn allows the estimation of the true protein provided by the formulas, either its percentage or the true protein content could be included on the label
Propiedades físicas de algunos suelos de Cuba y su uso en modelos de simulación
Cid,Greco; López,Teresa; González,Felicita; Herrera,Julián; Elena Ruiz,María;
Revista Ciencias T??cnicas Agropecuarias , 2011,
Abstract: today it is very common to use simulation models in agriculture, in order to estimate, for a wide range of natural environments, the behavior of some variables in one way or another to predict a specific performance. these models usually include complex mathematical models in software and may come to predict the impact that could result in a system of land management on water in particular and the environment in general. moreover, estimation of the global climate change effect on the ground is also possible through these tools. but they require input data that sometimes is not readily available as in the case of the physical properties of soils, and within these, those related to water operation. sometimes data does exist but because of the method used in collection and the way they are expressed, their use in simulation models leads to results that are far from what actually occurs in the natural environment being studied. the study shows the most used physical properties in some of the simulation models which have been used in our country as well as their manner of being expressed and the recommended method for its determination in the field.
Dise o de panes funcionales a base de harinas no tradicionales Design of functional breads based on non-traditional flours
ángela Zuleta,María Julieta Binaghi,Carola Beatriz Greco,Cristina Aguirre
Revista Chilena de Nutricíon , 2012,
Abstract: Los panes funcionales representan una alternativa interesante como vehículo de nuevas fuentes de fibra. Se estudiaron materias primas cuya obtención y uso es aún de tipo artesanal: la harina de banana verde (HBV) (Musa acuminata var nanica) y la harina de algarroba (HA) (Prosopis alba). Se elaboraron panes en mezcla con harina de trigo (HT): HBV/HT 50:50 y HA/HT 25:75. La composición centesimal de todas las muestras se realizó según AOAC. Se determinó el contenido de hierro, zinc y calcio por EAA y la dializabilidad mineral por un método in vitro. Tanto las materias primas como los panes se caracterizaron por un alto contenido de fibra (12,4 y 31,0 para HBV y HA y 6,9 y 10,2 para los panes de harina de plátano y algarroba respectivamente). Los panes presentaron alrededor de 22% menos de carbohidratos disponibles que el pan blanco. La dializabilidad de minerales se incrementó en los panes entre un 30 y un 50% ya que la fermentación promueve la degradación de los fitatos. El mayor efecto se observó sobre la dializabilidad de hierro. Estos resultados se alan a estas harinas como ingredientes promisorios para su utilización en el dise o de panes funcionales, con un importante aporte de fibra dietaria, que permite considerarlos alimentos ricos en fibra, de acuerdo a la legislación vigente (Código Alimentario Argentino) que se ala un mínimo de 6% para dichos productos. La inclusión de HA contribuyó a un mayor aporte de hierro y calcio, mientras que la de HBV, por sus características funcionales, permitió un mayor reemplazo de harina de trigo. Functional breads constitute an interesting alternative as a vehicle of new dietary fiber sources. Two ingredients which are obtained and used at regional levels were studied: green banana flour (GBF) (Musa acuminata var nanica) and American carob flour (CF) (Prosopis alba). Breads were made mixing these flours with wheat flour (WF): GBF/WF 50:50 and CF/WF 25:75. Proximal composition of all samples was assessed according to AOAC methods. Iron, zinc and calcium contents were determined by AAS and mineral dialyzability using an in vitro method. The studied flours as well as the breads were characterized by high dietary fiber contents (12.4 and 31.0 in GBF and CF; and 6.9 and 10.2 in the green banana and carob breads, respectively). Breads had about 22% less available carbohydrates than white bread. Mineral dialyzability increased in breads about 30 to 50%, since fermentation promotes phytates degradation. The greatest effect was observed on iron dialyzability. These results indicate that these flours might be promising ing
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