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Search Results: 1 - 10 of 479268 matches for " María Teresa; Cruz Sui "
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Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
OTTO CRUZ SUI,MARíA TERESA PéREZ GUEVARA,MARICELA IZQUIERDO MáRQUEZ,LEONOR LOBAINA BATELEMY
Revista Cubana de Medicina Tropical , 1997,
Abstract: Se reporta la evaluación del sistema DAVIH-BLOT (Laboratorios DAVIH, Cuba) frente a los paneles de proficiencia enviados al Laboratorio Nacional de Referencia del SIDA, por la Organización Mundial de la Salud (OMS), para el aseguramiento de la calidad en el diagnóstico de anticuerpos al VIH en los a os 1989, 1991 y 1993, así como la comparación del sistema con los de las firmas DuPont y Diagnostic Pasteur en un grupo de 467 sueros positivos por ELISA. En el estudio de las muestras de la OMS el sistema demostró ser altamente sensible y específico. Al compararlos con los estuches HIV-1 Western blot IgG versión 1.2 (DuPont Company, EE.UU.) y New LAV-blot 1 (Diagnostic Pasteur, Francia) tuvo una coincidencia del 100 y 97 %, respectivamente. It is reported the evaluation of the DAVIH-BLOT system (DAVIH Laboratories, Cuba) against the proficiency panels sent to the National AIDS Reference Laboratory by the World Health Organization (WHO) in order to ensure the quality in the diagnosis of HIV antibodies during 1989, 1991 and 1993. The system is also compared with those of the Dupont and Diagnostic Pasteur firms in a group of 467 positive sera by ELISA. On studying the WHO's samples, the system proved to be highly sensitive and specific. In the comparison made with the HIV-1 Western Blot IgG version 1.2 (Dupont company, USA) and with the LAV-blot 1 (Diagnostic Pasteur, France), there was a coincidence of 100 and 97 %, respectively.
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1
Cruz Sui,Otto; Pérez Guevara,. María Teresa; Lubián Caballero,Ana Luisa; Montano Tamayo,Lucy; Silva Cabrera,Eladio;
Revista Cubana de Medicina Tropical , 2007,
Abstract: the objective of this report was to evaluate oral fluid and urine as optional samples for hiv-1 confirmatory test using davih-blot system (laboratorios davih, la habana, cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. in band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. these results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for hiv-1 antibody confirmation testing.
Fluido oral y orina como muestras alternativas en el diagnóstico confirmatorio de la infección por VIH-1 Oral fluid and urine as alternative samples in confirmatory testing of HIV-1 infection
Otto Cruz Sui,. María Teresa Pérez Guevara,Ana Luisa Lubián Caballero,Lucy Montano Tamayo
Revista Cubana de Medicina Tropical , 2007,
Abstract: Se evaluó el fluido oral y la orina como muestras opcionales en el diagnóstico confirmatorio del VIH-1 con el sistema DAVIH-BLOT (Laboratorios DAVIH, La Habana, Cuba), para lo cual se comparó con las correspondientes muestras de sueros en un grupo de 125 individuos. En el análisis de los patrones de bandas reveladas, los anticuerpos predominantes en las muestras positivas de fluido oral y orina contra la p34, p68, gp41 y gp120, no tuvieron diferencias con sus correspondientes sueros según la prueba de comparación de proporciones (p< 0,001). La sensibilidad y especificidad relativas del sistema resultaron de 100 % en el análisis del fluido oral; 98,75 y 100 %, respectivamente, en las muestras de orina. Estos resultados apoyan el empleo opcional del fluido oral y la orina que, con ligeras modificaciones introducidas en el sistema diagnóstico, pueden aplicarse para la confirmación de anticuerpos contra el VIH-1. The objective of this report was to evaluate oral fluid and urine as optional samples for HIV-1 confirmatory test using DAVIH-BLOT system (Laboratorios DAVIH, La Habana, Cuba), for which they were compared with their corresponding serum samples in a group of 125 individuals. In band pattern analysis, predominant antibodies in positives oral fluid and urine samples against p34, p68, gp41, gp120, were no different from their corresponding sera according to the proportion comparison test (p<0,001). Relative sensitivity and specificity of this system were 100 % in oral fluid and 98, 75% and 100% in urine samples respectively. These results support optional use of oral fluid and urine that, with slight modifications in the diagnostic system, can be applied for HIV-1 antibody confirmation testing.
Determinación de anticuerpos al virus de la inmunodeficiencia humana tipo 1 (VIH-1) en muestras de sangre seca en papel de filtro
María Teresa Pérez Guevara,Felipe M. Rolo Gómez,Carmen Nibot Sánchez,Otto Cruz Sui
Revista Cubana de Medicina Tropical , 1998,
Abstract: Se estudia la posibilidad de detectar anticuerpos al VIH-1 mediante un estuche de Immunoblotting, en un papel de 125 muestras conocidas de sangre seca colectadas en papel de filtro y su correspondiente muestra de suero. No se apreciaron diferencias en los patrones de bandas con ambos tipos de muestras, así como en la sensibilidad y especificidad, donde se alcanzaron cifras del 100 %, lo que permitió concluir que la muestra de sangre tomada en papel de filtro puede ser empleada para la detección de anticuerpos al VIH-1 por el sistema DAVIH-BLOT y puede ser conservada a 4 oC por 30 semanas. The possibility of detecting HIV-1 antibodies by an inmunoblotting kit is studied in a panel of 125 known specimens of dried blood spotted on filter paper and their corresponding serum samples. No differences were observed in the patterns of bands with both types of samples or in the sensitivity and specificity, where 100 % figures were attained, allowing to conclude that the blood specimen taken on filter paper may be used for the detection of HIV-1 antibodies by the DAVIH-BLOT system and may be kept at 4 oC during 30 weeks.
Determinación de anticuerpos al virus de la inmunodeficiencia humana tipo 1 (VIH-1) en muestras de sangre seca en papel de filtro
Pérez Guevara,María Teresa; Rolo Gómez,Felipe M.; Nibot Sánchez,Carmen; Cruz Sui,Otto; Rodríguez,Orfelina;
Revista Cubana de Medicina Tropical , 1998,
Abstract: the possibility of detecting hiv-1 antibodies by an inmunoblotting kit is studied in a panel of 125 known specimens of dried blood spotted on filter paper and their corresponding serum samples. no differences were observed in the patterns of bands with both types of samples or in the sensitivity and specificity, where 100 % figures were attained, allowing to conclude that the blood specimen taken on filter paper may be used for the detection of hiv-1 antibodies by the davih-blot system and may be kept at 4 oc during 30 weeks.
Evaluación de un sistema de Western Blot (DAVIH-BLOT) para la confirmación de anticuerpos al VIH-1
CRUZ SUI,OTTO; PéREZ GUEVARA,MARíA TERESA; IZQUIERDO MáRQUEZ,MARICELA; LOBAINA BATELEMY,LEONOR; RUIBAL BRUNET,IGNACIO; SILVA CABRERA,ELADIO;
Revista Cubana de Medicina Tropical , 1997,
Abstract: it is reported the evaluation of the davih-blot system (davih laboratories, cuba) against the proficiency panels sent to the national aids reference laboratory by the world health organization (who) in order to ensure the quality in the diagnosis of hiv antibodies during 1989, 1991 and 1993. the system is also compared with those of the dupont and diagnostic pasteur firms in a group of 467 positive sera by elisa. on studying the who's samples, the system proved to be highly sensitive and specific. in the comparison made with the hiv-1 western blot igg version 1.2 (dupont company, usa) and with the lav-blot 1 (diagnostic pasteur, france), there was a coincidence of 100 and 97 %, respectively.
Seroprevalencia de la infección por HTLV-1 en diferentes grupos de riesgo estudiados en Cuba
Lubián Caballero,Ana Luisa; Díaz Torres,Héctor Manuel; Silva Cabrera,Eladio; Pérez Guevara,María Teresa; Cruz Sui,Otto; de la Fuente Arzola,José Luis; Navea Leyva,Leonor; Noa Romero,Enrique;
Revista Cubana de Medicina , 1998,
Abstract: in the period between 1991 and 1996, 26 352 serum samples from different risk groups and blood donors, were studied to know the seroprevalence of antibodies against the human t-lymphotropic virus type i (htlv-i), and also as a continuation of researches carried out between 1989 and 1990. the elisa davih-htlv-i system was used for the antibodies investigation, and as a certifying test, the western blot davih-blot htlv-i was used too, both from the cuban davih laboratories. the presence of anti htlv-i antibodies was confirmed in 10 persons, and in most of them the epidemiologic study could elucidate the probable way of contagion. the seropositivity index observed was of 0,037 %, and is low if compared with the seroprevalence rates reported for the caribbean area.
Biotechnology and Conservation of Plant Biodiversity
Carlos Alberto Cruz-Cruz,María Teresa González-Arnao,Florent Engelmann
Resources , 2013, DOI: 10.3390/resources2020073
Abstract: Advances in plant biotechnology provide new options for collection, multiplication and short- to long-term conservation of plant biodiversity, using in vitro culture techniques. Significant progress has been made for conserving endangered, rare, crop ornamental, medicinal and forest species, especially for non-orthodox seed and vegetatively propagated plants of temperate and tropical origin. Cell and tissue culture techniques ensure the rapid multiplication and production of plant material under aseptic conditions. Medium-term conservation by means of in vitro slow growth storage allows extending subcultures from several months to several years, depending on the species. Cryopreservation (liquid nitrogen, ?196 °C) is the only technique ensuring the safe and cost-effective long-term conservation of a wide range of plant species. Cryopreservation of shoot tips is also being applied to eradicate systemic plant pathogens, a process termed cryotherapy. Slow growth storage is routinely used in many laboratories for medium-conservation of numerous plant species. Today, the large-scale, routine application of cryopreservation is still restricted to a limited number of cases. However, the number of plant species for which cryopreservation techniques are established and validated on a large range of genetically diverse accessions is increasing steadily.
Detección de los genes de las exotoxinas pirogénicas SpeA, SpeB y SpeC en cepas chilenas de Streptococcus pyogenes y su asociación con la clínica Detection of pyrogenic exotoxin SpeA, SpeB and SpeC genes in Chilean streptococci isolates and their association with clinical manifestations
María Teresa Ulloa F,María Soledad Giglio M,Lorena Porte T,Adriana Santa Cruz A
Revista médica de Chile , 2000,
Abstract: Background: The virulence of Streptococcus pyogenes is determined by a variety of structural molecules, toxins and complex enzymes. Pyrogenic exotoxins cause fever, erythematous reactions, cytotoxic and immunological effects. Aim: To assess the frequency of speA, speB and speC genes in Chilean Streptococcus pyogenes strains and their association with the invasiveness of infections. Material and methods: The genes for pyrogenic exotoxins SpeA, SpeB and SpeC were determined by polymerase chain reactions in 114 strains of group A Streptococcus pyogenes isolated from Chilean patients with invasive or non invasive infections. Results: The gene for SpeA was present in 30.7% of isolates, the gene for SpeB was present in 69.3% and the gen for SpeC in 44.7% of isolates. The gene for SpeA was present in 20 of 33 invasive infections and in 15 of 81 non invasive infections (p <0.0001). On the contrary, the gene for SpeC was present in 11 of 33 invasive infections and in 41 of 81 non invasive infections (p <0.05). The frequency of speB was similar in invasive and non invasive infections. Conclusions: There is a clear relationship between the presence of SpeA genes and the severity of infections caused by Streptococcus pyogenes. (Rev Méd Chile 2000; 128: 27-34)
Impacto económico por el pago de pasajeros de cruceros para la conservación de recursos naturales: el caso de Bahías de Huatulco, México
María Teresa Kido-Cruz,Manuel Cuéllar-Río
Economía, sociedad y territorio , 2010,
Abstract: Bahías de Huatulco, México, es un destino turístico relativamente joven y con gran atractivo por su belleza escénica, pero se ve amenazado por la llegada masiva de pasajeros de cruceros cada a o, por lo que resulta primordial crear instrumentos económicos e implementar políticas que minimicen el da o a los recursos naturales de la zona. En este estudio se utiliza el método del costo de viaje y un modelo probit binomial que utiliza datos de panel para analizar el impacto económico causado por el turista de cruceros, así como su disposición a pagar por la conservación de los recursos naturales. Los resultados principales muestran un excedente del consumidor de 48 dólares por persona, pero también indican que 30% de los pasajeros no estaría dispuesto a pagar un incremento en la cuota de entrada al puerto.
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