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Search Results: 1 - 10 of 48 matches for " Maldaner Joseila "
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Otimiza??o da micropropaga??o de Pfaffia tuberosa (Spreng.) Hicken
Flores, Rejane;Maldaner, Joseila;Nicoloso, Fernando Teixeira;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000300018
Abstract: this work aimed at optimizing the micropropagation protocol of pfaffia tuberosa, a medicinal species found in several regions of brazil. the treatment of donor plants with benomyl (1%), successively followed with solutions of commercial bleach (2 drops/100ml water; for 2 min), ethanol (70%; for 10 seconds), sodium hypochloride (1%) + commercial bleach (2 drops/100ml water; for 10 minutes) and hgcl2 (0.1%; for 5 min) on the disinfection step of explants were suitable for disinfection and plant regeneration. in vitro plantlets must be subcultivated to a new ms medium at 30 days intervals. explants from nodal segments showed a higher multiplication rate when compared to apical segments. a decline was not observed on the propagation rate during five successive subcultives in vitro. plantlets showed an elevated index of survival in the acclimatization step.
Otimiza o da micropropaga o de Pfaffia tuberosa (Spreng.) Hicken
Flores Rejane,Maldaner Joseila,Nicoloso Fernando Teixeira
Ciência Rural , 2006,
Abstract: Este trabalho objetivou otimizar o protocolo de micropropaga o de Pfaffia tuberosa, uma espécie medicinal encontrada em várias regi es do Brasil. O tratamento da planta matriz com benomyl (0,1%) e o uso sucessivo de solu es com detergente comercial (duas gotas/100mL de água, durante dois minutos), etanol (70%, por 10 segundos), hipoclorito de sódio (1%) + detergente comercial (duas gotas/100mL de água, por 10 minutos) e HgCl2 (0,1%, por 5 minutos) na etapa de desinfesta o dos explantes foram eficazes para a limpeza e regenera o de plantas. As plantas devem ser subcultivadas para um novo meio MS a cada 30 dias de cultivo. Explantes provenientes de segmentos nodais apresentaram uma maior taxa de multiplica o quando comparados com os apicais. N o foi observado declínio na taxa de propaga o no decorrer de cinco subcultivos in vitro. As mudas apresentaram um índice elevado de sobrevivência durante a fase de aclimatiza o.
Substratos na aclimatiza??o de Pfaffia glomerata (Spreng) Pedersen produzida in vitro sob diferentes doses de sacarose
Skrebsky, Etiane Caldeira;Nicoloso, Fernando Teixeira;Maldaner, Joseila;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000500011
Abstract: this work was aimed at selecting substrates on the ex vitro acclimatization of pfaffia glomerata produced in vitro under different sucrose levels. the treatments consisted of a bifactorial combination (5x3) between five sucrose levels (15, 30, 45, 60, and 75g l-1), present in the in vitro culture, and three substrates [plantmax?; plantmax? + soil (1:1 v/v), and vermiculite (middle size) + soil (paleudalf) (1:1 v/v)] used in the ex vitro acclimatization steps. physical and chemical evaluations of the substrates were carried out as well as evaluations of plant growth and survival for both in vitro and ex vitro cultures. plants obtained from in vitro cultures in the presence of 45 to 60g l-1 sucrose showed better ex vitro acclimatization. the substrate combinations vermiculite + soil and plantmax? + soil gave rise greater growth to plants during the last acclimatization step (partially shady natural environment), probably due to having greater total porosity. however, the isolated use of plantmax? increased the plant survival under field conditions because this substrate showed the greatest values for water retention capacity, easily available water and available water.
Substratos na aclimatiza o de Pfaffia glomerata (Spreng) Pedersen produzida in vitro sob diferentes doses de sacarose
Skrebsky Etiane Caldeira,Nicoloso Fernando Teixeira,Maldaner Joseila
Ciência Rural , 2006,
Abstract: Este trabalho teve como objetivo selecionar substratos para a aclimatiza o de plantulas de Pfaffia glomerata produzidas in vitro sob diferentes concentra es de sacarose. Os tratamentos consistiram de uma combina o bifatorial (5x3) entre cinco doses de sacarose (15, 30, 45, 60 e 75g L-1), presentes no meio de cultura in vitro, e três substratos [Plantmax Hortali as, Plantmax + Solo (1:1 v/v) e Vermiculita (granulometria média) + Solo (ARGISSOLO VERMELHO Distrófico arênico) (1:1 v/v)] utilizados na aclimatiza o ex vitro. Foram realizadas determina es das características físicas e químicas dos substratos, bem como avalia es do crescimento e da sobrevivência das plantas tanto durante o cultivo in vitro como no ex vitro. Plantas provenientes do cultivo in vitro na presen a de 45 a 60g L-1 de sacarose apresentaram melhor aclimatiza o ex vitro. As combina es dos substratos Vermiculita + solo (1:1 v/v) e Plantmax + solo (1:1 v/v) proporcionaram maior crescimento às plantas durante a última fase de aclimatiza o (cultivo sob sombrite), provavelmente devido a possuírem maior porosidade total. Entretanto, o uso isolado de Plantmax aumentou a sobrevivência das plantas cultivadas a campo, fato relacionado a esse substrato possuir os maiores valores de capacidade de reten o de água, de água facilmente disponível e de água disponível.
Extra??o de ecdisterona em raízes de ginseng brasileiro
Flores, Rejane;Nicoloso, Fernando Teixeira;Brondani, Daniela;Maldaner, Joseila;Cezarotto, Verciane;Giacomelli, Sandro Rogério;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000400042
Abstract: this study aimed at optimizing the extraction method from ecdysterone of brazilian ginseng. root samples of two accessions (bra and jb-ufsm) of p. glomerata were extracted in a soxhlet with methanol or chloroform for 4h. in the second trial, the extration was conduced in a soxhlet or ultrasonic using metanol as a solvent. in p. tuberosa, the roots samples were extracted with methanol in a soxhlet or in ultrasonic. the ecdysterone content was determinated using high efficiency liquid chromatography methods. in both studied species, the highest ecdisterone content was detected from samples extracted in a soxhlet and using methanol as a solvent. this extration method has been successfully applied for determination of ecdysterone content from roots of brazilian ginseng, and could be useful for the quality control of drugs and pharmaceutical formulations.
Sacarose e nitrogênio na multiplica??o in vitro de Pfaffia glomerata (Spreng.) Pedersen
Maldaner, Joseila;Nicoloso, Fernando Teixeira;Santos, Eneide Schutz dos;Flores, Rejane;Skrebsky, Etiane Caldeira;
Ciência Rural , 2006, DOI: 10.1590/S0103-84782006000400024
Abstract: pfaffia glomerata (spreng.) pedersen, known as brazilian ginseng, is an extensively used plant in folk medicine due to its phytotherapic characteristics. this work aimed to determine the best combination of sucrose (30, 45 and 60 g l-1) and nitrogen (50, 75, 100 and 125% of the strength of ms medium) on micropropagation of p. glomerata. in 30 days of cultivation, sucrose ranging from 40 to 45g l-1 and nitrogen at 50% increased the height and the number of nodes. the number of shoots was greater at concentration of 55g l-1 sucrose combined with 70% n. the dry matter of roots, aerial parts, and of the whole seedling was increased on 50% n and 45g l-1 sucrose. altogether the halved concentration of nitrogen and the increased concentration of sucrose to 45g l-1 increase the height, the number of nodes and the shoots as well as biomass production improved due to an enhancemednt of carbon use.
Sacarose e nitrogênio na multiplica o in vitro de Pfaffia glomerata (Spreng.) Pedersen
Maldaner Joseila,Nicoloso Fernando Teixeira,Santos Eneide Schutz dos,Flores Rejane
Ciência Rural , 2006,
Abstract: Pfaffia glomerata (Spreng.) Pedersen, conhecida como ginseng brasileiro, é uma planta extensivamente usada na medicina popular em decorrência de possuir propriedades fitoterápicas. Este trabalho teve como objetivo determinar a melhor combina o de concentra o da sacarose (30, 45 e 60g L-1) e do nitrogênio (50, 75, 100 e 125% da concentra o padr o do meio MS) para a multiplica o in vitro de P. glomerata. Aos 30 dias de cultivo, verificou-se que concentra es entre 40 e 45g L-1 de sacarose e 50% de N propiciaram maior crescimento em altura e número de segmentos nodais por plantula. O número de brota es foi maior na concentra o de 55g L-1 de sacarose combinada com 70% de N. A matéria seca de raízes, da parte aérea e do total da plantula foi maior na concentra o de 45g L-1 de sacarose associada com 50% de N. No geral, a redu o da concentra o de N para 50% daquela padr o do meio MS, associada a um incremento na dose de sacarose para 45g L-1, favorece o crescimento em altura, número de segmentos nodais e brota es, bem como a produ o de biomassa de P. glomerata cultivada in vitro, devido ao estímulo ao uso do carbono.
Crescimento de plantulas de Pfaffia glomerata (Spreng.) Pedersen cultivadas in vitro sob dois níveis de nitrogênio e sacarose, durante seis subculturas sucessivas e aclimatiza??o
Maldaner, Joseila;Nicoloso, Fernando Teixeira;Santos, Eneide Schutz dos;Fagundes, Camila Kurzmann;Flores, Rejane;Jucoski, Gládis de Oliveira;Skrebsky, Etiane Caldeira;
Ciência Rural , 2007, DOI: 10.1590/S0103-84782007000100021
Abstract: the present research was aimed at evaluating the growth of brazilian ginseng (pfaffia glomerata (spreng.) pedersen) in vitro cultured plantlets under two levels of nitrogen and sucrose, during six successive subcultures and acclimatization. two nutritional treatments were tested: nitrogen at 100% strength of the ms medium + 30g l-1 sucrose; and nitrogen at 50% strength + 45g l-1 sucrose. during six successive subcultures, the explants of 30-day-old plantlets grown under both treatments were transferred, respectively to the next subculture on the same treatment conditions. at 6th subculture, plantlets were transferred to substrate plantmaxa hortali?as and submitted to acclimatization in a growth chamber. throughout the subcultures, fluctuations in growth response due to nutritional conditions tested were observed. the height of the higher sprout, the average height of sprouts, and the total number of nodal segments per plantlet, respectively for four, four and three subcultures were greater in 100% strength of the ms medium + 30g l-1 sucrose than in 50% strength + 45g l-1 sucrose. on the other hand, dry matter of roots, of shoots and of the whole plant, respectively for two, two and three subcultures, were greater in 50% strength + 45g l-1 sucrose. in conclusion, the increase of sucrose associated with reduction of n level in the culture medium enhanced the biomass production, while the ms at full strength enhanced the growth based in sprout length and number of nodal segments. these differences concerning the biomass production observed during in vitro culture did not persisted in the acclimatization process ex vitro, where ms at full strength give rise greater growth in sprout length and number of leaves per plant.
Crescimento in vitro de plantulas de batata em diferentes doses de cádmio
Gon?alves, Jamile Fabbrin;Maldaner, Joseila;Rossato, Liana Veronica;Tabaldi, Luciane Almeri;Skrebsky, Etiane Caldeira;Farias, Júlia Gomes;Bisognin, Dilson Ant?nio;Nicoloso, Fernando Teixeira;
Ciência Rural , 2009, DOI: 10.1590/S0103-84782009000900038
Abstract: levels of cadmium have been increasing in many agricultural soils mainly due to the anthropogenic actions, such as industrialization and use of inputs in the agriculture. the present research aimed at characterizing the effect of this metal on in vitro growth of two potato cultivars, 'asterix' and 'macaca'. nodal segments of plants previously in vitro established were submitted to cadmium concentration of 0 (control), 100, 200, 300, 400 and 500μm in ms. number of roots was evaluated at 15 days after inoculation (dai) of the explants, and root and shoot length, number of nodal segments and leaves, and fresh and dry mass of roots and shoot were evaluated at 22dai. cadmium negatively affected the growth of the two potato cultivars, demonstrating that both are sensible to this metal.
Caracteriza??o das exigências nutricionais de mudas de Pfaffia glomerata em Argissolo Vermelho distrófico arênico pela técnica do nutriente faltante
Skrebsky, Etiane Caldeira;Nicoloso, Fernando Teixeira;Maldaner, Joseila;Rauber, Renata;Castro, Gabriel Y;Jucoski, Gládis de Oliveira;Santos, Danilo Rheinheimer dos;
Ciência Rural , 2008, DOI: 10.1590/S0103-84782008000400013
Abstract: this study aimed to characterize the nutritional demands of the young plants of brazilian ginseng (pfaffia glomerata (spreng.) pedersen) in a paleudalf soil by means of the missing nutrient technique. plantlets of p. glomerata produced in vitro and acclimatized ex vitro were used as start matter for the glasshouse experiment. ten days after plant transfer to soil, fertilizations (by means of nutritive solutions) were done corresponding to nine treatments: control (without fertilization), complete fertilization (with macro and micronutrients), and with the omissions of n, p, k, ca, mg, s and micronutrients (zn, b, cu, fe, mn and mo). at 60 days of cultivation, several growth parameters were evaluated. the number of leaves per plant was the growth parameter most affected by omission of n, k, s and p in the fertilization. the effect of nutrient omission in the fertilization on biomass partitioning between plant organs were differentiated. accumulated shoot biomass obeied the following decreasing order, in relation with the nutrient omission: mg @ micronutrients > ca @ complete fertilization > p > s > k > n @ control. while the root biomass obeied the following decreasing order: mg @ complete fertilization 3 p 3 micronutrients 3 s 3 ca > n > k @ control. young plants of p. glomerata grow in a paleudalf soil showed greater reduction in growth by omission of n, k, s and p in the fertilization. the omission of mg and micronutrients in the fertilization were not limiting to the growth of young plants.
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